{"id":51843,"date":"2025-03-24T00:00:00","date_gmt":"2025-03-24T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/using-the-ezlc-modeler-for-cannabinoid-separations-part-3\/"},"modified":"2026-01-28T16:02:57","modified_gmt":"2026-01-28T16:02:57","slug":"using-the-ezlc-modeler-for-cannabinoid-separations-part-3","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/blogs\/gnbl4850\/using-the-ezlc-modeler-for-cannabinoid-separations-part-3","title":{"rendered":"Using the <em>EZ<\/em>LC Modeler for Cannabinoid Separations\u2013Part 3: What You\u2019re Not Monitoring for Still Matters"},"content":{"rendered":"\n<p><style>     ul li { margin-top: 5px; }     ol li { margin-top: 5px; }  <\/style>If you haven\u2019t read the first two installments of this four-part blog series, they can be found here: <a href=\"https:\/\/discover.restek.com\/gnbl4848\/using-the-ezlc-modeler-for-cannabinoid-separations-part-1\/\">Optimization of an Existing Method<\/a>&nbsp;and <a href=\"https:\/\/discover.restek.com\/gnbl4849\/using-the-ezlc-modeler-for-cannabinoid-separations-part-2\/\">Using <em>EZ<\/em>LC Software to Monitor Effects of Ammonium Formate Concentrations on Cannabinoid Separations<\/a>.<\/p>\n\n\n\n<p>While target analytes in the method are the main focus, it is also important to know what could be in your sample that you are not monitoring for. A prime example of this is when a regulatory body rolled out a method in 2019. The disseminated regulations, which later received many revisions, were for cannabinoid testing of dried flower, including non-infused pre-rolls. The criterion for this testing is as follows:<\/p>\n\n\n\n<p>1. Compounds: CBDA, CBG, CBD, THCV, CBN, \u03949-THC, \u03948-THC, CBC, THCA<\/p>\n\n\n\n<p>2. HPLC Column: Restek Raptor ARC-18 2.1 x 150 mm, 2.7 \u00b5m (cat.#&nbsp;9314A62) or an equivalent column that can separate the cannabinoids of interest to achieve a minimum resolution of 1.3<\/p>\n\n\n\n<p>3. Mobile Phases:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Mobile phase A: Water with 0.05 volume\/volume% formic acid<\/li>\n\n\n\n<li>Mobile phase B: Acetonitrile with 0.05 volume\/volume% formic acid<\/li>\n<\/ul>\n\n\n\n<p>4. Flow Rate: 0.4 mL\/min<\/p>\n\n\n\n<p>5. Run time: total 12.00 min (7.00 min + 2.00 min washing period + 3.00 min column re-equilibration)<\/p>\n\n\n\n<p>6. Column Temperature: 35 \u2070C<\/p>\n\n\n\n<p>7. Gradient:<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td><strong>Time (min)<\/strong><\/td><td><strong>Flow rate (mL\/min)<\/strong><\/td><td><strong>% Mobile Phase A<\/strong><\/td><td><strong>% Mobile Phase B<\/strong><\/td><\/tr><tr><td>0.00<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>7.00<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>7.01<\/td><td>0.4<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>9.00<\/td><td>0.4<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>9.01<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>12.00<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>When labs began testing and validating this method, they determined there was a coelution caused by an analyte not in the method. Due to the regulations, labs are not permitted to change the method parameters but are required to state how\/why there could be an interference, along with how they would rectify it.<\/p>\n\n\n\n<p>Below is an example, provided by Tania Sasaki, PhD, of Confidence Analytics. The unmonitored analyte, CBNA, is coeluting with the critical pair, \u03949-THC and \u03948-THC.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong>\u00a0Coelution of \u03949-THC and CBNA<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-part-3-using-EZLC-cannabinoid-separations-01.jpg\" alt=\"\" title=\"-\"><\/figure>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2:<\/strong>\u00a0Coelution of \u03948-THC and CBNA<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-part-3-using-EZLC-cannabinoid-separations-02.jpg\" alt=\"\" title=\"-\"><\/figure>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>By using <em>EZ<\/em>LC modeling software, it is easy to determine if a method will have coelutions. By changing the concentration of ammonium formate and formic acid you can see all analytes are resolved with the desired USP resolution of 1.3 and above.<\/p>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-part-3-using-EZLC-cannabinoid-separations-03.jpg\" alt=\"conditions interface in the ezlc modeler\" title=\"-\"><\/figure>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0705.png\" alt=\"Pro EZLC Comparison\u2014DCC Method\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0705<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Experimental t<sub>R<\/sub><\/th><th style=\"text-align: center;width: 75px\">Modeled t<sub>R<\/sub><\/th><th style=\"text-align: center;width: 75px\">Difference (sec)<\/th><th style=\"text-align: center;width: 75px\">Run Time Difference (%)<\/th><th style=\"text-align: center;width: 75px\">USP Resolution<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidivarinic acid (CBDVA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/31932-13-5\/Cannabidivarinic acid\" target=\"_blank\" rel=\"noopener\">Cannabidivarinic acid (CBDVA)<\/a><\/td><td class=\"oth\">1.47<\/td><td class=\"oth\">1.32<\/td><td class=\"oth\">9.12<\/td><td class=\"oth\">2.2<\/td><td class=\"oth\"><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidivarin (CBDV)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/24274-48-4\/Cannabidivarin\" target=\"_blank\" rel=\"noopener\">Cannabidivarin (CBDV)<\/a><\/td><td class=\"oth\">1.63<\/td><td class=\"oth\">1.46<\/td><td class=\"oth\">10.26<\/td><td class=\"oth\">2.4<\/td><td class=\"oth\">3.2<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidiolic acid (CBDA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1244-58-2\/Cannabidiolic acid\" target=\"_blank\" rel=\"noopener\">Cannabidiolic acid (CBDA)<\/a><\/td><td class=\"oth\">2.03<\/td><td class=\"oth\">1.81<\/td><td class=\"oth\">13.26<\/td><td class=\"oth\">3.2<\/td><td class=\"oth\">7.1<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabigerolic acid (CBGA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/25555-57-1\/Cannabigerolic acid\" target=\"_blank\" rel=\"noopener\">Cannabigerolic acid (CBGA)<\/a><\/td><td class=\"oth\">2.14<\/td><td class=\"oth\">1.89<\/td><td class=\"oth\">15.24<\/td><td class=\"oth\">3.6<\/td><td class=\"oth\">1.7<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabigerol (CBG)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/25654-31-3\/Cannabigerol\" target=\"_blank\" rel=\"noopener\">Cannabigerol (CBG)<\/a><\/td><td class=\"oth\">2.27<\/td><td class=\"oth\">2.01<\/td><td class=\"oth\">15.78<\/td><td class=\"oth\">3.8<\/td><td class=\"oth\">1.9<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabidiol (CBD)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/13956-29-1\/Cannabidiol\" target=\"_blank\" rel=\"noopener\">Cannabidiol (CBD)<\/a><\/td><td class=\"oth\">2.40<\/td><td class=\"oth\">2.16<\/td><td class=\"oth\">14.64<\/td><td class=\"oth\">3.5<\/td><td class=\"oth\">1.9<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetrahydrocannabivarin (THCV)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/31262-37-0\/Tetrahydrocannabivarin\" target=\"_blank\" rel=\"noopener\">Tetrahydrocannabivarin (THCV)<\/a><\/td><td class=\"oth\">2.59<\/td><td class=\"oth\">2.39<\/td><td class=\"oth\">11.82<\/td><td class=\"oth\">2.8<\/td><td class=\"oth\">2.6<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetrahydrocannabivarinic acid (THCVA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/39986-26-0\/Tetrahydrocannabivarinic acid\" target=\"_blank\" rel=\"noopener\">Tetrahydrocannabivarinic acid (THCVA)<\/a><\/td><td class=\"oth\">3.25<\/td><td class=\"oth\">3.02<\/td><td class=\"oth\">13.92<\/td><td class=\"oth\">3.3<\/td><td class=\"oth\">8.1<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabinol (CBN)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/521-35-7\/Cannabinol\" target=\"_blank\" rel=\"noopener\">Cannabinol (CBN)<\/a><\/td><td class=\"oth\">3.50<\/td><td class=\"oth\">3.21<\/td><td class=\"oth\">17.28<\/td><td class=\"oth\">4.1<\/td><td class=\"oth\">2.6<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabinolic acid (CBNA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2808-39-1\/Cannabinolic acid\" target=\"_blank\" rel=\"noopener\">Cannabinolic acid (CBNA)<\/a><\/td><td class=\"oth\">4.20<\/td><td class=\"oth\">3.89<\/td><td class=\"oth\">18.42<\/td><td class=\"oth\">4.4<\/td><td class=\"oth\">6.6<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for \u03949-Tetrahydrocannabinol (\u03949-THC)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1972-08-3\/\u03949-Tetrahydrocannabinol\" target=\"_blank\" rel=\"noopener\">\u03949-Tetrahydrocannabinol (\u03949-THC)<\/a><\/td><td class=\"oth\">4.39<\/td><td class=\"oth\">4.11<\/td><td class=\"oth\">16.74<\/td><td class=\"oth\">4.0<\/td><td class=\"oth\">1.6<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for \u03948-Tetrahydrocannabinol (\u03948-THC)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/5957-75-5\/\u03948-Tetrahydrocannabinol\" target=\"_blank\" rel=\"noopener\">\u03948-Tetrahydrocannabinol (\u03948-THC)<\/a><\/td><td class=\"oth\">4.54<\/td><td class=\"oth\">4.27<\/td><td class=\"oth\">16.20<\/td><td class=\"oth\">3.9<\/td><td class=\"oth\">1.3<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabicyclol (CBL)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/21366-63-2\/Cannabicyclol\" target=\"_blank\" rel=\"noopener\">Cannabicyclol (CBL)<\/a><\/td><td class=\"oth\">5.21<\/td><td class=\"oth\">4.95<\/td><td class=\"oth\">15.30<\/td><td class=\"oth\">3.6<\/td><td class=\"oth\">5.2<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabichromene (CBC)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/20675-51-8\/Cannabichromene\" target=\"_blank\" rel=\"noopener\">Cannabichromene (CBC)<\/a><\/td><td class=\"oth\">5.47<\/td><td class=\"oth\">5.16<\/td><td class=\"oth\">18.78<\/td><td class=\"oth\">4.5<\/td><td class=\"oth\">1.9<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Tetrahydrocannabinolic acid A (THCA-A)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/23978-85-0\/Tetrahydrocannabinolic acid A\" target=\"_blank\" rel=\"noopener\">Tetrahydrocannabinolic acid A (THCA-A)<\/a><\/td><td class=\"oth\">5.73<\/td><td class=\"oth\">5.43<\/td><td class=\"oth\">17.88<\/td><td class=\"oth\">4.3<\/td><td class=\"oth\">1.7<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Cannabichromenic acid (CBCA)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/185505-15-1\/Cannabichromenic acid\" target=\"_blank\" rel=\"noopener\">Cannabichromenic acid (CBCA)<\/a><\/td><td class=\"oth\">6.45<\/td><td class=\"oth\">6.09<\/td><td class=\"oth\">21.54<\/td><td class=\"oth\">5.1<\/td><td class=\"oth\">4.4<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor ARC-18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9314A62?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0705\" rel=\"noopener\">cat.# 9314A62<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>150 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><td><\/td><td>Cannabinoids acids 7 standard, 1000 \u00b5g\/mL, acetonitrile with 1% DIPEA and 0.05% ascorbic acid (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/34144?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0705\" rel=\"noopener\">cat.# 34144<\/a>)<\/td><\/tr><tr><td><\/td><td>Cannabinoids neutrals 9 standard, 1000 \u00b5g\/mL, P&amp;T methanol, 1 mL\/ampul (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/34132?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0705\" rel=\"noopener\">cat.# 34132<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Acetonitrile<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ppm<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 3 mM ammonium formate, 0.1 % formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.1 % formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>7.00<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>7.01<\/td><td>0.4<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>9.00<\/td><td>0.4<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>9.01<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>12.00<\/td><td>0.4<\/td><td>25<\/td><td>75<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>UV\/Vis @ 228 nm<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow Cell Size:<\/th><td>500 nL <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Waters ACQUITY UPLC H-Class<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Working standard was prepared in a 2 mL, 9 mm amber vial (cat. 21142) by diluting 50 \u00b5L of each standard into 900 \u00b5L acetonitrile and capped with a 9 mm short screw cap (cat. 24497).<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0705.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p>&nbsp;In the coelution above, \u03949-THC is one of the analytes in question. Without adequate separation of \u03949-THC from other analytes in the method, hemp could be misidentified as cannabis. This coelution can also cause inflated THC numbers, leading to potential potency inflation whether intentional or unintentional.<\/p>\n\n\n\n<p>In summary, even if you are not required or do not want to monitor a larger panel of cannabinoids, it is important that during method development you consider all compounds that could be present to ensure accurate identification and quantitative results.<\/p>\n\n\n\n<p>Tune in for the final installment of this series to learn how to harness the full capability of your column.<\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><strong>Resources and Further Reading<\/strong><\/p>\n\n\n\n<p>H.R.5485 &#8211; 115th Congress (2017-2018): Hemp Farming Act of 2018 | congress.gov | library of Congress. (n.d.-a). <a href=\"https:\/\/www.congress.gov\/bill\/115th-congress\/house-bill\/5485\" target=\"_blank\" rel=\"noopener\">https:\/\/www.congress.gov\/bill\/115th-congress\/house-bill\/5485<\/a><\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><strong>Check Out the Full Blog Series in the Related Resources below.<\/strong><\/p>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Products Mentioned<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9314A62\">                                Catalog No. 9314A62                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor ARC-18, 2.7 \u00b5m, 150 x 2.1 mm HPLC\u30ab\u30e9\u30e0<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9314A62\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The third in a four-part series, this blog explores how to ensure nontarget compounds aren&#8217;t interfering with your cannabinoids analysis using the free <em>EZ<\/em>LC chromatogram modeler.<\/p>\n","protected":false},"author":39,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[9],"tags":[],"industries-application":[2263,2282],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[2343],"hf_cat_post":[623],"ppma_author":[459],"class_list":["post-51843","post","type-post","status-publish","format-standard","hentry","category-blogs","industries-application-cannabis-ja","industries-application-medical-recreational-cannabis-ja","resource-technique-liquid-chromatography-ja"],"acf":[],"taxonomy_info":{"category":[{"value":9,"label":"Blogs"}],"industries-application":[{"value":2263,"label":"\u30ab\u30f3\u30ca\u30d3\u30b9"},{"value":2282,"label":"\u533b\u7642\u7528\uff5c\u55dc\u597d\u7528 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