{"id":43616,"date":"2022-08-17T00:00:00","date_gmt":"2022-08-17T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/analysis-of-ergot-alkaloid-mycotoxins-in-blended-flour-by-lc-msms-under-acidic-conditions\/"},"modified":"2026-01-03T17:54:41","modified_gmt":"2026-01-03T17:54:41","slug":"analysis-of-ergot-alkaloid-mycotoxins-in-blended-flour-by-lc-msms-under-acidic-conditions","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/articles-fr\/fsfa3905\/analysis-of-ergot-alkaloid-mycotoxins-in-blended-flour-by-lc-msms-under-acidic-conditions","title":{"rendered":"Analysis of Ergot Alkaloid Mycotoxins in Blended Flour by LC-MS\/MS Under Acidic Conditions"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Baseline separation of six critical ergot alkaloids and their epimers allows definitive quantification.<\/li>\n\n\n\n<li>Fast, 11-min total cycle time supports high-throughput testing.<\/li>\n\n\n\n<li>Acidic conditions suitable for the simultaneous analysis of ergot alkaloids,&nbsp;<em>Alternaria<\/em>&nbsp;toxins, and major regulated mycotoxins.<\/li>\n<\/ul>\n\n\n\n<p>Ergot alkaloids are mycotoxin contaminants of cereal plants that are of increasing regulatory interest due to their widespread presence and the dietary importance of cereals. Of more than 40 known ergot alkaloids, six compounds including ergocornine, ergocristine, ergocryptine, ergometrine, ergosine, and ergotamine, together with their isomeric -inine epimers, are currently regulated by the EU. When these ergot alkaloids are analyzed on a C18 column, high pH conditions are needed to separate all 12 epimers. In contrast, when a Raptor Biphenyl column is used, baseline separation can be achieved under regular column- and MS-friendly, acidic conditions. As shown here, complete resolution of all 12 epimers, including the challenging ergotamine\/ergotaminine and ergosine\/ergosinine epimer pairs, was obtained in 11 minutes of total cycle time.<\/p>\n\n\n\n<p>The use of a Raptor Biphenyl column and acidic conditions also allows concurrent analysis of ergot alkaloids with other mycotoxins of interest. A separate study established that these conditions are suitable for quantitative analysis of ergot alkaloids in the same run as&nbsp;<em>Alternaria<\/em>&nbsp;toxins and priority regulated mycotoxins in a variety of foods [1]. Quantitation in that study was performed in wheat baby cereal, peanut, tomato puree, and blended flour using matrix-matched standards because isotopically labeled standards were not available for these mycotoxins. Suitability of this method for multiresidue mycotoxin testing provides food safety labs with a more efficient workflow and the time-saving benefit of combined analysis.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong>\u00a0Baseline separation of all ergot alkaloid epimers in a blended flour sample was achieved on a Raptor Biphenyl column under acidic conditions.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0551.png\" alt=\"Ergot Alkaloid Epimers in Fortified Flour Sample on Raptor Biphenyl by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0551<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12 peak-50\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergometrine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/60-79-7\/Ergometrine\" target=\"_blank\" rel=\"noopener\">Ergometrine<\/a><\/td><td class=\"oth\">1.27<\/td><td class=\"oth\">326.2<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">208.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergometrinine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/479-00-5\/Ergometrinine\" target=\"_blank\" rel=\"noopener\">Ergometrinine<\/a><\/td><td class=\"oth\">1.83<\/td><td class=\"oth\">326.2<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">208.1<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergosine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/561-94-4\/Ergosine\" target=\"_blank\" rel=\"noopener\">Ergosine<\/a><\/td><td class=\"oth\">5.47<\/td><td class=\"oth\">548.4<\/td><td class=\"oth\">208.1<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergosinine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/596-88-3\/Ergosinine\" target=\"_blank\" rel=\"noopener\">Ergosinine<\/a><\/td><td class=\"oth\">5.67<\/td><td class=\"oth\">548.4<\/td><td class=\"oth\">208.1<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergotamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/113-15-5\/Ergotamine\" target=\"_blank\" rel=\"noopener\">Ergotamine<\/a><\/td><td class=\"oth\">5.90<\/td><td class=\"oth\">582.4<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">268.2<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocornine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/564-36-3\/Ergocornine\" target=\"_blank\" rel=\"noopener\">Ergocornine<\/a><\/td><td class=\"oth\">6.03<\/td><td class=\"oth\">562.4<\/td><td class=\"oth\">268.2<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<\/tbody><\/table>\n<table class=\"peaks col-lg-6 col-12 peak-50\"><thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead><tbody>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\">Ergotaminine<\/td><td class=\"oth\">6.13<\/td><td class=\"oth\">582.4<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">268.2<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocryptine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/511-09-1\/Ergocryptine\" target=\"_blank\" rel=\"noopener\">Ergocryptine<\/a><\/td><td class=\"oth\">6.32<\/td><td class=\"oth\">576.4<\/td><td class=\"oth\">268.2<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocristine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/511-08-0\/Ergocristine\" target=\"_blank\" rel=\"noopener\">Ergocristine<\/a><\/td><td class=\"oth\">6.56<\/td><td class=\"oth\">610.4<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">592.4<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocorninine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/564-37-4\/Ergocorninine\" target=\"_blank\" rel=\"noopener\">Ergocorninine<\/a><\/td><td class=\"oth\">7.07<\/td><td class=\"oth\">562.4<\/td><td class=\"oth\">268.2<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocryptinine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/511-10-4\/Ergocryptinine\" target=\"_blank\" rel=\"noopener\">Ergocryptinine<\/a><\/td><td class=\"oth\">7.31<\/td><td class=\"oth\">576.4<\/td><td class=\"oth\">268.2<\/td><td class=\"oth\">223.2<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ergocristinine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/511-07-9\/Ergocristinine\" target=\"_blank\" rel=\"noopener\">Ergocristinine<\/a><\/td><td class=\"oth\">7.53<\/td><td class=\"oth\">610.4<\/td><td class=\"oth\">223.2<\/td><td class=\"oth\">592.4<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0551\" rel=\"noopener\">cat.# 9309A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor Biphenyl EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A0252?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0551\" rel=\"noopener\">cat.# 9309A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>60 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>50:50 Water:methanol<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>6.25 ng\/mL final concentration after sample preparation<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 0.05% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol, 0.05% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>75<\/td><td>25<\/td><\/tr><tr><td>5.00<\/td><td>0.4<\/td><td>50<\/td><td>50<\/td><\/tr><tr><td>9.00<\/td><td>0.4<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>9.01<\/td><td>0.4<\/td><td>75<\/td><td>25<\/td><\/tr><tr><td>11.0<\/td><td>0.4<\/td><td>75<\/td><td>25<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>A blended flour was prepared by mixing white rice flour (75%); brown rice flour (5%); millet flour (5%); oat flour (5%); all-purpose wheat flour (5%); and all-purpose, gluten-free flour (5%). Two grams of the flour sample were weighed into a 50-mL polypropylene centrifuge tube (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/25846?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FS0551\" rel=\"noopener\">cat.# 25846<\/a>) and fortified at 50 \u00b5g\/kg for all analytes with a stock standard solution. After sitting at room temperature for 10 minutes, 16 mL of extraction solution (80:20 acetonitrile:water) containing 0.5% formic acid were added, and the tube was stirred to create a homogenous suspension. The extraction was carried out by shaking horizontally on a digital pulse mixer (Glas-Col LLC, Terre Haute, IN) at 800 rpm for 20 minutes. After centrifuging for 5 minutes at 4000 rpm, 1 mL of extract was evaporated to dryness at 45 \u00b0C under a gentle stream of nitrogen. The dried extract was reconstituted with 1 mL of 50:50 water:methanol solution, and a 0.4 mL aliquot was transferred to and filtered using a Thomson SINGLE StEP filter vial with a 0.2 \u00b5m PTFE filter cat.# 25874. Five \u00b5L of the filtered solution was injected for the LC-MS\/MS analysis.<br \/><br \/><\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>Want even better performance when analyzing metal-sensitive compounds? Check out Inert LC columns at <a href=\"https:\/\/www.restek.com\/inert\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a>.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0551.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\"><strong>References<\/strong><\/h2>\n\n\n\n<ol class=\"wp-block-list\">\n<li>S.H. Liang, J. York, J. Konschnik, H. Majer, J. Steimling,&nbsp;<a href=\"https:\/\/discover.restek.com\/FSFA3904\/multi-mycotoxin-method-for-alternaria-toxins-ergot-alkaloid-epimers-and-other-major-mycotoxins-in-food\/\" data-type=\"post\" data-id=\"43614\">Comprehensive mycotoxin analysis: simultaneous determination of&nbsp;<em>Alternaria<\/em>&nbsp;toxins, ergot alkaloid epimers, and other major mycotoxins in various food matrices by LC-MS\/MS<\/a>, Restek Corporation, 2022.<\/li>\n<\/ol>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\" style=\"background-color:#e4f7fa\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<h3 class=\"wp-block-heading has-text-color has-link-color wp-elements-1489b0bd8c7fa6a213f7f4f1cf134d8f\" style=\"color:#02366d\">Want even better performance when analyzing mycotoxins and other metal-sensitive compounds?<\/h3>\n\n\n\n<p>Learn more at&nbsp;<a href=\"https:\/\/www.restek.com\/articles\/accurately-analyze-metal-sensitive-compounds-with-resteks-new-inert-lc-columns\" target=\"_blank\" rel=\"noopener\">www.restek.com\/inert<\/a><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\"><div class=\"wp-block-image\">\n<figure class=\"alignright size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"400\" height=\"123\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02.png\" alt=\"\" class=\"wp-image-29764\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02.png 400w, https:\/\/discover.restek.com\/wp-content\/uploads\/teaser-product-lc-columns-inert-lc-02-300x92.png 300w\" sizes=\"auto, (max-width: 400px) 100vw, 400px\" \/><\/figure>\n<\/div><\/div>\n<\/div>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Six major ergot alkaloids and their corresponding epimers can be baseline resolved under low pH conditions using a Raptor Biphenyl column. The following conditions also allow Alternaria toxins and other priority mycotoxins to be analyzed concurrently.<\/p>\n","protected":false},"author":11,"featured_media":7039,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[462],"tags":[],"industries-application":[2214,2216],"post-badge":[],"resource-type":[2623],"product-library":[2463,2445,2447,2474],"resource-technique":[2318,2362],"hf_cat_post":[650,2624],"ppma_author":[414],"class_list":["post-43616","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles-fr","industries-application-industrie-agroalimentaire","industries-application-securite-alimentaire","resource-type-featured-application","product-library-colonnes-lc","product-library-produits-pour-la-chromatographie-en-phase-liquide","product-library-produits-pour-la-preparation-dechantillons","product-library-spe-sle-fr","resource-technique-chromatographie-en-phase-liquide","resource-technique-ms-ms-fr"],"acf":[],"taxonomy_info":{"category":[{"value":462,"label":"Articles"}],"industries-application":[{"value":2214,"label":"Industrie Agroalimentaire"},{"value":2216,"label":"S\u00e9curit\u00e9 alimentaire"}],"resource-type":[{"value":2623,"label":"Featured Application"}],"product-library":[{"value":2463,"label":"Colonnes LC"},{"value":2445,"label":"Produits pour la chromatographie en phase liquide"},{"value":2447,"label":"Produits pour la pr\u00e9paration d'\u00e9chantillons"},{"value":2474,"label":"SPE et SLE"}],"resource-technique":[{"value":2318,"label":"Chromatographie en phase liquide"},{"value":2362,"label":"MS\/MS"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-FSFA3905-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/ja\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":462,"name":"Articles","slug":"articles-fr","term_group":0,"term_taxonomy_id":462,"taxonomy":"category","description":"","parent":0,"count":467,"filter":"raw","cat_ID":462,"category_count":467,"category_description":"","cat_name":"Articles","category_nicename":"articles-fr","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/43616","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/comments?post=43616"}],"version-history":[{"count":6,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/43616\/revisions"}],"predecessor-version":[{"id":79445,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/43616\/revisions\/79445"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media\/7039"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media?parent=43616"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/categories?post=43616"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/tags?post=43616"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/industries-application?post=43616"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/post-badge?post=43616"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-type?post=43616"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/product-library?post=43616"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-technique?post=43616"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/hf_cat_post?post=43616"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/ppma_author?post=43616"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}