{"id":45333,"date":"2025-10-08T17:54:42","date_gmt":"2025-10-08T17:54:42","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/a-comparison-of-sample-preparation-techniques-for-drugs-of-abuse-in-oral-fluid-japan\/"},"modified":"2026-01-14T19:22:14","modified_gmt":"2026-01-14T19:22:14","slug":"a-comparison-of-sample-preparation-techniques-for-drugs-of-abuse-in-oral-fluid-japan","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/application-notes-ja\/cfan4466-ja\/a-comparison-of-sample-preparation-techniques-for-drugs-of-abuse-in-oral-fluid-japan","title":{"rendered":"\u53e3\u8154\u6db2\u4e2d\u306e\u85ac\u7269\u5206\u6790\u306b\u304a\u3051\u308b\u9769\u65b0\uff1aSALLE\u3068SLE\u3067\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u3092\u52b9\u7387\u5316"},"content":{"rendered":"\n

\u6982\u8981<\/h2>\n\n\n\n

\u4e71\u7528\u85ac\u7269\u306e\u691c\u67fb\u306f\u3001\u6bd2\u7269\u5b66\u3084\u8077\u5834\u3067\u306e\u85ac\u7269\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u306a\u3069\u3001\u3055\u307e\u3056\u307e\u306a\u5206\u91ce\u3067\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u307e\u3059\u3002\u5f93\u6765\u306f\u8840\u6db2\u3084\u5c3f\u304c\u6a19\u6e96\u7684\u306a\u691c\u4f53\u3068\u3057\u3066\u7528\u3044\u3089\u308c\u3066\u304d\u307e\u3057\u305f\u304c\u3001\u3053\u308c\u3089\u306e\u63a1\u53d6\u306f\u8eab\u4f53\u3078\u306e\u8ca0\u62c5\u304c\u5927\u304d\u304f\u3001\u88ab\u9a13\u8005\u306b\u3068\u3063\u3066\u3082\u30b9\u30c8\u30ec\u30b9\u3068\u306a\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u3046\u3057\u305f\u80cc\u666f\u304b\u3089\u3001\u8fd1\u5e74\u306f\u53e3\u8154\u6db2\uff08\u553e\u6db2\uff09\u3092\u7528\u3044\u305f\u691c\u67fb\u304c\u6ce8\u76ee\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u53e3\u8154\u6db2\u306f\u3001\u7c21\u5358\u306a\u65b9\u6cd5\u3067\u88ab\u9a13\u8005\u306b\u30b9\u30c8\u30ec\u30b9\u3092\u4e0e\u3048\u305a\u63a1\u53d6\u53ef\u80fd\u3067\u3042\u308b\u3053\u3068\u304b\u3089\u3001\u3088\u308a\u591a\u304f\u306e\u73fe\u5834\u3067\u5229\u7528\u304c\u5e83\u304c\u3063\u3066\u3044\u307e\u3059\u3002\u4e00\u65b9\u3067\u3001\u53e3\u8154\u6db2\u691c\u67fb\u306b\u306f\u8ab2\u984c\u3082\u3042\u308a\u307e\u3059\u3002\u7279\u306b\u3001\u63a1\u53d6\u306b\u7528\u3044\u308b\u88c5\u7f6e\u306e\u7de9\u885d\u6db2\u306b\u306f\u4fdd\u5b58\u6599\u3084\u754c\u9762\u6d3b\u6027\u5264\u3068\u3044\u3063\u305f\u6dfb\u52a0\u5264\u304c\u542b\u307e\u308c\u3066\u304a\u308a\u3001\u3053\u308c\u3089\u304c\u5206\u6790\u306b\u5e72\u6e09\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u3053\u308c\u3089\u306e\u6210\u5206\u3092\u9664\u53bb\u3059\u308b\u524d\u51e6\u7406\u3068\u3057\u3066\u56fa\u76f8\u62bd\u51fa\uff08SPE\uff09\u5de5\u7a0b\u304c\u5fc5\u8981\u3067\u3059\u304c\u3001\u3053\u306e\u5de5\u7a0b\u306b\u306f\u73fe\u72b6\u3001\u591a\u304f\u306e\u6642\u9593\u3068\u624b\u9593\u304c\u304b\u304b\u3063\u3066\u3044\u308b\u306e\u304c\u5b9f\u60c5\u3067\u3059\u3002\u305d\u3053\u3067\u3001\u4eca\u56de\u306e\u7814\u7a76\u3067\u306f\u3001LC-MS\/MS\u3092\u7528\u3044\u305f\u53e3\u8154\u6db2\u4e2d\u306e\u4e71\u7528\u85ac\u7269\u304a\u3088\u3073\u65b0\u898f\u7cbe\u795e\u6d3b\u6027\u7269\u8cea\u306e\u5206\u6790\u306b\u304a\u3044\u3066\u3001\u5869\u6790\u652f\u63f4\u6db2\u6db2\u62bd\u51fa\uff08SALLE\uff09\u3001\u4fdd\u6301\u578b\u6db2\u6db2\u62bd\u51fa\uff08SLE\uff09\u3001\u304a\u3088\u3073\u5e0c\u91c8\u6ce8\u5165\u3068\u3044\u30463\u3064\u306e\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u6280\u8853\u3092\u6bd4\u8f03\u3057\u3001SPE\u5de5\u7a0b\u306e\u6642\u9593\u77ed\u7e2e\u3092\u76ee\u6307\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u306f\u3058\u3081\u306b<\/h2>\n\n\n\n

\u7c21\u4fbf\u304b\u3064\u88ab\u9a13\u8005\u306b\u30b9\u30c8\u30ec\u30b9\u3092\u4e0e\u3048\u305a\u306b\u63a1\u53d6\u53ef\u80fd\u306a\u305f\u3081\u3001\u3088\u308a\u591a\u304f\u306e\u73fe\u5834\u3067\u5229\u7528\u304c\u5e83\u304c\u308b\u53e3\u8154\u6db2\u691c\u67fb[1]\u3067\u3059\u304c\u3001\u53e3\u8154\u6db2\u306f\u3001\u553e\u6db2\u305d\u306e\u3082\u306e\u3060\u3051\u3067\u306a\u304f\u3001\u53e3\u8154\u5185\u306e\u7d30\u83cc\u3084\u98df\u3079\u304b\u3059\u306a\u3069\u3082\u542b\u3080\u8907\u96d1\u306a\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u3067\u3059[2]\u3002\u8840\u6db2\u3068\u826f\u597d\u306a\u85ac\u7269\u6fc3\u5ea6\u306e\u76f8\u95a2\u304c\u5f97\u3089\u308c\u308b\u4e00\u65b9\u3067\u3001\u98f2\u98df\u3084\u55ab\u7159\u306a\u3069\u3001\u63a1\u53d6\u524d\u306e\u751f\u6d3b\u7fd2\u6163\u304c\u691c\u67fb\u7d50\u679c\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u70b9\u306b\u306f\u6ce8\u610f\u304c\u5fc5\u8981\u3067\u3059[1]\u3002\u307e\u305f\u3001\u5c3f\u691c\u67fb\u3067\u61f8\u5ff5\u3055\u308c\u308b\u3088\u3046\u306a\u3001\u691c\u4f53\u3078\u306e\u7570\u7269\u6df7\u5165\uff08\u4e0d\u7d14\u7269\uff09\u3084\u6c34\u306a\u3069\u306b\u3088\u308b\u5e0c\u91c8\u3001\u4ed6\u4eba\u306e\u5c3f\u3068\u3059\u308a\u66ff\u3048\u308b\u3068\u3044\u3063\u305f\u4e0d\u6b63\u306e\u30ea\u30b9\u30af\u304c\u6bd4\u8f03\u7684\u5c11\u306a\u3044\u3053\u3068\u3082\u3001\u53e3\u8154\u6db2\u691c\u67fb\u306e\u5229\u70b9\u306e\u3072\u3068\u3064\u3067\u3059[1,2]\u3002<\/p>\n\n\n\n

\u53e3\u8154\u6db2\u3092\u7528\u3044\u305f\u85ac\u7269\u5206\u6790\u3067\u306f\u3001\u901a\u5e38\u3001\u56fa\u76f8\u62bd\u51fa\uff08SPE\uff09\u3084\u5e0c\u91c8\u6ce8\u5165\u6cd5\u304c\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u3068\u3057\u3066\u7528\u3044\u3089\u308c\u307e\u3059\u3002\u53e3\u8154\u6db2\u81ea\u4f53\u306f\u6bd4\u8f03\u7684\u4f5c\u696d\u3057\u3084\u3059\u3044\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u3067\u3059\u304c\u3001\u63a1\u53d6\u30ad\u30c3\u30c8\u306e\u7de9\u885d\u6db2\u306b\u542b\u307e\u308c\u308b\u6dfb\u52a0\u5264\u304c\u554f\u984c\u3067\u3059\u3002\u7de9\u885d\u6db2\u306f\u30b5\u30f3\u30d7\u30eb\u4fdd\u5b58\u3068\u5fae\u751f\u7269\u306e\u5897\u6b96\u6291\u5236\u306b\u5fc5\u8981\u306a\u3082\u306e\u3067\u3059\u304c\u3001\u7de9\u885d\u6db2\u306b\u542b\u307e\u308c\u308b\u6dfb\u52a0\u5264\u306f\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u52b9\u679c\u3092\u5f15\u304d\u8d77\u3053\u3057\u3001\u5206\u6790\u30ab\u30e9\u30e0\u306e\u5bff\u547d\u3092\u7e2e\u3081\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002SPE\u306e\u65b9\u304c\u7de9\u885d\u6db2\u306e\u5f71\u97ff\u3092\u8efd\u6e1b\u3067\u304d\u307e\u3059\u304c\u3001\u624b\u9806\u304c\u8907\u96d1\u3067\u6642\u9593\u304c\u304b\u304b\u308b\u3068\u3044\u3046\u30c7\u30e1\u30ea\u30c3\u30c8\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n

\u5b9f\u969b\u306b\u85ac\u7269\u5206\u6790\u3092\u884c\u3046\u30e9\u30dc\u304c\u6c42\u3081\u308b\u306e\u306f\u6642\u9593\u304c\u304b\u304b\u3089\u306a\u3044\u3001\u52b9\u679c\u7684\u306a\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u3067\u3059\u3002\u305d\u3053\u3067\u3001\u3053\u306e\u5b9f\u9a13\u3067\u306f\u3001\u4fdd\u6301\u578b\u6db2\u6db2\u62bd\u51fa\uff08SLE\uff09\u3068\u5869\u6790\u652f\u63f4\u6db2\u6db2\u62bd\u51fa\uff08SALLE\uff09\u304a\u3088\u3073\u3001\u5358\u7d14\u306a\u5e0c\u91c8\u6ce8\u5165\u6cd5\u3068\u3044\u30463\u7a2e\u985e\u306e\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u306b\u3064\u3044\u3066\u6bd4\u8f03\u3092\u884c\u3044\u307e\u3057\u305f\u3002\u306a\u304a\u3001\u5e0c\u91c8\u6ce8\u5165\u6cd5\u3067\u306f\u691c\u51fa\u611f\u5ea6\u304c\u8457\u3057\u304f\u4f4e\u304f\u3001\u5b9a\u91cf\u7684\u8a55\u4fa1\u304c\u56f0\u96e3\u3060\u3063\u305f\u305f\u3081\u3001\u4ee5\u964d\u306e\u691c\u8a0e\u5bfe\u8c61\u304b\u3089\u9664\u5916\u3057\u307e\u3057\u305f\u3002\u4e00\u65b9\u3001SLE\u304a\u3088\u3073SALLE\u3092\u63a1\u7528\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u3067\u306f\u826f\u597d\u306a\u30ec\u30b9\u30dd\u30f3\u30b9\u304c\u5f97\u3089\u308c\u3001\u7cbe\u5ea6\u3001\u518d\u73fe\u6027\u3001\u76f4\u7dda\u6027\u306e\u7d50\u679c\u306f\u826f\u597d\u3067\u3001\u6709\u7528\u6027\u304c\u3042\u308b\u3068\u8a55\u4fa1\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u5b9f\u9a13<\/h2>\n\n\n\n

\u6821\u6b63\u7528\u6a19\u6e96\u8a66\u6599\u3068\u54c1\u8cea\u7ba1\u7406\uff08QC\uff09\u7528\u6a19\u6e96\u8a66\u6599\u306e\u8abf\u88fd<\/h3>\n\n\n\n

\u3010\u6821\u6b63\u7528\u6a19\u6e96\u6eb6\u6db2\u3011
10,000, 5000, 2500, 1000, 500, 100, 50, 20 ng\/mL\u306e\u6fc3\u5ea6\u306b\u306a\u308b\u3088\u3046\u306b\u300110,000 ng\/mL\u306e\u6a19\u6e96\u6eb6\u6db2\u3092\u30e1\u30bf\u30ce\u30fc\u30eb\u3067\u5e0c\u91c8\u3057\u3001\u8abf\u88fd
\u3010\u54c1\u8cea\u7ba1\u7406\uff08QC\uff09\u7528\u6a19\u6e96\u6eb6\u6db2\u3011
50, 650, 1500, 6500 ng\/mL\u306e\u6fc3\u5ea6\u306b\u306a\u308b\u3088\u3046\u306b\u300110,000 ng\/mL\u306e\u6a19\u6e96\u6eb6\u6db2\u3092\u30e1\u30bf\u30ce\u30fc\u30eb\u3067\u5e0c\u91c8\u3057\u3001\u8abf\u88fd
\u3010\u5185\u90e8\u6a19\u6e96\u7269\u8cea\u3011
Table I<\/strong>\u306b\u793a\u3057\u305f\u5206\u6790\u6210\u5206\u3092\u4f7f\u7528\u3057\u3066\u3001100 ng\/mL\u306e\u6fc3\u5ea6\u306b\u306a\u308b\u3088\u3046\u306b\u8abf\u88fd<\/p>\n\n\n\n

\u4e0a\u8a18\u306e\u65b9\u6cd5\u3067\u8abf\u88fd\u3057\u305f\u5404\u6a19\u6e96\u6eb6\u6db2100 \u00b5L\u3092\u5408\u6210\u53e3\u8154\u6db2\uff08UTAK\u793e\u88fd\uff09900 \u00b5L\u306b\u52a0\u3048\u306610\u500d\u306b\u5e0c\u91c8\u3057\u3001\u6821\u6b63\u7528\u304a\u3088\u3073QC\u7528\u306e\u6a19\u6e96\u8a66\u6599\u3092\u4f5c\u88fd\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u5f8c\u3001\u4e0b\u8a18\u306e\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u3092\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

Table I:<\/strong> \u5185\u90e8\u6a19\u6e96\u7269\u8cea\u304a\u3088\u3073MRM\u30c8\u30e9\u30f3\u30b8\u30b7\u30e7\u30f3<\/p>\n\n\n\n

\u5185\u90e8\u6a19\u6e96\u7269\u8cea<\/strong><\/th>Precursor Ion<\/strong><\/th>Product Ion 1<\/strong><\/th><\/tr><\/thead>
Methamphetamine-D5<\/td>155.6<\/td>92.1<\/td><\/tr>
Norfentanyl-D5<\/td>238.6<\/td>55.2<\/td><\/tr>
Oxazepam-D5<\/td>292.3<\/td>246.1<\/td><\/tr>
LSD-D3<\/td>327.2<\/td>179.9<\/td><\/tr>
MDMA-D5<\/td>199.1<\/td>107.1<\/td><\/tr>
Ketamine-D4<\/td>242.5<\/td>129.1<\/td><\/tr>
7-Aminoclonazepam-D4<\/td>290.4<\/td>121.0<\/td><\/tr>
6-Acetylmorphine-D3<\/td>331.3<\/td>165.0<\/td><\/tr>
PCP-D5<\/td>249.4<\/td>96.2<\/td><\/tr>
Fentanyl-D5<\/td>342.2<\/td>105.0<\/td><\/tr>
EDDP-D3<\/td>280.7<\/td>234.2<\/td><\/tr>
Hydromorphone-D3<\/td>289.1<\/td>184.9<\/td><\/tr>
Methadone-D3<\/td>313.1<\/td>105.1<\/td><\/tr>
Buprenorphine-D4<\/td>471.9<\/td>243.2<\/td><\/tr>
Temazepam-D5<\/td>306.3<\/td>260.0<\/td><\/tr>
Norbuprenorphine-D4<\/td>417.6<\/td>186.9<\/td><\/tr>
Morphine-D3<\/td>289.6<\/td>164.9<\/td><\/tr>
Oxycodone-D6<\/td>322.4<\/td>247.1<\/td><\/tr>
Oxymorphone-D3<\/td>305.4<\/td>230.1<\/td><\/tr>
Nordiazepam-D5<\/td>276.3<\/td>140.1<\/td><\/tr>
Alprazolam-D5<\/td>314.2<\/td>286.0<\/td><\/tr>
Isotonitazene-D7<\/td>418.2<\/td>100.0<\/td><\/tr>
Cocaine-D3<\/td>307.3<\/td>185.3<\/td><\/tr>
Lidocaine-D10<\/td>245.2<\/td>64.0<\/td><\/tr>
delta-9-THC-D3<\/td>318.2<\/td>193.1<\/td><\/tr>
Gabapentin-D10<\/td>182.0<\/td>147.2<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u6cd5<\/h3>\n\n\n\n

1. \u5e0c\u91c8\u6ce8\u5165\u6cd5<\/h4>\n\n\n\n

\u25e6\u3000\u5b9f\u969b\u306e\u6fc3\u5ea6\u306b\u8fd1\u3044\u6fc3\u5ea6\u3067\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u884c\u3046\u305f\u3081\u306b\u6821\u6b63\u7528\u6a19\u6e96\u8a66\u6599\u304a\u3088\u3073QC\u7528\u6a19\u6e96\u8a66\u6599\u3001\u54041 mL\u306bQuantisal\u7de9\u885d\u6db2\uff08Immunalysis\u793e\u88fd\uff093 mL\u3092\u6dfb\u52a0
\u25e6\u3000\u3053\u306e\u7de9\u885d\u6db2\u6dfb\u52a0\u6e08\u307f\u306e\u6a19\u6e96\u8a66\u6599\u305d\u308c\u305e\u308c100 \u00b5L\u30922 mL\u5bb9\u91cf\u306e\u5fae\u91cf\u9060\u5fc3\u30c1\u30e5\u30fc\u30d6\u306b\u5165\u308c\u305f\u5f8c\u3001\u5185\u90e8\u6a19\u6e96\u7269\u8cea20 \u00b5L\u3092\u6dfb\u52a0\u3057\u300110\u79d2\u9593\u64b9\u62cc\uff08\u4ee5\u964d\u3053\u306e\u72b6\u614b\u3092\u30b5\u30f3\u30d7\u30eb\u3068\u3059\u308b\uff09
\u25e6\u3000\u5404\u30b5\u30f3\u30d7\u30eb\u306b\u30010.1%\u30ae\u9178\u6c34\u6eb6\u6db2:0.1%\u30ae\u9178\u30e1\u30bf\u30ce\u30fc\u30eb\u6eb6\u6db2\u309290:10\u3067\u8abf\u88fd\u3057\u305f\u6eb6\u6db2460 \u00b5L\u3092\u6dfb\u52a0\u3057\u3001\u518d\u5ea6\u64b9\u62cc
\u25e6\u3000\u30a4\u30f3\u30b5\u30fc\u30c8\u4ed8\u304d\u306eLC\u30d0\u30a4\u30a2\u30eb\uff082.0 mL\u8910\u8272\u30b7\u30e7\u30fc\u30c8\u30ad\u30e3\u30c3\u30d7\u30d0\u30a4\u30a2\u30eb\uff1acat.# 21142\u300150 \u00b5L\u30a4\u30f3\u30b5\u30fc\u30c8\uff1acat.# 24513\uff09\u306b\u8a66\u6599\u3092\u79fb\u3057\u30019 mm\u30b7\u30e7\u30fc\u30c8\u30b9\u30af\u30ea\u30e5\u30fc\u30ad\u30e3\u30c3\u30d7\uff08cat.# 24497\uff09\u3067\u5bc6\u5c01\u5f8c\u3001LC-MS\/MS\u3067\u5206\u6790\u3057\u305f\u3002<\/p>\n\n\n\n

2. \u4fdd\u6301\u578b\u6db2\u6db2\u62bd\u51fa\uff08SLE\uff09<\/h4>\n\n\n\n

\u25e6\u3000\u5b9f\u969b\u306e\u6fc3\u5ea6\u306b\u8fd1\u3044\u6fc3\u5ea6\u3067\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u884c\u3046\u305f\u3081\u306b\u6821\u6b63\u7528\u6a19\u6e96\u8a66\u6599\u304a\u3088\u3073QC\u7528\u6a19\u6e96\u8a66\u6599\u3001\u54041 mL\u306bQuantisal\u7de9\u885d\u6db2\uff08Immunalysis\u793e\u88fd\uff093 mL\u3092\u6dfb\u52a0
\u25e6\u3000\u3053\u306e\u7de9\u885d\u6db2\u6dfb\u52a0\u6e08\u307f\u306e\u6a19\u6e96\u8a66\u6599\u305d\u308c\u305e\u308c100 \u00b5L\u30922 mL\u5bb9\u91cf\u306e\u5fae\u91cf\u9060\u5fc3\u30c1\u30e5\u30fc\u30d6\u306b\u5165\u308c\u305f\u5f8c\u30015%\u6c34\u9178\u5316\u30a2\u30f3\u30e2\u30cb\u30a6\u30e0100 \u00b5L\u3068\u5185\u90e8\u6a19\u6e96\u7269\u8cea20 \u00b5L\u3092\u6dfb\u52a0\u3057\u3001\u64b9\u62cc\uff08\u4ee5\u964d\u3053\u306e\u72b6\u614b\u3092\u30b5\u30f3\u30d7\u30eb\u3068\u3059\u308b\uff09
\u25e6\u3000\u5404\u30b5\u30f3\u30d7\u30eb200 \u00b5L\u3092200 mg\u5bb9\u91cf\u306eResprep SLE\u30ab\u30fc\u30c8\u30ea\u30c3\u30b8(cat.# 28302)\u306b\u5145\u586b\uff08\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u306a\u5206\u6790\u306b\u306f\u3001Resprep SLE96\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\uff08cat.# 28304\uff09\u304c\u4f7f\u7528\u53ef\u80fd\uff09
\u25e6\u3000\u5145\u586b\u5264\u306b\u5404\u30b5\u30f3\u30d7\u30eb\u304c\u4fdd\u6301\u3055\u308c\u308b\u3088\u3046\u306b\u3001\u6700\u521d\u306e\u6570\u79d2\u9593\u306f\u8efd\u3044\u771f\u7a7a\u72b6\u614b\u306b\u3057\u3001\u305d\u306e\u5f8c\u306e5\u5206\u9593\u3001\u5404\u30b5\u30f3\u30d7\u30eb\u3092\u5145\u586b\u5264\u306b\u4fdd\u6301\u3055\u305b\u305f\u5f8c\u3001\u30b8\u30af\u30ed\u30ed\u30e1\u30bf\u30f3:\u30a4\u30bd\u30d7\u30ed\u30d1\u30ce\u30fc\u30eb\u309295:5\u3067\u8abf\u88fd\u3057\u305f\u6eb6\u6db2500 \u00b5L\u306b\u305d\u308c\u305e\u308c\u6eb6\u89e3\u3055\u305b\u308b
\u25e6\u3000\u5404\u30b5\u30f3\u30d7\u30eb\u3092\u84b8\u767a\u4e7e\u56fa\u3057\u305f\u5f8c\u30010.1%\u30ae\u9178\u6c34\u6eb6\u6db2:0.1%\u30ae\u9178\u30e1\u30bf\u30ce\u30fc\u30eb\u6eb6\u6db2\u309290:10\u3067\u8abf\u88fd\u3057\u305f\u6eb6\u6db2100 \u00b5L\u306b\u518d\u6eb6\u89e3\u3055\u305b\u3001\u64b9\u62cc
\u25e6\u3000\u30a4\u30f3\u30b5\u30fc\u30c8\u4ed8\u304d\u306eLC\u30d0\u30a4\u30a2\u30eb\uff082.0 mL\u8910\u8272\u30b7\u30e7\u30fc\u30c8\u30ad\u30e3\u30c3\u30d7\u30d0\u30a4\u30a2\u30eb\uff1acat.# 21142\u300150 \u00b5L\u30a4\u30f3\u30b5\u30fc\u30c8\uff1acat.# 24513\uff09\u306b\u8a66\u6599\u3092\u79fb\u3057\u30019 mm\u30b7\u30e7\u30fc\u30c8\u30b9\u30af\u30ea\u30e5\u30fc\u30ad\u30e3\u30c3\u30d7\uff08cat.# 24497\uff09\u3067\u5bc6\u5c01\u5f8c\u3001LC-MS\/MS\u3067\u5206\u6790\u3057\u305f\u3002<\/p>\n\n\n\n

3. \u5869\u6790\u652f\u63f4\u6db2\u6db2\u62bd\u51fa\uff08SALLE\uff09<\/h4>\n\n\n\n

\u25e6\u3000\u5b9f\u969b\u306e\u6fc3\u5ea6\u306b\u8fd1\u3044\u6fc3\u5ea6\u3067\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u3092\u884c\u3046\u305f\u3081\u306b\u6821\u6b63\u7528\u6a19\u6e96\u8a66\u6599\u304a\u3088\u3073QC\u7528\u6a19\u6e96\u8a66\u6599\u3001\u54041 mL\u306bQuantisal\u7de9\u885d\u6db2\uff08Immunalysis\u793e\u88fd\uff093 mL\u3092\u6dfb\u52a0
\u25e6\u3000\u3053\u306e\u7de9\u885d\u6db2\u6dfb\u52a0\u6e08\u307f\u306e\u6a19\u6e96\u8a66\u6599\u305d\u308c\u305e\u308c100 \u00b5L\u30922 mL\u5bb9\u91cf\u306e\u5fae\u91cf\u9060\u5fc3\u30c1\u30e5\u30fc\u30d6\u306b\u5165\u308c\u305f\u5f8c\u3001\u5185\u90e8\u6a19\u6e96\u7269\u8cea20 \u00b5L\u3092\u6dfb\u52a0\u3057\u300110\u79d2\u9593\u64b9\u62cc\uff08\u4ee5\u964d\u3053\u306e\u72b6\u614b\u3092\u30b5\u30f3\u30d7\u30eb\u3068\u3059\u308b\uff09
\u25e6\u3000\u5404\u30b5\u30f3\u30d7\u30eb\u304c\u5165\u3063\u3066\u3044\u308b\u30c1\u30e5\u30fc\u30d6\u306b\u3001\u98fd\u548cNaCl\u6eb6\u6db2100 \u00b5L\u3092\u5165\u308c\u305f\u5f8c\u3001\u30a2\u30bb\u30c8\u30cb\u30c8\u30ea\u30eb280 \u00b5L\u3092\u6dfb\u52a0\u3057\u3001\u518d\u5ea610\u79d2\u9593\u64b9\u62cc\u5f8c\u30013700 rpm\u306710\u5206\u9593\u9060\u5fc3\u5206\u96e2\u3092\u5b9f\u65bd
\u25e6\u3000\u6709\u6a5f\u5c64200 \u00b5L\u3092\u8a66\u9a13\u7ba1\u306b\u6c34\u6eb6\u6db2\u3068\u3057\u3066\u63a1\u53d6\u3057\u3001\u7a92\u7d20\u4e0b\u3067\u84b8\u767a\u4e7e\u56fa
\u25e6\u3000\u5404\u30b5\u30f3\u30d7\u30eb\u306b\u30010.1%\u30ae\u9178\u6c34\u6eb6\u6db2:0.1%\u30ae\u9178\u30e1\u30bf\u30ce\u30fc\u30eb\u6eb6\u6db2\u309290:10\u3067\u8abf\u88fd\u3057\u305f\u6eb6\u6db250 \u00b5L\u3092\u6dfb\u52a0\u3057\u3001\u518d\u5ea6\u64b9\u62cc
\u25e6\u3000\u30a4\u30f3\u30b5\u30fc\u30c8\u4ed8\u304d\u306eLC\u30d0\u30a4\u30a2\u30eb\uff082.0 mL\u8910\u8272\u30b7\u30e7\u30fc\u30c8\u30ad\u30e3\u30c3\u30d7\u30d0\u30a4\u30a2\u30eb\uff1acat.# 21142\u300150 \u00b5L\u30a4\u30f3\u30b5\u30fc\u30c8\uff1acat.# 24513\uff09\u306b\u8a66\u6599\u3092\u79fb\u3057\u30019 mm\u30b7\u30e7\u30fc\u30c8\u30b9\u30af\u30ea\u30e5\u30fc\u30ad\u30e3\u30c3\u30d7\uff08cat.# 24497\uff09\u3067\u5bc6\u5c01\u5f8c\u3001LC-MS\/MS\u3067\u5206\u6790\u3057\u305f\u3002<\/p>\n\n\n\n

\u30b5\u30f3\u30d7\u30eb\u63a1\u53d6\u30ad\u30c3\u30c8\u304b\u3089\u306e\u56de\u53ce\u91cf\u691c\u8a3c<\/h3>\n\n\n\n

\u5b9f\u969b\u306bQuantisal\u30ad\u30c3\u30c8\u3092\u4f7f\u7528\u3057\u305f\u969b\u306e\u3001\u30b5\u30f3\u30d7\u30eb\u56de\u53ce\u91cf\u3092\u78ba\u8a8d\u3059\u308b\u30c6\u30b9\u30c8\u3092\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002\u307e\u305a\u3001\u30ad\u30c3\u30c8\u5185\u306e\u30b9\u30dd\u30f3\u30b8\u3092\u3001\u5408\u6210\u53e3\u8154\u6db2\u306e\u30d6\u30e9\u30f3\u30af\u30b5\u30f3\u30d7\u30eb1 mL\uff08150 ng\/mL\u306e\u6fc3\u5ea6\u306b\u306a\u308b\u3088\u3046\u306b\u6dfb\u52a0\u6e08\u307f\uff09\u306b\u6d78\u3057\u307e\u3057\u305f\u3002\u6b21\u306b\u3001\u3053\u306e1 mL\u3092\u3059\u3079\u3066\u5438\u53ce\u3057\u305f\u30b9\u30dd\u30f3\u30b8\u3092\u7de9\u885d\u6db2\u306b\u6d78\u3057\u3001\u51b7\u8535\u5eab\u3067\u4e00\u6669\u4fdd\u7ba1\u3057\u3001\u305d\u306e\u5f8c\u3001SALLE\u3068\u540c\u69d8\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3067\u51e6\u7406\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u3088\u3046\u306b\u3057\u3066\u3001\u30b9\u30dd\u30f3\u30b8\u304b\u3089\u306e\u30b5\u30f3\u30d7\u30eb\u56de\u53ce\u65b9\u6cd5\uff08\u624b\u52d5\u5727\u643e vs. \u9060\u5fc3\u5206\u96e2\uff09\u306b\u3088\u308b\u56de\u53ce\u91cf\u3084\u5206\u6790\u6210\u5206\u306e\u56de\u53ce\u7387\u3078\u306e\u5f71\u97ff\u3082\u691c\u8a3c\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u5206\u6790\u6761\u4ef6<\/h3>\n\n\n\n

\u53e3\u8154\u6db2\u30b5\u30f3\u30d7\u30eb\u3092\u7528\u3044\u305f\u4e71\u7528\u85ac\u7269\u5206\u6790\u3092\u4ee5\u4e0b\u306e\u6761\u4ef6\u3067\u884c\u3044\u307e\u3057\u305f\u3002\u5404\u5316\u5408\u7269\u306e\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3055\u308c\u305f\u30a4\u30aa\u30f3\u306fTable II<\/strong>\u3092\u53c2\u7167\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u5206\u6790\u88c5\u7f6e : \u3000\u5cf6\u6d25 Nexera X2 LC\u3000\uff08\u691c\u51fa\u5668\uff1aSCIEX 4500 MS\/MS\uff09<\/p>\n\n\n\n

\u5206\u6790\u30ab\u30e9\u30e0 : \u00a0 \u00a0Raptor Biphenyl 2.7 \u00b5m, 50 mm x 2.1 mm (cat.# 9309A52)<\/p>\n\n\n\n

\u30ac\u30fc\u30c9\u30ab\u30e9\u30e0: \u00a0 \u00a0Raptor Biphenyl EXP \u30ac\u30fc\u30c9\u30ab\u30e9\u30e0\u30ab\u30fc\u30c8\u30ea\u30c3\u30b8 5 x 2.1 mm, 2.7 \u00b5m (cat # 9309A0252)<\/p>\n\n\n\n

\u79fb\u52d5\u76f8A:    0.1% \u30ae\u9178\u6c34\u6eb6\u6db2<\/p>\n\n\n\n

\u79fb\u52d5\u76f8B:    0.1% \u30ae\u9178\u30e1\u30bf\u30ce\u30fc\u30eb\u6eb6\u6db2<\/p>\n\n\n\n

\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\uff1a15% B (0\u5206) \u2192 20% B (1.00\u5206)  \u2192 20% B (2.00\u5206) \u2192 50% B (4.00\u5206)\u2192 60% B (6.00\u5206)\u2192 100% B (8.00\u5206) \u2192 100% B (9.00\u5206)  \u2192  15% B (9.01\u5206)  \u2192  15% B (10.00\u5206)<\/p>\n\n\n\n

\u6d41\u901f:    0.5 mL\/min<\/p>\n\n\n\n

\u6ce8\u5165\u91cf:    5 \u00b5L<\/p>\n\n\n\n

\u30ab\u30e9\u30e0\u6e29\u5ea6:    40 \u00b0C<\/p>\n\n\n\n

\u30a4\u30aa\u30f3\u5316\u30e2\u30fc\u30c9:    Positive ESI<\/p>\n\n\n\n

Table II:<\/strong> \u30bf\u30fc\u30b2\u30c3\u30c8\u4e71\u7528\u85ac\u7269\u3068LC-MS\/MS\u5206\u6790\u3067\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3055\u308c\u305f\u5404\u30a4\u30aa\u30f3<\/p>\n\n\n\n

\u30bf\u30fc\u30b2\u30c3\u30c8\u6210\u5206<\/strong><\/th>tR<\/strong><\/th>Precursor Ion<\/strong><\/th>Product Ion 1<\/strong><\/th>Product Ion 2<\/strong><\/th><\/tr><\/thead>
Morphine<\/td>0.82<\/td>286.10<\/td>152.10<\/td>165.00<\/td><\/tr>
Pregabalin<\/td>0.88<\/td>160.18<\/td>55<\/td>97.1<\/td><\/tr>
Oxymorphone<\/td>0.92<\/td>302.14<\/td>227.20<\/td>198.20<\/td><\/tr>
Cathinone<\/td>0.94<\/td>149.90<\/td>132.90<\/td>106.20<\/td><\/tr>
Amphetamine<\/td>1.18<\/td>136.22<\/td>91.00<\/td>65.10<\/td><\/tr>
Hydromorphone<\/td>1.22<\/td>286.16<\/td>184.90<\/td>156.90<\/td><\/tr>
Gabapentin<\/td>1.22<\/td>172.20<\/td>154.00<\/td>137.10<\/td><\/tr>
Methcathinone<\/td>1.33<\/td>164.50<\/td>146.15<\/td>131.20<\/td><\/tr>
MDA<\/td>1.50<\/td>180.13<\/td>163.10<\/td>105.20<\/td><\/tr>
Methamphetamine<\/td>1.53<\/td>150.25<\/td>91.10<\/td>119.00<\/td><\/tr>
Phentermine<\/td>1.78<\/td>150.24<\/td>91.10<\/td>133.10<\/td><\/tr>
Methylone<\/td>1.80<\/td>207.90<\/td>159.90<\/td>189.90<\/td><\/tr>
Lidocaine<\/td>1.83<\/td>235.40<\/td>86.00<\/td>58.20<\/td><\/tr>
Naloxone<\/td>1.90<\/td>328.27<\/td>310.10<\/td>212.10<\/td><\/tr>
Dihydrocodeine<\/td>2.09<\/td>302.20<\/td>199.00<\/td>128.20<\/td><\/tr>
MDMA<\/td>2.15<\/td>194.17<\/td>163.00<\/td>105.1<\/td><\/tr>
Codeine<\/td>2.22<\/td>300.16<\/td>152.00<\/td>165.10<\/td><\/tr>
6-Acetylmorphine<\/td>2.38<\/td>328.30<\/td>164.90<\/td>211.10<\/td><\/tr>
Levamisole<\/td>2.59<\/td>205.08<\/td>178.00<\/td>91.10<\/td><\/tr>
Oxycodone<\/td>2.69<\/td>316.19<\/td>298.00<\/td>169.00<\/td><\/tr>
Naltrexone<\/td>2.85<\/td>342.19<\/td>324.00<\/td>267.00<\/td><\/tr>
MDEA<\/td>2.92<\/td>208.13<\/td>163.00<\/td>105.20<\/td><\/tr>
Hydrocodone<\/td>2.98<\/td>300.14<\/td>199.00<\/td>128.00<\/td><\/tr>
Norketamine<\/td>3.30<\/td>224.08<\/td>125.00<\/td>89.10<\/td><\/tr>
Eutylone<\/td>3.39<\/td>236.10<\/td>188.05<\/td>218.15<\/td><\/tr>
Norfentanyl<\/td>3.55<\/td>233.13<\/td>84.10<\/td>55.00<\/td><\/tr>
4-Hydroxy nitazene<\/td>3.70<\/td>369.10<\/td>100.20<\/td>72.20<\/td><\/tr>
Pentylone<\/td>3.73<\/td>236.10<\/td>188.05<\/td>218.15<\/td><\/tr>
Dextrorphan<\/td>3.78<\/td>258.15<\/td>157.00<\/td>201.20<\/td><\/tr>
Xylazine<\/td>3.85<\/td>221.90<\/td>90.10<\/td>71.90<\/td><\/tr>
Ketamine<\/td>3.90<\/td>238.07<\/td>125.10<\/td>89.10<\/td><\/tr>
Benzoylecgonine<\/td>4.00<\/td>290.13<\/td>168.10<\/td>77.10<\/td><\/tr>
Meperidine<\/td>4.03<\/td>248.14<\/td>220.10<\/td>174.10<\/td><\/tr>
7-Aminoclonazepam<\/td>4.05<\/td>286.08<\/td>121.20<\/td>250.10<\/td><\/tr>
Cocaine<\/td>4.28<\/td>304.18<\/td>182.00<\/td>77.10<\/td><\/tr>
7-Hydroxymitragynine<\/td>4.50<\/td>415.60<\/td>189.90<\/td>175.00<\/td><\/tr>
Cocaethylene<\/td>4.60<\/td>318.21<\/td>196.10<\/td>82.00<\/td><\/tr>
LSD<\/td>4.60<\/td>324.27<\/td>223.10<\/td>208.00<\/td><\/tr>
Norbuprenorphine<\/td>4.65<\/td>414.30<\/td>250.10<\/td>187.10<\/td><\/tr>
Chlordiazepoxide<\/td>4.74<\/td>300.10<\/td>282.00<\/td>227.00<\/td><\/tr>
Acetyl fentanyl<\/td>4.75<\/td>323.28<\/td>188.00<\/td>105.00<\/td><\/tr>
Zolpidem<\/td>4.80<\/td>308.21<\/td>235.20<\/td>218.90<\/td><\/tr>
Fentanyl<\/td>5.15<\/td>337.29<\/td>188.00<\/td>105.10<\/td><\/tr>
Dextromethorphan<\/td>5.17<\/td>272.19<\/td>215.10<\/td>170.90<\/td><\/tr>
Isotonitazene<\/td>5.18<\/td>411.00<\/td>100.20<\/td>72.15<\/td><\/tr>
PCP<\/td>5.20<\/td>244.15<\/td>86.10<\/td>159.10<\/td><\/tr>
Buprenorphine<\/td>5.20<\/td>468.30<\/td>396.20<\/td>414.20<\/td><\/tr>
Midazolam<\/td>5.22<\/td>326.19<\/td>291.10<\/td>248.90<\/td><\/tr>
Propoxyphene<\/td>5.33<\/td>340.27<\/td>265.90<\/td>58.10<\/td><\/tr>
Tianeptine<\/td>5.38<\/td>437.90<\/td>293.20<\/td>229.10<\/td><\/tr>
Protonitazene<\/td>5.49<\/td>411.00<\/td>100.30<\/td>72.15<\/td><\/tr>
Sufentanil<\/td>5.62<\/td>387.23<\/td>111.10<\/td>234.30<\/td><\/tr>
EDDP<\/td>5.62<\/td>278.15<\/td>234.30<\/td>249.20<\/td><\/tr>
Mitragynine<\/td>5.78<\/td>399.25<\/td>159.00<\/td>153.90<\/td><\/tr>
iso-Butonitazene<\/td>6.10<\/td>425.00<\/td>100.30<\/td>72.20<\/td><\/tr>
Methadone<\/td>6.25<\/td>310.20<\/td>264.90<\/td>105.10<\/td><\/tr>
Lorazepam<\/td>6.35<\/td>321.05<\/td>275.00<\/td>229.00<\/td><\/tr>
Oxazepam<\/td>6.40<\/td>287.07<\/td>268.80<\/td>241.20<\/td><\/tr>
Clonazepam<\/td>6.50<\/td>316.09<\/td>270.00<\/td>214.10<\/td><\/tr>
Nordiazepam<\/td>6.80<\/td>271.00<\/td>139.90<\/td>164.90<\/td><\/tr>
Clonazolam<\/td>6.83<\/td>354.10<\/td>308.10<\/td>279.80<\/td><\/tr>
Alprazolam<\/td>7.28<\/td>309.11<\/td>280.90<\/td>204.90<\/td><\/tr>
Temazepam<\/td>7.30<\/td>301.11<\/td>255.10<\/td>282.90<\/td><\/tr>
Bromazolam<\/td>7.40<\/td>353.10<\/td>325.20<\/td>218.00<\/td><\/tr>
Etizolam<\/td>7.52<\/td>343.10<\/td>314.00<\/td>258.90<\/td><\/tr>
Diazepam<\/td>7.59<\/td>285.07<\/td>153.90<\/td>192.90<\/td><\/tr>
Cannabidiol<\/td>7.85<\/td>315.20<\/td>193.00<\/td>123.20<\/td><\/tr>
Delta-9-THC<\/td>8.10<\/td>315.50<\/td>193.10<\/td>259.10<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u7d50\u679c\u3068\u8003\u5bdf<\/h2>\n\n\n\n

SLE\u306b\u304a\u3051\u308b\u6700\u9069\u306a\u6eb6\u51fa\u6eb6\u5a92\u306e\u9078\u5b9a<\/h3>\n\n\n\n

\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u306bSLE\u3092\u63a1\u7528\u3059\u308b\u30e1\u30bd\u30c3\u30c9\u3067\u306f\u3001\u5206\u6790\u6210\u5206\u306e\u6975\u6027\u306f\u591a\u5c90\u306b\u308f\u305f\u308a\u3001\u305d\u306e\u91cf\u3082\u591a\u304f\u306a\u308a\u307e\u3059\u3002\u3057\u305f\u304c\u3063\u3066\u3001\u9069\u5207\u306b\u5206\u6790\u6210\u5206\u3092\u6eb6\u51fa\u3055\u305b\u308b\u6eb6\u51fa\u6eb6\u5a92\u306e\u9078\u5b9a\u304c\u91cd\u8981\u306b\u306a\u3063\u3066\u304d\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u6700\u9069\u306a\u6eb6\u51fa\u6eb6\u5a92\u3092\u9078\u5b9a\u3059\u308b\u305f\u3081\u306b\u30017\u7a2e\u985e\u306e\u6eb6\u51fa\u6eb6\u5a92\u3092\u6bd4\u8f03\u3059\u308b\u30c6\u30b9\u30c8\u3092\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002\u30c6\u30b9\u30c8\u3057\u305f\u6eb6\u5a92\u306f\u3001\u30e1\u30bf\u30ce\u30fc\u30eb\u3001\u30a2\u30bb\u30c8\u30cb\u30c8\u30ea\u30eb\u3001\u30b8\u30af\u30ed\u30ed\u30e1\u30bf\u30f3\u3001\u30d8\u30ad\u30b5\u30f3\u300150:50 \u30b8\u30af\u30ed\u30ed\u30e1\u30bf\u30f3:\u9162\u9178\u30a8\u30c1\u30eb\u300195:5 \u30b8\u30af\u30ed\u30ed\u30e1\u30bf\u30f3:\u30a4\u30bd\u30d7\u30ed\u30d1\u30ce\u30fc\u30eb\u300195:5 \u30b8\u30af\u30ed\u30ed\u30e1\u30bf\u30f3:\u30e1\u30bf\u30ce\u30fc\u30eb\u3067\u3059\u3002\u4f4e\u6fc3\u5ea6\u3068\u9ad8\u6fc3\u5ea6\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u30c6\u30b9\u30c8\u3055\u308c\u3001\u3059\u3079\u3066\u306e\u5206\u6790\u6210\u5206\u306b\u3064\u3044\u3066\u30d4\u30fc\u30af\u5f62\u72b6\u3001\u30d4\u30fc\u30af\u9762\u7a4d\u3001\u30d4\u30fc\u30af\u9ad8\u3055\u3092\u6bd4\u8f03\u3057\u305f\u7d50\u679c\u300195:5 \u30b8\u30af\u30ed\u30ed\u30e1\u30bf\u30f3:\u30a4\u30bd\u30d7\u30ed\u30d1\u30ce\u30fc\u30eb\u304c\u6700\u3082\u591a\u304f\u306e\u5206\u6790\u6210\u5206\u3067\u826f\u597d\u306a\u30d4\u30fc\u30af\u5f62\u72b6\u3068\u611f\u5ea6\u3092\u793a\u3057\u305f\u305f\u3081\u3001\u6700\u9069\u306a\u6eb6\u5a92\u3068\u3057\u3066\u9078\u5b9a\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u6cd5\u306e\u6bd4\u8f03<\/h3>\n\n\n\n

\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u306bSLE\u304a\u3088\u3073SALLE\u3092\u63a1\u7528\u3057\u305f\u7d50\u679c\u3001\u5e0c\u91c8\u6ce8\u5165\u6cd5\u3068\u6bd4\u8f03\u3057\u3066\u3001\u307b\u3068\u3093\u3069\u306e\u5206\u6790\u6210\u5206\u3067\u30ec\u30b9\u30dd\u30f3\u30b9\u304c\u5927\u5e45\u306b\u5411\u4e0a\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n

SLE\u306f\u3001\u7279\u6027\u304c\u985e\u4f3c\u3059\u308b\u5206\u6790\u6210\u5206\u3092\u542b\u3080\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u7de9\u885d\u6db2\u3092\u9664\u53bb\u3059\u308b\u306e\u306b\u6709\u52b9\u3067\u3057\u305f\u3002\u4e00\u65b9\u3001SALLE\u306f\u3001\u3055\u307e\u3056\u307e\u306a\u7279\u6027\u3092\u6301\u3064\u85ac\u7269\u306b\u542b\u307e\u308c\u308b\u5316\u5408\u7269\u5168\u822c\u306b\u5bfe\u3057\u3066\u826f\u597d\u306a\u7d50\u679c\u3092\u793a\u3057\u307e\u3057\u305f\u3002\u672c\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u306b\u304a\u3051\u308bSLE\u306b\u3088\u308b\u524d\u51e6\u7406\u3067\u306f\u3001\u591a\u304f\u306e\u30aa\u30d4\u30a8\u30fc\u30c8\u3067\u30ec\u30b9\u30dd\u30f3\u30b9\u304c\u5411\u4e0a\u3057\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u30d7\u30ec\u30ac\u30d0\u30ea\u30f3\u3001\u30ac\u30d0\u30da\u30f3\u30c1\u30f3\u3001THC\u3001CBD\u306b\u3064\u3044\u3066\u306f\u30ec\u30b9\u30dd\u30f3\u30b9\u304c\u6975\u3081\u3066\u4f4e\u304f\u3001\u8a31\u5bb9\u3067\u304d\u308b\u6c34\u6e96\u306b\u306f\u9054\u3057\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u3057\u305f\u304c\u3063\u3066\u3001\u3053\u308c\u3089\u306e\u6210\u5206\u306b\u3064\u3044\u3066\u306fSLE\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3067\u306e\u7cbe\u5ea6\u304a\u3088\u3073\u518d\u73fe\u6027\u306e\u8a55\u4fa1\u3092\u884c\u3063\u3066\u3044\u307e\u305b\u3093\u3002<\/p>\n\n\n\n

Figure 1<\/strong>\u306b\u306f\u3001SLE\u3001SALLE\u3001\u5e0c\u91c8\u6ce8\u5165\u6cd5\u3068\u3044\u30463\u7a2e\u985e\u306e\u524d\u51e6\u7406\u3092\u884c\u3063\u305f\u5404\u30b5\u30f3\u30d7\u30eb\uff08\u6fc3\u5ea610 ng\/mL\uff09\u306e\u30d4\u30fc\u30af\u9762\u7a4d\u30ec\u30b9\u30dd\u30f3\u30b9\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n

Figure 1:<\/strong> SLE\u3001SALLE\u3001\u5e0c\u91c8\u6ce8\u5165\u6cd5\u3092\u884c\u3063\u305f\u6fc3\u5ea610 ng\/mL\u30b5\u30f3\u30d7\u30eb\u306e\u30d4\u30fc\u30af\u9762\u7a4d\u30ec\u30b9\u30dd\u30f3\u30b9\u6bd4\u8f03<\/div>
\n\n
\"\"<\/figure>\n\n\n\n

\u30a8\u30e9\u30fc\u30d0\u30fc\u306f\u6a19\u6e96\u504f\u5dee\u3092\u8868\u3057\u307e\u3059\u3002<\/em><\/p>\n\n<\/div><\/div><\/div>\n\n\n

SALLE\u3084SLE\u304c\u5e0c\u91c8\u6ce8\u5165\u6cd5\u3088\u308a\u3082\u512a\u308c\u305f\u30d1\u30d5\u30a9\u30fc\u30de\u30f3\u30b9\u3092\u793a\u3059\u306e\u306f\u3001\u30df\u30c8\u30e9\u30ae\u30cb\u30f3\u3068\u30ce\u30eb\u30d5\u30a7\u30f3\u30bf\u30cb\u30eb\u306e\u30d4\u30fc\u30af\u30ec\u30b9\u30dd\u30f3\u30b9\u3092\u6bd4\u8f03\u3057\u305f\u4ee5\u4e0b\u306e\u7d50\u679c\u304b\u3089\u3082\u5206\u304b\u308a\u307e\u3059\u3002\u30df\u30c8\u30e9\u30ae\u30cb\u30f3\u306fSALLE\u3092\u4f7f\u7528\u3057\u305f\u5834\u5408\u306b\u6700\u3082\u9ad8\u3044\u30ec\u30b9\u30dd\u30f3\u30b9\u3092\u793a\u3057 (Figure 2<\/strong>)\u3001\u30ce\u30eb\u30d5\u30a7\u30f3\u30bf\u30cb\u30eb\u306fSLE\u3092\u4f7f\u7528\u3057\u305f\u5834\u5408\u306b\u9ad8\u3044\u30ec\u30b9\u30dd\u30f3\u30b9\u3092\u793a\u3057\u307e\u3057\u305f (Figure 3<\/strong>)\u3002\u3053\u306e\u4f8b\u304c\u660e\u793a\u3057\u3066\u3044\u308b\u306e\u306f\u3001\u5206\u6790\u6210\u5206\u306b\u3088\u3063\u3066\u6700\u3082\u52b9\u679c\u7684\u306a\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u306f\u7570\u306a\u308b\u3001\u3068\u3044\u3046\u3053\u3068\u3067\u3059\u3002\u3069\u306e\u524d\u51e6\u7406\u6cd5\u3092\u9078\u629e\u3059\u308b\u304b\u6c7a\u5b9a\u3059\u308b\u969b\u306b\u306f\u3001\u5206\u6790\u6210\u5206\u30ea\u30b9\u30c8\u3068LOQ\u306e\u8981\u4ef6\u3092\u614e\u91cd\u306b\u8003\u616e\u3059\u3079\u304d\u3067\u3057\u3087\u3046\u3002<\/p>\n\n\n

Figure 2:<\/strong> \u30df\u30c8\u30e9\u30ae\u30cb\u30f3\u306b\u95a2\u3059\u308b\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u6cd5\u5225\u30ec\u30b9\u30dd\u30f3\u30b9\u6bd4\u8f03\u3000<\/div>
\n
\"Comparison<\/div>

LC_CF0842<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Conc.
(ng\/mL)<\/th>		Precursor Ion<\/th>Product Ion<\/th><\/tr><\/thead>\n
1.<\/td>Mitragynine<\/a><\/td>5.78<\/td>100<\/td>399.25<\/td>159.00<\/td><\/tr>\n<\/tbody><\/table><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n
Figure 3:<\/strong> \u30ce\u30eb\u30d5\u30a7\u30f3\u30bf\u30cb\u30eb\u306b\u95a2\u3059\u308b\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u6cd5\u5225\u30ec\u30b9\u30dd\u30f3\u30b9\u6bd4\u8f03<\/div>
\n
\"Comparison<\/div>

LC_CF0841<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Conc.
(ng\/mL)<\/th>		Precursor Ion<\/th>Product Ion<\/th><\/tr><\/thead>\n
1.<\/td>Norfentanyl<\/a><\/td>3.55<\/td>100<\/td>233.13<\/td>84.10<\/td><\/tr>\n<\/tbody><\/table><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n

\u30b5\u30f3\u30d7\u30eb\u63a1\u53d6\u30ad\u30c3\u30c8\u304b\u3089\u306e\u56de\u53ce\u91cf\u691c\u8a3c<\/h3>\n\n\n\n

\u53e3\u8154\u6db2\u63a1\u53d6\u30ad\u30c3\u30c8\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u3001\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u3068\u7de9\u885d\u6db2\u3092\u63a1\u53d6\u88c5\u7f6e\u5185\u306e\u30b9\u30dd\u30f3\u30b8\u304b\u3089\u9664\u53bb\u3059\u308b\u3053\u3068\u304c\u975e\u5e38\u306b\u91cd\u8981\u3067\u3059\u3002\u30b9\u30dd\u30f3\u30b8\u304b\u3089\u30b5\u30f3\u30d7\u30eb\u3092\u56de\u53ce\u3059\u308b\u4e3b\u306a\u65b9\u6cd5\u306f\u3001\u624b\u52d5\u5727\u643e\u3068\u9060\u5fc3\u5206\u96e2\u306e2\u3064\u3067\u3059\u3002Figure 4<\/strong>\u304b\u3089\u5206\u304b\u308b\u3088\u3046\u306b\u3001\u9060\u5fc3\u5206\u96e2\u3067\u306f4 mL\u5168\u91cf\u304c\u307b\u307c\u56de\u53ce\u3055\u308c\u305f\u306e\u306b\u5bfe\u3057\u3001\u624b\u52d5\u5727\u643e\u3067\u306f\u7d043 mL\u304c\u56de\u53ce\u3055\u308c\u305f\u306e\u307f\u3067\u3057\u305f\u3002<\/p>\n\n\n

Figure 4:<\/strong> \u53e3\u8154\u6db2\u30b5\u30f3\u30d7\u30eb\u3092\u63a1\u53d6\u88c5\u7f6e\u304b\u3089\u3088\u308a\u591a\u304f\u56de\u53ce\u3067\u304d\u305f\u306e\u306f\u9060\u5fc3\u5206\u96e2\uff08\u5de6\uff09\u3067\u3057\u305f\u3002\uff08\u53f3\uff09\u306f\u624b\u52d5\u5727\u643e\u3002<\/div>
\n\n
\"\"<\/figure>\n\n<\/div><\/div><\/div>\n\n\n

\u30b9\u30dd\u30f3\u30b8\u304b\u3089\u3059\u3079\u3066\u306e\u53e3\u8154\u6db2\u3068\u7de9\u885d\u6db2\u3092\u62bd\u51fa\u3059\u308b\u306b\u306f\u3001\u9060\u5fc3\u5206\u96e2\u304c\u3088\u308a\u52b9\u679c\u7684\u3067\u3042\u308b\u3053\u3068\u306f\u660e\u3089\u304b\u3067\u3059\u3002\u305f\u3060\u3057\u3001\u7de9\u885d\u6db2\u306b\u6d78\u3055\u308c\u305f\u30b9\u30dd\u30f3\u30b8\u306f\u3082\u308d\u304f\u306a\u3063\u3066\u304a\u308a\u3001\u9ad8\u901f\u3067\u9060\u5fc3\u5206\u96e2\u3059\u308b\u3068\u30a2\u30d7\u30ea\u30b1\u30fc\u30bf\u30fc\u304b\u3089\u7c21\u5358\u306b\u5916\u308c\u3066\u3057\u307e\u3046\u306e\u3067\u3001\u9060\u5fc3\u5206\u96e2\u6642\u306b\u306f\u30b9\u30dd\u30f3\u30b8\u306e\u53d6\u308a\u6271\u3044\u306b\u6ce8\u610f\u304c\u5fc5\u8981\u3067\u3059\u3002\u9060\u5fc3\u5206\u96e2\u524d\u306b\u30b9\u30dd\u30f3\u30b8\u3092\u30c1\u30e5\u30fc\u30d6\u306e\u5074\u9762\u306b\u6298\u308a\u305f\u305f\u3080\u3053\u3068\u3092\u63a8\u5968\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n

\u30b5\u30f3\u30d7\u30eb\u56de\u53ce\u91cf\u304c\u3069\u306e\u7a0b\u5ea6\u5206\u6790\u6210\u5206\u306e\u56de\u53ce\u7387\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u304b\u3092\u78ba\u8a8d\u3059\u308b\u305f\u3081\u306b\u3001\u4e2d\u7a0b\u5ea6\u306eQC\u6fc3\u5ea6(150 ng\/mL)\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u624b\u52d5\u5727\u643e\u3068\u9060\u5fc3\u5206\u96e2\u3067\u56de\u53ce\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u30e1\u30bd\u30c3\u30c9\u306b\u542b\u307e\u308c\u308b68\u7a2e\u985e\u306e\u5206\u6790\u6210\u5206\u306e\u56de\u53ce\u7387\u306f\u3059\u3079\u3066\u3001\u9060\u5fc3\u5206\u96e2\u3092\u4f7f\u7528\u3057\u305f\u5834\u5408\u306e\u65b9\u304c\u826f\u597d\u3067\u3057\u305f\uff08Figure 5<\/strong>\u53c2\u7167\uff09\u3002<\/p>\n\n\n

Figure 5:<\/strong> \u624b\u52d5\u5727\u643e\u3068\u9060\u5fc3\u5206\u96e2\u306b\u3088\u3063\u3066\u56de\u53ce\u3057\u305f\u6fc3\u5ea610 ng\/mL\u30b5\u30f3\u30d7\u30eb\uff08\u30b9\u30dd\u30f3\u30b8\uff09\u306e\u56de\u53ce\u7387\u6bd4\u8f03<\/div>
\n\n
\"\"<\/figure>\n\n<\/div><\/div><\/div>\n\n\n

\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u6027\u80fd<\/h3>\n\n\n\n

Figure 6<\/strong>\u306b\u793a\u3059\u3088\u3046\u306b\u3001SALLE\u306b\u3088\u308b\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u3092\u63a1\u7528\u3057\u305f\u672c\u5206\u6790\u6761\u4ef6\u3067\u306f\u3001\u53e3\u8154\u6db2\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u65b0\u898f\u7cbe\u795e\u6d3b\u6027\u7269\u8cea\u3092\u542b\u3080\u516868\u7a2e\u985e\u306e\u4e71\u7528\u85ac\u7269\u3092\u300110\u5206\u306e\u30b5\u30a4\u30af\u30eb\u30bf\u30a4\u30e0\u3067\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3057\u305f\u3002\u5206\u6790\u306b\u306f\u3001LC-MS\/MS\u7528Raptor Biphenyl\u30ab\u30e9\u30e0\uff0850 \u00d7 2.1 mm\u30012.7 \u00b5m\uff09\u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u3053\u306e\u30e1\u30bd\u30c3\u30c9\u3067\u306f\u3001\u3059\u3079\u3066\u306e\u540c\u91cd\u4f53\u306e\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u5206\u96e2\u304c\u9069\u5207\u306b\u884c\u308f\u308c\u305f\u305f\u3081\u3001\u6b63\u78ba\u306a\u5b9a\u91cf\u304c\u53ef\u80fd\u3067\u3057\u305f\u3002<\/p>\n\n\n

Figure 6:<\/strong> Raptor Biphenyl LC\u30ab\u30e9\u30e0\u3092\u7528\u3044\u305f\u5408\u6210\u7d4c\u53e3\u6db2\u4e2d\u306e68\u7a2e\u985e\u306e\u4e71\u7528\u85ac\u7269\u306eLC-MS\/MS\u5206\u6790 (SALLE\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406)\uff08\u30d4\u30fc\u30af\u30ea\u30b9\u30c8\u3068\u5206\u6790\u6761\u4ef6\u3092\u542b\u3080\u5b8c\u5168\u7248\u306fPDF\u3067\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u53ef\uff09<\/div>
\n
\"Analysis<\/div>

LC_CF0830<\/p>

<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n

\u76f4\u7dda\u6027<\/h3>\n\n\n\n

\u7dda\u5f62\u56de\u5e30\uff081\/x \u91cd\u307f\u4ed8\u3051\uff09\u3092\u7528\u3044\u308b\u3068\u3001\u3059\u3079\u3066\u306e\u5206\u6790\u6210\u5206\u306f\u3001\u3044\u305a\u308c\u306e\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u6cd5\u306b\u304a\u3044\u3066\u30820.991\u4ee5\u4e0a\u306e\u8a31\u5bb9\u53ef\u80fd\u306aR\u00b2\u5024\u3092\u793a\u3057\u307e\u3057\u305f\u3002\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u4e71\u7528\u85ac\u7269\u306e\u5206\u6790\u7bc4\u56f2\u3092Table III<\/strong>\u306b\u793a\u3057\u307e\u3059\u3002<\/p>\n\n\n\n

Table III:<\/strong> \u53e3\u8154\u6db2\u306b\u542b\u307e\u308c\u308b\u5206\u6790\u6210\u5206\u306e\u5206\u6790\u7bc4\u56f2<\/p>\n\n\n\n

\u5206\u6790\u6210\u5206<\/strong><\/th>\u7dda\u5f62\u7bc4\u56f2 (ng\/mL)\u3000<\/strong><\/th>\u5206\u6790\u6210\u5206<\/strong><\/th>\u7dda\u5f62\u7bc4\u56f2 (ng\/mL)\u3000<\/strong><\/th><\/tr><\/thead>
Morphine<\/td>2-1000<\/td>Cocaine<\/td>2-1000<\/td><\/tr>
Pregabalin<\/td>50-10,000<\/td>7-hydroxymitragynine<\/td>10-1000<\/td><\/tr>
Oxymorphone<\/td>2-1000<\/td>LSD<\/td>2-1000<\/td><\/tr>
Cathinone<\/td>2-1000<\/td>Cocaethylene<\/td>2-1000<\/td><\/tr>
Amphetamine<\/td>2-1000<\/td>Norbuprenorphine<\/td>2-1000<\/td><\/tr>
Hydromorphone<\/td>2-1000<\/td>Chlordiazepoxide<\/td>2-1000<\/td><\/tr>
Gabapentin<\/td>50-10,000<\/td>Acetyl Fentanyl<\/td>2-1000<\/td><\/tr>
Methcathinone<\/td>5-1000<\/td>Zolpidem<\/td>2-1000<\/td><\/tr>
MDA<\/td>2-1000<\/td>Fentanyl<\/td>2-1000<\/td><\/tr>
Methamphetamine<\/td>2-1000<\/td>Dextromethorphan<\/td>2-1000<\/td><\/tr>
Phentermine<\/td>2-1000<\/td>Isotonitazene<\/td>2-1000<\/td><\/tr>
Methylone<\/td>2-1000<\/td>Buprenorphine<\/td>2-1000<\/td><\/tr>
Lidocaine<\/td>2-1000<\/td>PCP<\/td>2-1000<\/td><\/tr>
Naloxone<\/td>2-1000<\/td>Midazolam<\/td>2-1000<\/td><\/tr>
Dihydrocodeine<\/td>5-1000<\/td>Propoxyphene<\/td>2-1000<\/td><\/tr>
MDMA<\/td>2-1000<\/td>Tianeptine<\/td>2-1000<\/td><\/tr>
Codeine<\/td>2-1000<\/td>Protonitazene<\/td>2-1000<\/td><\/tr>
6-Acetylmorphine<\/td>2-1000<\/td>Sufentanil<\/td>2-1000<\/td><\/tr>
Levamisole<\/td>2-1000<\/td>EDDP<\/td>2-1000<\/td><\/tr>
Oxycodone<\/td>2-1000<\/td>Mitragynine<\/td>2-1000<\/td><\/tr>
Naltrexone<\/td>2-1000<\/td>Iso-Butonitazene<\/td>5-1000<\/td><\/tr>
MDEA<\/td>2-1000<\/td>Methadone<\/td>2-1000<\/td><\/tr>
Hydrocodone<\/td>2-1000<\/td>Lorazepam<\/td>2-1000<\/td><\/tr>
Norketamine<\/td>2-1000<\/td>Oxazepam<\/td>2-1000<\/td><\/tr>
Eutylone<\/td>2-1000<\/td>Clonazepam<\/td>2-1000<\/td><\/tr>
Norfentanyl<\/td>2-1000<\/td>Nordiazepam<\/td>5-1000<\/td><\/tr>
4-Hydroxy nitazene<\/td>2-1000<\/td>Clonazolam<\/td>2-1000<\/td><\/tr>
Pentylone<\/td>2-1000<\/td>Alprazolam<\/td>2-1000<\/td><\/tr>
Dextrorphan<\/td>5-1000<\/td>Temazepam<\/td>2-1000<\/td><\/tr>
Xylazine<\/td>5-1000<\/td>Bromazolam<\/td>2-1000<\/td><\/tr>
Ketamine<\/td>5-1000<\/td>Etizolam<\/td>2-1000<\/td><\/tr>
Benzoylecgonine<\/td>2-1000<\/td>Diazepam<\/td>2-1000<\/td><\/tr>
Meperidine<\/td>2-1000<\/td>Delta-9-THC<\/td>5-1000<\/td><\/tr>
7-Aminoclonazepam<\/td>5-1000<\/td>Cannabidiol<\/td>5-1000<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u7cbe\u5ea6\u3068\u518d\u73fe\u6027<\/h3>\n\n\n\n

\u7cbe\u5ea6\u3068\u518d\u73fe\u6027\u306f\u3001SLE\u304a\u3088\u3073SALLE\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u6cd5\u3092\u4f7f\u7528\u3057\u3066\u8907\u6570\u65e5\u306b\u308f\u305f\u3063\u3066\u78ba\u8a8d\u3055\u308c\u307e\u3057\u305f\u3002\u5b9a\u91cf\u9650\u754c\uff08LOQ\uff09\u3001\u5b9a\u91cf\u9650\u754c\uff08\u4f4e\uff09\u3001\u5b9a\u91cf\u9650\u754c\uff08\u4e2d\uff09\u3001\u304a\u3088\u3073\u5b9a\u91cf\u9650\u754c\uff08\u9ad8\uff09\u3067\u306e\u56de\u53ce\u7387\u304c\u671f\u5f85\u5024\u306e\u00b115%\u4ee5\u5185\u306b\u53ce\u307e\u308b\u3053\u3068\u3067\u3001\u4e21\u65b9\u306e\u524d\u51e6\u7406\u6cd5\u306b\u304a\u3044\u3066\u30e1\u30bd\u30c3\u30c9\u306e\u7cbe\u5ea6\u304c\u5b9f\u8a3c\u3055\u308c\u307e\u3057\u305f\u3002\u540c\u65e5\u5185\u304a\u3088\u3073\u5225\u65e5\u9593\u306e\u518d\u73fe\u6027\u3082\u3001%RSD\u304cSLE\u30679.12%\u4ee5\u4e0b\u3001SALLE\u30677.45%\u4ee5\u4e0b\u3068\u3001\u8a31\u5bb9\u7bc4\u56f2\u5185\u3067\u3057\u305f\u3002<\/p>\n\n\n\n

\u30ab\u30e9\u30e0\u5805\u7262\u6027<\/h3>\n\n\n\n

\u30ab\u30e9\u30e0\u306e\u5805\u7262\u6027\u306f\u3001SLE\u304a\u3088\u3073SALLE\u3092\u4f7f\u7528\u3057\u305f\u524d\u51e6\u7406\u6e08\u307f\u30b5\u30f3\u30d7\u30eb\u3092250\u56de\u4ee5\u4e0a\u9023\u7d9a\u3057\u3066\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u6ce8\u5165\u3057\u3066\u30c6\u30b9\u30c8\u3057\u307e\u3057\u305f\u3002Raptor Biphenyl\u30ab\u30e9\u30e0\u306f\u975e\u5e38\u306b\u5b89\u5b9a\u3057\u305f\u7d50\u679c\u3092\u793a\u3057\u300168\u7a2e\u985e\u306e\u4e71\u7528\u85ac\u7269\u3059\u3079\u3066\u306b\u304a\u3044\u3066\u3001\u6700\u521d\u3068\u6700\u5f8c\u306e\u6ce8\u5165\u306e\u4fdd\u6301\u6642\u9593\u306e\u5909\u52d5\u306f6.21%\u4ee5\u5185\u3067\u3057\u305f\u3002<\/p>\n\n\n\n

\u7d50\u8ad6<\/h2>\n\n\n\n

\u4eca\u56de\u306e\u5b9f\u9a13\u3067\u5b9f\u8a3c\u3057\u305fSALLE\u304a\u3088\u3073SLE\u3092\u63a1\u7528\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u6cd5\u306f\u3001\u5f93\u6765\u306eSPE\u306b\u6bd4\u3079\u3066\u5927\u5e45\u306a\u6642\u9593\u77ed\u7e2e\u3068\u4f5c\u696d\u52b9\u7387\u5316\u3092\u5b9f\u73fe\u3057\u307e\u3057\u305f\u3002\u307e\u305f\u3001LC-MS\/MS\u306b\u3088\u308b\u9ad8\u611f\u5ea6\u30fb\u9ad8\u7cbe\u5ea6\u306a\u53e3\u8154\u6db2\u4e2d\u306e\u85ac\u7269\u5206\u6790\uff0868\u7a2e\u985e\u306e\u4e71\u7528\u85ac\u7269\u304a\u3088\u3073\u65b0\u898f\u7cbe\u795e\u6d3b\u6027\u7269\u8cea\u306e\u5206\u6790\uff09\u3092\u53ef\u80fd\u306b\u3057\u307e\u3057\u305f\u3002SALLE\u306f\u591a\u69d8\u306a\u85ac\u7269\u306b\u3001SLE\u306f\u7279\u5b9a\u306e\u85ac\u7269\u30b0\u30eb\u30fc\u30d7\u306b\u9069\u3057\u3066\u304a\u308a\u3001\u76ee\u7684\u306b\u5fdc\u3058\u3066\u4f7f\u3044\u5206\u3051\u304c\u53ef\u80fd\u3067\u3059\u3002\u3069\u3061\u3089\u306e\u30b5\u30f3\u30d7\u30eb\u524d\u51e6\u7406\u6cd5\u3067\u3082\u3001\u5e0c\u91c8\u6ce8\u5165\u6cd5\u306b\u6bd4\u3079\u3066\u5206\u6790\u6210\u5206\u306e\u56de\u53ce\u7387\u304c\u5411\u4e0a\u3057\u3001\u7de9\u885d\u6db2\u9664\u53bb\u3082\u52b9\u679c\u7684\u306b\u884c\u308f\u308c\u305f\u305f\u3081\u3001\u30ab\u30e9\u30e0\u5bff\u547d\u304c\u9577\u304f\u306a\u308a\u3001\u4e0b\u6d41\u306b\u304a\u3051\u308b\u30e1\u30bd\u30c3\u30c9\u3078\u306e\u5f71\u97ff\u304c\u4f4e\u6e1b\u3067\u304d\u307e\u3057\u305f\u3002SALLE\u3068SLE\u306f\u3001\u3044\u305a\u308c\u3082\u30b7\u30f3\u30d7\u30eb\u306a\u624b\u9806\u3001\u77ed\u6642\u9593\u3067\u5b9f\u884c\u3067\u304d\u308b\u305f\u3081\u3001\u5f93\u6765\u306e\u3001\u6642\u9593\u304c\u304b\u304b\u308a\u8907\u96d1\u306a\u624b\u9806\u306eSPE\u3068\u6bd4\u3079\u3066\u30e1\u30ea\u30c3\u30c8\u304c\u3042\u308a\u307e\u3059\u3002\u4eca\u5f8c\u3001\u53e3\u8154\u6db2\u4e2d\u306e\u4e71\u7528\u85ac\u7269\u306e\u5206\u6790\u306b\u4f7f\u7528\u3055\u308c\u308b\u3053\u3068\u304c\u5897\u3048\u3066\u3044\u304f\u3067\u3057\u3087\u3046\u3002<\/p>\n\n\n\n

\u53c2\u8003\u6587\u732e<\/h2>\n\n\n\n
    \n
  1. E.J. Cone, M.A. Huestis, Marilyn, Interpretation of oral fluid tests for drugs of abuse, \u00a0Ann NY Acad Sci, \u00a0 \u00a01098 (2007) 51-103. https:\/\/doi.org\/10.1196\/annals.1384.037<\/a><\/li>\n\n\n\n
  2. S. Uljon, Chapter One-Advances in fentanyl testing, Advances in Clinical Chemistry, 116 (2023)1-30. https:\/\/doi.org\/10.1016\/bs.acc.2023.05.004<\/a><\/li>\n<\/ol>\n\n\n\n

    \u3053\u306e\u30e1\u30bd\u30c3\u30c9\u306f\u7814\u7a76\u7528\u9014\u306e\u307f\u306b\u958b\u767a\u3055\u308c\u3066\u304a\u308a\u3001\u8a3a\u65ad\u306b\u4f7f\u7528\u3059\u308b\u306b\u306f\u3055\u3089\u306a\u308b\u691c\u8a3c\u304c\u5fc5\u8981\u3067\u3059\u3002<\/em><\/p>\n\n\n\n

    <\/div>\n\n\n
    \n

    Products Mentioned<\/h3>\n
    \n
    \n
    \n
    \n Catalog No. 9309A52 <\/a> <\/div>\n
    \n
    Raptor Biphenyl, 2.7 \u00b5m, 50 x 2.1 mm HPLC\u30ab\u30e9\u30e0<\/div>\n <\/div>\n
    \n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 9309A0252 <\/a> <\/div>\n
    \n
    Raptor Biphenyl EXP Guard Column Cartridge, 2.7 \u00b5m, 5 x 2.1 mm, 3-pk.<\/div>\n <\/div>\n
    \n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 25808 <\/a> <\/div>\n
    \n
    EXP Direct Connect Holder for EXP Guard Cartridges, Includes Fitting & Ferrules<\/div>\n <\/div>\n
    \n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 28302 <\/a> <\/div>\n
    \n
    Resprep SLE Cartridges, 200 mg\/3 mL Cartridge, 50-pk.<\/div>\n <\/div>\n
    \n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 28304 <\/a> <\/div>\n
    \n
    Resprep SLE 96-Well Plates, 200 mg\/2 mL Each Well, ea.<\/div>\n <\/div>\n
    \n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 21142 <\/a> <\/div>\n
    \n
    Short-Cap Vial with Grad Marking Spot, 9-425 Screw-Thread, 2.0 mL, 9 mm, 12 x 32 (vial only), Amber, 100-pk.<\/div>\n <\/div>\n
    \n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 24513 <\/a> <\/div>\n
    \n
    Vial Inserts, Glass, Big Mouth w\/ Bottom Spring, 50 \u00b5L, 100-pk.<\/div>\n <\/div>\n
    \n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
    \n
    \n Catalog No. 24497 <\/a> <\/div>\n
    \n
    Short Screw Cap, Polypropylene, Screw-Thread, PTFE\/Silicone\/PTFE Septa, Blue, Preassembled, 2.0 mL, 9 mm, 100-pk.<\/div>\n <\/div>\n
    \n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n \n\n\n
    <\/div>\n","protected":false},"excerpt":{"rendered":"

    \u53e3\u8154\u6db2\u4e2d\u306e\u4e71\u7528\u85ac\u7269\u691c\u67fb\u306b\u304a\u3051\u308b\u56fa\u76f8\u62bd\u51fa\uff08SPE\uff09\u306e\u8ab2\u984c\u3092\u89e3\u6c7a\u3057\u307e\u3059\u3002SALLE\u3068SLE\u3092\u5e0c\u91c8\u6ce8\u5165\u6cd5\u3068\u6bd4\u8f03\u3057\u305f\u7d50\u679c\u3001\u5927\u5e45\u306a\u6642\u9593\u77ed\u7e2e\u3092\u5b9f\u73fe\u3057\u3064\u3064\u300168\u7a2e\u985e\u306e\u85ac\u7269\u3092\u9ad8\u7cbe\u5ea6\u3067\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3057\u305f\u3002\u7de9\u885d\u6db2\u306e\u30af\u30ea\u30fc\u30f3\u30a2\u30c3\u30d7\u3084\u30b5\u30f3\u30d7\u30eb\u56de\u53ce\u7387\u306b\u95a2\u3059\u308b\u5177\u4f53\u7684\u306a\u5b9f\u9a13\u7d50\u679c\u3068\u5171\u306b\u3001SALLE\u3068SLE\u306b\u3088\u3063\u3066\u85ac\u7269\u5206\u6790\u306e\u52b9\u7387\u5316\u304c\u56f3\u308c\u305f\u3053\u3068\u3092\u8a73\u8aac\u3057\u307e\u3059\u3002<\/p>\n","protected":false},"author":24,"featured_media":6427,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[785],"tags":[],"industries-application":[2277,2276,2266],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[2365],"hf_cat_post":[651],"ppma_author":[444],"class_list":["post-45333","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-application-notes-ja","industries-application-forensics-toxicology-ja","industries-application-forensics-ja","industries-application-clinical-ja","resource-technique-ms-ms-ja"],"acf":[],"taxonomy_info":{"category":[{"value":785,"label":"\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3"}],"industries-application":[{"value":2277,"label":"\u6cd5\u4e2d\u6bd2\u5b66"},{"value":2276,"label":"\u6cd5\u533b\u5b66"},{"value":2266,"label":"\u81e8\u5e8a"}],"resource-technique":[{"value":2365,"label":"MS\/MS"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-CFAN4466-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Samantha Herbick","author_link":"https:\/\/discover.restek.com\/ja\/author\/samantha-herbick\/"},"comment_info":0,"category_info":[{"term_id":785,"name":"\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3","slug":"application-notes-ja","term_group":0,"term_taxonomy_id":785,"taxonomy":"category","description":"","parent":0,"count":86,"filter":"raw","cat_ID":785,"category_count":86,"category_description":"","cat_name":"\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3","category_nicename":"application-notes-ja","category_parent":0}],"tag_info":false,"authors":[{"term_id":444,"user_id":24,"is_guest":0,"slug":"samantha-herbick","display_name":"Samantha Herbick","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-herbick-samantha.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-herbick-samantha.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/45333","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/users\/24"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/comments?post=45333"}],"version-history":[{"count":10,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/45333\/revisions"}],"predecessor-version":[{"id":81374,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/45333\/revisions\/81374"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media\/6427"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media?parent=45333"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/categories?post=45333"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/tags?post=45333"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/industries-application?post=45333"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/post-badge?post=45333"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-type?post=45333"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/product-library?post=45333"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-technique?post=45333"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/hf_cat_post?post=45333"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/ppma_author?post=45333"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}