{"id":43575,"date":"2020-10-21T14:30:00","date_gmt":"2020-10-21T14:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/comprehensive-lc-msms-analysis-of-15-bisphenols-in-8-minutes\/"},"modified":"2025-12-02T19:52:34","modified_gmt":"2025-12-02T19:52:34","slug":"comprehensive-lc-msms-analysis-of-15-bisphenols-in-8-minutes","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/articles\/ffss2935\/comprehensive-lc-msms-analysis-of-15-bisphenols-in-8-minutes","title":{"rendered":"Comprehensive LC-MS\/MS Analysis of 15 Bisphenols in 8 Minutes"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Excellent peak shape and separation for bisphenol A and common analogues.<\/li>\n\n\n\n<li>Simple, no-additives mobile phases and gradient program.<\/li>\n\n\n\n<li>Fast, 8-minute total cycle time.<\/li>\n<\/ul>\n\n\n\n<p>Bisphenol A (BPA), widely used in the production of polycarbonate plastic and epoxy resins, is an endocrine disruptor that imitates naturally occurring hormones or acts as an antagonist, both of which can cause harmful effects on hormone biosynthesis, metabolism, distribution, and mode of action. Hormone disruption can result in detrimental effects to health, growth, and reproduction. Children and unborn babies are most susceptible to hormonal and neurological development problems, and the prevalent use of BPA in many consumer products, including food and beverage packaging, adhesives, and toys, has caused several governments to investigate its safety. Due to the wide range of exposure routes and vulnerability of children, negative public perception has driven many products to advertise as &#8220;BPA-free,&#8221; instead opting to use BPA analogues that have similar physicochemical properties. However, these alternate bisphenols are understudied and may also have harmful toxicological profiles. Some research has shown that these compounds, notably BPF, BPS, BPAF, BPZ, BPE, and BPB, are estrogenic endocrine disruptors and may cause health effects similar to BPA [1,2].<\/p>\n\n\n\n<p>Establishing accurate methods for the analysis of bisphenols that include a broad suite of compounds, rather than just BPA, is imperative for both investigating toxicology and monitoring human exposure. The simple LC-MS\/MS gradient method presented here was developed on a Raptor Biphenyl column because it provides excellent chromatographic peak shape and baseline separation of 15 bisphenols, including BPA and its most prevalent analogues. The 1.8 \u00b5m, 2.1 x 50 column format takes advantage of the inherent speed gains of small particle size technology, resulting in a fast, 8-minute analysis that is ideal for high-throughput testing.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0518.png\" alt=\"Bisphenols on Raptor Biphenyl 1.8 \u03bcm\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0518<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol S\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-09-1\/Bisphenol S\" target=\"_blank\" rel=\"noopener\">Bisphenol S<\/a><\/td><td class=\"oth\">0.84<\/td><td class=\"oth\">5.00<\/td><td class=\"oth\">249.2<\/td><td class=\"oth\">108.1<\/td><td class=\"oth\">92.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol F\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2467-02-9\/Bisphenol F\" target=\"_blank\" rel=\"noopener\">Bisphenol F<\/a><\/td><td class=\"oth\">1.62<\/td><td class=\"oth\">350<\/td><td class=\"oth\">199.3<\/td><td class=\"oth\">93.1<\/td><td class=\"oth\">105.1<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol E\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2081-08-5\/Bisphenol E\" target=\"_blank\" rel=\"noopener\">Bisphenol E<\/a><\/td><td class=\"oth\">2.06<\/td><td class=\"oth\">100<\/td><td class=\"oth\">213.3<\/td><td class=\"oth\">198.3<\/td><td class=\"oth\">197.4<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol A\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/80-05-7\/Bisphenol A\" target=\"_blank\" rel=\"noopener\">Bisphenol A<\/a><\/td><td class=\"oth\">2.50<\/td><td class=\"oth\">100<\/td><td class=\"oth\">227.3<\/td><td class=\"oth\">212.3<\/td><td class=\"oth\">133.1<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol AF\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1478-61-1\/Bisphenol AF\" target=\"_blank\" rel=\"noopener\">Bisphenol AF<\/a><\/td><td class=\"oth\">2.71<\/td><td class=\"oth\">2.00<\/td><td class=\"oth\">335.2<\/td><td class=\"oth\">265.3<\/td><td class=\"oth\">177.3<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol B\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/77-40-7\/Bisphenol B\" target=\"_blank\" rel=\"noopener\">Bisphenol B<\/a><\/td><td class=\"oth\">3.13<\/td><td class=\"oth\">100<\/td><td class=\"oth\">241.3<\/td><td class=\"oth\">212.4<\/td><td class=\"oth\">211.3<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol C\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/79-97-0\/Bisphenol C\" target=\"_blank\" rel=\"noopener\">Bisphenol C<\/a><\/td><td class=\"oth\">3.43<\/td><td class=\"oth\">350<\/td><td class=\"oth\">255.3<\/td><td class=\"oth\">240.4<\/td><td class=\"oth\">147.3<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol AP\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1571-75-1\/Bisphenol AP\" target=\"_blank\" rel=\"noopener\">Bisphenol AP<\/a><\/td><td class=\"oth\">3.98<\/td><td class=\"oth\">25.0<\/td><td class=\"oth\">289.3<\/td><td class=\"oth\">274.3<\/td><td class=\"oth\">273.3<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol Z\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/843-55-0\/Bisphenol Z\" target=\"_blank\" rel=\"noopener\">Bisphenol Z<\/a><\/td><td class=\"oth\">4.25<\/td><td class=\"oth\">250<\/td><td class=\"oth\">267.2<\/td><td class=\"oth\">173.4<\/td><td class=\"oth\">145.2<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol G\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/127-54-8\/Bisphenol G\" target=\"_blank\" rel=\"noopener\">Bisphenol G<\/a><\/td><td class=\"oth\">4.72<\/td><td class=\"oth\">250<\/td><td class=\"oth\">311.2<\/td><td class=\"oth\">295.4<\/td><td class=\"oth\">296.4<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol FL\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/3236-71-3\/Bisphenol FL\" target=\"_blank\" rel=\"noopener\">Bisphenol FL<\/a><\/td><td class=\"oth\">4.90<\/td><td class=\"oth\">50.0<\/td><td class=\"oth\">348.8<\/td><td class=\"oth\">256.2<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol BP\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1844-01-5\/Bisphenol BP\" target=\"_blank\" rel=\"noopener\">Bisphenol BP<\/a><\/td><td class=\"oth\">5.14<\/td><td class=\"oth\">50.0<\/td><td class=\"oth\">351.2<\/td><td class=\"oth\">273.3<\/td><td class=\"oth\">274.3<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol M\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/13595-25-0\/Bisphenol M\" target=\"_blank\" rel=\"noopener\">Bisphenol M<\/a><\/td><td class=\"oth\">5.39<\/td><td class=\"oth\">15.0<\/td><td class=\"oth\">345.2<\/td><td class=\"oth\">330.3<\/td><td class=\"oth\">251.4<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol P\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2167-51-3\/Bisphenol P\" target=\"_blank\" rel=\"noopener\">Bisphenol P<\/a><\/td><td class=\"oth\">5.67<\/td><td class=\"oth\">50.0<\/td><td class=\"oth\">345.2<\/td><td class=\"oth\">330.4<\/td><td class=\"oth\">315.3<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bisphenol PH\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/24038-68-4\/Bisphenol PH\" target=\"_blank\" rel=\"noopener\">Bisphenol PH<\/a><\/td><td class=\"oth\">6.11<\/td><td class=\"oth\">350<\/td><td class=\"oth\">379.2<\/td><td class=\"oth\">209.4<\/td><td class=\"oth\">364.4<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0518\" rel=\"noopener\">cat.# 9309252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>1.8 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>25 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>75:25 Water:methanol<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>2.00-350 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.45<\/td><td>50<\/td><td>50<\/td><\/tr><tr><td>6.50<\/td><td>0.45<\/td><td>10<\/td><td>90<\/td><\/tr><tr><td>6.51<\/td><td>0.45<\/td><td>50<\/td><td>50<\/td><\/tr><tr><td>8.00<\/td><td>0.45<\/td><td>50<\/td><td>50<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0518.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">References<\/h3>\n\n\n\n<p>[1] R. Mesnage, A. Phedonos, M. Arno, S. Balu, J.C. Corton, M.N. Antoniou, Transcriptome profiling reveals bisphenol A alternatives activate estrogen receptor alpha in human breast cancer cells. Toxicol. Sci. 158 (2) (2017) 431-443.&nbsp;<a href=\"https:\/\/doi.org\/10.1093\/toxsci\/kfx101\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/doi.org\/10.1093\/toxsci\/kfx101<\/a><br>[2] R. Barouki, E. Tarroja, C. Persoz, C. Thomsen, E. Heyvaert, H. Reyders, K. Van Campenhout, G. Schoeters, C. Ganzleben, C. Hartmann, M. Uhl, J.-P. Antignac, L. Debrauwer, N. Janey Holcer, N. Cingotti, N. Reineke, Scoping documents: prioritized substance group: bisphenols. HBM4EU (2018) 1-21.<a href=\"https:\/\/www.hbm4eu.eu\/wp-content\/uploads\/2017\/04\/scoping-document-on-bisphenols.pdf\" target=\"_blank\" rel=\"noreferrer noopener\"> https:\/\/www.hbm4eu.eu\/wp-content\/uploads\/2017\/04\/scoping-document-on-bisphenols.pdf<\/a><\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The analysis of bisphenolic compounds that are used as alternatives to bisphenol A (BPA) is essential for monitoring exposure to and evaluating the safety of these emerging industrial chemicals. The LC-MS\/MS method presented here provides excellent chromatographic peak shape and separation for an extensive list of BPA analogues in a fast, 8-minute analysis.<\/p>\n","protected":false},"author":11,"featured_media":70234,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[7],"tags":[],"industries-application":[2236,2238],"post-badge":[],"resource-type":[2623],"product-library":[2499,2481],"resource-technique":[2326,2363],"ppma_author":[414],"class_list":["post-43575","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles","industries-application-lebensmittel-getranke","industries-application-lebensmittelsicherheit","resource-type-featured-application","product-library-lc-saulen","product-library-flussigchromatographie-produkte","resource-technique-flussigchromatografie","resource-technique-ms-ms-de"],"acf":[],"taxonomy_info":{"category":[{"value":7,"label":"Articles"}],"industries-application":[{"value":2236,"label":"Lebensmittel &amp; 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