{"id":43614,"date":"2022-08-17T00:00:00","date_gmt":"2022-08-17T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/multi-mycotoxin-method-for-alternaria-toxins-ergot-alkaloid-epimers-and-other-major-mycotoxins-in-food\/"},"modified":"2026-01-28T15:56:56","modified_gmt":"2026-01-28T15:56:56","slug":"multi-mycotoxin-method-for-alternaria-toxins-ergot-alkaloid-epimers-and-other-major-mycotoxins-in-food","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/articles-ja\/fsfa3904\/multi-mycotoxin-method-for-alternaria-toxins-ergot-alkaloid-epimers-and-other-major-mycotoxins-in-food","title":{"rendered":"Multi-Mycotoxin Method for <em>Alternaria<\/em> Toxins, Ergot Alkaloid Epimers, and Other Major Mycotoxins in Food"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Increase lab productivity by analyzing emerging mycotoxins using the same method as priority regulated mycotoxins.<\/li>\n\n\n\n<li>No separate run under high pH conditions\u2014Raptor Biphenyl column allows quantitative analysis of all groups under MS-friendly, acidic conditions.<\/li>\n\n\n\n<li>Simple, universal sample prep saves time and does not require further purification procedures.<\/li>\n<\/ul>\n\n\n\n<p>As more mycotoxins come under regulatory purview, new methods are needed to help food safety labs continue to operate efficiently. Comprehensive, multi-mycotoxin methods are an attractive alternative to separate methods for different analyte lists, but they can be difficult to develop due to the wide range of chemical characteristics among mycotoxin classes. In particular, the&nbsp;<em>Alternaria<\/em>&nbsp;toxins and ergot alkaloids create additional challenges for method developers. These emerging mycotoxin food contaminants are unique in that, when analyzed on a C18 column, high pH conditions must be used to obtain acceptable peak shape for the&nbsp;<em>Alternaria<\/em>&nbsp;toxins and adequate separation of the ergot alkaloid epimers. Use of high pH conditions is stressful for LC columns and not suitable for analyzing other classes of mycotoxins, so another approach is required for a truly comprehensive method.<\/p>\n\n\n\n<p>Here, we show the simultaneous analysis of&nbsp;<em>Alternaria<\/em>&nbsp;toxins, ergot alkaloid epimers, and other major mycotoxins using a simple sample preparation procedure and a Raptor Biphenyl column run under acidic conditions. Under these column- and MS-friendly conditions, accurate quantitative results were obtained in a fast, 11-minute total cycle time run for 37 regulated and emerging mycotoxins (including the&nbsp;<em>Alternaria<\/em>&nbsp;toxins and all six essential ergot alkaloids and their epimers). In a separate study, method performance was verified in wheat baby cereal, peanut, tomato puree, and blended flour to demonstrate the applicability of this method to a wide range of food types [1]. Quantitation was performed using matrix-matched standards because isotopically labeled standards were not available for most mycotoxins. Use of this Raptor Biphenyl-based, multi-mycotoxin method provides food safety labs with a means of significantly improving productivity compared to using separate methods for different mycotoxin panels.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong>\u00a0Increase productivity using one multi-mycotoxin method for concurrent analysis of 37 major mycotoxins, including\u00a0<em>Alternaria<\/em>\u00a0toxins and ergot alkaloid epimers (blended flour sample).<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0550.png\" alt=\"Alternaria Toxins, Ergot Alkaloid Epimers, and Other Major Mycotoxins in Fortified Flour Sample on Raptor Biphenyl by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0550<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nivalenol \" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/23282-20-4\/Nivalenol \" target=\"_blank\" rel=\"noopener\">Nivalenol <\/a><\/td><td class=\"oth\">0.92<\/td><td class=\"oth\">295.1<\/td><td class=\"oth\">137.1<\/td><td class=\"oth\">91.0<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Patulin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/149-29-1\/Patulin\" target=\"_blank\" rel=\"noopener\">Patulin<\/a><\/td><td class=\"oth\">1.03<\/td><td class=\"oth\">155.0<\/td><td class=\"oth\">99.0<\/td><td class=\"oth\">81.0<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergometrine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/60-79-7\/Ergometrine\" target=\"_blank\" rel=\"noopener\">Ergometrine<\/a><\/td><td class=\"oth\">1.27<\/td><td class=\"oth\">326.2<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">208.1<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Deoxynivalenol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51481-10-8\/Deoxynivalenol\" target=\"_blank\" rel=\"noopener\">Deoxynivalenol<\/a><\/td><td class=\"oth\">1.30<\/td><td class=\"oth\">297.2<\/td><td class=\"oth\">231.0<\/td><td class=\"oth\">249.0<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergometrinine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/479-00-5\/Ergometrinine\" target=\"_blank\" rel=\"noopener\">Ergometrinine<\/a><\/td><td class=\"oth\">1.83<\/td><td class=\"oth\">326.2<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">208.1<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Fusarenon-X\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/23255-69-8\/Fusarenon-X\" target=\"_blank\" rel=\"noopener\">Fusarenon-X<\/a><\/td><td class=\"oth\">1.98<\/td><td class=\"oth\">355.1<\/td><td class=\"oth\">137.1<\/td><td class=\"oth\">247.1<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 15-Acetyldeoxynivalenol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/88337-96-6\/15-Acetyldeoxynivalenol\" target=\"_blank\" rel=\"noopener\">15-Acetyldeoxynivalenol<\/a><\/td><td class=\"oth\">3.14<\/td><td class=\"oth\">339.2<\/td><td class=\"oth\">137.1<\/td><td class=\"oth\">321.2<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\">3-Acetyldeoxynivalenol<\/td><td class=\"oth\">3.21<\/td><td class=\"oth\">339.2<\/td><td class=\"oth\">213.1<\/td><td class=\"oth\">231.1<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\">Tenuazonic acid<\/td><td class=\"oth\">4.22<\/td><td class=\"oth\">198.1<\/td><td class=\"oth\">125.0<\/td><td class=\"oth\">153.1<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Altenuene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/29752-43-0\/Altenuene\" target=\"_blank\" rel=\"noopener\">Altenuene<\/a><\/td><td class=\"oth\">4.70<\/td><td class=\"oth\">293.2<\/td><td class=\"oth\">257.1<\/td><td class=\"oth\">275.2<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Alternariol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/641-38-3\/Alternariol\" target=\"_blank\" rel=\"noopener\">Alternariol<\/a><\/td><td class=\"oth\">5.30<\/td><td class=\"oth\">259.0<\/td><td class=\"oth\">185.1<\/td><td class=\"oth\">130.0<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Citrinin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/518-75-2\/Citrinin\" target=\"_blank\" rel=\"noopener\">Citrinin<\/a><\/td><td class=\"oth\">5.43<\/td><td class=\"oth\">251.2<\/td><td class=\"oth\">233.1<\/td><td class=\"oth\">205.1<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergosine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/561-94-4\/Ergosine\" target=\"_blank\" rel=\"noopener\">Ergosine<\/a><\/td><td class=\"oth\">5.47<\/td><td class=\"oth\">548.4<\/td><td class=\"oth\">208.1<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\">Fumonisin B1<\/td><td class=\"oth\">5.63<\/td><td class=\"oth\">722.5<\/td><td class=\"oth\">352.3<\/td><td class=\"oth\">334.2<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergosinine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/596-88-3\/Ergosinine\" target=\"_blank\" rel=\"noopener\">Ergosinine<\/a><\/td><td class=\"oth\">5.67<\/td><td class=\"oth\">548.4<\/td><td class=\"oth\">208.1<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Diacetoxyscirpenol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2270-40-8\/Diacetoxyscirpenol\" target=\"_blank\" rel=\"noopener\">Diacetoxyscirpenol<\/a><\/td><td class=\"oth\">5.73<\/td><td class=\"oth\">384.2<\/td><td class=\"oth\">247.1<\/td><td class=\"oth\">307.2<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergotamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/113-15-5\/Ergotamine\" target=\"_blank\" rel=\"noopener\">Ergotamine<\/a><\/td><td class=\"oth\">5.90<\/td><td class=\"oth\">582.4<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">268.2<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocornine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/564-36-3\/Ergocornine\" target=\"_blank\" rel=\"noopener\">Ergocornine<\/a><\/td><td class=\"oth\">6.03<\/td><td class=\"oth\">562.4<\/td><td class=\"oth\">268.2<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">19.<\/td><td class=\"cmpd\">Ergotaminine<\/td><td class=\"oth\">6.13<\/td><td class=\"oth\">582.4<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">268.2<\/td><\/tr>\n<tr><td class=\"num\">20.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for HT-2\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/26934-87-2\/HT-2\" target=\"_blank\" rel=\"noopener\">HT-2<\/a><\/td><td class=\"oth\">6.20<\/td><td class=\"oth\">447.2<\/td><td class=\"oth\">345.1<\/td><td class=\"oth\">285.1<\/td><\/tr>\n<tr><td class=\"num\">21.<\/td><td class=\"cmpd\">Fumonisin B3<\/td><td class=\"oth\">6.32<\/td><td class=\"oth\">706.4<\/td><td class=\"oth\">336.2<\/td><td class=\"oth\">318.3<\/td><\/tr>\n<tr><td class=\"num\">22.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocryptine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/511-09-1\/Ergocryptine\" target=\"_blank\" rel=\"noopener\">Ergocryptine<\/a><\/td><td class=\"oth\">6.32<\/td><td class=\"oth\">576.4<\/td><td class=\"oth\">268.2<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">23.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocristine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/511-08-0\/Ergocristine\" target=\"_blank\" rel=\"noopener\">Ergocristine<\/a><\/td><td class=\"oth\">6.56<\/td><td class=\"oth\">610.4<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">592.4<\/td><\/tr>\n<tr><td class=\"num\">24.<\/td><td class=\"cmpd\">Fumonisin B2<\/td><td class=\"oth\">6.68<\/td><td class=\"oth\">706.4<\/td><td class=\"oth\">336.2<\/td><td class=\"oth\">318.3<\/td><\/tr>\n<tr><td class=\"num\">25.<\/td><td class=\"cmpd\">Tentoxin<\/td><td class=\"oth\">6.70<\/td><td class=\"oth\">415.2<\/td><td class=\"oth\">312.2<\/td><td class=\"oth\">302.2<\/td><\/tr>\n<tr><td class=\"num\">26.<\/td><td class=\"cmpd\">\u03b1-Zearalenol<\/td><td class=\"oth\">6.96<\/td><td class=\"oth\">303.1<\/td><td class=\"oth\">285.1<\/td><td class=\"oth\">175.0<\/td><\/tr>\n<tr><td class=\"num\">27.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocorninine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/564-37-4\/Ergocorninine\" target=\"_blank\" rel=\"noopener\">Ergocorninine<\/a><\/td><td class=\"oth\">7.07<\/td><td class=\"oth\">562.4<\/td><td class=\"oth\">268.2<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">28.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for T-2\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-31-7\/T-2\" target=\"_blank\" rel=\"noopener\">T-2<\/a><\/td><td class=\"oth\">7.14<\/td><td class=\"oth\">489.2<\/td><td class=\"oth\">387.1<\/td><td class=\"oth\">245.1<\/td><\/tr>\n<tr><td class=\"num\">29.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aflatoxin G2\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/7241-98-7\/Aflatoxin G2\" target=\"_blank\" rel=\"noopener\">Aflatoxin G2<\/a><\/td><td class=\"oth\">7.15<\/td><td class=\"oth\">331.2<\/td><td class=\"oth\">189.0<\/td><td class=\"oth\">313.0<\/td><\/tr>\n<tr><td class=\"num\">30.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocryptinine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/511-10-4\/Ergocryptinine\" target=\"_blank\" rel=\"noopener\">Ergocryptinine<\/a><\/td><td class=\"oth\">7.31<\/td><td class=\"oth\">576.4<\/td><td class=\"oth\">268.2<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">31.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocristinine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/511-07-9\/Ergocristinine\" target=\"_blank\" rel=\"noopener\">Ergocristinine<\/a><\/td><td class=\"oth\">7.53<\/td><td class=\"oth\">610.4<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">592.4<\/td><\/tr>\n<tr><td class=\"num\">32.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aflatoxin G1\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1165-39-5\/Aflatoxin G1\" target=\"_blank\" rel=\"noopener\">Aflatoxin G1<\/a><\/td><td class=\"oth\">7.62<\/td><td class=\"oth\">329.1<\/td><td class=\"oth\">199.7<\/td><td class=\"oth\">243.0<\/td><\/tr>\n<tr><td class=\"num\">33.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Zearalenone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/17924-92-4\/Zearalenone\" target=\"_blank\" rel=\"noopener\">Zearalenone<\/a><\/td><td class=\"oth\">7.65<\/td><td class=\"oth\">319.2<\/td><td class=\"oth\">283.1<\/td><td class=\"oth\">187.0<\/td><\/tr>\n<tr><td class=\"num\">34.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Alternariol monomethylether\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/26894-49-5\/Alternariol monomethylether\" target=\"_blank\" rel=\"noopener\">Alternariol monomethylether<\/a><\/td><td class=\"oth\">7.69<\/td><td class=\"oth\">273.0<\/td><td class=\"oth\">199.1<\/td><td class=\"oth\">128.0<\/td><\/tr>\n<tr><td class=\"num\">35.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aflatoxin B2\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/7220-81-7\/Aflatoxin B2\" target=\"_blank\" rel=\"noopener\">Aflatoxin B2<\/a><\/td><td class=\"oth\">7.81<\/td><td class=\"oth\">315.1<\/td><td class=\"oth\">287.0<\/td><td class=\"oth\">259.0<\/td><\/tr>\n<tr><td class=\"num\">36.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aflatoxin B1\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1162-65-8\/Aflatoxin B1\" target=\"_blank\" rel=\"noopener\">Aflatoxin B1<\/a><\/td><td class=\"oth\">8.20<\/td><td class=\"oth\">313.2<\/td><td class=\"oth\">241.1<\/td><td class=\"oth\">284.9<\/td><\/tr>\n<tr><td class=\"num\">37.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ochratoxin A\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/303-47-9\/Ochratoxin A\" target=\"_blank\" rel=\"noopener\">Ochratoxin A<\/a><\/td><td class=\"oth\">8.31<\/td><td class=\"oth\">404.1<\/td><td class=\"oth\">239.0<\/td><td class=\"oth\">358.0<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0550\" rel=\"noopener\">cat.# 9309A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0550\" rel=\"noopener\">cat.# 9309A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>60 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><td><\/td><td>Aflatoxins standard (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/34121?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0550\" rel=\"noopener\">cat.# 34121<\/a>)<\/td><\/tr><tr><td><\/td><td>Ochratoxin A standard (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/34122?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0550\" rel=\"noopener\">cat.# 34122<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>50:50 Water:methanol<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>6.25 ng\/mL final concentration after sample preparation<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 0.05% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol, 0.05% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>75<\/td><td>25<\/td><\/tr><tr><td>5.00<\/td><td>0.4<\/td><td>50<\/td><td>50<\/td><\/tr><tr><td>9.00<\/td><td>0.4<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>9.01<\/td><td>0.4<\/td><td>75<\/td><td>25<\/td><\/tr><tr><td>11.0<\/td><td>0.4<\/td><td>75<\/td><td>25<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>A blended flour was prepared by mixing white rice flour (75%); brown rice flour (5%); millet flour (5%); oat flour (5%); all-purpose wheat flour (5%); and all-purpose, gluten-free flour (5%). Two grams of the flour sample were weighed into a 50-mL polypropylene centrifuge tube (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/25846?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0550\" rel=\"noopener\">cat.# 25846<\/a>) and fortified at 50 \u00b5g\/kg for all analytes with a stock standard solution. After sitting at room temperature for 10 minutes, 16 mL of extraction solution (80:20 acetonitrile:water) containing 0.5% formic acid were added, and the tube was stirred to create a homogenous suspension. The extraction was carried out by shaking horizontally on a digital pulse mixer (Glas-Col LLC, Terre Haute, IN) at 800 rpm for 20 minutes. After centrifuging for 5 minutes at 4000 rpm, 1 mL of extract was evaporated to dryness at 45 \u00b0C under a gentle stream of nitrogen. The dried extract was reconstituted with 1 mL of 50:50 water:methanol solution, and a 0.4 mL aliquot was transferred to and filtered using a Thomson SINGLE StEP filter vial with a 0.2 \u00b5m PTFE filter cat.# 25874. Five \u00b5L of the filtered solution was injected for the LC-MS\/MS analysis.<br \/><br \/><\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>The chromatogram shows peaks with the MS transition of product ion 1.<br \/><br \/>Note that method development work demonstrated that whenever a new column is installed it must be rinsed and maintained under mobile phase overnight to ensure an acceptable peak shape for tenuazonic acid.  <br \/><br \/>Want even better performance when analyzing metal-sensitive compounds? Check out Inert LC columns at <a href=\"https:\/\/www.restek.com\/inert\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a>.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0550.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>References<\/strong><\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li>H. Liang, J. York, J. Konschnik, H. Majer, J. Steimling,&nbsp;<a href=\"https:\/\/www.restek.com\/articles\/comprehensive-mycotoxin-analysis-simultaneous-determination-of-alternaria-toxins-ergot-alkaloid-epimers-and-other-major-mycotoxins-in-various-food-matrices-by-lc-msms\" target=\"_blank\" rel=\"noopener\">Comprehensive mycotoxin analysis: simultaneous determination of&nbsp;<em>Alternaria<\/em>&nbsp;toxins, ergot alkaloid epimers, and other major mycotoxins in various food matrices by LC-MS\/MS<\/a>, Restek Corporation, 2022.<\/li>\n<\/ol>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\" style=\"background-color:#e4f7fa\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-1489b0bd8c7fa6a213f7f4f1cf134d8f\" style=\"color:#02366d\">Want even better performance when analyzing mycotoxins and other metal-sensitive compounds?<\/h3>\n\n\n\n<p>Learn more at&nbsp;<a href=\"https:\/\/www.restek.com\/articles\/accurately-analyze-metal-sensitive-compounds-with-resteks-new-inert-lc-columns\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\"><div class=\"wp-block-image\">\n<figure class=\"alignright size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"400\" height=\"123\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02.png\" alt=\"\" class=\"wp-image-29764\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02.png 400w, https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02-300x92.png 300w\" sizes=\"auto, (max-width: 400px) 100vw, 400px\" \/><\/figure>\n<\/div><\/div>\n<\/div>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Labs analyzing mycotoxins in food can increase productivity with this method for simultaneous quantitative analysis of 37 regulated and emerging mycotoxins&#8211;including Alternaria toxins, ergot alkaloid epimers, and other priority mycotoxins.<\/p>\n","protected":false},"author":11,"featured_media":7033,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[789],"tags":[],"industries-application":[2214,2216],"post-badge":[30],"resource-type":[2623],"product-library":[2463,2445],"resource-technique":[2318,2362],"hf_cat_post":[650,2624],"ppma_author":[414],"class_list":["post-43614","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles-ja","industries-application-industrie-agroalimentaire","industries-application-securite-alimentaire","post-badge-featured","resource-type-featured-application","product-library-colonnes-lc","product-library-produits-pour-la-chromatographie-en-phase-liquide","resource-technique-chromatographie-en-phase-liquide","resource-technique-ms-ms-fr"],"acf":[],"taxonomy_info":{"category":[{"value":789,"label":"\u6280\u8853\u8cc7\u6599"}],"industries-application":[{"value":2214,"label":"Industrie 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