{"id":43549,"date":"2020-10-21T00:00:00","date_gmt":"2020-10-21T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/japanese-improve-acrylamide-analysis-in-food-with-a-long-lasting-lc-column-and-a-cost-effective-internal-standard\/"},"modified":"2026-01-14T19:14:02","modified_gmt":"2026-01-14T19:14:02","slug":"japanese-improve-acrylamide-analysis-in-food-with-a-long-lasting-lc-column-and-a-cost-effective-internal-standard","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/articles-ja\/ffar3126-ja\/japanese-improve-acrylamide-analysis-in-food-with-a-long-lasting-lc-column-and-a-cost-effective-internal-standard","title":{"rendered":"\u8010\u4e45\u6027\u306b\u512a\u308c\u305fLC\u30ab\u30e9\u30e0\u3068\u8cbb\u7528\u5bfe\u52b9\u679c\u306e\u9ad8\u3044\u5185\u90e8\u6a19\u6e96\u306b\u3088\u308b\u98df\u54c1\u4e2d\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u5206\u6790\u306e\u6539\u5584"},"content":{"rendered":"\n<p>\u713c\u304f\u3001\u7092\u3081\u308b\u3001\u63da\u3052\u308b\u3001\u306a\u3069\u9ad8\u71b1\u8abf\u7406\u306e\u904e\u7a0b\u3067\u7cd6\u985e\u3068\u30a2\u30df\u30ce\u9178\u3067\u3042\u308b\u30a2\u30b9\u30d1\u30e9\u30ae\u30f3\u304c\u53cd\u5fdc\u3059\u308b\u3068\u3001\u98df\u54c1\u4e2d\u306b\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u304c\u5f62\u6210\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u306e\u767a\u751f\u3084\u6fc3\u5ea6\u304c\u304b\u304b\u308f\u3063\u3066\u304f\u308b\u5546\u54c1\u4f8b\u3068\u3057\u3066\u306f\u3001\u30dd\u30c6\u30c8\u30c1\u30c3\u30d7\u30b9\u3001\u7a40\u985e\u3092\u539f\u6599\u3068\u3059\u308b\u713c\u304d\u83d3\u5b50\u3001\u30b3\u30fc\u30d2\u30fc\u306a\u3069\u304c\u3042\u3052\u3089\u308c\u307e\u3059\u3002\u98df\u54c1\u4e2d\u306e\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u306e\u691c\u51fa\u306f\u3001\u30b9\u30a6\u30a7\u30fc\u30c7\u30f3\u56fd\u7acb\u98df\u54c1\u5e81\u306b\u3088\u3063\u30662002\u5e74\u306b\u521d\u3081\u3066\u5831\u544a\u3055\u308c\u3001\u305d\u308c\u4ee5\u6765\u3001\u98df\u54c1\u3084\u98fc\u6599\u306b\u7531\u6765\u3059\u308b\u3055\u307e\u3056\u307e\u306a\u98df\u54c1\u306e\u6c5a\u67d3\u898f\u6a21\u3084\u3001\u98df\u4e8b\u304b\u3089\u306e\u66b4\u9732\u306b\u95a2\u9023\u3059\u308b\u6f5c\u5728\u7684\u306a\u5065\u5eb7\u5f71\u97ff\u3092\u3088\u308a\u7406\u89e3\u3059\u308b\u305f\u3081\u306e\u4e16\u754c\u7684\u306a\u53d6\u308a\u7d44\u307f\u304c\u62e1\u5927\u3057\u3066\u3044\u307e\u3059\u3002\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u306f\u975e\u5e38\u306b\u5371\u967a\u306a\u5316\u5b66\u7269\u8cea\u3068\u3057\u3066\u5206\u985e\u3055\u308c\u3066\u304a\u308a\u3001\u5b9f\u9a13\u52d5\u7269\u3067\u306f\u5927\u91cf\u6295\u4e0e\u306b\u3088\u308a\u304c\u3093\u3092\u5f15\u304d\u8d77\u3053\u3059\u53ef\u80fd\u6027\u304c\u6307\u6458\u3055\u308c\u3066\u3044\u307e\u3059\uff08\u305f\u3060\u3057\u3001\u3053\u308c\u307e\u3067\u306e\u3068\u3053\u308d\u3001\u98df\u4e8b\u306b\u3088\u308b\u66b4\u9732\u3068\u30d2\u30c8\u306e\u764c\u767a\u751f\u7387\u306e\u5897\u52a0\u3068\u306e\u76f4\u63a5\u7684\u306a\u95a2\u4fc2\u306f\u8a3c\u660e\u3055\u308c\u3066\u3044\u307e\u305b\u3093\uff09\u3002\u516c\u8846\u885b\u751f\u7dad\u6301\u306e\u305f\u3081\u306b\u3001\u98df\u54c1\u3084\u98f2\u6599\u4e2d\u306e\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u6fc3\u5ea6\u3092\u6b63\u78ba\u306b\u6e2c\u5b9a\u3067\u304d\u308b\u3001\u52b9\u7387\u7684\u306a\u6e2c\u5b9a\u6cd5\u304c\u5fc5\u8981\u3068\u3055\u308c\u307e\u3059\u3002\u3053\u3053\u3067\u306f\u3001\u98df\u54c1\u4e2d\u306e\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u5206\u6790\u306b\u5bfe\u3059\u308b\u4ee3\u8868\u7684\u306a\u30a2\u30d7\u30ed\u30fc\u30c1\u3092\u3001Allure Acrylamide\u30ab\u30e9\u30e0\u3068\u91cd\u6c34\u7d20\u5316\u5185\u90e8\u6a19\u6e96\u3092\u4f7f\u7528\u3059\u308b\u6539\u5584\u3055\u308c\u305f\u30d7\u30ed\u30bb\u30b9\u3068\u6bd4\u8f03\u3057\u307e\u3059\u3002\u5206\u6790\u6642\u9593\u306e\u77ed\u7e2e\u3068\u30b7\u30b9\u30c6\u30e0\u9069\u5408\u6027\u3092\u9038\u8131\u3059\u308b\u3053\u3068\u306e\u306a\u3044\u9577\u671f\u9593\u306b\u308f\u305f\u308b\u30b5\u30f3\u30d7\u30eb\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u3082\u5229\u70b9\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u73fe\u884c\u306e\u5206\u6790\u624b\u6cd5<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\">LC-MS \/ MS\u6cd5\u306e\u9078\u629e<\/h3>\n\n\n\n<p>\u98df\u54c1\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u4e2d\u306e\u6b63\u78ba\u306a\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u5206\u6790\u306b\u306f\u3001\u6280\u8853\u7684\u306a\u8ab2\u984c\u304c\u3042\u308a\u307e\u3059\u3002\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u5206\u5b50\u306f\u5c0f\u3055\u304f\u3001\u6bd4\u8f03\u7684\u63ee\u767a\u6027\u304c\u9ad8\u3044\u5316\u5408\u7269\u3067\u3042\u308b\u305f\u3081\u3001\u5f53\u521d\u306fGC\/MS\u306b\u3088\u308b\u624b\u6cd5\u304c\u8ffd\u6c42\u3055\u308c\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3053\u308c\u3089\u306e\u624b\u6cd5\u306f\u3001\u671b\u307e\u3057\u304f\u306a\u3044\u30b9\u30c6\u30c3\u30d7\u3067\u3042\u308b\u3001\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u306e\u81ed\u7d20\u5316\u8a98\u5c0e\u4f53\u3078\u306e\u5909\u63db\u306b\u4f9d\u5b58\u3059\u308b\u3082\u306e\u3067\u3057\u305f[1]\u3002\u305d\u306e\u5f8c\u3001LC-MS\/MS\u3092\u7528\u3044\u305f\u624b\u6cd5\u304c\u7814\u7a76\u3055\u308c\u3001\u73fe\u5728\u3067\u306f\u98df\u54c1\u30b5\u30f3\u30d7\u30eb\u306e\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u5206\u6790\u306b\u65e5\u5e38\u7684\u306b\u4f7f\u7528\u3055\u308c\u308b\u6700\u3082\u4e00\u822c\u7684\u306a\u5206\u6790\u6cd5\u3068\u306a\u308a\u307e\u3057\u305f\u3002LC-MS\/MS\u6cd5\u306f\u4f9d\u7136\u3068\u3057\u3066\u5206\u6790\u306e\u30cf\u30fc\u30c9\u30eb\u306b\u76f4\u9762\u3057\u3066\u3044\u307e\u3059\u3002\u4e2d\u3067\u3082\u6ce8\u76ee\u3059\u3079\u304d\u306f\u3001\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u306e\u9078\u629e\u7684\u306a\u4fdd\u6301\u3068\u8907\u96d1\u306a\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u304b\u3089\u5171\u62bd\u51fa\u3055\u308c\u305f\u5316\u5408\u7269\u304b\u3089\u306e\u5206\u96e2\u3067\u3001\u73fe\u5728\u3082\u7814\u7a76\u304c\u9032\u3081\u3089\u308c\u3066\u3044\u307e\u3059\u3002&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u591a\u5b54\u8cea\u30b0\u30e9\u30d5\u30a1\u30a4\u30c8\u30ab\u30fc\u30dc\u30f3\uff08PGC\uff09LC\u30ab\u30e9\u30e0\u306e\u4f7f\u7528<\/h3>\n\n\n\n<p>\u30e8\u30fc\u30ed\u30c3\u30d1\u3067\u306f\u3001\u98df\u54c1\u4e2d\u306e\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u306e\u5206\u6790\u65b9\u6cd5\u304c\u6b63\u5f0f\u306b\u5b9a\u3081\u3089\u308c\u3066\u304a\u308a\uff08EN 16618\uff1a2015\uff09\u3001\u591a\u5b54\u6027\u30b0\u30e9\u30d5\u30a1\u30a4\u30c8\u30ab\u30fc\u30dc\u30f3\uff08PGC: porous graphitized carbon\uff09\u3092\u5145\u586b\u3057\u305f\u30ac\u30fc\u30c9\u30ab\u30e9\u30e0\u3068\u5206\u6790\u30ab\u30e9\u30e0\u306e\u4f7f\u7528\u304c\u6c42\u3081\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u306f\u3001\u5f93\u6765\u306e\u9006\u76f8\uff08RP\uff09\u4fdd\u6301\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u7528\u3044\u3066\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u3092\u5206\u6790\u3059\u308b\u3053\u3068\u304c\u96e3\u3057\u3044\u3053\u3068\u3082\u4e00\u56e0\u3067\u3059\u3002\u6bd4\u8f03\u7684\u53b3\u5bc6\u306aEN\u6cd5\u306b\u3088\u308b\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u624b\u9806\u3092\u884c\u3063\u305f\u5f8c\u3067\u3055\u3048\u3001\u5171\u62bd\u51fa\u3055\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u6210\u5206\u304b\u3089\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u3092\u5206\u96e2\u3059\u308b\u306e\u306b\u5145\u5206\u306a\u4fdd\u6301\u304c\u5f97\u3089\u308c\u308b\u305f\u3081\u3001PGC\u30ab\u30e9\u30e0\u306f\u73fe\u5728\u696d\u754c\u3067\u306f\u4e00\u822c\u7684\u306b\u4f7f\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002PGC\u30ab\u30e9\u30e0\u306f\u3001\u6c34\u7cfb100\uff05\u79fb\u52d5\u76f8\u3067\u4f7f\u7528\u53ef\u80fd\u3067\u3059\u3002\u3053\u306e\u6761\u4ef6\u3067\u306f\u591a\u304f\u306e\u9006\u76f8\u30ab\u30e9\u30e0\u304c\u300cDewetting\u300d\u3068\u547c\u3070\u308c\u308b\u4fdd\u6301\u6642\u9593\u304c\u6975\u7aef\u306b\u77ed\u304f\u306a\u308b\u73fe\u8c61\u3092\u8d77\u3053\u3057\u3001\u6709\u6a5f\u6eb6\u5a92\u306e\u5272\u5408\u306e\u9ad8\u3044\u79fb\u52d5\u76f8\u3067\u306e\u6642\u9593\u306e\u304b\u304b\u308b\u6d17\u6d44\u306b\u3088\u308b\u30ab\u30e9\u30e0\u518d\u751f\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u91cd\u6c34\u7d20\u5316\u5185\u90e8\u6a19\u6e96\u7269\u8cea\u306e\u4f7f\u7528<\/h3>\n\n\n\n<p>\u6b63\u78ba\u306a\u5b9a\u91cf\u304c\u5fc5\u8981\u4e0d\u53ef\u6b20\u3067\u3059\u304c\u3001\u62bd\u51fa\u30d7\u30ed\u30bb\u30b9\u3067\u306f\u304b\u306a\u308a\u306e\u3070\u3089\u3064\u304d\u304c\u751f\u3058\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u305f\u3081\u3001EN\u30e1\u30bd\u30c3\u30c9\u3067\u306f\u5185\u90e8\u6a19\u6e96\u7269\u8cea\u3092\u62bd\u51fa\u6eb6\u5a92\u3068\u5171\u306b\u30b5\u30f3\u30d7\u30eb\u3078\u6dfb\u52a0\u3059\u308b\u3053\u3068\u304c\u63a8\u5968\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u5206\u6790\u306b\u306f\u4e00\u822c\u306b13C\u6a19\u8b58\u5185\u90e8\u6a19\u6e96\u7269\u8cea\u3092\u4f7f\u7528\u3057\u307e\u3059\u304c\u3001EN\u30e1\u30bd\u30c3\u30c9\u3067\u306f\u4ee3\u308f\u308a\u306b\u91cd\u6c34\u7d20\u5316\u5185\u90e8\u6a19\u6e96\u7269\u8cea\u306e\u4f7f\u7528\u304c\u53ef\u80fd\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u300213C\u6a19\u8b58\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u3088\u308a\u3082\u5927\u5e45\u306b\u5b89\u4fa1\u306a\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9-d3\u306f\u5185\u90e8\u6a19\u6e96\u7269\u8cea\u3068\u3057\u3066\u512a\u308c\u3001\u304b\u3064\u8cbb\u7528\u5bfe\u52b9\u679c\u306e\u9ad8\u3044\u9078\u629e\u80a2\u3067\u3059\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u30e1\u30bd\u30c3\u30c9\u8981\u4ef6<\/h3>\n\n\n\n<p>EN16618:2015\u306b\u6e96\u62e0\u3057\u305f\u98df\u54c1\u4e2d\u306e\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u5206\u6790\u3067\u6c42\u3081\u3089\u308c\u308b\u5206\u6790\u6642\u9593\u306f8\u5206\u3067\u3059\u3002\u30b7\u30b9\u30c6\u30e0\u9069\u5408\u6027\u306e\u8981\u4ef6\u3092\u6e80\u305f\u3059\u305f\u3081\u306b\u306f\u3001\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u306f1.7\u5206\u3088\u308a\u5f8c\u306b\u6eb6\u51fa\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3055\u3089\u306b\u3001EN\u30e1\u30bd\u30c3\u30c9\u306b\u6982\u8aac\u3055\u308c\u3066\u3044\u308b2\u6bb5\u968eSPE\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u5de5\u7a0b\u306e\u5f8c\u3067\u3082\u30b5\u30f3\u30d7\u30eb\u62bd\u51fa\u6db2\u4e2d\u306b\u6b8b\u7559\u3057\u3066\u3044\u308b\u30de\u30c8\u30ea\u30af\u30b9\u6210\u5206\u3092\u30ab\u30e9\u30e0\u304b\u3089\u6d17\u3044\u51fa\u3059\u305f\u3081\u3001\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u6eb6\u51fa\u5f8c\u306b\u8ffd\u52a0\u6642\u9593\u304c\u5fc5\u8981\u3067\u3059\u3002\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u6210\u5206\u306fPGC\u30ab\u30e9\u30e0\u306b\u5f37\u304f\u4fdd\u6301\u3055\u308c\u308b\u305f\u3081\u3001\u5206\u6790\u6bce\u306b\u5145\u5206\u306a\u30d1\u30fc\u30b8\u3092\u884c\u308f\u306a\u3044\u3068\u30ab\u30e9\u30e0\u6027\u80fd\u304c\u4f4e\u4e0b\u3057\u3001\u30b7\u30b9\u30c6\u30e0\u9069\u5408\u6027\u306e\u8981\u4ef6\u3067\u3042\u308b\u4fdd\u6301\u6642\u95931.7\u5206\u3092\u6e80\u305f\u3059\u3053\u3068\u304c\u3067\u304d\u306a\u304f\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002&nbsp;<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u9006\u76f8\u5206\u6790\u306b\u3088\u308b\u30b5\u30f3\u30d7\u30eb\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u306e\u5411\u4e0a<\/h2>\n\n\n\n<p>PGC\u30ab\u30e9\u30e0\u306e\u4f7f\u7528\u3059\u308b\u30e9\u30dc\u3067\u306f\u30b7\u30b9\u30c6\u30e0\u9069\u5408\u6027\u306e\u95be\u5024\u306b\u8fd1\u3065\u304f\u3068\u3001\u30b7\u30b9\u30c6\u30e0\u9069\u5408\u6027\u57fa\u6e96\u3092\u6e80\u305f\u3059\u6027\u80fd\u306b\u5fa9\u5e30\u3059\u308b\u3053\u3068\u3092\u671f\u5f85\u3057\u3066\u9577\u6642\u9593\u306b\u304a\u3088\u3076\u30ab\u30e9\u30e0\u518d\u751f\u306e\u624b\u9806\u3092\u5b9f\u65bd\u3059\u308b\u304b\u3001\u30ac\u30fc\u30c9\u30ab\u30e9\u30e0\u3068\u5834\u5408\u306b\u3088\u3063\u3066\u306f\u5206\u96e2\u30ab\u30e9\u30e0\u3082\u4ea4\u63db\u3059\u308b\u304b\u306e\u9078\u629e\u306b\u76f4\u9762\u3057\u307e\u3059\u3002\u3069\u3061\u3089\u3082\u6642\u9593\u3068\u8cbb\u7528\u306e\u304b\u304b\u308b\u554f\u984c\u3067\u3042\u308a\u3001\u30b5\u30f3\u30d7\u30eb\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u304c\u6ede\u308a\u307e\u3059\u3002Allure Acrylamide \u30ab\u30e9\u30e0\u3078\u306e\u5207\u308a\u66ff\u3048\u306f\u512a\u308c\u305f\u4ee3\u66ff\u624b\u6bb5\u3067\u3059\u3002\u72ec\u81ea\u306e\u6975\u6027\u57fa\u3092\u7d44\u307f\u8fbc\u3093\u3060\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u3092\u4fdd\u6301\u3059\u308b\u9006\u76f8\u56fa\u5b9a\u76f8\u306f\u3001\u6c34\u7cfb100%\u79fb\u52d5\u76f8\u6761\u4ef6\u306b\u9069\u5408\u3057\u3001PGC\u30ab\u30e9\u30e0\u3088\u308a\u3082\u77ed\u3044\u5206\u6790\u6642\u9593\u3068\u9577\u3044\u30ab\u30e9\u30e0\u5bff\u547d\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002Allure Acrylamide\u30ac\u30fc\u30c9\u30ab\u30e9\u30e0\u304a\u3088\u3073\u5206\u96e2\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3059\u308b\u3068\u3001\u6700\u3082\u826f\u3044\u30d0\u30e9\u30f3\u30b9\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3064\u307e\u308a\u3001EN\u306e\u30b7\u30b9\u30c6\u30e0\u9069\u5408\u6027\u8981\u4ef6\u3092\u6e80\u305f\u3059\u5145\u5206\u306a\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u306e\u4fdd\u6301\u3092\u63d0\u4f9b\u3059\u308b\u4e00\u65b9\u3067\u3001\u5171\u62bd\u51fa\u3055\u308c\u305f\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u306f\u5206\u6790\u6bce\u306b\u77ed\u6642\u9593\u3067\u30ab\u30e9\u30e0\u6d17\u6d44\u304c\u3067\u304d\u306a\u3044\u307b\u3069\u5f37\u304f\u4fdd\u6301\u3055\u308c\u307e\u305b\u3093\u3002\u305d\u306e\u7d50\u679c\u3001 Allure Acrylamide \u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3059\u308b\u30e9\u30dc\u3067\u306f\u3001\u3088\u308a\u5c11\u306a\u3044\u30ab\u30e9\u30e0\u672c\u6570\u3067\u3088\u308a\u591a\u304f\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u5206\u6790\u3067\u304d\u307e\u3059\u3002\u3053\u308c\u306f\u3001\u3042\u3089\u3086\u308b\u98df\u54c1\u5206\u6790\u6a5f\u95a2\u306b\u3068\u3063\u3066\u5f37\u529b\u306a\u30b3\u30b9\u30c8\u7bc0\u7d04\u306e\u7d44\u307f\u5408\u308f\u305b\u3067\u3059\u3002\u6b21\u306e\u4f8b\u306f\u3001\u591a\u691c\u4f53\u3092\u53d6\u308a\u6271\u3046\u98df\u54c1\u691c\u67fb\u6a5f\u95a2\u306b\u304a\u3051\u308b\u3001\u91cd\u6c34\u7d20\u5316\u5185\u90e8\u6a19\u6e96\u7269\u8cea\u3068\u3068\u3082\u306b Allure Acrylamide \u30ab\u30e9\u30e0\u3068\u30ac\u30fc\u30c9\u30ab\u30fc\u30c8\u30ea\u30c3\u30b8\u3092\u4f7f\u7528\u3059\u308b\u5229\u70b9\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u77ed\u3044\u5206\u6790\u6642\u9593<\/h3>\n\n\n\n<p>\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u5316\u5408\u7269\u306f Allure Acrylamide\u30ab\u30e9\u30e0\u304b\u3089\u8fc5\u901f\u306b\u6eb6\u51fa\u3059\u308b\u305f\u3081\u3001\u6700\u9069\u6761\u4ef6\u4e0b\u3067\u904b\u7528\u3057\u305fPGC\u30ab\u30e9\u30e0\u3088\u308a\u3082\u7d20\u65e9\u304f\u5e73\u8861\u5316\u3057\u6b21\u306e\u5206\u6790\u306b\u5099\u3048\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u56f31\u306f\u3001\u98df\u54c1(\u3053\u306e\u30b1\u30fc\u30b9\u3067\u306f\u30dd\u30c6\u30c8\u30c1\u30c3\u30d7\u30b9)\u4e2d\u306e\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u5206\u6790\u306e2\u3064\u306e\u7570\u306a\u308b\u30e1\u30bd\u30c3\u30c9\u306e\u4f8b\u3067\u3059\u30021\u3064\u306fAllure Acrylamide\u30ab\u30e9\u30e0\u3092\u3001\u3082\u30461\u3064\u306f\u4ee3\u8868\u7684\u306aPGC\u30ab\u30e9\u30e0\u3092\u7528\u3044\u3066\u3044\u307e\u3059\u3002PGC\u30ab\u30e9\u30e0\u3092\u7528\u3044\u305f\u30e1\u30bd\u30c3\u30c9\u3067\u306f\u3001EN\u30e1\u30bd\u30c3\u30c9\u304b\u3089\u3055\u3089\u306b\u6700\u9069\u5316\u3057\u3001\u5206\u6790\u6642\u9593\u30928\u5206\u304b\u30897\u5206\u306b\u77ed\u7e2e\u3057\u3064\u3064\u3001\u6700\u9069\u306a\u30ab\u30e9\u30e0\u30d5\u30e9\u30c3\u30b7\u30f3\u30b0\u304a\u3088\u3073\u5e73\u8861\u5316\u6642\u9593\u3092\u7dad\u6301\u3057\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u30d5\u30e9\u30c3\u30b7\u30f3\u30b0\u3057\u3066\u3082\u3001\u30ab\u30e9\u30e0\u306e\u8010\u4e45\u6027\u306b\u5927\u304d\u306a\u5411\u4e0a\u306f\u898b\u3089\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002 Allure Acrylamide\u30ab\u30e9\u30e0\u306b\u3088\u308b\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u5206\u6790\u306f\u3001\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u6210\u5206\u304b\u3089\u826f\u597d\u306a\u5206 \u96e2\u30671.7\u5206\u306e\u4fdd\u6301\u3068\u3044\u3046\u30e1\u30bd\u30c3\u30c9\u8981\u4ef6\u3092\u6e80\u305f\u3057\u306a\u3089\u304c\u3082\u3001\u5e73\u8861\u5316\u6642\u9593\u306f\u306f\u308b\u304b\u306b\u77ed\u304f\u306a\u308a\u307e\u3057\u305f\u3002EN 16618\uff1a2015 \u3068\u6bd4\u8f03\u3057\u30661\u5206\u6790\u3042\u305f\u308a2.5\u5206\u3001\u6700\u9069\u5316\u3057\u305fPGC\u30ab\u30e9\u30e0\u30e1\u30bd\u30c3\u30c9\u3068\u6bd4\u8f03\u3057\u3066\u30821.5\u5206\u306e\u77ed\u7e2e\u3068\u306a\u308b\u305f\u3081\u591a\u304f\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u5206\u6790\u3067\u304d\u3001\u30e9\u30dc\u306e\u751f\u7523\u6027\u304c\u5411\u4e0a\u3057\u307e\u3059\u3002<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>\u56f3 1:<\/strong>\u00a0\u30dd\u30c6\u30c8\u30c1\u30c3\u30d7\u30b9\u306e\u3088\u3046\u306a\u8907\u96d1\u306a\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u306e\u5834\u5408\u3067\u3082\u5e73\u8861\u5316\u306b\u304b\u304b\u308b\u6642\u9593\u304cPGC\u30ab\u30e9\u30e0\u3088\u308a\u306f\u308b\u304b\u306b\u77ed\u3044\u305f\u3081\u3001Allure Acrylamide\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067\u30b5\u30f3\u30d7\u30eb\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u3092\u5897\u52a0\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0532.png\" alt=\"Comparison: Acrylamide Extracted from Potato Chips on an Allure Acrylamide Column vs. a Porous Graphitized Carbon Column\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0532<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor<\/th><th style=\"text-align: center;width: 75px\">Product<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">Acrylamide-d3 (IS)<\/td><td class=\"oth\">200<\/td><td class=\"oth\">75.1<\/td><td class=\"oth\">58.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Acrylamide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/79-06-1\/Acrylamide\" target=\"_blank\" rel=\"noopener\">Acrylamide<\/a><\/td><td class=\"oth\">Endogenous<\/td><td class=\"oth\">72.1<\/td><td class=\"oth\">55.1<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>See notes<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>22 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Acrylamide-d3<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td>A. 0.001% Formic acid in water, B. 0.001% formic acid in acetonitrile<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow:<\/th><td>0.4 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Extracted per EN 16618:2015<br \/><br \/>Weighed 2.0 g of homogenized potato chips into a 50 mL centrifuge tube. Added 40 mL water followed by the addition of internal standard. Shook by hand for 30 sec, by vortexer for 15 sec, and then on a mechanical shaker for 60 min set to maximum sample extraction agitation. Centrifuged in a refrigerated centrifuge at 10 \u00b0C, 3600 x g for 20 min. Removed the aqueous layer after centrifugation, taking care to avoid the top, fatty layer, or the solids at the bottom of the tube. Placed the aqueous extract in an appropriate container.<br \/><br \/>For cleanup, the first SPE cartridge (multimode SPE column with nonpolar, SAX, and SCX properties, 1000 mg\/6 mL) was conditioned with 3 mL methanol and x2, 6 mL aliquots of water. Passed 10 mL of the aqueous extract through the column and collected eluate. For the next cleanup step, the second SPE cartridge (crosslinked polystyrene\/poly-DVB SPE column, 500 mg\/6 mL) was conditioned with 5 mL methanol and 5 mL water. Passed the eluate from the previous step entirely through the column. Rinsed the loaded cartridge once with 4 mL water and discarded the rinsing solvent. Eluted the acrylamide with 2 mL of 60% methanol in water. Collected the sample and transferred into an evaporation tube. Placed the tube in an evaporator at a temperature no higher than 40 \u00b0C to remove the methanol. Evaporated until the final volume was 0.5\u20130.8 mL using a gentle flow of nitrogen. Transferred the final sample into an autosampler vial and analyzed by LC-MS\/MS.<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td><b>Column Details<\/b><br \/>A. <i>Allure Acrylamide column<\/i>: 5 \u03bcm, 50 mm x 2.1 mm ID analytical column (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9167552?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0532\" rel=\"noopener\">cat.# 9167552<\/a>) with 5 \u03bcm, 10 mm x 2.1 mm ID guard cartridge (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/916750212?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0532\" rel=\"noopener\">cat.# 916750212<\/a>).<br \/>B. <i>Porous graphitized carbon column<\/i>: 5 \u03bcm, 50 mm x 2.1 mm ID analytical column with 5 \u03bcm, 10 mm x 2.1 mm ID guard cartridge.<br \/><br \/><b>Mobile Phase Gradients (%B)<\/b><br \/>A. <i>Allure Acrylamide column<\/i>: 0.00 min (0%), 1.00 min (0%), 2.00 min (90%), 2.01 min (0%), 5.50 min (0%), flow = 0.4 mL\/min.<br \/>B. <i>Porous graphitized carbon column<\/i>: 0.00 min (0%), 1.70 min (0%), 2.70 min (90%), 2.71 min (0%), 7.00 min (0%), flow = 0.4 mL\/min.<br \/><\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0532.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">\u512a\u308c\u305f\u8010\u4e45\u6027<\/h3>\n\n\n\n<p>\u56f32\u306f\u3001\u4ee3\u8868\u7684\u306aPGC\u30ab\u30e9\u30e0\u3068\u6bd4\u3079\u3066Allure Acrylamide\u30ab\u30e9\u30e0\u3067\u306f\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u306e\u4fdd\u6301\u6642\u9593\u304c\u975e\u5e38\u306b\u5b89\u5b9a\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002PGC\u30ab\u30e9\u30e0\u3067\u306f\u3001EN 16618\uff1a2015\u306b\u5f93\u3063\u3066\u62bd\u51fa\u3057\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3057\u305f\u30b3\u30fc\u30d2\u30fc\u30b5\u30f3\u30d7\u30eb\u306e\u6ce8\u5165\u3092\u958b\u59cb\u3057\u3066\u3059\u3050\u306b\u4fdd\u6301\u304c\u5f31\u304f\u306a\u308a\u306f\u3058\u3081\u3001475\u56de\u6ce8\u5165\u3059\u308b\u3068\u6700\u7d42\u7684\u306b\u30b7\u30b9\u30c6\u30e0\u9069\u5408\u6027\u306e\u8981\u4ef6\u3067\u3042\u308b1.7\u5206\u306e\u4fdd\u6301\u6642\u9593\u3092\u6e80\u305f\u3059\u3053\u3068\u304c\u3067\u304d\u306a\u304f\u306a\u308a\u307e\u3057\u305f\u3002\u5bfe\u7167\u7684\u306b\u30011000\u56de\u306e\u6ce8\u5165\u5f8c\u3067\u3055\u3048\u3001Allure Acrylamide\u306e\u6027\u80fd\u306f\u5b89\u5b9a\u3057\u3066\u304a\u308a\u3001\u6b21\u306e\u5206\u6790\u306b\u5bfe\u5fdc\u3059\u308b\u6e96\u5099\u304c\u3067\u304d\u3066\u3044\u307e\u3059\u3002\u56f33\u306f\u3001Allure Acrylamide\u30ab\u30e9\u30e0\u3067\u306e\u6700\u521d\u306e\u6ce8\u5165\u304b\u30891000\u56de\u76ee\u306e\u6ce8\u5165\u307e\u3067\u3001\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u304c\u5f71\u97ff\u3092\u53d7\u3051\u306a\u3044\u3053\u3068\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\u5171\u62bd\u51fa\u3055\u308c\u305f\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u5316\u5408\u7269\u3092\u5f37\u304f\u4fdd\u6301\u3059\u308b\u306e\u3067\u306f\u306a\u304f\u6eb6\u51fa\u3067\u304d\u308b\u3053\u3068\u306f\u3001\u30ab\u30e9\u30e0\u30e1\u30f3\u30c6\u30ca\u30f3\u30b9\u3092\u307b\u3068\u3093\u3069\u884c\u308f\u305a\u306b\u3001\u6570\u767e\u56de\u3082\u306e\u7e70\u308a\u8fd4\u3057\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u6ce8\u5165\u5f8c\u3067\u3082\u975e\u5e38\u306b\u5b89\u5b9a\u3057\u305f\u6027\u80fd\u3092\u3082\u305f\u3089\u3059\u30ab\u30ae\u3068\u306a\u308a\u307e\u3059\u3002&nbsp;<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>\u56f3 2:<\/strong>\u00a0Allure Acrylamide\u30ab\u30e9\u30e0\u306f\u30011000\u56de\u6ce8\u5165\u5f8c\u3067\u3082EN 16618\uff1a2015\u30b7\u30b9\u30c6\u30e0\u9069\u5408\u6027\u306e\u8981\u4ef6\u3092\u6e80\u305f\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u306f\u3001\u4e00\u822c\u7684\u306aPGC\u30ab\u30e9\u30e0\u306e2\u500d\u4ee5\u4e0a\u306e\u6ce8\u5165\u56de\u6570\u3067\u3059\u3002<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-FFAR3126A-UNV-02.jpg\" alt=\"decorative\" title=\"-\"><\/figure>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>\u56f3 3:<\/strong>\u00a0\u30b7\u30b9\u30c6\u30e0\u30e1\u30f3\u30c6\u30ca\u30f3\u30b9\u3084\u30ac\u30fc\u30c9\u30ab\u30e9\u30e0\u306e\u4ea4\u63db\u3092\u884c\u308f\u305a\u306b\u30b3\u30fc\u30d2\u30fc\u62bd\u51fa\u7269\u30921000\u56de\u6ce8\u5165\u3057\u305f\u5f8c\u3067\u3082\u3001Allure Acrylamide\u30ab\u30e9\u30e0\u306e\u6027\u80fd\u306f\u5909\u308f\u308a\u307e\u305b\u3093\u3002<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0533.png\" alt=\"Lifetime Test: EN16618:2015 Coffee Extract on Allure Acrylamide (1000th Injection)\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0533<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor<\/th><th style=\"text-align: center;width: 75px\">Product<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">Acrylamide-d3 (IS)<\/td><td class=\"oth\">200<\/td><td class=\"oth\">75.1<\/td><td class=\"oth\">58.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Acrylamide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/79-06-1\/Acrylamide\" target=\"_blank\" rel=\"noopener\">Acrylamide<\/a><\/td><td class=\"oth\">Endogenous<\/td><td class=\"oth\">72.1<\/td><td class=\"oth\">55.1<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Allure Acrylamide  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9167552?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0533\" rel=\"noopener\">cat.# 9167552<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>5 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>60 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Allure Acrylamide 10 mm, 2.1 mm ID, 5 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/916750212?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0533\" rel=\"noopener\">cat.# 916750212<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>22 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.001% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.001% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>100<\/td><td>0<\/td><\/tr><tr><td>1.00<\/td><td>0.4<\/td><td>100<\/td><td>0<\/td><\/tr><tr><td>2.00<\/td><td>0.4<\/td><td>10<\/td><td>90<\/td><\/tr><tr><td>2.01<\/td><td>0.4<\/td><td>100<\/td><td>0<\/td><\/tr><tr><td>5.50<\/td><td>0.4<\/td><td>100<\/td><td>0<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Extracted per EN 16618:2015<br \/><br \/>Weighed 2.0 g of ground coffee into a 50 mL centrifuge tube. Added 5 mL <i>n<\/i>-hexane and 40 mL water followed by the addition of internal standard. Shook by hand for 30 sec, by vortexer for 15 sec, and then on a mechanical shaker for 60 min set to maximum sample extraction agitation. Centrifuged in a refrigerated centrifuge at 10 \u00b0C, 3600 x g for 20 min. Removed the aqueous layer after centrifugation, taking care to avoid the top, hexane layer, or the solids at the bottom of the tube. Placed the aqueous extract in an appropriate container.<br \/><br \/>For cleanup, the first SPE cartridge (multimode SPE column with nonpolar, SAX and SCX properties, 1000 mg\/6 mL) was conditioned with 3 mL methanol and x2, 6 mL aliquots of water. Passed 10 mL of the aqueous extract through the column and collected eluate. For the next cleanup step, the second SPE cartridge (crosslinked polystyrene\/poly-DVB SPE column, 500 mg\/6 mL) was conditioned with 5 mL methanol and 5 mL water. Passed the eluate from the previous step entirely through the column. Rinsed the loaded cartridge once with 4 mL water and discarded the rinsing solvent. Eluted the acrylamide with 2 mL of 60% methanol in water. Collected the sample and transferred into an evaporation tube. Placed the tube in an evaporator at a temperature no higher than 40 \u00b0C to remove the methanol. Evaporated until the final volume was 0.5\u20130.8 mL using a gentle flow of nitrogen. Transferred the final sample into an autosampler vial and analyzed by LC-MS\/MS.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0533.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">\u4e00\u8cab\u3057\u305f\u30ab\u30e9\u30e0\u9593\u306e\u6027\u80fd<\/h3>\n\n\n\n<p>100%\u6c34\u6027\u79fb\u52d5\u76f8\u306b\u8010\u3048\u308b\u3088\u3046\u306b\u958b\u767a\u3055\u308c\u305fRestek Allure Acrylamide\u30ac\u30fc\u30c9\u304a\u3088\u3073\u5206\u96e2\u30ab\u30e9\u30e0\u306f\u3001\u5206\u6790\u6bce\u306e\u3001\u305d\u3057\u3066\u30ab\u30e9\u30e0\u9593\u3067\u3082\u5805\u7262\u306a\u6027\u80fd\u3092\u767a\u63ee\u3057\u307e\u3059\u3002\u53e4\u3044\u30ab\u30e9\u30e0\u304b\u3089\u65b0\u3057\u3044Allure Acrylamide\u30ab\u30e9\u30e0\u3078\u4ea4\u63db\u3059\u308b\u6642\u304c\u6765\u305f\u3068\u304d\u3001\u53b3\u683c\u306a\u88fd\u9020\u624b\u9806\u3068\u54c1\u8cea\u30c6\u30b9\u30c8\u306b\u3088\u3063\u3066\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u5206\u6790\u3067\u540c\u3058\u6027\u80fd\u304c\u5f97\u3089\u308c\u308b\u3053\u3068\u3092\u4fdd\u8a3c\u3057\u307e\u3059\uff08\u56f34\uff09\u3002<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>\u56f3 4:<\/strong>\u00a0\u5805\u7262\u306a\u9006\u76f8Allure Acrylamide LC\u30ab\u30e9\u30e0\u306f\u3001\u30ab\u30e9\u30e0\u9593\u304a\u3088\u3073\u30ed\u30c3\u30c8\u9593\u3067\u518d\u73fe\u6027\u306e\u3042\u308b\u7d50\u679c\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0534.png\" alt=\"Lot-to-Lot Reproducibility of Allure Acrylamide\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0534<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor<\/th><th style=\"text-align: center;width: 75px\">Product<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">Acrylamide-d3 (IS)<\/td><td class=\"oth\">200<\/td><td class=\"oth\">75.1<\/td><td class=\"oth\">58.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Acrylamide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/79-06-1\/Acrylamide\" target=\"_blank\" rel=\"noopener\">Acrylamide<\/a><\/td><td class=\"oth\">200<\/td><td class=\"oth\">72.1<\/td><td class=\"oth\">55.1<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Allure Acrylamide  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9167552?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0534\" rel=\"noopener\">cat.# 9167552<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>5 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>60 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Allure Acrylamide 10 mm, 2.1 mm ID, 5 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/916750212?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0534\" rel=\"noopener\">cat.# 916750212<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>22 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Acrylamide (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/30494?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0534\" rel=\"noopener\">cat.# 30494<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>10 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.001% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.001% Formic acid in acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>100<\/td><td>0<\/td><\/tr><tr><td>1.00<\/td><td>0.4<\/td><td>100<\/td><td>0<\/td><\/tr><tr><td>2.00<\/td><td>0.4<\/td><td>10<\/td><td>90<\/td><\/tr><tr><td>2.01<\/td><td>0.4<\/td><td>100<\/td><td>0<\/td><\/tr><tr><td>5.50<\/td><td>0.4<\/td><td>100<\/td><td>0<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0534.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">\u98df\u54c1\u4e2d\u306e\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u5206\u6790\u306e\u305f\u3081\u306e\u3088\u308a\u826f\u3044\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3<\/h2>\n\n\n\n<p>EN 16618\uff1a2015\u3067\u7528\u3044\u3089\u308c\u3066\u3044\u308bPGC\u30ab\u30e9\u30e0\u306f\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u3092\u5341\u5206\u306b\u4fdd\u6301\u3057\u307e\u3059\u304c\u3001\u5171\u62bd\u51fa\u3057\u305f\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u6210\u5206\u3092\u5f37\u304f\u4fdd\u6301\u3059\u308b\u305f\u3081\u5206\u6790\u6bce\u306b\u9577\u3044\u5e73\u8861\u6642\u9593\u304c\u5fc5\u8981\u3067\u3001\u30ab\u30e9\u30e0\u306e\u8010\u4e45\u6027\u304c\u77ed\u304f\u306a\u308a\u307e\u3059\u3002Allure Acrylamide\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3059\u308b\u3068\u3001\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u306e\u5341\u5206\u306a\u4fdd\u6301\u3092\u78ba\u4fdd\u3057\u306a\u304c\u3089\u3001\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u5316\u5408\u7269\u3092\u3088\u308a\u52b9\u7387\u7684\u304b\u3064\u52b9\u679c\u7684\u306b\u6d17\u6d44\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u305f\u3081\u3001\u5168\u4f53\u7684\u306b\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u5206\u6790\u3092\u8fc5\u901f\u5316\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u305d\u306e\u7d50\u679c\u3001\u30b7\u30b9\u30c6\u30e0\u9069\u5408\u6027\u306e\u8981\u4ef6\u3092\u3088\u308a\u591a\u691c\u4f53\u6ce8\u5165\u306b\u308f\u305f\u308a\u7dad\u6301\u3067\u304d\u308b\u305f\u3081\u3001\u30e1\u30f3\u30c6\u30ca\u30f3\u30b9\u304c\u5fc5\u8981\u306b\u306a\u308b\u524d\u306b\u3088\u308a\u591a\u304f\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u5206\u6790\u3067\u304d\u307e\u3059\u3002Allure Acrylamide\u5206\u96e2\u30ab\u30e9\u30e0\u53ca\u3073\u30ac\u30fc\u30c9\u30ab\u30fc\u30c8\u30ea\u30c3\u30b8\u3068\u91cd\u6c34\u7d20\u5316\u5185\u90e8\u6a19\u6e96\u306e\u7d44\u307f\u5408\u308f\u305b\u306b\u3088\u308a\u3001\u5206\u6790\u30e9\u30dc\u306f\u77ed\u6642\u9593\u304b\u3064\u5c11\u306a\u3044\u30ab\u30e9\u30e0\u672c\u6570\u3067\u3088\u308a\u591a\u304f\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u5206\u6790\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u3001\u751f\u7523\u6027\u3068\u53ce\u76ca\u6027\u306e\u5411\u4e0a\u3078\u3068\u3064\u306a\u304c\u308a\u307e\u3059\u3002&nbsp;<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u53c2\u8003\u6587\u732e<\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li>J.A.G. Roach, D. Andrzejewski, M.L. Gay, D. Nortrup, S.M. Musser, Rugged LC-MS\/MS survey analysis for acrylamide in foods, J. Agric. Food Chem. 51 (2003) 7547\u22127554.&nbsp;<a href=\"https:\/\/pubs.acs.org\/doi\/abs\/10.1021\/jf0346354\" target=\"_blank\" rel=\"noopener\">https:\/\/pubs.acs.org\/doi\/abs\/10.1021\/jf0346354<\/a><\/li>\n<\/ol>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Products Mentioned<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9167552\">                                Catalog No. 9167552                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Allure Acrylamide, 5 \u00b5m, 60 A, 50 x 2.1 mm LC\u30ab\u30e9\u30e0<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9167552\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/916750212\">                                Catalog No. 916750212                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Allure Acrylamide Guard Cartridge, 5 \u00b5m, 60 A, 10 x 2.1 mm, 3-pk.<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/916750212\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/30494\">                                Catalog No. 30494                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Acrylamide Standard, 1000 \u00b5g\/mL, Methanol (dry), 1 mL\/ampul<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/30494\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u98df\u54c1\u4e2d\u306e\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u5206\u6790\u306e\u305f\u3081\u306e\u3053\u306e\u65b9\u6cd5\u306f\u3001\u591a\u5b54\u8cea\u30b0\u30e9\u30d5\u30a1\u30a4\u30c8\u30ab\u30fc\u30dc\u30f3\u30ab\u30e9\u30e0\u306b\u3088\u308b\u30e1\u30bd\u30c3\u30c9\u3068\u6bd4\u8f03\u3057\u3066\u3001\u3088\u308a\u8fc5\u901f\u306a\u5206\u6790\u6642\u9593\u3092\u3082\u305f\u3089\u3057\u3001EN16618.2015\u306e\u30b7\u30b9\u30c6\u30e0\u9069\u5408\u6027\u8981\u4ef6\u3092\u3088\u308a\u9577\u671f\u9593\u6e80\u305f\u3057\u307e\u3059\u3002<\/p>\n","protected":false},"author":11,"featured_media":70536,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[789],"tags":[],"industries-application":[2275,2274],"post-badge":[],"resource-type":[],"product-library":[2571,2579,2554,2553],"resource-technique":[2365,2346,2343],"ppma_author":[414],"class_list":["post-43549","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles-ja","industries-application-food-safety-ja","industries-application-food-beverage-ja","product-library-lc-columns-ja","product-library-liquid-reference-standards-ja","product-library-reference-standard-products-ja","product-library-liquid-chromatography-products-ja","resource-technique-ms-ms-ja","resource-technique-solid-phase-extraction-spe-ja","resource-technique-liquid-chromatography-ja"],"acf":[],"taxonomy_info":{"category":[{"value":789,"label":"\u6280\u8853\u8cc7\u6599"}],"industries-application":[{"value":2275,"label":"\u98df\u306e\u5b89\u5168"},{"value":2274,"label":"\u98df\u54c1\uff5c\u98f2\u6599"}],"product-library":[{"value":2571,"label":"LC \u30ab\u30e9\u30e0"},{"value":2579,"label":"\u6a19\u6e96\u6eb6\u6db2"},{"value":2554,"label":"\u6a19\u6e96\u7269\u8cea"},{"value":2553,"label":"\u6db2\u4f53\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc"}],"resource-technique":[{"value":2365,"label":"MS\/MS"},{"value":2346,"label":"\u56fa\u76f8\u62bd\u51fa (SPE)"},{"value":2343,"label":"\u6db2\u4f53\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-FFAR3126-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/ja\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":789,"name":"\u6280\u8853\u8cc7\u6599","slug":"articles-ja","term_group":0,"term_taxonomy_id":789,"taxonomy":"category","description":"","parent":0,"count":469,"filter":"raw","cat_ID":789,"category_count":469,"category_description":"","cat_name":"\u6280\u8853\u8cc7\u6599","category_nicename":"articles-ja","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/43549","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/comments?post=43549"}],"version-history":[{"count":4,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/43549\/revisions"}],"predecessor-version":[{"id":81366,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/43549\/revisions\/81366"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media\/70536"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media?parent=43549"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/categories?post=43549"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/tags?post=43549"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/industries-application?post=43549"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/post-badge?post=43549"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-type?post=43549"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/product-library?post=43549"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-technique?post=43549"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/ppma_author?post=43549"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}