{"id":48565,"date":"2014-11-06T13:30:00","date_gmt":"2014-11-06T13:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/japanese-how-to-decrease-run-time-for-reversed-phase-lc\/"},"modified":"2025-11-25T17:59:58","modified_gmt":"2025-11-25T17:59:58","slug":"japanese-how-to-decrease-run-time-for-reversed-phase-lc","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/blogs-ja\/gnbl4708-ja\/japanese-how-to-decrease-run-time-for-reversed-phase-lc","title":{"rendered":"\u9006\u76f8LC\u3067\u5206\u6790\u6642\u9593\u3092\u77ed\u304f\u3059\u308b\u65b9\u6cd5"},"content":{"rendered":"\n

The original English blog was posted on November 7th, 2014 by Nancy Schwartz<\/p>\n\n\n\n

\u9006\u76f8LC\u306e\u9ad8\u901f\u5316\u306b\u306f\u3044\u304f\u3064\u304b\u306e\u65b9\u6cd5\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u304a\u304a\u3088\u305d4\u3064\u306b\u307e\u3068\u3081\u3089\u308c\u307e\u3059\u3002<\/p>\n\n\n\n

1.  \u77ed\u3044\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3059\u308b<\/p>\n\n\n\n

\u5206\u6790\u5bfe\u8c61\u7269\u304c\u5341\u5206\u306b\u4fdd\u6301\u3055\u308c\u3066\u3044\u308b\u3068\u304d\uff0c\u5206\u6790\u5bfe\u8c61\u7269\u304c1\u7a2e\u985e\u3060\u3051\u3082\u3057\u304f\u306f\u5c11\u6570\u3067\uff0c\u304b\u3064\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30e0\u4e0a\u3067\u8fd1\u63a5\u3059\u308b\u30d4\u30fc\u30af\u304c\u306a\u3044\u3068\u304d\u306f\u3001\u3053\u306e\u65b9\u6cd5\u304c\u9069\u3057\u3066\u3044\u308b\u3067\u3057\u3087\u3046\u3002\u305f\u3060\u3057\u3001\u65b0\u3057\u3044\u30ab\u30e9\u30e0\u3092\u8cfc\u5165\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u3001\u9577\u3055\uff08L\uff09\u3092\u77ed\u304f\u3059\u308b\u3068\u3001\u30ab\u30e9\u30e0\u306e\u7406\u8ad6\u6bb5\u6570\uff08N\uff09\u3082\u5c11\u306a\u304f\u306a\u308a\u307e\u3059\u3002\u826f\u3044\u30cb\u30e5\u30fc\u30b9\u306f\u3001\u6700\u7d42\u7d50\u679c\u304c\u4e88\u60f3\u3088\u308a\u3082\u826f\u3044\u304b\u3082\u3057\u308c\u306a\u3044\u3068\u3044\u3046\u3053\u3068\u3067\u3059\u3002\u30ab\u30e9\u30e0\u306e\u9577\u3055\u30922\u5206\u306e1\u306b\u77ed\u7e2e\u3057\u3066\u3082\u3001\u5206\u96e2\u5ea6(R)\u306fN\u306e\u5e73\u65b9\u6839\u306b\u6bd4\u4f8b\u3059\u308b\u305f\u30811.4\u5206\u306e\uff11\u306b\u306a\u308a\uff0cR\u306f2\u5206\u306e1\u306b\u306f\u306a\u308a\u307e\u305b\u3093\u3002<\/p>\n\n\n\n

\"R<\/figure>\n\n\n\n
\"\"<\/figure>\n\n\n\n

2. \u65e2\u5b58\u30ab\u30e9\u30e0\u3067\u6761\u4ef6\u3092\u6700\u9069\u5316\u3059\u308b<\/p>\n\n\n\n

\u4ee5\u4e0b\u3088\u308a\u5fae\u8abf\u6574\u3067\u304d\u305d\u3046\u306a\u70b9\u304c\u306a\u3044\u304b\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044<\/p>\n\n\n\n

\u6d41\u91cf<\/strong> \u2013 \u307e\u305a\uff0c\u6700\u9069\u6d41\u91cf\u672a\u6e80\u3067\u5206\u6790\u3092\u884c\u3063\u3066\u3044\u308b\u306e\u3067\u3042\u308c\u3070\uff0c\u30b7\u30b9\u30c6\u30e0\u306e\u8a31\u5bb9\u7bc4\u56f2\u5185\u3067\u3082\u3046\u5c11\u3057\u6d41\u91cf\u3092\u4e0a\u3052\u308b\u3079\u304d\u3067\u3057\u3087\u3046\u3002\u6b21\u306b\u5b8c\u5168\u306b\u5bfe\u79f0\u306a\u30d4\u30fc\u30af\u5f62\u72b6\u3084\u4ed6\u306e\u30d4\u30fc\u30af\u3068\u306e\u5206\u96e2\u3092\u5fc5\u8981\u3068\u3057\u306a\u3044\u306e\u3067\u3042\u308c\u3070\uff0c\u6700\u9069\u3068\u3055\u308c\u308b\u6d41\u91cf\u3092\u5c11\u3005\u4e0a\u56de\u308b\u8a2d\u5b9a\u3082\u53ef\u80fd\u3067\u3059\u3002<\/p>\n\n\n\n

\u79fb\u52d5\u76f8<\/strong> \u2013 \u307e\u3060\u78ba\u8a8d\u3057\u3066\u3044\u306a\u3044\u306e\u306a\u3089\uff0c\u79fb\u52d5\u76f8\u306e\u6709\u6a5f\u6eb6\u5a92\u6fc3\u5ea6\u3092\u5897\u3084\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002\u4ed6\u306e\u6709\u6a5f\u6eb6\u5a92\u3092\u8a66\u3057\u3066\u307f\u308b\u3053\u3068\u3082\u3067\u304d\u307e\u3059\u3002Biphenyl\u306e\u3088\u3046\u306a\u56fa\u5b9a\u76f8\u3067\u306f\u30e1\u30bf\u30ce\u30fc\u30eb\u306e\u307b\u3046\u304c\u826f\u3044\u9078\u629e\u6027\u3092\u793a\u3059\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u4e00\u65b9\uff0c\u30a2\u30bb\u30c8\u30cb\u30c8\u30ea\u30eb\u306f\u4e00\u822c\u7684\u306b\u6975\u6027\u304c\u4f4e\u3044\u305f\u3081\u4fdd\u6301\u304c\u77ed\u304f\u306a\u308b\u3068\u3044\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u6eb6\u5a92\u306f\u5206\u6790\u6761\u4ef6\u6bce\u306b\u9078\u629e\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u304c\uff0c\u6642\u306b\u306f\u305d\u306e\u52b9\u679c\u306b\u9a5a\u304b\u3055\u308c\u308b\u3053\u3068\u304c\u3042\u308b\u3067\u3057\u3087\u3046\u3002\uff08\u306a\u304a\uff0c\u3053\u308c\u306f\u9006\u76f8LC\u306e\u5834\u5408\u306e\u307f\u3067\u3059\u306e\u3067\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002\uff09<\/p>\n\n\n\n

\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8<\/strong> \u2013 \u521d\u671f\u306e\u6709\u6a5f\u6eb6\u5a92\u6fc3\u5ea6\u3092\u9ad8\u3081\u306b\u8a2d\u5b9a\u3057\u305f\u308a\uff0c\u3088\u308a\u901f\u304f\u6709\u6a5f\u6eb6\u5a92\u6fc3\u5ea6\u304c\u4e0a\u304c\u308b\u3088\u3046\u306a\u52fe\u914d\u3092\u8a66\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

\u6e29\u5ea6<\/strong> \u2013 \u6e29\u5ea6\u3092\u4e0a\u3052\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002\u3053\u308c\u306f\u901a\u5e38\u7269\u4e8b\u3092\u30b9\u30d4\u30fc\u30c9\u30a2\u30c3\u30d7\u3057\uff0c\u5206\u96e2\u3092\u5411\u4e0a\u3055\u305b\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002\u306a\u304a\uff0c\u30ab\u30e9\u30e0\u3067\u63a8\u5968\u3055\u308c\u3066\u3044\u308b\u6e29\u5ea6\u306f\u8d85\u3048\u306a\u3044\u3088\u3046\u306b\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002Restek \u88fd HPLC\u30ab\u30e9\u30e0\u306e\u4e00\u822c\u7684\u306a\u4e0a\u9650\u6e29\u5ea6\u306f80\u2103\u3067\u3059\u3002<\/p>\n\n\n\n

3. \u7c92\u5b50\u5f84\u306e\u5c0f\u3055\u3044\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3059\u308b<\/p>\n\n\n\n

\u30ab\u30e9\u30e0\u52b9\u7387\u304c\u5411\u4e0a\u3057\u3001\u9ad8\u6d41\u91cf\u3067\u3082\u52b9\u7387\u304c\u7dad\u6301\u3055\u308c\u307e\u3059\u3002\u3053\u308c\u306f\u3001\u4e0a\u306e2\u3064\u306e\u5f0f\u3067\u793a\u3055\u308c\u308b\u3088\u3046\u306b\u3001\u5206\u96e2\u306e\u52b9\u7387\u306f\u7c92\u5b50\u5f84\u306b\u53cd\u6bd4\u4f8b\u3059\u308b\u305f\u3081\u3067\u3059\u3002\u4e0b\u306e\u30b0\u30e9\u30d5\u306f\u3001\u7406\u8ad6\u6bb5\u9ad8\u3055\u306b\u76f8\u5f53\u3059\u308bH\u306b\u5bfe\u3059\u308b\u7dda\u901f\u5ea6\u306e\u5f71\u97ff\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002H\u306e\u5024\u304c\u5c0f\u3055\u3044\u307b\u3069\u5206\u96e2\u52b9\u7387\u304c\u9ad8\u304f\u3001\u5358\u4f4d\u9577\u3055\uff08L\uff09\u3042\u305f\u308a\u306e\u6bb5\u6570\uff08N\uff09\u304c\u591a\u3044\u3053\u3068\u3092\u610f\u5473\u3057\u307e\u3059\u3002<\/p>\n\n\n

\n
\"H<\/figure>\n<\/div>\n\n\n

\u5358\u4f4d\u9577\u3055\u3042\u305f\u308a\u306e\u6bb5\u6570\u304c\u5897\u3048\u308b\u306e\u3067\u3001\u540c\u3058\u9577\u3055\u3092\u7dad\u6301\u3057\u3066\u5206\u96e2\u3092\u6539\u5584\u3059\u308b\u3053\u3068\u3082\u3001\u77ed\u3044\u5206\u6790\u6642\u9593\u3067\u540c\u3058\u5206\u96e2\u304c\u5f97\u3089\u308c\u308b\u3088\u3046\u306b\u9069\u5ea6\u306b\u30ab\u30e9\u30e0\u3092\u77ed\u304f\u3059\u308b\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002\u7c92\u5b50\u5f84\u3068\u30ab\u30e9\u30e0\u9577\u306e\u95a2\u4fc2\u306f\u6b21\u306e\u5f0f\u3067\u8868\u3055\u308c\u307e\u3059\u3002<\/p>\n\n\n

\n
\"L<\/figure>\n<\/div>\n\n\n

\u5c0f\u3055\u3044\u7c92\u5b50\u5f84\u306b\u5bfe\u3059\u308b\u5225\u306e\u9078\u629e\u80a2\u306b\u306f\u6d41\u91cf\u3092\u4e0a\u3052\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002\u3068\u3044\u3046\u306e\u306f\u30ab\u30e9\u30e0\u30b5\u30a4\u30ba\u304c\u540c\u3058\u3067\u3042\u308b\u306a\u3089\uff0c\u540c\u3058\u56fa\u5b9a\u76f8\u3067\u7c92\u5b50\u5f84\u306e\u5927\u304d\u3044\u30ab\u30e9\u30e0\u3068\u6bd4\u8f03\u3057\u3066\uff0c\u9ad8\u6d41\u91cf\u3067\u3082\u52b9\u7387\u304c\u843d\u3061\u306a\u3044\u304b\u3089\u3067\u3059\u3002\u53c2\u8003\u3068\u3057\u3066\uff0c\u4e0a\u306eH\u5bfe\u7dda\u901f\u5ea6\u00b5\u306e\u30b0\u30e9\u30d5\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002\u306a\u304a\uff0c\u5185\u5f84\u3092\u5c0f\u3055\u304f\u3059\u308b\u3068\u304d\u540c\u3058\u7dda\u901f\u5ea6\u3092\u5f97\u308b\u305f\u3081\u306b\u306f\uff0c\u6d41\u91cf\u3082\u4e0b\u3052\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u305f\u3081\uff0c\u3053\u306e\u70b9\u3082\u8003\u616e\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u4e00\u822c\u7684\u306b\u306f\u5185\u5f84\u3092\u5c0f\u3055\u304f\u3059\u308b\u3053\u3068\u306f\u5206\u6790\u6642\u9593\u3092\u77ed\u7e2e\u3059\u308b\u305f\u3081\u3067\u306f\u306a\u304f\uff0c\u6eb6\u5a92\u3092\u7bc0\u7d04\u3059\u308b\u305f\u3081\u306b\u6709\u7528\u3067\u3059\u3002<\/p>\n\n\n\n

\u7c92\u5b50\u5f84\u3092\u5c0f\u3055\u304f\u3059\u308b\u3068\u80cc\u5727\u3082\u4e0a\u304c\u308b\u306e\u3067\uff0c\u4f7f\u7528\u3059\u308b\u7c92\u5b50\u5f84\u3092\u6c7a\u3081\u308b\u969b\u306b\u306f\u88c5\u7f6e\u306e\u8010\u5727\u3082\u8003\u616e\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u5f53\u793e\u306eForce\u306a\u3069\u30b5\u30d62 \u00b5m\u7c92\u5b50\u306e\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3059\u308b\u306b\u306f\u3001UHPLC\u30b7\u30b9\u30c6\u30e0\uff08\u6700\u59271000 bar\u4ee5\u4e0a\u306e\u5727\u529b\u306b\u5bfe\u5fdc\uff09\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n\n\n\n

4. \u8868\u9762\u591a\u5b54\u8cea(superficially porous particle : SPP)\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3059\u308b<\/p>\n\n\n\n

Restek\u306e\u88fd\u54c1\u7fa4\u3067\u8a00\u3046\u3068\uff0cRaptor\u30ab\u30e9\u30e0\u304c\u8a72\u5f53\u3057\u307e\u3059\u3002\u30b3\u30a2\u30b7\u30a7\u30eb\u30ab\u30e9\u30e0\u306e\u5229\u70b9\u306b\u3064\u3044\u3066\u306e\u7c21\u5358\u306a\u8aac\u660e\u304c\u300cSPP\u3063\u3066\u4f55\uff1f\u3069\u3093\u306a\u3068\u304d\u306bRaptor\u30ab\u30e9\u30e0\u3092\u4f7f\u3046\u3079\u304d\uff1f\u300d\u3068\u3044\u3046\u30d6\u30ed\u30b0\u8a18\u4e8b\u306b\u3042\u308a\u307e\u3059\u3002SPP\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3059\u308b\u3068\u30d4\u30fc\u30af\u304c\u5927\u5e45\u306b\u30b7\u30e3\u30fc\u30d7\u306b\u306a\u308b\u305f\u3081\uff0c\u5206\u6790\u306e\u5168\u4f53\u7684\u306a\u611f\u5ea6\u3082\u5411\u4e0a\u3057\u307e\u3059\u3002\u3057\u3070\u3057\u3070UHPLC\u3067\u306f\u306a\u3044LC\u3092\u4f7f\u7528\u3057\u3066UHPLC\u30ec\u30d9\u30eb\u306e\u30c7\u30fc\u30bf\u304c\u5f97\u3089\u308c\u3066\u3044\u307e\u3059\u30022.7 \u00b5m \u3068 5 \u00b5m\u3069\u3061\u3089\u306e\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3059\u308b\u304b\u30d2\u30f3\u30c8\u304c\u307b\u3057\u3044\u5834\u5408\u306f\uff0c\u300c2.7um\u30685um\u3001\u3069\u3061\u3089\u306eRaptor\u30ab\u30e9\u30e0\u3092\u4f7f\u3048\u3070\u3088\u3044\u306e\u3067\u3057\u3087\u3046\u304b\uff1f\u300d\u3068\u3044\u3046\u30d6\u30ed\u30b0\u8a18\u4e8b\u3092\u3054\u53c2\u8003\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n

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