{"id":51641,"date":"2022-01-05T13:30:00","date_gmt":"2022-01-05T13:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/chiral-separations-6-essential-oils-on-rt-dexsm\/"},"modified":"2026-01-28T15:50:54","modified_gmt":"2026-01-28T15:50:54","slug":"chiral-separations-6-essential-oils-on-rt-dexsm","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/blogs\/gnbl4623\/chiral-separations-6-essential-oils-on-rt-dexsm","title":{"rendered":"Chiral Separations 6: Essential oils on Rt-\u03b2DEXsm"},"content":{"rendered":"\n

Chiral capillary GC has proven to be a convenient method for characterizing essential oils and differentiating natural flavors from those of synthetic origin. Chiral compounds from natural origins usually exist as one predominant optical isomer. Also, the inspection of enantiomeric ratios can characterize regional differences between oils. Sometimes a result of processing, the presence of racemic pairs (one-to-one ratios of each enantiomer) most often indicates adulteration or unnatural origin.<\/p>\n\n\n\n

Since most chiral compounds naturally exist as one predominant isomer, resolution is more challenging, especially for components in higher concentrations. For primary constituents in essential oils, it is preferable to select a chiral column that provides a resolution factor value greater than two to overcome possible loss of resolution.<\/p>\n\n\n\n

Lemon Oil<\/h2>\n\n\n\n

The Rt-\u03b2DEXsm is the optimum column for obtaining chiral profiles of Lemon and other citrus oils since it provides enantiomeric separation for the main terpene constituents like \u03b1- and \u03b2-pinenes, and limonene.<\/p>\n\n\n\n

\"a
GC_FF1353<\/figcaption><\/figure>\n\n\n\n
 <\/th>Peaks<\/th>tR<\/sub> 1<\/th>tR<\/sub> 2<\/th><\/tr><\/thead>
1.<\/td>\u03b1-Pinene (\u00b1)<\/a><\/td>10.544<\/td>10.980<\/td><\/tr>
2.<\/td>\u03b2-Pinene (\u00b1)<\/a><\/td>12.681<\/td>13.136<\/td><\/tr>
3.<\/td>Limonene (L, D)<\/a><\/td>14.826<\/td>15.342<\/td><\/tr>
4.<\/td>\u03b3-Terpinene<\/a><\/td>17.764<\/td>–<\/td><\/tr>
5.<\/td>\u03b1-Terpineol (\u00b1)<\/a><\/td>33.546<\/td>34.248<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Download PDF<\/a><\/p>\n\n\n\n

Figure 1: Lemon oil on Rt-\u03b2DEXsm, GC_FF1353<\/p>\n\n\n\n

Rosemary Oil<\/h2>\n\n\n\n

Chiral constituents in this oil include \u03b1-pinene, \u03b2-pinene, camphene, limonene, linalool, camphor, terpinen- 4-ol, \u03b1 terpineol, borneol, and isoborneol. These compounds are resolved optimally on the Rt- \u03b2DEXsm column.<\/p>\n\n\n\n

\"a
GC_FF1355<\/figcaption><\/figure>\n\n\n\n
 <\/th>Peaks<\/th>tR<\/sub>1<\/th>tR<\/sub>2<\/th><\/tr><\/thead>
1.<\/td>\u03b1-Pinene (\u00b1)<\/a><\/td>10.500<\/td>10.941<\/td><\/tr>
2.<\/td>Camphene (\u00b1)<\/a><\/td>11.566<\/td>11.952<\/td><\/tr>
3.<\/td>\u03b2-Pinene (\u00b1)<\/a><\/td>12.690<\/td>13.200<\/td><\/tr>
4.<\/td>Limonene (\u00b1)<\/a><\/td>14.850<\/td>15.585<\/td><\/tr>
5.<\/td>Eucalyptol (1,8-cineole)<\/a><\/td>15.837<\/td>–<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
 <\/th>Peaks<\/th>tR<\/sub>1<\/th>tR<\/sub>2<\/th><\/tr><\/thead>
6.<\/td>Linalool (\u00b1)<\/a><\/td>26.011<\/td>26.463<\/td><\/tr>
7.<\/td>Camphor (\u00b1)<\/a><\/td>27.492<\/td>27.704<\/td><\/tr>
8.<\/td>Terpinen-4-ol (\u00b1)<\/a><\/td>30.647<\/td>30.935<\/td><\/tr>
9.<\/td>Isoborneol (\u00b1)<\/a><\/td>32.254<\/td>32.786<\/td><\/tr>
10.<\/td>Borneol (\u00b1)<\/a><\/td>33.000<\/td>33.756<\/td><\/tr>
11.<\/td>\u03b1-Terpineol (\u00b1)<\/a><\/td>33.539<\/td>34.237<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Download PDF<\/a><\/p>\n\n\n\n

Figure 2: Rosemary oil on Rt-\u03b2DEXsm, GC_FF1355<\/p>\n\n\n\n

Peppermint Oil<\/h2>\n\n\n\n

The Rt-\u03b2DEXsm column is optimum for the separation of (+\/-) \u03b1- and \u03b2-pinene, and limonene. Since menthone and menthol enantiomers are major constituents of peppermint oil, reducing the sample size to prevent overloading of these components and provide better enantiomeric resolution may be necessary. An alternative solution is to use an Rt-\u03b2DEXm or Rt-\u03b3DEXsa as a secondary column since they provide better resolution of menthol.<\/p>\n\n\n\n

\"\"
GC_FF1356<\/figcaption><\/figure>\n\n\n\n
 <\/th>Peaks<\/th>tR<\/sub> 1<\/th>tR<\/sub> 2<\/th><\/tr><\/thead>
1.<\/td>\u03b1-Pinene (\u00b1)<\/a><\/td>16.214<\/td>16.654<\/td><\/tr>
2.<\/td>\u03b2-Pinene (\u00b1)<\/a><\/td>18.836<\/td>19.404<\/td><\/tr>
3.<\/td>Limonene (L,D)<\/a><\/td>21.396<\/td>22.216<\/td><\/tr>
4.<\/td>Menthone<\/a><\/td>32.738<\/td>–<\/td><\/tr>
5.<\/td>Menthol<\/a><\/td>39.340<\/td>–<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Download PDF<\/a><\/p>\n\n\n\n

\"blog-chiral-blog-6-essential-oils-on-RT-BDEXsm-03.png\"<\/figure>\n\n\n\n

Figure 3: Peppermint oil on Rt-\u03b2DEXsm, GC_FF1356<\/p>\n\n\n\n

In the next blog, we\u2019ll look into dual column analysis of lavender oil.<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Chiral capillary GC has proven to be a convenient method for characterizing essential oils and differentiating natural flavors from those of synthetic origin. Chiral compounds from natural origins usually exist as one predominant optical isomer. Also, the inspection of enantiomeric ratios can characterize regional differences between oils. Sometimes a result of processing, the presence of racemic pairs (one-to-one ratios of each enantiomer) most often indicates adulteration or unnatural origin.<\/p>\n","protected":false},"author":50,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[9],"tags":[],"industries-application":[2149,2167],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[2294],"ppma_author":[591],"class_list":["post-51641","post","type-post","status-publish","format-standard","hentry","category-blogs","industries-application-cannabis","industries-application-food-beverage","resource-technique-gas-chromatography-gc"],"acf":[],"taxonomy_info":{"category":[{"value":9,"label":"Blogs"}],"industries-application":[{"value":2149,"label":"Cannabis"},{"value":2167,"label":"Food & Beverage"}],"resource-technique":[{"value":2294,"label":"Gas Chromatography (GC)"}]},"featured_image_src_large":false,"author_info":{"display_name":"Jana Hepner, PhD","author_link":"https:\/\/discover.restek.com\/ja\/author\/jana-hepner-phd\/"},"comment_info":0,"category_info":[{"term_id":9,"name":"Blogs","slug":"blogs","term_group":0,"term_taxonomy_id":9,"taxonomy":"category","description":"","parent":0,"count":438,"filter":"raw","cat_ID":9,"category_count":438,"category_description":"","cat_name":"Blogs","category_nicename":"blogs","category_parent":0}],"tag_info":false,"authors":[{"term_id":591,"user_id":50,"is_guest":0,"slug":"jana-hepner-phd","display_name":"Jana Hepner, PhD","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-hepner-jana.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-hepner-jana.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/51641","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/users\/50"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/comments?post=51641"}],"version-history":[{"count":3,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/51641\/revisions"}],"predecessor-version":[{"id":85258,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/posts\/51641\/revisions\/85258"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/media?parent=51641"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/categories?post=51641"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/tags?post=51641"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/industries-application?post=51641"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/post-badge?post=51641"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-type?post=51641"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/product-library?post=51641"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/resource-technique?post=51641"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/ja\/wp-json\/wp\/v2\/ppma_author?post=51641"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}