{"id":43607,"date":"2021-01-12T00:00:00","date_gmt":"2021-01-12T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/japanese-fast-and-rugged-direct-analysis-of-polar-pesticides-in-spinach\/"},"modified":"2026-01-14T19:10:42","modified_gmt":"2026-01-14T19:10:42","slug":"japanese-fast-and-rugged-direct-analysis-of-polar-pesticides-in-spinach","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/articles-ja\/fsfa3215-ja\/japanese-fast-and-rugged-direct-analysis-of-polar-pesticides-in-spinach","title":{"rendered":"\u30db\u30a6\u30ec\u30f3\u30bd\u30a6\u4e2d\u306e\u6975\u6027\u8fb2\u85ac\u306e\u8fc5\u901f\u304b\u3064\u9811\u5065\u306a\u76f4\u63a5\u5206\u6790\u6cd5"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>\u6642\u9593\u306e\u304b\u304b\u308b\u8a98\u5c0e\u4f53\u5316\u3084\u30a4\u30aa\u30f3\u5bfe\u5f62\u6210\u304c\u4e0d\u8981\u3067\u3001\u30ab\u30e9\u30e0\u5e73\u8861\u5316\u3092\u9577\u304f\u5f85\u3064\u3053\u3068\u3082\u3042\u308a\u307e\u305b\u3093\u3002<\/li>\n\n\n\n<li>\u72ec\u81ea\u306e\u56fa\u5b9a\u76f8\u306f\u826f\u597d\u306a\u4fdd\u6301\u3068\u52b9\u7387\u7684\u306a\u6eb6\u51fa\u3092\u3082\u305f\u3089\u3057\u3001\u3088\u308a\u826f\u3044\u30d4\u30fc\u30af\u5f62\u72b6\u3092\u5b9f\u73fe\u3057\u307e\u3059\u3002<\/li>\n\n\n\n<li>\u9811\u5065\u306a\u30ab\u30e9\u30e0\u6027\u80fd\u306b\u3088\u308a\u3001\u4e00\u8cab\u3057\u305f\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3068\u4fe1\u983c\u3067\u304d\u308b\u7d50\u679c\u304c\u78ba\u4fdd\u3055\u308c\u307e\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<p>\u6975\u6027\u8fb2\u85ac\u3001\u4f8b\u3048\u3070\u30b0\u30ea\u30db\u30b5\u30fc\u30c8\u3068\u3001\u305d\u306e\u4e3b\u306a\u4ee3\u8b1d\u7523\u7269AMPA\u3001\u304a\u3088\u3073\u30b0\u30eb\u30db\u30b7\u30cd\u30fc\u30c8\u306e\u76f4\u63a5\u5206\u6790\u306f\u3001\u305d\u308c\u3089\u304c\u9006\u76f8\u30ab\u30e9\u30e0\u4e0a\u3067\u5341\u5206\u306b\u4fdd\u6301\u3055\u308c\u305a\u3001LC\u2010MS\/MS\u306e\u6d41\u8def\u4e2d\u306e\u91d1\u5c5e\u8868\u9762\u3068\u306e\u30ad\u30ec\u30fc\u30c8\u5316\u306b\u3088\u308a\u30ec\u30b9\u30dd\u30f3\u30b9\u304c\u8457\u3057\u304f\u4f4e\u4e0b\u3059\u308b\u305f\u3081\u56f0\u96e3\u3067\u3059\u3002\u8a98\u5c0e\u4f53\u5316\u8a66\u85ac\u3084\u30a4\u30aa\u30f3\u5bfe\u8a66\u85ac\u306f\u6027\u80fd\u306e\u5411\u4e0a\u306e\u305f\u3081\u306b\u4f7f\u7528\u3055\u308c\u308b\u3053\u3068\u304c\u591a\u304f\u3042\u308a\u307e\u3059\u304c\u3001\u8ffd\u52a0\u306e\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u6642\u9593\u3092\u5fc5\u8981\u3068\u3057\u3001\u597d\u307e\u3057\u304f\u306a\u3044\u526f\u4f5c\u7528\uff08\u6c88\u6bbf\u7269\u3001\u9577\u3044\u5e73\u8861\u5316\u6642\u9593\u3001\u30b7\u30b9\u30c6\u30e0\u306e\u6c5a\u67d3\u306a\u3069\uff09\u3092\u5f15\u304d\u8d77\u3053\u3059\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>Raptor Polar X\u30ab\u30e9\u30e0\u3092\u7528\u3044\u308b\u3068\u3001\u65b0\u898f\u56fa\u5b9a\u76f8\u304c\u6975\u6027\u30a2\u30cb\u30aa\u30f3\u6027\u5316\u5408\u7269\u3068\u3088\u308a\u5f37\u3044\u76f8\u4e92\u4f5c\u7528\u3092\u63d0\u4f9b\u3059\u308b\u305f\u3081\u3001\u3088\u308a\u826f\u3044\u89e3\u6c7a\u624b\u6bb5\u3068\u306a\u308a\u307e\u3059\u3002\u3053\u308c\u306f\u30cf\u30a4\u30d6\u30ea\u30c3\u30c9\u4fdd\u6301\u30e2\u30fc\u30c9\uff08HILIC\u304a\u3088\u3073\u30a4\u30aa\u30f3\u4ea4\u63db\uff09\u3092\u7279\u5fb4\u3068\u3057\u3001\u5f37\u3044\u4fdd\u6301\u3092\u63d0\u4f9b\u3057\u307e\u3059\u304c\u3001\u5358\u7d14\u306a\u79fb\u52d5\u76f8\u5909\u5316\u3092\u901a\u3057\u3066\u3001\u5206\u6790\u7a2e\u3092\u5bfe\u79f0\u306a\u30d4\u30fc\u30af\u3068\u3057\u3066\u8fc5\u901f\u306b\u6eb6\u51fa\u3055\u305b\u308b\u3053\u3068\u3092\u53ef\u80fd\u306b\u3057\u307e\u3059\u3002\u30db\u30a6\u30ec\u30f3\u30bd\u30a6\u4e2d\u306e\u6975\u6027\u8fb2\u85ac\u306e\u3053\u306e\u5206\u6790\u3067\u306f\u3001\u30c8\u30fc\u30bf\u30eb\u5206\u6790\u6642\u9593\u304c8\u5206\u30673\u5316\u5408\u7269\u5168\u3066\u306b\u5bfe\u3057\u3066\u512a\u308c\u305f\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u6027\u80fd\u304c\u898b\u3089\u308c\u307e\u3059\u3002 \u3055\u3089\u306b\u3001\u30d4\u30fc\u30af\u5f62\u72b6\u304a\u3088\u3073\u4fdd\u6301\u6642\u9593\u306f\u6570\u767e\u56de\u306e\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u6ce8\u5165\u306b\u308f\u305f\u3063\u3066\u5b89\u5b9a\u3057\u3066 \u304a\u308a\u3001\u7c21\u5358\u306a\u30ac\u30fc\u30c9\u30ab\u30e9\u30e0\u306e\u4ea4\u63db\u306b\u3088\u308a\u5b8c\u5168\u306b\u6027\u80fd\u304c\u56de\u5fa9\u3057\u307e\u3059\u3002\u3053\u306e\u8fc5\u901f\u304b\u3064\u3001\u7c21\u5358\u3067\u3001\u52b9\u679c\u7684\u306a\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u306f\u3001\u8907\u96d1\u3067\u6642\u9593\u306e\u304b\u304b\u308b\u624b\u9806\u3092\u5fc5\u8981\u3068\u305b\u305a\u306b\u3001\u6975\u6027\u8fb2\u85ac\u306e\u76f4\u63a5\u5206\u6790\u306b\u95a2\u5fc3\u306e\u3042\u308b\u98df\u54c1\u30e9\u30dc\u306b\u3068\u3063\u3066\u3088\u3044\u4ee3\u66ff\u6cd5\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0547.png\" alt=\"Polar Pesticides in Spinach on Raptor Polar X by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0547<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 3<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">AMPA<\/td><td class=\"oth\">100<\/td><td class=\"oth\">110.10<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">63.10<\/td><td class=\"oth\">81.10<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glufosinate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51276-47-2\/Glufosinate\" target=\"_blank\" rel=\"noopener\">Glufosinate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">180.20<\/td><td class=\"oth\">85.15<\/td><td class=\"oth\">95.10<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glyphosate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1071-83-6\/Glyphosate\" target=\"_blank\" rel=\"noopener\">Glyphosate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">168.10<\/td><td class=\"oth\">63.05<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A32\" rel=\"noopener\">cat.# 9311A32<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>30 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor Polar X guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A0252\" rel=\"noopener\">cat.# 9311A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Spinach extract<\/td><\/tr>\n<td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water,  0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>5.0<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.5<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.51<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>8<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Frozen spinach was added to a Blixer processor with dry ice (3:1-4:1 ratio) and then ground into a very fine powder. The homogenate was placed into the freezer immediately. A 5.0 gram sample of the spinach powder was weighed into a 50 mL centrifuge tube (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/25846\" rel=\"noopener\">cat.# 25846<\/a>). According to the QuPPe method (Quick Polar Pesticides Method), 5.0 mL of methanol with 1.0% formic acid was added into the centrifuge tube. The tube was shaken by hand for 1 min and then by a mechanical shaker vigorously for 5 min. After centrifuging for 10 min at 4200 rpm, the supernatant was filtered through a 0.22 \u03bcm filter (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/23984\" rel=\"noopener\">cat.# 23984<\/a>). The final extract was fortified with the AMPA, glufosinate, and glyphosate at a final concentration of 100 ng\/mL.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-related-skus\"><h4>Related SKUs<\/h4><ul><li>9311A0252<\/li><li>9311A32<\/li><li>25846<\/li><li>23984<\/li><\/ul><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0547.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">\u3053\u306e\u8a18\u4e8b\u3067\u7d39\u4ecb\u3057\u305f\u88fd\u54c1<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9311A32\">                                \u30ab\u30bf\u30ed\u30b0No. 9311A32                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor Polar X, 2.7 \u00b5m, 30 x 2.1 mm LC\u30ab\u30e9\u30e0<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9311A32\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9311A0252\">                                \u30ab\u30bf\u30ed\u30b0No. 9311A0252                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor Polar X, 2.7 \u00b5m, 5 x 2.1 mm EXP Guard Column Cartridge, 3-pk.<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9311A0252\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/25846\">                                \u30ab\u30bf\u30ed\u30b0No. 25846                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Empty Centrifuge Tubes, 50 mL, Polypropylene w\/Cap, 50-pk.<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/25846\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/23984\">                                \u30ab\u30bf\u30ed\u30b0No. 23984                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">30 mm Syringe Filter, 0.22 \u00b5m, PTFE, White, 100-pk.<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/23984\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u30db\u30a6\u30ec\u30f3\u30bd\u30a6\u4e2d\u306e\u6975\u6027\u8fb2\u85ac\u3092\u76f4\u63a5\u5206\u6790\u3059\u308b\u305f\u3081\u306e\u3053\u306e\u9ad8\u901f\u3067\u30b7\u30f3\u30d7\u30eb\u306aLC-MS \/ MS\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u306f\u3001Raptor Polar 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