{"id":43587,"date":"2020-10-23T01:00:00","date_gmt":"2020-10-23T01:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/%e9%a3%9f%e7%94%a8%e6%b2%b9%e4%b8%ad%e3%81%ae3-mcpd%e3%81%a8%e3%82%b0%e3%83%aa%e3%82%b7%e3%82%b8%e3%83%ab%e3%82%a8%e3%82%b9%e3%83%86%e3%83%ab%e3%81%ae%e9%ab%98%e9%80%9fgc-ms%e5%88%86%e6%9e%90\/"},"modified":"2025-12-02T22:55:44","modified_gmt":"2025-12-02T22:55:44","slug":"faster-gc-ms-analysis-of-3-mcpd-and-glycidyl-esters-in-edible-oils-ja","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/articles-ja\/ffss3027-ja\/faster-gc-ms-analysis-of-3-mcpd-and-glycidyl-esters-in-edible-oils-ja","title":{"rendered":"\u98df\u7528\u6cb9\u4e2d\u306e3-MCPD\u3068\u30b0\u30ea\u30b7\u30b8\u30eb\u30a8\u30b9\u30c6\u30eb\u306e\u9ad8\u901fGC-MS\u5206\u6790"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>\u6700\u9069\u5316\u3055\u308c\u305fGC\u6761\u4ef6\u3067AOCS Cd 29c-13\u30e1\u30bd\u30c3\u30c9\u306e\u8981\u4ef6\u3092\u6e80\u305f\u3057\u307e\u3059\u3002<\/li>\n\n\n\n<li>\u30b9\u30d7\u30ea\u30c3\u30c8\u6ce8\u5165\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067\u3001\u5206\u6790\u6642\u9593\u30928\u5206\u77ed\u7e2e\u3057\u3001\u8a98\u5c0e\u4f53\u5316\u8a66\u85ac\u306b\u3088\u308b\u30c0\u30e1\u30fc\u30b8\u3092\u8efd\u6e1b\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059<\/li>\n\n\n\n<li>PTV\u3082\u3057\u304f\u306f\u30b9\u30d7\u30ea\u30c3\u30c8\/\u30b9\u30d7\u30ea\u30c3\u30c8\u30ec\u30b9\u6ce8\u5165\u53e3\u3092\u4f7f\u7528\u3067\u304d\u308b\u30d5\u30ec\u30ad\u30b7\u30d6\u30eb\u306a\u5206\u6790\u6cd5\u3067\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<p>\u98df\u7528\u6cb9\u4e2d\u306e3-\u30e2\u30ce\u30af\u30ed\u30ed\u30d7\u30ed\u30d1\u30f3-1,2-\u30b8\u30aa\u30fc\u30eb(3-MCPD)\u8102\u80aa\u9178\u30a8\u30b9\u30c6\u30eb\u3068\u30b0\u30ea\u30b7\u30b8\u30eb\u30a8\u30b9\u30c6\u30eb\u306f\u3001\u7cbe\u88fd\u904e\u7a0b\u306b\u304a\u3051\u308b\u6c5a\u67d3\u7269\u8cea\u3067\u3059\u3002\u6442\u53d6\u3059 \u308b\u3068\u3001\u3053\u308c\u3089\u306e\u5316\u5408\u7269\u306f\u767a\u304c\u3093\u6027\u3082\u3057\u304f\u306f\u907a\u4f1d\u6bd2\u6027\u306e\u53ef\u80fd\u6027\u3092\u6709\u3059\u308b\u904a\u96e2\u306eMCPD\u3068\u30b0\u30ea\u30b7\u30c9\u30fc\u30eb\u3092\u653e\u51fa\u3057\u307e\u3059\u3002\u6700\u3082\u4e00\u822c\u7684\u306a\u5206\u6790\u6cd5\u306f\u3001\u8a66 \u6599\u4e2d\u306e\u30a8\u30b9\u30c6\u30eb\u3092\u52a0\u6c34\u5206\u89e3\u3057\u3001\u8a98\u5c0e\u4f53\u5316\u5f8c\u306bGC-MS\u3067\u5206\u6790\u3059\u308b\u9593\u63a5\u7684\u306a\u3082\u306e\u3067\u3059\u3002AOCS Cd 29c-13\u3084\u305d\u306e\u56fd\u969b\u898f\u683c(ISO 18363-1)\u3082\u3057\u304f\u306f\u30c9 \u30a4\u30c4\u6cb9\u5316\u5b66\u4f1a\u6cd5 (DGF C-VI 18 (10))\u306e\u3088\u3046\u306a\u5206\u6790\u6cd5\u304c\u901a\u5e38\u4f7f\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u904e\u7a0b\u306b\u304a\u3044\u3066\u3001\u9178\u6027\u6761\u4ef6\u4e0b\u3067\u904a\u96e2\u30b0\u30ea\u30b7\u30c9\u30fc\u30eb \u306f3-MCPD\u3078\u3068\u5909\u63db\u3055\u308c\u30013-MCPD\u30a8\u30b9\u30c6\u30eb\u304b\u3089\u904a\u96e2\u3057\u305f3-MCPD\u3068\u4e00\u7dd2\u306b\u6e2c\u5b9a\u3055\u308c\u307e\u3059\u3002\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u6761\u4ef6\u3068\u3057\u3066\u306f\u3001PTV\u6ce8\u5165\u53e3\u3092\u30b9\u30d7\u30ea \u30c3\u30c8\u30ec\u30b9\u30e2\u30fc\u30c9\u3067\u4f7f\u7528\u3059\u308b\u5fc5\u8981\u304c\u751f\u3058\u3001\u30aa\u30fc\u30d6\u30f3\u521d\u671f\u6e29\u5ea6\u309285\u2103\u306b\u3057\u3001\u6eb6\u51fa\u306e\u65e9\u3044\u6210\u5206\u3092\u30b7\u30e3\u30fc\u30d7\u3067\u5bfe\u79f0\u306a\u30d4\u30fc\u30af\u306b\u3059\u308b\u6eb6\u5a92\u30d5\u30a9\u30fc\u30ab\u30b7\u30f3 \u30b0\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3002AOCS Cd 29c-13\u3067\u793a\u3055\u308c\u3066\u3044\u308b\u3088\u3046\u306a\u6607\u6e29\u30d7\u30ed\u30b0\u30e9\u30e0\u3092\u304a\u3053\u306a\u3046\u3068\u3001\u6700\u7d42\u7684\u306a\u5206\u6790\u6642\u9593\u306f24.8\u5206\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u305d\u3053\u3067\u30013-MCPD\u3068\u30b0\u30ea\u30b7\u30b8\u30eb\u30a8\u30b9\u30c6\u30eb\u306e\u5206\u6790\u6642\u9593\u3092\u77ed\u304f\u3059\u308b\u305f\u3081\u306b\u3001AOCS Cd 29c-13\u306e\u8a66\u6599\u5c0e\u5165\u6cd5\u3068\u30aa\u30fc\u30d6\u30f3\u30d7\u30ed\u30b0\u30e9\u30e0\u306e\u6700\u9069\u5316\u3092\u304a\u3053 \u306a\u3044\u307e\u3057\u305f\u3002\u307e\u305a\u3001\u30b9\u30d7\u30ea\u30c3\u30c8\u6ce8\u5165\u306b\u5909\u66f4\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u306b\u3088\u308a\u8a98\u5c0e\u4f53\u5316\u8a66\u85ac\u304c\u30b7\u30b9\u30c6\u30e0\u3078\u5165\u308b\u91cf\u304c\u6e1b\u308b\u305f\u3081\u3001\u30ab\u30e9\u30e0\u3068MS\u306b\u512a\u3057\u304f\u306a\u308a\u3001\u30e1\u30f3 \u30c6\u30ca\u30f3\u30b9\u983b\u5ea6\u3084\u4ea4\u63db\u983b\u5ea6\u3092\u4e0b\u3052\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u3001\u30b9\u30d7\u30ea\u30c3\u30c8\u30ec\u30b9\u306b\u6bd4\u3079\u3066\u3001\u6ce8\u5165\u53e3\u304b\u3089\u30ab\u30e9\u30e0\u3078\u306e\u8a66\u6599\u306e\u79fb\u52d5\u304c\u901f\u304f\u306a\u308a\u3001\u6eb6\u5a92\u30d5\u30a9\u30fc \u30ab\u30b7\u30f3\u30b0\u306f\u4e0d\u8981\u306a\u305f\u3081\u3001\u30aa\u30fc\u30d6\u30f3\u521d\u671f\u6e29\u5ea6\u3092\u4e0a\u3052\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u30aa\u30fc\u30d6\u30f3\u521d\u671f\u6e29\u5ea6\u3092120\u2103\u306b\u4e0a\u3052\u308b\u3068\u3001\u30d4\u30fc\u30af\u306f\u30b7\u30e3\u30fc\u30d7\u306b\u306a\u308a\u3001\u30b9\u30d7 \u30ea\u30c3\u30c8\u6bd410:1\u3067\u3082\u5fc5\u8981\u306a\u691c\u51fa\u9650\u754c\u3092\u9054\u6210\u3067\u304d\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u6607\u6e29\u901f\u5ea6\u30926\u2103\/\u5206\u304b\u308912\u2103\/\u5206\u3078\u5909\u66f4\u3059\u308b\u3068\u3001\u306a\u3093\u306e\u5f0a\u5bb3\u3082\u306a\u304f\u5206\u6790\u6642\u9593\u306e\u77ed\u7e2e \u304c\u3067\u304d\u307e\u3059\u3002\u30aa\u30fc\u30d6\u30f3\u306e\u6700\u7d42\u6e29\u5ea6\u3092\u9ad8\u304f\u3059\u308b\u3053\u3068\u306f\u3001\u30ab\u30e9\u30e0\u304b\u3089\u9ad8\u5206\u5b50\u91cf\u306e\u6c5a\u67d3\u7269\u8cea\u3092\u9664\u53bb\u3059\u308b\u306e\u306b\u52b9\u679c\u7684\u3067\u3059\u3002\u3053\u3053\u3067\u793a\u3057\u305f\u6700\u9069\u5316\u6761\u4ef6 \u306f\u3001AOCS Cd 29c-13\u306e\u5206\u6790\u6642\u9593\u30928\u5206\u3082\u77ed\u7e2e\u3057\u3001PTV\u3068\u5c02\u7528\u30b9\u30d7\u30ea\u30c3\u30c8\/\u30b9\u30d7\u30ea\u30c3\u30c8\u30ec\u30b9\u6ce8\u5165\u53e3\u306e\u4e21\u65b9\u3067\u5b9f\u884c\u3067\u304d\u3001\u3044\u305a\u308c\u306e\u5834\u5408\u3067\u3082\u5206\u6790\u6642\u9593 \u306e\u77ed\u7e2e\u3068\u98df\u7528\u6cb9\u4e2d\u306e3-MCPD\u3068\u30b0\u30ea\u30b7\u30b8\u30eb\u30a8\u30b9\u30c6\u30eb\u306e\u5206\u6790\u6761\u4ef6\u306e\u67d4\u8edf\u6027\u3092\u5b9f\u73fe\u3057\u307e\u3059\u3002<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/gc_fs0566.png\" alt=\"3-Monochloropropane-1,2-Diol (3-MCPD) Esters in Olive Oil on Rxi-17Sil MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> GC_FS0566<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">3-MCPD-d5 PBA derivative<\/td><td class=\"oth\">5.937<\/td><td class=\"oth\">1.81<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">3-MCPD PBA derivative<\/td><td class=\"oth\">5.977<\/td><td class=\"oth\">2.94<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Rxi-17Sil MS, 30 m, 0.25 mm ID, 0.25 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/14123?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0566\" rel=\"noopener\">cat.# 14123<\/a>)<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Extracted olive oil<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Isooctane<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Injection<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1 \u00b5L split (split ratio 10:1)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Liner:<\/th><td>Topaz 4.0 mm ID Precision inlet liner w\/wool (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/23305?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_FS0566\" rel=\"noopener\">cat.# 23305<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Temp.:<\/th><td>280 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\" colspan=\"2\">Oven<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Oven Temp.:<\/th><td>120 &#176;C (hold 0.5 min) to 180 &#176;C at 12 &#176;C\/min to 330 &#176;C at 25 &#176;C\/min (hold 5 min)<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Carrier Gas<\/th><td>He, constant flow<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow Rate:<\/th><td>1.4 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>SIM<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">SIM Program:<\/th><td colspan=\"2\">147, 150, 196, 201 m\/z, 50 ms dwell<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Transfer Line Temp.:<\/th><td>320 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Analyzer Type:<\/th><td>Quadrupole <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Source Temp.:<\/th><td>230 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Quad Temp.:<\/th><td>150 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Agilent 7890A GC &amp; 5975C MSD<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>The olive oil sample was spiked with 3-MCPD-d5 ester and prepared according to AOCS method Cd 29c-13.<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Restek MCPD reference standards are now available. Search for MCPD on www.restek.com to view our full selection.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/gc_fs0566.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Products Mentioned<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/14123\">                                Catalog No. 14123                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Rxi-17Sil MS GC Capillary Column, 30 m, 0.25 mm ID, 0.25 \u00b5m<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/14123\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/23305\">                                Catalog No. 23305                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Topaz, Precision Inlet Liner, 4.0 mm x 6.3 x 78.5, for Agilent GCs, w\/Quartz Wool, Premium Deactivation, 5-pk.<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/23305\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The analysis of 3-MCPD and glycidyl esters using AOCS Cd 29c-13 is time consuming and exposes the GC-MS to harsh derivatization reagents that can limit column lifetime and increase maintenance costs. 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