{"id":43582,"date":"2020-10-21T14:30:00","date_gmt":"2020-10-21T14:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/japanese-5-5-minute-lc-msms-analysis-of-mycotoxins-in-peanut-powder\/"},"modified":"2026-02-19T20:43:54","modified_gmt":"2026-02-19T20:43:54","slug":"japanese-5-5-minute-lc-msms-analysis-of-mycotoxins-in-peanut-powder","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/articles-ja\/ffss2971-ja\/japanese-5-5-minute-lc-msms-analysis-of-mycotoxins-in-peanut-powder","title":{"rendered":"\u5206\u6790\u6642\u95935.5\u5206\u306eLC-MS\/MS\u3092\u7528\u3044\u305f\u30d4\u30fc\u30ca\u30c3\u30c4\u30d1\u30a6\u30c0\u30fc\u4e2d\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3\u5206\u6790"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>\u3088\u308a\u9ad8\u3044\u30b5\u30f3\u30d7\u30eb\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u306e\u305f\u3081\u306e\u9ad8\u901f\u5206\u6790<\/li>\n\n\n\n<li>\u512a\u308c\u305f\u5206\u96e2\u306b\u3088\u308a12\u7a2e\u985e\u306e\u898f\u5236\u5bfe\u8c61\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3\u306e\u7cbe\u5ea6\u304c\u5411\u4e0a<\/li>\n\n\n\n<li>\u8fc5\u901f\u3067\u7c21\u5358\u306a\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u3000(\u5e0c\u91c8-\u308d\u904e-\u6ce8\u5165)<\/li>\n<\/ul>\n\n\n\n<p>\u8fb2\u7523\u7269\u3067\u7e41\u6b96\u3059\u308b\u7279\u5b9a\u306e\u771f\u83cc\u306f\u3001\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3\u3068\u3057\u3066\u77e5\u3089\u308c\u308b\u6709\u6bd2\u306a\u4ee3\u8b1d\u7269\u3092\u7523\u751f\u3057\u307e\u3059\u3002\u73fe\u4ee3\u306e\u98df\u54c1\u52a0\u5de5\u5de5\u7a0b\u3067\u306f\u3053\u308c\u3089\u306e\u5316\u5408\u7269\u304c\u5b58\u5728\u3057\u3066\u3082\u5b8c\u5168\u306b\u9664\u53bb\u3067\u304d\u306a\u3044\u305f\u3081\u3001\u53b3\u3057\u3044\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u624b\u6cd5\u304c\u78ba\u7acb\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3\u5206\u6790\u306e\u6c4e\u7528\u6cd5\u304c\u958b\u767a\u3055\u308c\u308b\u969b\u306b\u306f\u975e\u5e38\u306b\u52b9\u7387\u7684\u306a\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u304c\u63a1\u7528\u3055\u308c\u308b\u3053\u3068\u306b\u306a\u308b\u3067\u3057\u3087\u3046\u3002\u3057\u304b\u3057\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3\u306e\u7269\u7406\u5316\u5b66\u7684\u6027\u8cea\u306e\u9055\u3044\u3084\u3001\u62bd\u51fa\u52b9\u7387\u3001\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u52b9\u679c\u306b\u3088\u308a\u3053\u306e\u3088\u3046\u306a\u30e1\u30bd\u30c3\u30c9\u3092\u958b\u767a\u3059\u308b\u3053\u3068\u306f\u975e\u5e38\u306b\u56f0\u96e3\u3067\u3059\u3002Dr. Zhang\u3089\u306f\u591a\u69d8\u306a\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u4e2d\u306e\u69d8\u3005\u306a\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3\u306e\u5206\u6790\u306b\u5e83\u304f\u9069\u7528\u3067\u304d\u308b\u5206\u6790\u624b\u9806\u3092\u63d0\u4f9b\u3059\u308b\u3053\u3068\u3092\u76ee\u7684\u3068\u3057\u3066\u3001\u8907\u6570\u6a5f\u95a2\u306b\u304a\u3051\u308b\u5171\u540c\u7814\u7a76[1]\u3092\u516c\u958b\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u7814\u7a76\u306b\u30d2\u30f3\u30c8\u3092\u5f97\u3066\u3001\u5f93\u6765\u578bHPLC\u6a5f\u5668\u306e\u8010\u5727\u7bc4\u56f2\u3067FDA\u306e\u898f\u5236\u5bfe\u8c61\u3067\u3042\u308b12\u7a2e\u306e\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3\u3092\u5206\u96e2\u3059\u308b\u3001\u5f8c\u8ff0\u306eLC-MS\/MS\u6cd5\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<p>\u3053\u306e\u4f8b\u3067\u306f\u3001\u30d4\u30fc\u30ca\u30c3\u30c4\u30d1\u30a6\u30c0\u30fc\u3092\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u3068\u3057\u3066\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3\u3092\u5206\u6790\u3057\u307e\u3057\u305f\u3002\u6bd4\u8f03\u7684\u77ed\u3044\u30ab\u30e9\u30e0\u3001Biphenyl\u56fa\u5b9a\u76f8\u306e\u9078\u629e\u6027\u3001\u52b9\u7387\u7684\u306a2.7\u03bcm\u306eRaptor\u8868\u9762\u591a\u5b54\u8cea\uff08\u30b3\u30a2\u30b7\u30a7\u30eb\uff09\u7c92\u5b50\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u30675.5\u5206\uff081\u30b5\u30a4\u30af\u30eb\u3067\u306f\u30c8\u30fc\u30bf\u30eb7\u5206\uff09\u3068\u3044\u3046\u8fc5\u901f\u306a\u5206\u6790\u6642\u9593\u3067\u512a\u308c\u305f\u5206\u96e2\u304c\u5f97\u3089\u308c\u307e\u3057\u305f\u3002\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3HT-2\u306e\u611f\u5ea6\u306e\u826f\u3044MRM\u30c8\u30e9\u30f3\u30b8\u30b7\u30e7\u30f3447.3-285.3\u306b\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u7531\u6765\u5316\u5408\u7269\u306e\u5171\u6eb6\u51fa\u304c\u89b3\u5bdf\u3055\u308c\u305f\u305f\u3081\u3001\u5b9a\u91cf\u306b\u306f447.3-345.3\u3092\u9078\u629e\u3057\u307e\u3057\u305f\u3002\u611f\u5ea6\u3092\u5411\u4e0a\u3055\u305b\u308b\u305f\u3081\u306b\u3001\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3\u306e\u30a4\u30aa\u30f3\u5316\u3092\u4fc3\u9032\u3059\u308b\u30a2\u30f3\u30e2\u30cb\u30a6\u30e0\u7de9\u885d\u6db2\u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002\u53c2\u8003\u6587\u732e\u3067\u7814\u7a76\u3055\u308c\u305f12\u7a2e\u306e\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3\u306b\u304a\u3044\u3066Raptor Bipheny\u30ab\u30e9\u30e0\u306f\u975e\u5e38\u306b\u6709\u52b9\u3067\u3057\u305f\u304c\u3001\u985e\u4f3c\u306e\u69cb\u9020\u3092\u6301\u3064\u30a2\u30a4\u30bd\u30d0\u30ea\u30c3\u30af\u306a\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3\u3092\u542b\u3080\u3001\u3055\u3089\u306b\u591a\u6570\u306e\u5316\u5408\u7269\u7fa4\u306b\u304a\u3044\u3066\u306f\u3001\u5145\u5206\u306a\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u5206\u96e2\u306bRaptor FluoroPhenyl\u56fa\u5b9a\u76f8\u304c\u5fc5\u8981\u3068\u306a\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u3053\u306e\u30e1\u30bd\u30c3\u30c9\u306f\u3001\u69d8\u3005\u306a\u30de\u30c8\u30ea\u30c3\u30af\u30b9\uff08\u3053\u3053\u3067\u793a\u3057\u305f\u30d4\u30fc\u30ca\u30c3\u30c4\u30d1\u30a6\u30c0\u30fc\u306b\u52a0\u3048\u3066\u30b3\u30fc\u30f3\u30df\u30fc\u30eb\u3084\u7384\u7c73\u7c89\u3068\u3044\u3063\u305f\u8907\u6570\u306e\u7531\u6765\u3092\u542b\u3080\uff09\u3092\u5bfe\u8c61\u3068\u3057\u305f\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u7814\u7a76\u3067\u8a55\u4fa1\u3055\u308c\u305f\u3001FDA\u306e\u898f\u5236\u5bfe\u8c61\u3067\u3042\u308b12\u7a2e\u306e\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3\u306b\u5bfe\u3057\u3066\u3001\u512a\u308c\u305f\u7cbe\u5ea6\u3068\u7cbe\u78ba\u3055\u3092\u793a\u3057\u307e\u3057\u305f\u3002Restek\u306f\u3053\u306e\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u3092\u901a\u3057\u3066\u6280\u8853\u30b5\u30dd\u30fc\u30c8\u3057\u3066\u3044\u305f\u3060\u3044\u305fDr. Zhang\u306b\u611f\u8b1d\u7533\u3057\u4e0a\u3052\u307e\u3059\u3002<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0526.png\" alt=\"Mycotoxins in Peanut Powder on Raptor Biphenyl by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0526<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/g)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Deoxynivalenol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51481-10-8\/Deoxynivalenol\" target=\"_blank\" rel=\"noopener\">Deoxynivalenol<\/a><\/td><td class=\"oth\">0.62<\/td><td class=\"oth\">50<\/td><td class=\"oth\">297.3<\/td><td class=\"oth\">249.3<\/td><td class=\"oth\">231.2<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">Fumonisin B1<\/td><td class=\"oth\">2.45<\/td><td class=\"oth\">50<\/td><td class=\"oth\">722.5<\/td><td class=\"oth\">352.4<\/td><td class=\"oth\">334.5<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for HT-2\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/26934-87-2\/HT-2\" target=\"_blank\" rel=\"noopener\">HT-2<\/a><\/td><td class=\"oth\">2.60<\/td><td class=\"oth\">50<\/td><td class=\"oth\">447.3<\/td><td class=\"oth\">345.3<\/td><td class=\"oth\">285.3<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\">Fumonisin B3<\/td><td class=\"oth\">2.85<\/td><td class=\"oth\">50<\/td><td class=\"oth\">706.5<\/td><td class=\"oth\">336.4<\/td><td class=\"oth\">318.4<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\">Fumonisin B2<\/td><td class=\"oth\">3.23<\/td><td class=\"oth\">50<\/td><td class=\"oth\">706.5<\/td><td class=\"oth\">336.3<\/td><td class=\"oth\">141.2<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for T2\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-31-7\/T2\" target=\"_blank\" rel=\"noopener\">T2<\/a><\/td><td class=\"oth\">3.31<\/td><td class=\"oth\">50<\/td><td class=\"oth\">489.3<\/td><td class=\"oth\">245.2<\/td><td class=\"oth\">387.4<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/g)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aflatoxin G2\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/7241-98-7\/Aflatoxin G2\" target=\"_blank\" rel=\"noopener\">Aflatoxin G2<\/a><\/td><td class=\"oth\">3.74<\/td><td class=\"oth\">5<\/td><td class=\"oth\">331.2<\/td><td class=\"oth\">313.3<\/td><td class=\"oth\">189.3<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Zearalenone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/17924-92-4\/Zearalenone\" target=\"_blank\" rel=\"noopener\">Zearalenone<\/a><\/td><td class=\"oth\">3.96<\/td><td class=\"oth\">50<\/td><td class=\"oth\">319.3<\/td><td class=\"oth\">283.3<\/td><td class=\"oth\">187.2<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aflatoxin G1\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1165-39-5\/Aflatoxin G1\" target=\"_blank\" rel=\"noopener\">Aflatoxin G1<\/a><\/td><td class=\"oth\">4.22<\/td><td class=\"oth\">5<\/td><td class=\"oth\">329.2<\/td><td class=\"oth\">243.2<\/td><td class=\"oth\">200.2<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aflatoxin B2\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/7220-81-7\/Aflatoxin B2\" target=\"_blank\" rel=\"noopener\">Aflatoxin B2<\/a><\/td><td class=\"oth\">4.43<\/td><td class=\"oth\">5<\/td><td class=\"oth\">315.3<\/td><td class=\"oth\">287.3<\/td><td class=\"oth\">259.2<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aflatoxin B1\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1162-65-8\/Aflatoxin B1\" target=\"_blank\" rel=\"noopener\">Aflatoxin B1<\/a><\/td><td class=\"oth\">4.99<\/td><td class=\"oth\">5<\/td><td class=\"oth\">313.3<\/td><td class=\"oth\">285.2<\/td><td class=\"oth\">241.2<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ochratoxin A\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/303-47-9\/Ochratoxin A\" target=\"_blank\" rel=\"noopener\">Ochratoxin A<\/a><\/td><td class=\"oth\">5.19<\/td><td class=\"oth\">5<\/td><td class=\"oth\">404.2<\/td><td class=\"oth\">239.3<\/td><td class=\"oth\">358.3<\/td><\/tr>\n<\/tbody><\/table><div class=\"peaknotes\">Matrix interference was observed for HT-2 transition 447.3-285.3 in peanut powder. Therefore, 447.3-345.3 was chose for quantification.<\/div><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0526\" rel=\"noopener\">cat.# 9309A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0526\" rel=\"noopener\">cat.# 9309A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 2 mM ammonium formate, 0.1% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol, 2 mM ammonium formate, 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><th>%C<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>70<\/td><td>30<\/td><td><\/td><\/tr><tr><td>0.6<\/td><td>0.5<\/td><td>70<\/td><td>30<\/td><td><\/td><\/tr><tr><td>0.7<\/td><td>0.5<\/td><td>50<\/td><td>50<\/td><td><\/td><\/tr><tr><td>3.0<\/td><td>0.5<\/td><td>25<\/td><td>70<\/td><td><\/td><\/tr><tr><td>4.5<\/td><td>0.5<\/td><td>25<\/td><td>75<\/td><td><\/td><\/tr><tr><td>5.0<\/td><td>0.5<\/td><td>10<\/td><td>90<\/td><td><\/td><\/tr><tr><td>5.2<\/td><td>0.5<\/td><td>10<\/td><td>90<\/td><td><\/td><\/tr><tr><td>5.21<\/td><td>0.5<\/td><td>25<\/td><td>75<\/td><td><\/td><\/tr><tr><td>6.00<\/td><td>0.5<\/td><td>25<\/td><td>75<\/td><td><\/td><\/tr><tr><td>6.01<\/td><td>0.5<\/td><td>70<\/td><td>30<\/td><td><\/td><\/tr><tr><td>7.00<\/td><td>0.5<\/td><td>70<\/td><td>30<\/td><td> <\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Weighed 1.00 gram of peanut powder in a 50 mL centrifuge tube and added 2.00 mL of water. Vortexed at 3000 rpm for 5 min followed by the addition of 4.0 mL of extraction solvent (50:50 water:acetonitrile, v\/v). The tube was then vortexed at 3000 rpm for 5 min followed by centrifugation for 15 min at 4200 rpm. 475 \u03bcL of the supernatant was filtered through a Thomson SINGLE StEP Nano filter vial (0.2 \u03bcm). The sample was then fortified with 25 \u03bcL of a standard solution prepared in water at 1000 ng\/mL (100 ng\/mL for aflatoxins and ochratoxin A) as part of the matrix-matched calibration curve. Vortexed at 3000 rpm for 1 min prior to analysis.<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Want even better performance when analyzing metal-sensitive compounds? Check out Inert LC columns at <a href=\"https:\/\/www.restek.com\/inert\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a>. <\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-article-inner\">\n\n<p><\/p>\n\n<\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0526.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><strong>\u53c2\u8003\u6587\u732e<\/strong><\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>K. Zhang, M.R. Schaab, G. Southwood, E.R. Tor, L.S. Aston, W. Song, B. Eitzer, S. Majumdar, T. Lapainis, H. Mai, K. Tran, A. El-Demerdash, V. Vega, Y. Cai, J.W. Wong, A.J. Krynitsky, T.H. Begley, A collaborative study: determination of mycotoxins in corn, peanut butter, and wheat flour using stable isotope dilution assay (SIDA) and liquid chromatography-tandem mass spectrometry (LC-MS\/MS), Journal of Agricultural and Food Chemistry, 65 (33) (2017) 7138-7152.&nbsp;<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27983809\" target=\"_blank\" rel=\"noopener\">https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/27983809<\/a>.<\/li>\n<\/ol>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Products Mentioned<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9309A52\">                                Catalog No. 9309A52                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor Biphenyl, 2.7 \u00b5m, 50 x 2.1 mm HPLC\u30ab\u30e9\u30e0<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9309A52\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9309A0252\">                                Catalog No. 9309A0252                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor Biphenyl EXP Guard Column Cartridge, 2.7 \u00b5m, 5 x 2.1 mm, 3-pk.<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9309A0252\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u3053\u3053\u3067\u7d39\u4ecb\u3059\u308b\u30e1\u30bd\u30c3\u30c9\u306f\u3001\u30d4\u30fc\u30ca\u30c3\u30c4\u30d1\u30a6\u30c0\u30fc\u4e2d\u306e\u30de\u30a4\u30b3\u30c8\u30ad\u30b7\u30f3\u306e\u9ad8\u901f\u5206\u6790\u3092\u53ef\u80fd\u306b\u3057\u307e\u3059\u3002 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