{"id":48560,"date":"2025-04-15T00:00:00","date_gmt":"2025-04-15T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/inert-lc-columns-the-answer-to-poor-thc-sensitivity-japan\/"},"modified":"2026-01-21T18:48:26","modified_gmt":"2026-01-21T18:48:26","slug":"inert-lc-columns-the-answer-to-poor-thc-sensitivity-japan","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/blogs-ja\/gnbl4715-ja\/inert-lc-columns-the-answer-to-poor-thc-sensitivity-japan","title":{"rendered":"\u5168\u8840\u4e2d\u306eTHC\u9ad8\u611f\u5ea6\u5206\u6790\u3068\u30a4\u30ca\u30fc\u30c8LC\u30ab\u30e9\u30e0\u306e\u6709\u52b9\u6027"},"content":{"rendered":"\n

LC-MS\/MS\u306b\u3088\u308b\u5168\u8840\u4e2d\u306e\u30ab\u30f3\u30ca\u30d3\u30ce\u30a4\u30c9\u5206\u6790\u306f\u3001\u3055\u307e\u3056\u307e\u306a\u8981\u56e0\u306b\u3088\u308a\u975e\u5e38\u306b\u96e3\u6613\u5ea6\u304c\u9ad8\u304f\u3001\u306a\u304b\u3067\u3082\u5831\u544a\u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u3067\u6c42\u3081\u3089\u308c\u308b\u6975\u3081\u3066\u4f4e\u3044\u691c\u51fa\u9650\u754c\u3092\u9054\u6210\u3059\u308b\u3053\u3068\u304c\u6700\u5927\u306e\u8ab2\u984c\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u591a\u304f\u306e\u6cd5\u533b\u5b66\u7814\u7a76\u6240\u3067\u306f\u3001\u03949-\u30c6\u30c8\u30e9\u30d2\u30c9\u30ed\u30ab\u30f3\u30ca\u30d3\u30ce\u30fc\u30eb\uff08\u03949-THC\uff09\u3084\u300111-\u30d2\u30c9\u30ed\u30ad\u30b7-\u03949-THC\uff0811-OH-\u03949-THC\uff09\u300111-\u30ce\u30eb-9-\u30ab\u30eb\u30dc\u30ad\u30b7-\u03949-THC\uff08\u03949-THC-COOH\uff09\u3068\u3044\u3063\u305f\u91cd\u8981\u306a\u4ee3\u8b1d\u7269\u306b\u3064\u3044\u3066\u30010.5ng\/mL\u4ee5\u4e0b\u306e\u5b9a\u91cf\u4e0b\u9650\uff08LOQ\uff09\u9054\u6210\u3092\u76ee\u6a19\u3068\u3057\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u3053\u3046\u3057\u305f\u4f4e\u3044\u691c\u51fa\u9650\u754c\u306e\u9054\u6210\u306f\u3001\u7279\u306b\u611f\u5ea6\u306e\u4f4e\u3044\u65e7\u578b\u88c5\u7f6e\u3067\u306f\u975e\u5e38\u306b\u56f0\u96e3\u3067\u3059\u3002\u5f93\u6765\u306e\u611f\u5ea6\u5411\u4e0a\u7b56\u3092\u3059\u3079\u3066\u8a66\u3057\u3066\u3082\u306a\u304a\u3001\u5341\u5206\u306a\u7d50\u679c\u304c\u5f97\u3089\u308c\u306a\u3044\u5834\u5408\u306b\u306f\u3001\u30a4\u30ca\u30fc\u30c8LC\u30ab\u30e9\u30e0\u306e\u5c0e\u5165\u3092\u305c\u3072\u3054\u691c\u8a0e\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u30a4\u30ca\u30fc\u30c8LC\u30ab\u30e9\u30e0\u306e\u69cb\u9020\u306f\u5f93\u6765\u306e\u30b9\u30c6\u30f3\u30ec\u30b9\u88fdLC\u30ab\u30e9\u30e0\u3068\u540c\u69d8\u3067\u3059\u304c\u3001\u8868\u9762\u306b\u4e0d\u6d3b\u6027\u30b3\u30fc\u30c6\u30a3\u30f3\u30b0\u304c\u65bd\u3055\u308c\u3066\u3044\u308b\u70b9\u3067\u7570\u306a\u308a\u307e\u3059\u3002\u3053\u306e\u30b3\u30fc\u30c6\u30a3\u30f3\u30b0\u306f\u3001\u91d1\u5c5e\u8868\u9762\u306b\u5bfe\u3059\u308b\u5206\u6790\u5bfe\u8c61\u6210\u5206\u306e\u30ad\u30ec\u30fc\u30c8\u5316\u3084\u5438\u7740\u306a\u3069\u306e\u975e\u7279\u7570\u7684\u7d50\u5408\u3092\u9632\u3050\u5f79\u5272\u3092\u6301\u3061\u307e\u3059\u3002\u30ad\u30ec\u30fc\u30c8\u5316\u304c\u8d77\u3053\u308b\u3068\u30d4\u30fc\u30af\u5f62\u72b6\u304c\u60aa\u5316\u3057\u305f\u308a\u3001\u5206\u6790\u5bfe\u8c61\u6210\u5206\u306e\u640d\u5931\u306b\u3088\u308a\u691c\u51fa\u611f\u5ea6\u304c\u4f4e\u4e0b\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u3059\u3079\u3066\u306e\u5316\u5408\u7269\u304c\u975e\u7279\u7570\u7684\u7d50\u5408\uff08\u30ad\u30ec\u30fc\u30c8\u5316\uff09\u3092\u8d77\u3053\u3059\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u7279\u306b\u9178\u6027\u5316\u5408\u7269\u306f\u30ad\u30ec\u30fc\u30c8\u5316\u3057\u3084\u3059\u3044\u50be\u5411\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n

\u30a4\u30ca\u30fc\u30c8\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067\u3001\u3059\u3079\u3066\u306e\u5206\u6790\u5bfe\u8c61\u6210\u5206\u3067\u9055\u3044\u304c\u51fa\u308b\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u691c\u51fa\u611f\u5ea6\u306e\u5411\u4e0a\u306f\u671f\u5f85\u3067\u304d\u307e\u3059\u3002\u4ee5\u4e0b\u306e\u5b9f\u9a13\u3067\u306f\u3001\u5168\u8840\u4e2d\u306e\u30ab\u30f3\u30ca\u30d3\u30ce\u30a4\u30c9\u5206\u6790\u306b\u30a4\u30ca\u30fc\u30c8LC\u30ab\u30e9\u30e0\u3068\u5f93\u6765\u306e\u30b9\u30c6\u30f3\u30ec\u30b9\u88fd\u30ab\u30e9\u30e0\u3092\u7528\u3044\u3066\u3001\u305d\u308c\u305e\u308c\u306e\u6027\u80fd\u3092\u6bd4\u8f03\u3057\u307e\u3057\u305f\u3002\u958b\u767a\u3057\u305f\u30e1\u30bd\u30c3\u30c9\u3067\u306f\u3001Raptor FluoroPhenyl\u56fa\u5b9a\u76f8\u3092\u5229\u7528\u3057\u3001\u03948-THC\u3001\u03949-THC\u304a\u3088\u3073\u305d\u308c\u3089\u306e\u30d2\u30c9\u30ed\u30ad\u30b7\u30fb\u30ab\u30eb\u30dc\u30ad\u30b7\u4ee3\u8b1d\u7269\u3092\u5206\u96e2\u3092\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002\u30a4\u30ca\u30fc\u30c8\u30ab\u30e9\u30e0\u306e\u6709\u52b9\u6027\u3092\u8a55\u4fa1\u3059\u308b\u305f\u3081\u306b\u3001\u5206\u6790\u5bfe\u8c61\u6210\u5206\u3092\u542b\u3080\u30b5\u30f3\u30d7\u30eb\u3092Raptor Inert FluoroPhenyl\u30ab\u30e9\u30e0\u3068Raptor FluoroPhenyl\u30ab\u30e9\u30e0\uff08\u6a19\u6e96\u30ab\u30e9\u30e0\uff09\u306e\u4e21\u65b9\u3067\u76ee\u7684\u6210\u5206\u3092\u542b\u3080\u30b5\u30f3\u30d7\u30eb\u306e\u5206\u6790\u3092\u5b9f\u884c\u3057\u3001\u305d\u306e\u7d50\u679c\u3092\u6bd4\u8f03\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n

Figure 1:<\/strong> Raptor FluoroPhenyl\u30ab\u30e9\u30e0\u3068Raptor Inert FluoroPhenyl\u30ab\u30e9\u30e0\u3067\u306e\u03949-THC\u3001\u03948-THC\u3001\u304a\u3088\u3073\u4ee3\u8b1d\u7269\u306e\u5206\u6790\u7d50\u679c\u6bd4\u8f03\uff08\u5206\u6790\u6761\u4ef6\u306f\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u53ef\uff09<\/div>
\n
\"Comparison<\/div>

LC_CF0829<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th>Conc.
(ng\/mL)<\/th>		Precursor<\/th>Product 1<\/th>Product 2<\/th><\/tr><\/thead>\n
1.<\/td>11-OH-\u03948-THC<\/a><\/td>100<\/td>331.0<\/td>313.0<\/td>201.1<\/td><\/tr>\n
2.<\/td>11-OH-\u03949-THC<\/a><\/td>100<\/td>331.0<\/td>313.0<\/td>201.1<\/td><\/tr>\n
3.<\/td>\u03948-THC-COOH<\/a><\/td>500<\/td>345.1<\/td>327.0<\/td>299.2<\/td><\/tr>\n
4.<\/td>\u03949-THC-COOH<\/a><\/td>500<\/td>345.1<\/td>327.0<\/td>299.2<\/td><\/tr>\n
5.<\/td>\u03948-THC<\/a><\/td>100<\/td>315.0<\/td>193.0<\/td>123.2<\/td><\/tr>\n
6.<\/td>\u03949-THC<\/a><\/td>100<\/td>315.0<\/td>193.0<\/td>123.2<\/td><\/tr>\n<\/tbody><\/table><\/div>

Conditions<\/h4>
Column<\/th>See notes.<\/td><\/tr>
Dimensions:<\/th>100 mm x 3 mm ID<\/td><\/tr>
Particle Size:<\/th>2.7 \u00b5m<\/td><\/tr>
Pore Size:<\/th>90 \u00c5<\/td><\/tr>
<\/td>
Temp.:<\/th>40 \u00b0C<\/td><\/tr>
Standard\/Sample<\/th><\/td><\/tr>
<\/td>\u03948-Tetrahydrocannabinol (\u03948-THC) (cat.# 34090<\/a>)<\/td><\/tr>
<\/td>\u03949-Tetrahydrocannabinol (\u03949-THC) (cat.# 34067<\/a>)<\/td><\/tr>
<\/td>(\u00b1)11-nor-9-carboxy-\u0394-9-THC (\u03949-THC-COOH) (cat.# 34068<\/a>)<\/td><\/tr>
<\/td>Other compounds obtained separately. <\/td><\/tr>\n
Diluent:<\/th>40:60 Water:methanol, both with 0.1% formic acid (v\/v)<\/td><\/tr><\/td><\/tr>
Inj. Vol.:<\/th>5 \u00b5L <\/td><\/tr>
Mobile Phase<\/th><\/td><\/tr>
A:<\/th>Water, 0.1% formic acid <\/td><\/tr>
B:<\/th>Methanol, 0.1% formic acid <\/td><\/tr>
<\/td>
Time (min)<\/th>Flow (mL\/min)<\/th>%A<\/th>%B<\/th><\/tr><\/thead>
0.00<\/td>0.8<\/td>36<\/td>64<\/td><\/tr>
6.50<\/td>0.8<\/td>36<\/td>64<\/td><\/tr>
6.60<\/td>0.8<\/td>32<\/td>68<\/td><\/tr>
13.00<\/td>0.8<\/td>32<\/td>68<\/td><\/tr>
13.10<\/td>0.8<\/td>0<\/td>100<\/td><\/tr>
14.00<\/td>0.8<\/td>0<\/td>100<\/td><\/tr>
14.10<\/td>0.8<\/td>36<\/td>64<\/td><\/tr>
16.00<\/td>0.8<\/td>36<\/td>64<\/td><\/tr><\/tbody><\/table><\/td><\/tr>
Max Pressure:<\/th>390 bar<\/td><\/tr><\/table>
Detector<\/th>Shimadzu 8045 MS\/MS<\/td><\/tr>
Ion Mode:<\/th>ESI+ <\/td><\/tr>
Instrument<\/th>Shimadzu Nexera X2<\/td><\/tr>
Sample Preparation<\/th>Five hundred microliters of whole blood was transferred to a 12 mL glass test tube. Fifty microliters of internal standard and 50 \u00b5L of control material were transferred to the test tube and vortexed. Five hundred microliters of HPLC grade water was added to each sample and vortexed. One hundred microliters of 10% acetic acid was added to each sample and vortexed. Two and a half milliliters of 80:20 hexanes:ethyl acetate was added to each sample and vortexed until visibly combined. Samples were centrifuged at 2800 rpm for 15 minutes. The top layer was transferred to a new glass test tube and dried down under nitrogen. Samples were reconstituted with 100 \u00b5L of 40:60 methanol:water, both containing 0.1% formic acid, and vortexed. Samples were transferred to 2 mL screw-thread vials (cat.# 21143<\/a>) with glass inserts (cat.# 21776<\/a>) and capped with short-cap, screw-vial closures (cat.# 24498<\/a>).<\/td><\/tr>
Notes<\/th>The column was stored in 100% acetonitrile when not in use.

Columns are:

Raptor Inert FluoroPhenyl (cat.# 9319A1E-T<\/a>)
Raptor FluoroPhenyl (cat.# 9319A1E<\/a>)<\/td><\/tr><\/table><\/div><\/div><\/div>
<\/path><\/g><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n
<\/div>\n\n\n\n

\u3053\u306e\u5b9f\u9a13\u7d50\u679c\u304b\u3089\u3001\u30a4\u30ca\u30fc\u30c8\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067\u30016\u7a2e\u985e\u3059\u3079\u3066\u306e\u5206\u6790\u5bfe\u8c61\u6210\u5206\u306b\u304a\u3044\u3066\u30d4\u30fc\u30af\u9ad8\u3055\u304a\u3088\u3073\u9762\u7a4d\u306e\u5897\u52a0\u304c\u3042\u3063\u305f\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\u3002\u89aa\u5316\u5408\u7269\u306b\u3064\u3044\u3066\u306f\u30d4\u30fc\u30af\u9ad8\u3055\u30fb\u9762\u7a4d\u306e\u5897\u52a0\u306f2\uff5e7%\u3068\u308f\u305a\u304b\u3067\u3057\u305f\u304c\u3001\u30d2\u30c9\u30ed\u30ad\u30b7\u4ee3\u8b1d\u7269\u3067\u306f17\uff5e27%\u3001\u30ab\u30eb\u30dc\u30ad\u30b7\u4ee3\u8b1d\u7269\u3067\u306f\u3055\u3089\u306b\u5927\u304d\u304f31\uff5e39%\u3068\u3001\u7279\u306b\u4ee3\u8b1d\u7269\u3067\u9855\u8457\u306a\u5411\u4e0a\u304c\u898b\u3089\u308c\u307e\u3057\u305f\u3002\u3068\u308a\u308f\u3051\u30ab\u30eb\u30dc\u30ad\u30b7\u5316\u5408\u7269\u3067\u691c\u51fa\u611f\u5ea6\u304c\u5927\u304d\u304f\u5411\u4e0a\u3057\u305f\u306e\u306f\u3001\u30ab\u30eb\u30dc\u30f3\u9178\u5b98\u80fd\u57fa\u304c\u91d1\u5c5e\u8868\u9762\u3068\u975e\u7279\u7570\u7684\u306b\u7d50\u5408\u3057\u3084\u3059\u3044\u3053\u3068\u304c\u4e3b\u306a\u8981\u56e0\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u4e00\u65b9\u3001\u30d2\u30c9\u30ed\u30ad\u30b7\u5316\u5408\u7269\u3084\u89aa\u5316\u5408\u7269\u306f\u3088\u308a\u4e2d\u6027\u3067\u3042\u308b\u305f\u3081\u3001\u30a4\u30ca\u30fc\u30c8\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3057\u305f\u3053\u3068\u306b\u3088\u308b\u5f71\u97ff\u306f\u6bd4\u8f03\u7684\u9650\u5b9a\u7684\u3067\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u7d50\u679c\u304b\u3089\u3001\u30a4\u30ca\u30fc\u30c8\u30ab\u30e9\u30e0\u306e\u5c0e\u5165\u306f\u30ab\u30f3\u30ca\u30d3\u30ce\u30a4\u30c9\u306e\u691c\u51fa\u611f\u5ea6\u5411\u4e0a\u306b\u6709\u52b9\u3067\u3042\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u307e\u3059\u3002<\/p>\n\n\n\n

\u5168\u8840\u4e2d\u306e\u30ab\u30f3\u30ca\u30d3\u30ce\u30a4\u30c9\u5206\u6790\u306b\u304a\u3044\u3066\u4f4e\u3044\u691c\u51fa\u9650\u754c\u306e\u9054\u6210\u306b\u8ab2\u984c\u304c\u3042\u308b\u5834\u5408\u3001\u30a4\u30ca\u30fc\u30c8LC\u30ab\u30e9\u30e0\u306e\u5c0e\u5165\u306f\u6709\u52b9\u306a\u9078\u629e\u80a2\u3068\u306a\u308a\u5f97\u307e\u3059\u3002\u751f\u4f53\u6db2\u4e2d\u306eTHC\u7570\u6027\u4f53\u306e\u5206\u6790\u306b\u95a2\u5fc3\u304c\u3042\u308b\u65b9\u306f\u3001\u3055\u3089\u306a\u308b\u60c5\u5831\u3092\u300c\u5168\u8840\u30fb\u5c3f\u4e2dTHC\u7570\u6027\u4f53\u304a\u3088\u3073\u4ee3\u8b1d\u7269\u306e\u9ad8\u611f\u5ea6LC-MS\/MS\u30e1\u30bd\u30c3\u30c9<\/a>\u300d\u304b\u3089\u3001\u305c\u3072\u3054\u78ba\u8a8d\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

<\/div>\n\n\n
\n

Products Mentioned<\/h3>\n
\n
\n
\n
\n Catalog No. 9319A1E-T <\/a> <\/div>\n
\n
Raptor Inert FluoroPhenyl, 2.7 \u00b5m, 100 x 3.0 mm HPLC\u30ab\u30e9\u30e0<\/div>\n <\/div>\n
\n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 34090 <\/a> <\/div>\n
\n
delta 8-Tetrahydrocannabinol (Delta 8-THC) Standard, 1000 \u00b5g\/mL in P&T Methanol, 1 mL\/ampul<\/div>\n <\/div>\n
\n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 34067 <\/a> <\/div>\n
\n
delta 9-Tetrahydrocannabinol (Delta 9-THC) Standard, 1000 \u00b5g\/mL, P&T Methanol, 1 mL\/ampul<\/div>\n <\/div>\n
\n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 34068 <\/a> <\/div>\n
\n
(\u00b1)11-nor-9-carboxy-Delta-9-THC Standard, 100 \u00b5g\/mL, methanol, 1 mL\/ampul<\/div>\n <\/div>\n
\n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 21143 <\/a> <\/div>\n
\n
Short-Cap Vial with Grad Marking Spot, 9-425 Screw-Thread, 2.0 mL, 9 mm, 12 x 32 (vial only), Amber, 1000-pk.<\/div>\n <\/div>\n
\n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 21776 <\/a> <\/div>\n
\n
Vial Inserts, Glass, Big Mouth w\/Bottom Spring, 250 \u00b5L, 100-pk.<\/div>\n <\/div>\n
\n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n
\n
\n Catalog No. 24498 <\/a> <\/div>\n
\n
Short Screw Cap, Polypropylene, Screw-Thread, PTFE\/Silicone\/PTFE Septa, Blue, Preassembled, 2.0 mL, 9 mm, 1000-pk.<\/div>\n <\/div>\n
\n \u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n \n\n\n
<\/div>\n","protected":false},"excerpt":{"rendered":"

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