{"id":71339,"date":"2025-09-08T20:06:09","date_gmt":"2025-09-08T20:06:09","guid":{"rendered":"https:\/\/discover.restek.com\/?p=71339"},"modified":"2025-12-04T18:47:31","modified_gmt":"2025-12-04T18:47:31","slug":"japanese-method-development-and-column-selection-how-the-fluorophenyl-phase-provides-the-power-of-hilic-and-reversed-phase-modes-in-one-column","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/articles-ja\/gnar2613-ja\/japanese-method-development-and-column-selection-how-the-fluorophenyl-phase-provides-the-power-of-hilic-and-reversed-phase-modes-in-one-column","title":{"rendered":"\u30e1\u30bd\u30c3\u30c9\u958b\u767a\u3068\u30ab\u30e9\u30e0\u9078\u629e\uff1aFluoroPhenyl \u56fa\u5b9a\u76f8\u306f\u3069\u306e\u3088\u3046\u306bHILIC\u30fb\u9006\u76f8\u306e\u4e21\u30e2\u30fc\u30c9\u3067\u4f5c\u7528\u3059\u308b\u306e\u304b"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">\u6982\u8981<\/h2>\n\n\n\n<p>\u96e3\u3057\u3044\u5206\u96e2\u304c\u3046\u307e\u304f\u3044\u304f\u304b\u3069\u3046\u304b\u306f\u30ab\u30e9\u30e0\u306e\u4fdd\u6301\u3068\u9078\u629e\u6027\u306b\u3088\u3063\u3066\u5927\u304d\u304f\u5de6\u53f3\u3055\u308c\u308b\u305f\u3081\u3001\u30ab\u30e9\u30e0\u9078\u629e\u306f\u30e1\u30bd\u30c3\u30c9\u958b\u767a\u306b\u304a\u3044\u3066\u91cd\u8981\u3067\u3059\u3002\u4e00\u822c\u7684\u306b\u306fC18\u30ab\u30e9\u30e0\u304c\u4f7f\u7528\u3055\u308c\u307e\u3059\u304c\u3001FluoroPhenyl\u30ab\u30e9\u30e0\u306f\u30e1\u30bd\u30c3\u30c9\u958b\u767a\u306b\u5168\u304f\u7570\u306a\u308b\u9078\u629e\u6027\u3092\u3082\u305f\u3089\u3057\u307e\u3059\u3002\u3053\u308c\u306f\u3001FluoroPhenyl\u306b\u8907\u6570\u306e\u4fdd\u6301\u6a5f\u69cb\u304c\u7d44\u307f\u8fbc\u307e\u308c\u3066\u304a\u308a\u3001\u9006\u76f8\u30e2\u30fc\u30c9\u3068\u89aa\u6c34\u6027\u76f8\u4e92\u4f5c\u7528\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\uff08HILIC\uff09\u30e2\u30fc\u30c9\u306e\u4e21\u65b9\u3067\u5229\u7528\u3067\u304d\u308b\u305f\u3081\u3067\u3059\u3002\u3053\u3053\u3067\u306f\u3001\u5869\u57fa\u6027\u5316\u5408\u7269\u3084\u4f4e\u5206\u5b50\u6975\u6027\u5316\u5408\u7269\u306e\u3088\u3046\u306b\u7279\u306bC18\u3067\u5206\u96e2\u304c\u96e3\u3057\u3044\u5316\u5408\u7269\u306b\u5bfe\u3057\u3066\u3001FluoroPhenyl\u304c\u3069\u306e\u3088\u3046\u306b\u5206\u96e2\u3092\u6539\u5584\u3059\u308b\u306e\u304b\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u306f\u3058\u3081\u306b<\/h2>\n\n\n\n<p>C18\u56fa\u5b9a\u76f8\u306f\u3053\u308c\u307e\u3067\u591a\u304f\u306e\u30e9\u30dc\u3067LC\u30ab\u30e9\u30e0\u306e\u4e3b\u5f79\u3092\u62c5\u3063\u3066\u304d\u307e\u3057\u305f\u304c\u3001\u4fdd\u6301\u3068\u9078\u629e\u6027\u304c\u9650\u3089\u308c\u3066\u3044\u308b\u305f\u3081\u3001\u89aa\u6c34\u6027\u5316\u5408\u7269\u306b\u3068\u3063\u3066\u306f\u5fc5\u305a\u3057\u3082\u6700\u826f\u306e\u9078\u629e\u3068\u306f\u3044\u3048\u307e\u305b\u3093\u3002C18\u30ab\u30e9\u30e0\u306f\u3001\u4e3b\u306b\u5206\u6563\u529b\u306b\u3088\u3063\u3066\u5316\u5408\u7269\u3092\u5206\u96e2\u3057\u3001\u758e\u6c34\u6027\u306e\u4fdd\u6301\u3092\u3082\u305f\u3089\u3059\u30a2\u30eb\u30ad\u30eb\u56fa\u5b9a\u76f8\u3092\u6709\u3057\u307e\u3059\u3002\u3053\u306e\u5358\u4e00\u306e\u4fdd\u6301\u6a5f\u69cb\u306f\u591a\u304f\u306e\u9006\u76f8\u5206\u6790\u306e\u57fa\u790e\u3068\u306a\u3063\u3066\u3044\u307e\u3059\u304c\u3001\u6700\u7d42\u7684\u306b\u306fC18\u306e\u7528\u9014\u3092\u3001\u758e\u6c34\u6027\u306b\u57fa\u3065\u3044\u3066\u5206\u96e2\u3057\u3046\u308b\u975e\u6975\u6027\u306a\u5206\u6790\u5bfe\u8c61\u5316\u5408\u7269\u306b\u5236\u9650\u3057\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u5bfe\u7167\u7684\u306b\u3001Restek\u306eFluoroPhenyl\u56fa\u5b9a\u76f8\u3067\u306f\u3001\u5206\u6563\u3001\u7acb\u4f53\u9078\u629e\u6027\u3001\u967d\u30a4\u30aa\u30f3\u4ea4\u63db\u3001\u53cc\u6975\u5b50\u3001\u304a\u3088\u3073\u03c0-\u03c0\u76f8\u4e92\u4f5c\u7528\u3092\u542b\u3080\u8907\u6570\u306e\u4fdd\u6301\u6a5f\u69cb\u304c\u4f5c\u7528\u3057\u307e\u3059\u3002\u3053\u306e\u8907\u5408\u7684\u306a\u4fdd\u6301\u6a5f\u69cb\u306e\u5b58\u5728\u304cFluoroPhenyl\u306bC18\u306e\u4ee3\u308f\u308a\u3068\u306a\u308b\u3001\u3082\u3057\u304f\u306f\u5168\u304f\u7570\u306a\u308b\u9078\u629e\u6027\u3092\u3082\u305f\u3089\u3057\u3001\u4f4e\u5206\u5b50\u6975\u6027\u5316\u5408\u7269\u3084\u5869\u57fa\u6027\u5316\u5408\u7269\u3092\u542b\u3080\u5206\u96e2\u306e\u96e3\u3057\u3044\u5316\u5408\u7269\u306b\u5bfe\u3059\u308b\u5f37\u529b\u306a\u30c4\u30fc\u30eb\u3068\u306a\u308a\u307e\u3059\u3002FluoroPhenyl\u306f\u3001\u30d5\u30a7\u30cb\u30eb\u57fa\u306b\u96fb\u6c17\u9670\u6027\u5ea6\u306e\u5927\u304d\u3044\u30d5\u30c3\u7d20\u539f\u5b50\u3092\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u3001\u72ec\u81ea\u306e\u9078\u629e\u6027\u3092\u5b9f\u73fe\u3057\u3066\u3044\u307e\u3059\uff08\u56f31\uff09\u3002\u3053\u306e\u56fa\u5b9a\u76f8\u69cb\u9020\u306b\u3088\u308a\u3001HILIC\u3068\u9006\u76f8\u306e\u4e21\u30e2\u30fc\u30c9\u3092\u4f7f\u7528\u3067\u304d\u308b\u30df\u30c3\u30af\u30b9\u30e2\u30fc\u30c9\u30ab\u30e9\u30e0\u306e\u6a5f\u80fd\u304c\u751f\u3058\u3001\u30e1\u30bd\u30c3\u30c9\u958b\u767a\u306e\u969b\u306b\u5206\u6790\u5bfe\u8c61\u6210\u5206\u306b\u6700\u5584\u306a\u5206\u96e2\u30e2\u30fc\u30c9\u3092\u9078\u3076\u3068\u3044\u3046\u81ea\u7531\u5ea6\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u3053\u3053\u3067\u306f\u3001\u30e1\u30bd\u30c3\u30c9\u958b\u767a\u3068\u30ab\u30e9\u30e0\u9078\u629e\u3092\u691c\u8a0e\u3057\u3001C18\u3068\u6bd4\u8f03\u3057\u3066FluoroPhenyl\u3067\u9054\u6210\u3067\u304d\u308b\u3088\u308a\u5e83\u7bc4\u56f2\u306a\u5206\u96e2\u306b\u3064\u3044\u3066\u793a\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u79fb\u52d5\u76f8\u306e\u7d44\u6210\u3001\u9178\u5f37\u5ea6\u3084\u9178\u6fc3\u5ea6\u306a\u3069\u306e\u30d1\u30e9\u30e1\u30fc\u30bf\u3092\u5909\u66f4\u3059\u308b\u3053\u3068\u3067\u3001\u3069\u306e\u3088\u3046\u306b\u5206\u96e2\u3092\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3057\u6700\u9069\u5316\u3059\u308b\u304b\u306b\u3064\u3044\u3066\u3082\u89e6\u308c\u307e\u3059\u3002\u3000<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>\u56f3 1:<\/strong>\u00a0Raptor FluoroPhenyl LC\u30ab\u30e9\u30e0\u306e\u4ed5\u69d8<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<figure class=\"wp-block-table\"><table style=\"border-width:1px\"><tbody><tr><td><br><strong><strong>\u56fa\u5b9a\u76f8\u30ab\u30c6\u30b4\u30ea:<\/strong><\/strong><\/td><td>Pentafluorophenyl propyl (L43)<\/td><\/tr><tr><td><strong><strong>\u5b98\u80fd\u57fa\u30bf\u30a4\u30d7<\/strong><\/strong><\/td><td>FluoroPhenyl<\/td><\/tr><tr><td><strong><strong>\u7c92\u5b50:<\/strong><\/strong><\/td><td>2.7 \u00b5m or 5 \u00b5m superficially<br>porous silica<br>(SPP or &#8220;core-shell&#8221;)<\/td><\/tr><tr><td><strong><strong>\u7d30\u5b54\u5f84:<\/strong><\/strong><\/td><td>90 \u00c5<\/td><\/tr><tr><td><strong><strong>\u8868\u9762\u7a4d:<\/strong><\/strong><\/td><td>150 m<sup>2<\/sup>\/g (2.7 \u00b5m)<br>100 m<sup>2<\/sup>&nbsp;(5 \u00b5m)<\/td><\/tr><tr><td><strong><strong>\u63a8\u5968\u4f7f\u7528\u6761\u4ef6:<\/strong><\/strong><\/td><td>pH Range: 2.0\u20138.0<br>Max. Temperature; 80 \u00b0C<br>Max. Pressure: 600 bar (2.7 \u00b5m);<br>400 bar (5 \u00b5m)<\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\"><div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"413\" height=\"577\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2613-01.png\" alt=\"Raptor FluroPhenyl LC column structure\" class=\"wp-image-20692\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2613-01.png 413w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2613-01-215x300.png 215w\" sizes=\"auto, (max-width: 413px) 100vw, 413px\" \/><\/figure>\n<\/div><\/div>\n<\/div>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">\u8907\u6570\u306e\u4fdd\u6301\u6a5f\u69cb\u304cC18\u306b\u4ee3\u308f\u308b\u9078\u629e\u6027\u3092FluoroPhenyl\u30ab\u30e9\u30e0\u3078\u3082\u305f\u3089\u3057\u307e\u3059<\/h2>\n\n\n\n<p>\u52b9\u7387\u7684\u304b\u3064\u52b9\u679c\u7684\u306a\u30e1\u30bd\u30c3\u30c9\u958b\u767a\u306f\u3001\u30ab\u30e9\u30e0\u9078\u629e\u306b\u304b\u304b\u3063\u3066\u3044\u307e\u3059\u3002 \u5206\u6790\u5bfe\u8c61\u6210\u5206\u306b\u6700\u9069\u306a\u30ab\u30e9\u30e0\u3092\u65e9\u3044\u6bb5\u968e\u3067\u9078\u5b9a\u3059\u308b\u3053\u3068\u3067\u3001\u65b0\u3057\u3044\u30e1\u30bd\u30c3\u30c9\u958b\u767a\u306b\u8981\u3059\u308b\u6642\u9593\u3092\u7bc0\u7d04\u3067\u304d\u307e\u3059\u3002C18\u3068\u6bd4\u8f03\u3057\u305f\u5834\u5408\u306eFluoroPhenyl\u306e\u7570\u306a\u308b\u9078\u629e\u6027\u306f\u30011\u672c\u306e\u30ab\u30e9\u30e0\u3067\u8907\u5408\u7684\u306a\u4fdd\u6301\u6a5f\u69cb\u3092\u5229\u7528\u3067\u304d\u3001\u30e1\u30bd\u30c3\u30c9\u958b\u767a\u62c5\u5f53\u8005\u3092\u30b5\u30dd\u30fc\u30c8\u3057\u307e\u3059\u3002FluoroPheny\u306e\u72ec\u7279\u306e\u4fdd\u6301\u7279\u6027\u3092\u89e3\u8aac\u3057\u3001HILIC\u304a\u3088\u3073\u9006\u76f8\u30e2\u30fc\u30c9\u306e\u4e21\u65b9\u306b\u3064\u3044\u3066\u306e\u6027\u80fd\u3092\u8abf\u3079\u308b\u305f\u3081\u306b\u3001\u3044\u304f\u3064\u304b\u5206\u96e2\u304c\u96e3\u3057\u3044\u5316\u5408\u7269\uff08\u56f32\uff09\u3092\u9078\u5b9a\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u5316\u5408\u7269\u306f\u5168\u3066\u5f93\u6765\u306eC18\u56fa\u5b9a\u76f8\u3067\u306f\u4fdd\u6301\u304c\u5f31\u3044\u304b\u5206\u96e2\u304c\u60aa\u3044\u3001\u307e\u305f\u306f\u305d\u306e\u4e21\u65b9\u3067\u3042\u308b\u305f\u3081\u3067\u3059\u3002<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>\u56f3 2:\u00a0<\/strong>Raptor FluoroPhenyl\u30ab\u30e9\u30e0\u306e\u8907\u5408\u7684\u306a\u4fdd\u6301\u30e2\u30fc\u30c9\u3092\u5b9f\u8a3c\u3059\u308b\u305f\u3081\u306b\u7528\u3044\u305f\u30c6\u30b9\u30c8\u5316\u5408\u7269<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"299\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-GNAR2613-02-1024x299.jpg\" alt=\"structures of 4-methylimidazole (4-MEI) and Epimers of Vitamin D3\" class=\"wp-image-20698\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2613-02-1024x299.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2613-02-300x88.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2613-02-768x224.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2613-02-1536x448.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2613-02.jpg 1800w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">\u9006\u76f8\u30e2\u30fc\u30c9\u306b\u304a\u3051\u308b Vitamin D \u7570\u6027\u4f53\u306e\u7acb\u4f53\u9078\u629e\u6027\u306b\u3088\u308b\u4fdd\u6301<\/h3>\n\n\n\n<p>\u6700\u521d\u306e\u4f8b\u3068\u3057\u3066\u300125-Hydroxyvitamin D3 \u3068\u305d\u306e C3\u30a8\u30d4\u30de\u30fc\u306e\u5206\u6790\u306b\u3064\u3044\u3066\u8a55\u4fa1\u3092\u884c\u3044\u307e\u3057\u305f\u3002 \u3053\u306e\u5206\u96e2\u306f\u4e16\u754c\u4e2d\u306e\u81e8\u5e8a\u691c\u67fb\u30e9\u30dc\u3067\u95a2\u5fc3\u304c\u9ad8\u307e\u3063\u3066\u3044\u307e\u3059\u3002 25-Hydroxyvitamin D3\u306f\u3001\u30d3\u30bf\u30df\u30f3D\u4ee3\u8b1d\u7570\u5e38\u306e\u5224\u5b9a\u306b\u7528\u3044\u3089\u308c\u308b\u91cd\u8981\u306a\u30d0\u30a4\u30aa\u30de\u30fc\u30ab\u30fc\u3067\u3059\u3002 C3\u30a8\u30d4\u30de\u30fc (3-epi-25-hydroxyvitamin D3) \u306f\u3001\u751f\u7406\u6d3b\u6027\u304c\u306f\u308b\u304b\u306b\u4f4e\u3044\u305f\u3081\u3001\u500b\u3005\u306b\u6e2c\u5b9a\u3057\u306a\u3051\u308c\u3070\u30d0\u30a4\u30aa\u30de\u30fc\u30ab\u30fc\u5024\u3092\u904e\u5927\u8a55\u4fa1\u3059\u308b\u539f\u56e0\u3068\u306a\u308a\u307e\u3059\u3002 \u3053\u308c\u3089\u306e\u5316\u5408\u7269\u306f\u30a2\u30a4\u30bd\u30d0\u30ea\u30c3\u30af\u3067\u3042\u308b\u305f\u3081\u3001\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u5206\u96e2\u306f\u6b63\u78ba\u306a\u751f\u4f53\u4e2d\u6fc3\u5ea6\u6e2c\u5b9a\u306b\u4e0d\u53ef\u6b20\u3067\u3059\u3002 \u56f33\u306b\u793a\u3059\u3088\u3046\u306b\u3001\u9006\u76f8\u30e2\u30fc\u30c9\u3067FluoroPhenyl\u3092\u7528\u3044\u305f\u5834\u5408\u3001\u308f\u305a\u304b5\u5206\u3067 25-Hydroxyvitamin D3\u3068\u305d\u306e C3\u30a8\u30d4\u30de\u30fc\u3092\u5b8c\u5168\u5206\u96e2\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u5bfe\u7167\u7684\u306b\u3001C18\u30ab\u30e9\u30e0\u306f\u6eb6\u51fa\u6642\u9593\u304c3\u500d\u3082\u304b\u304b\u3063\u3066\u3082\u3001\u5341\u5206\u306a\u5206\u96e2\u306f\u5f97\u3089\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u3053\u306e\u4f8b\u306b\u304a\u3044\u3066\u3001\u30ab\u30e9\u30e0\u9078\u629e\u306f\u3001\u30e1\u30bd\u30c3\u30c9\u958b\u767a\u306b\u304a\u3044\u3066\u660e\u3089\u304b\u306b\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u307e\u3059\u3002\u3064\u307e\u308a\u3001FluoroPhenyl\u306e\u7acb\u4f53\u7684\u306b\u5b89\u5b9a\u306a(\u30ea\u30b8\u30c3\u30c8\u306a)\u69cb\u9020\u306f\u3001C18\u30ab\u30e9\u30e0\u306e\u758e\u6c34\u6027\u4fdd\u6301\u3067\u5f97\u3089\u308c\u308b\u3088\u308a\u3082\u3001\u7acb\u4f53\u9078\u629e\u6027\u3092\u5897\u3057\u3001\u672c\u8cea\u7684\u306b\u3088\u308a\u591a\u304f\u306e\u5206\u96e2\u80fd\u304c\u5f97\u3089\u308c\u308b\u7d50\u679c\u3068\u306a\u308a\u307e\u3057\u305f\u3002<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>\u56f3 3:<\/strong>\u00a0\u7acb\u4f53\u9078\u629e\u7684\u306a\u4fdd\u6301\u529b\u3067 C3\u30a8\u30d4\u30de\u30fc\u3068\u751f\u7406\u6d3b\u6027\u578b\u3092\u5206\u96e2\u3059\u308b\u305f\u3081\u3001FluoroPhenyl\u3092\u7528\u3044\u305fVitamin D\u5206\u6790\u306f\u3088\u308a\u6b63\u78ba\u3067\u3059\u3002<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<p><strong>Raptor FluoroPhenyl<\/strong><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0644.png\" alt=\"25-Hydroxyvitamin D2\/D3 and 3-Epi-25-Hydroxyvitamin D2\/D3 on Raptor FluoroPhenyl by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0644<\/p><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor FluoroPhenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9319A1E?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0644\" rel=\"noopener\">cat.# 9319A1E<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 3 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water:methanol (50:50)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>25-50 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.6<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>4.00<\/td><td>0.6<\/td><td>15<\/td><td>85<\/td><\/tr><tr><td>4.10<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>5.00<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>5.01<\/td><td>0.6<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>7.00<\/td><td>0.6<\/td><td>25<\/td><td>75<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-related-skus\"><h4>Related SKUs<\/h4><ul><li>9319A1E<\/li><\/ul><\/div><div class=\"chromatogram-related-searches\"><h4>Related Searches<\/h4><ul><li>epimer<\/li><li>vitamin D<\/li><li>vitamin D epimer<\/li><li>vitamin D metabolite<\/li><\/ul><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0644.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p><strong>Raptor ARC-18<\/strong><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0649.png\" alt=\"25-Hydroxyvitamin D2\/D3 and 3-Epi-25-Hydroxyvitamin D2\/D3 on Raptor\u2122 ARC-18 by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0649<\/p><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor ARC-18  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9314A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0649\" rel=\"noopener\">cat.# 9314A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water:methanol (50:50)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>25-50 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>4.00<\/td><td>0.5<\/td><td>20<\/td><td>80<\/td><\/tr><tr><td>12.00<\/td><td>0.5<\/td><td>20<\/td><td>80<\/td><\/tr><tr><td>12.10<\/td><td>0.5<\/td><td>25<\/td><td>75<\/td><\/tr><tr><td>14.00<\/td><td>0.5<\/td><td>25<\/td><td>75<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-related-skus\"><h4>Related SKUs<\/h4><ul><li>9314A12<\/li><\/ul><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0649.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">HILIC\u30e2\u30fc\u30c9\u306b\u304a\u3051\u308b\u4f4e\u5206\u5b50\u6975\u6027\u5316\u5408\u7269\u306e\u967d\u30a4\u30aa\u30f3\u4ea4\u63db\u4f5c\u7528<\/h3>\n\n\n\n<p>\u6b21\u306e\u4f8b\u3067\u306fFluoroPhenyl\u306e\u967d\u30a4\u30aa\u30f3\u4ea4\u63db\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u3068\u4f4e\u5206\u5b50\u6975\u6027\u5316\u5408\u7269\u306e\u4fdd\u6301\u306b\u304a\u3051\u308b\u305d\u306e\u52b9\u679c\u3092\u793a\u3057\u307e\u3059\u30024-Methylimidazole(4-MEI)\u306f\u98df\u54c1\u3084\u98f2\u6599\u3067\u30ab\u30e9\u30e1\u30eb\u8272\u7d20\u3068\u3057\u3066\u7528\u3044\u3089\u308c\u308b\u6dfb\u52a0\u7269\u3067\u3059\u3002\u4ed6\u306e\u6975\u6027\u4f4e\u5206\u5b50\u5316\u5408\u7269\u3068\u540c\u69d8\u306b\u3001\u975e\u6975\u6027\u56fa\u5b9a\u76f8\u3067\u306e\u4fdd\u6301\u304c\u9650\u3089\u308c\u3066\u3044\u308b\u305f\u3081\u3001\u6a19\u6e96\u7684\u306a\u9006\u76f8\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3068C18\u30ab\u30e9\u30e0\u3092\u7528\u3044\u305f\u5206\u6790\u306f\u56f0\u96e3\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u9178\u6027\u6eb6\u6db2\u4e2d\u306b\u304a\u3044\u30664-MEI\u306f\u6b63\u96fb\u8377\u3092\u5e2f\u3073\u308b\u305f\u3081\u967d\u30a4\u30aa\u30f3\u4ea4\u63db\u306b\u975e\u5e38\u306b\u9069\u3057\u3066\u304a\u308a \u3001HILIC\u6761\u4ef6\u4e0b\u3067\u5bb9\u6613\u306b FluoroPhenyl\u3078\u4fdd\u6301\u3055\u308c\u307e\u3059\u3002\u3053\u308c\u306f\uff15\u3064\u306e\u30d5\u30c3\u7d20\u539f\u5b50\u306e\u96fb\u5b50\u6c42\u5f15\u6027\u3067\u56fa\u5b9a\u76f8\u304c\u308f\u305a\u304b\u306b\u8ca0\u306b\u8377\u96fb\u3059\u308b\u305f\u3081\u3067\u3059\u3002\u56f34\u306b\u793a\u3059\u3088\u3046\u306b\u3001\u79fb\u52d5\u76f8\u4e2d\u306e\u30a2\u30bb\u30c8\u30cb\u30c8\u30ea\u30eb\u6bd4\u7387\u304c\u9ad8\u3044\u3068\u304d\u3001\u967d\u30a4\u30aa\u30f3\u4ea4\u63db\u4f5c\u7528\u304c\u512a\u4f4d\u306b\u306a\u308a4-MEI\u306f\u975e\u5e38\u306b\u826f\u304f\u4fdd\u6301\u3055\u308c\u307e\u3059\u3002\u9006\u76f8\u6761\u4ef6\u4e0b\u306b\u304a\u3044\u3066\u3001FluoroPhenyl \u306e\u4fdd\u6301\u306f\u5206\u6563\u529b\u304c\u512a\u4f4d\u306b\u306a\u308a\u30014-MEI\u306f\u307b\u3068\u3093\u3069\u4fdd\u6301\u3055\u308c\u307e\u305b\u3093\u3002FluoroPhenyl\u56fa\u5b9a\u76f8\u306f\u9006\u76f8\u3068HILIC\u306e\u4e21\u30e2\u30fc\u30c9\u3067\u5206\u96e2\u6027\u80fd\u3092\u8a55\u4fa1\u3059\u308b\u3068\u3044\u3046\u81ea\u7531\u5ea6\u304c\u3042\u308a\u3001\u76ee\u7684\u5316\u5408\u7269\u306b\u5bfe\u3057\u3066\u3069\u3061\u3089\u304c\u6700\u3082\u52b9\u679c\u7684\u304b\u3092\u78ba\u8a8d\u3057\u305f\u3046\u3048\u3067\u30e1\u30bd\u30c3\u30c9\u3092\u958b\u767a\u30fb\u6c7a\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>\u56f3 4:<\/strong>\u00a04-MEI \u306e\u3088\u3046\u306a\u4f4e\u5206\u5b50\u6975\u6027\u5316\u5408\u7269\u306f FluoroPhenyl \u30ab\u30e9\u30e0\u306b\u304a\u3051\u308b\u9006\u76f8\u6761\u4ef6\u3067\u306f\u4fdd\u6301\u306f\u56f0\u96e3\u3067\u3059\u304c\u3001HILIC\u6761\u4ef6\u3067\u306f\u5bb9\u6613\u306b\u4fdd\u6301\u3055\u308c\u307e\u3059\u3002<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0562.png\" alt=\"HILIC-Like Retention of 4-Methylimidazole\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FF0562<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 4-Methylimidazole (4-MEI)\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/822-36-6\/4-Methylimidazole\" target=\"_blank\" rel=\"noopener\">4-Methylimidazole (4-MEI)<\/a><\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor FluoroPhenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9319A52?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_FF0562\" rel=\"noopener\">cat.# 9319A52<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Acetonitrile<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>100 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>5 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td>Water, 0.1% formic acid:acetonitrile, 0.1% formic acid<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow:<\/th><td>0.6 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ff0562.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">\u79fb\u52d5\u76f8\u6761\u4ef6\u5909\u66f4\u306b\u3088\u308b\u4fdd\u6301\u3068\u9078\u629e\u6027\u306e\u5fae\u8abf\u6574<\/h2>\n\n\n\n<p>\u524d\u8ff0\u306e\u901a\u308a\u3001\u52b9\u679c\u7684\u306a\u30e1\u30bd\u30c3\u30c9\u958b\u767a\u306f\u614e\u91cd\u306a\u30ab\u30e9\u30e0\u9078\u5b9a\u306b\u304b\u304b\u3063\u3066\u3044\u307e\u3059\u3002\u305d\u3057\u3066FluoroPhenyl\u56fa\u5b9a\u76f8\u3067\u306f\u8907\u5408\u7684\u306a\u4fdd\u6301\u30e2\u30fc\u30c9\u306b\u3088\u308a\u5358\u4e00\u306e\u4fdd\u6301\u30e2\u30fc\u30c9\u3057\u304b\u6301\u305f\u306a\u3044C18\u30ab\u30e9\u30e0\u3088\u308a\u3082\u5927\u304d\u306a\u81ea\u7531\u5ea6\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u3053\u306e\u9805\u3067\u306f\u79fb\u52d5\u76f8\u7d44\u6210\u3084\u9178\u306e\u5f37\u5ea6\u3001\u9178\u6fc3\u5ea6\u3092\u5909\u66f4\u3059\u308b\u3053\u3068\u3067\u5206\u96e2\u3092\u6700\u9069\u5316\u3059\u308b\u65b9\u6cd5\u3092\u691c\u8a0e\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u5b9f\u9a13\u3067\u306fFluoroPhenyl\u306e\u591a\u69d8\u306a\u4fdd\u6301\u6319\u52d5\u3092\u5b9f\u8a3c\u3059\u308b\u305f\u3081\u306b\u3044\u304f\u3064\u304b\u306e\u4e00\u822c\u7684\u306a\u85ac\u7269\u3092\u9078\u5b9a\u3057\u307e\u3057\u305f\uff08\u56f35\uff09\u3002\u3053\u308c\u3089\u306e\u30c6\u30b9\u30c8\u5316\u5408\u7269\u306f\u5869\u57fa\u6027\u5316\u5408\u7269\u3001\u5f31\u9178\u6027\u5316\u5408\u7269\u3001\u4e2d\u6027\u5316\u5408\u7269\u3067\u3042\u308a\u3001\u89aa\u6c34\u6027\u304a\u3088\u3073\u5869\u57fa\u6027\u5316\u5408\u7269\u306b\u5bfe\u3059\u308b FluoroPhenyl \u306e\u9078\u629e\u6027\u3068\u89aa\u548c\u6027\u306e\u9055\u3044\u3092\u660e\u3089\u304b\u306b\u3057\u307e\u3059\u3002\u3000<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>\u56f3 5:<\/strong>\u00a0Raptor FluoroPhenyl \u30ab\u30e9\u30e0\u306e\u8907\u6570\u306e\u4fdd\u6301\u30e2\u30fc\u30c9\u3092\u5b9f\u8a3c\u3059\u308b\u305f\u3081\u306b\u7528\u3044\u305f\u30c6\u30b9\u30c8\u5316\u5408\u7269<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"602\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/\/figure-article-GNAR2613-05-1024x602.jpg\" alt=\"Structures of atenolol (base), phenytoin (weak acid), trenbolone (neutral), testosterone (neutral), nortriptyline (base), amitriptyline (base), and celecoxib (weak acid)\" class=\"wp-image-20704\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2613-05-1024x602.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2613-05-300x177.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2613-05-768x452.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2613-05-1536x904.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2613-05.jpg 1800w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n<\/div><\/div><\/div>\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\">\u79fb\u52d5\u76f8\u7d44\u6210\u306e\u5f71\u97ff<\/h3>\n\n\n\n<p>\u30e1\u30bd\u30c3\u30c9\u958b\u767a\u306b\u304a\u3044\u3066FluoroPhenyl\u30ab\u30e9\u30e0\u3092\u9078\u629e\u3059\u308b\u4e3b\u306a\u5229\u70b9\u306e1\u3064\u306f\u3001HILIC\u3068\u9006\u76f8\u306e\u4e21\u30e2\u30fc\u30c9\u3067\u4f7f\u7528\u3067\u304d\u308b\u3053\u3068\u3067\u3059\u3002\u79fb\u52d5\u76f8\u7d44\u6210\u3092\u5909\u3048\u308b\u3053\u3068\u3067\u3001\u4fdd\u6301\u3068\u9078\u629e\u6027\u306b\u5909\u5316\u304c\u8d77\u3053\u308a\u307e\u3059\u3002\u9006\u76f8\u6761\u4ef6\u4e0b\u3067\u306f\u3001\u5206\u6790\u5bfe\u8c61\u7269\u306f\u5206\u6563\u3068\u6975\u6027\u306e\u76f8\u4e92\u4f5c\u7528\u306b\u3088\u3063\u3066FluoroPhenyl\u306b\u4fdd\u6301\u3055\u308c\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001 \u79fb\u52d5\u76f8\u4e2d\u306e\u6709\u6a5f\u6eb6\u5a92\u6bd4\u7387\u304c\u5f90\u3005\u306b\u9ad8\u304f\u306a\u308b\u306b\u3064\u308c\u3066\u3001 \u967d\u30a4\u30aa\u30f3\u4ea4\u63db\u3067\u306e\u4fdd\u6301\u304c\u512a\u4f4d\u306b\u306a\u308b\u3053\u3068\u3067FluoroPhenyl\u56fa\u5b9a\u76f8\u306f\u6b63\u96fb\u8377\u3092\u3082\u3064\u5869\u57fa\u6027\u5316\u5408\u7269\u3092\u7279\u7570\u7684\u306b\u4fdd\u6301\u3059\u308b\u3088\u3046\u306b\u306a\u308a\u3001\u4e2d\u6027\u5316\u5408\u7269\u3068\u9178\u6027\u5316\u5408\u7269\u306f\u30ab\u30e9\u30e0\u30dc\u30a4\u30c9\u4ed8\u8fd1\u306b\u6eb6\u51fa\u3057\u307e\u3059\uff08\u56f36\uff09\u3002\u8907\u6570\u306e\u4fdd\u6301\u6a5f\u69cb\u304c\u4f5c\u7528\u3059\u308bFluoroPhenyl\u306e\u5b9f\u529b\u306f\u3001\u79fb\u52d5\u76f8\u7d44\u6210\u3092\u6c34\u306e\u6bd4\u7387\u304c\u9ad8\u3044\u7d44\u6210\uff08\u9006\u76f8\uff09\u304b\u3089\u6709\u6a5f\u6eb6\u5a92\u306e\u6bd4\u7387\u304c\u9ad8\u3044\u7d44\u6210(HILIC)\u3078\u5909\u66f4\u3057\u3066\u3044\u304f\u306b\u3064\u308c\u3001\u4e00\u90e8\u306e\u5869\u57fa\u6027\u5316\u5408\u7269\u3067\u89b3\u5bdf\u3055\u308c\u308b\u300cU\u5b57\u578b\u300d\u306e\u4fdd\u6301\u6319\u52d5\u306b\u3088\u3063\u3066\u660e\u78ba\u306b\u5b9f\u8a3c\u3055\u308c\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u4fdd\u6301\u529b\u3068\u9078\u629e\u6027\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u3060\u3051\u3067\u306a\u304f\u3001\u30a8\u30ec\u30af\u30c8\u30ed\u30b9\u30d7\u30ec\u30fc\u30a4\u30aa\u30f3\u5316\u8cea\u91cf\u5206\u6790(ESI-MS)\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u3001\u6709\u6a5f\u6eb6\u5a92\u6bd4\u7387\u304c\u9ad8\u3044HILIC\u79fb\u52d5\u76f8\u3078\u5909\u66f4\u3059\u308b\u3068\u3001\u8131\u6eb6\u5a92\u548c\u52b9\u7387\u304c\u5411\u4e0a\u3057\u3001\u611f\u5ea6\u306b\u5927\u304d\u306a\u5f71\u97ff\u3092\u4e0e\u3048\u307e\u3059\u3002\u8868I\u306f\u56f36\u3067\u5206\u6790\u3057\u305f\u5869\u57fa\u6027\u5316\u5408\u7269\u306e\u30d4\u30fc\u30af\u5f37\u5ea6\u3092\u6bd4\u8f03\u3057\u305f\u3082\u306e\u3067\u3001\u611f\u5ea6\u5897\u52a0\u304c\u30ce\u30eb\u30c8\u30ea\u30d7\u30c1\u30ea\u30f3\u3068\u30a2\u30df\u30c8\u30ea\u30d7\u30c1\u30ea\u30f3\u306e\u4e21\u65b9\u3067\u89b3\u5bdf\u3055\u308c\u305f\u3053\u3068\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002\uff08\u9006\u76f8\u30e2\u30fc\u30c9\u3067\u306f\u4fdd\u6301\u3055\u308c\u306a\u304b\u3063\u305f\u5316\u5408\u7269\u304cHILIC\u30e2\u30fc\u30c9\u3067\u4fdd\u6301\u3055\u308c\u308b\u3088\u3046\u306b\u306a\u3063\u305f\u305f\u3081\u3067\u3042\u308a\u3001\u30a2\u30c6\u30ce\u30ed\u30fc\u30eb\u306e\u611f\u5ea6\u306f\u5909\u308f\u3063\u3066\u3044\u307e\u305b\u3093\u3002\uff09<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>\u56f3 6:<\/strong>\u00a0\u79fb\u52d5\u76f8\u7d44\u6210\u3092\u9006\u76f8 (40% B) \u304b\u3089HILIC (90% B) \u6761\u4ef6\u306b\u5909\u66f4\u3059\u308b\u3068\u3001\u4fdd\u6301\u3068\u9078\u629e\u6027\u306e\u5909\u5316\u3092\u793a\u3059U\u5b57\u578b\u306e\u4fdd\u6301\u6319\u52d5\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u3053\u308c\u306f\u56fa\u5b9a\u76f8\u306b\u8907\u6570\u306e\u4fdd\u6301\u30e1\u30ab\u30cb\u30ba\u30e0\u304c\u5b58\u5728\u3059\u308b\u305f\u3081\u306b\u751f\u3058\u307e\u3059\u3002<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0570.png\" alt=\"Affecting Selectivity on FluoroPhenyl Poster Figure 4: Dual Mode Retention\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0570<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">Precursor Ion<\/th><th style=\"text-align: center;width: 50px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Atenolol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/29122-68-7\/Atenolol\" target=\"_blank\" rel=\"noopener\">Atenolol<\/a><\/td><td class=\"oth\">267.3<\/td><td class=\"oth\">145.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Phenytoin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-41-0\/Phenytoin\" target=\"_blank\" rel=\"noopener\">Phenytoin<\/a><\/td><td class=\"oth\">253.0<\/td><td class=\"oth\">181.7<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Trenbolone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/10161-34-9\/Trenbolone\" target=\"_blank\" rel=\"noopener\">Trenbolone<\/a><\/td><td class=\"oth\">271.2<\/td><td class=\"oth\">199.2<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Testosterone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/58-22-0\/Testosterone\" target=\"_blank\" rel=\"noopener\">Testosterone<\/a><\/td><td class=\"oth\">289.2<\/td><td class=\"oth\">109.1<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nortriptyline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/72-69-5\/Nortriptyline\" target=\"_blank\" rel=\"noopener\">Nortriptyline<\/a><\/td><td class=\"oth\">264.1<\/td><td class=\"oth\">91.1<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Amitriptyline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-48-6\/Amitriptyline\" target=\"_blank\" rel=\"noopener\">Amitriptyline<\/a><\/td><td class=\"oth\">278.1<\/td><td class=\"oth\">202.1<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Celecoxib\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/169590-42-5\/Celecoxib\" target=\"_blank\" rel=\"noopener\">Celecoxib<\/a><\/td><td class=\"oth\">382.2<\/td><td class=\"oth\">362.0<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor FluoroPhenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9319A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0570\" rel=\"noopener\">cat.# 9319A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Custom mix<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ng\/mL  (150 ng\/mL for phenytoin)<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water + 0.1% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile + 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.8<\/td><\/tr><tr><td>20<\/td><td>0.8<\/td><\/tr><\/tbody><\/table><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Max Pressure:<\/th><td>400 bar<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0570.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><strong>\u8868 I:<\/strong>\u00a0\u6709\u6a5f\u6eb6\u5a92\u6bd4\u7387\u306e\u9ad8\u3044 HILIC\u79fb\u52d5\u76f8\u3068ESI-MS\u306e\u7d44\u307f\u5408\u308f\u305b\u308b\u306b\u3088\u308b\u5927\u5e45\u306a\u611f\u5ea6\u5411\u4e0a<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table style=\"border-width:1px\"><thead><tr><th class=\"has-text-align-left\" data-align=\"left\"><strong>Analyte<\/strong><\/th><th class=\"has-text-align-left\" data-align=\"left\"><strong>RP Intensity (40% B, Isocratic)<\/strong><\/th><th class=\"has-text-align-left\" data-align=\"left\"><strong>HILIC Intensity (90% B, Isocratic)<\/strong><\/th><th class=\"has-text-align-left\" data-align=\"left\"><strong>Increase in Response<\/strong><\/th><\/tr><\/thead><tbody><tr><td class=\"has-text-align-left\" data-align=\"left\">Atenolol<\/td><td class=\"has-text-align-left\" data-align=\"left\">5.0e4<\/td><td class=\"has-text-align-left\" data-align=\"left\">5.0e4<\/td><td class=\"has-text-align-left\" data-align=\"left\">\u2013<\/td><\/tr><tr><td class=\"has-text-align-left\" data-align=\"left\">Nortriptyline<\/td><td class=\"has-text-align-left\" data-align=\"left\">7.5e4<\/td><td class=\"has-text-align-left\" data-align=\"left\">1.3e5<\/td><td class=\"has-text-align-left\" data-align=\"left\">73%<\/td><\/tr><tr><td class=\"has-text-align-left\" data-align=\"left\">Amitriptyline<\/td><td class=\"has-text-align-left\" data-align=\"left\">8.5e4<\/td><td class=\"has-text-align-left\" data-align=\"left\">1.7e5<\/td><td class=\"has-text-align-left\" data-align=\"left\">100%<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\">\u79fb\u52d5\u76f8\u4e2d\u306e\u9178\u306e\u5f37\u5ea6\u3068\u6fc3\u5ea6\u306e\u5f71\u97ff<\/h3>\n\n\n\n<p>\u4e0a\u8ff0\u306e\u3088\u3046\u306b\u3001\u79fb\u52d5\u76f8\u306e\u6c34\u3068\u6709\u6a5f\u6eb6\u5a92\u306e\u6bd4\u7387\u3092\u52a0\u6e1b\u3059\u308b\u3053\u3068\u306fFluoroPhenyl\u306e\u4fdd\u6301\u3068\u9078\u629e\u6027\u306b\u8457\u3057\u3044\u5909\u5316\u3092\u3082\u305f\u3089\u3057\u3001\u30e1\u30bd\u30c3\u30c9\u958b\u767a\u3067\u7279\u5b9a\u306e\u5206\u96e2\u306e\u6700\u9069\u5316\u3092\u304a\u3053\u306a\u3044\u305f\u3044\u5834\u5408\u306b\u3001\u4fa1\u5024\u306e\u3042\u308b\u624b\u6bb5\u3092\u63d0\u4f9b\u3057\u307e\u3059\u3002\u3055\u3089\u306b\u3001\u9178\u6027\u79fb\u52d5\u76f8\u3067\u306f\u9178\u306e\u6fc3\u5ea6\u3060\u3051\u3067\u306f\u306a\u304f\u5f37\u5ea6\u3082\u30ab\u30c1\u30aa\u30f3\u4ea4\u63db\u306e\u4fdd\u6301\u30e1\u30ab\u30cb\u30ba\u30e0\u306b\u4f5c\u7528\u3057\u3001\u5206\u96e2\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u56f37\u306b\u793a\u3059\u3088\u3046\u306b\u3001\u30ae\u9178\u306a\u3069\u5f37\u9178\u3092\u542b\u3080\u79fb\u52d5\u76f8\u3092\u7528\u3044\u308b\u3068\u3001\u9162\u9178\u306a\u3069\u3088\u308a\u5f31\u9178\u3092\u540c\u3058\u6fc3\u5ea6\u3067\u4f7f\u7528\u3057\u305f\u540c\u6761\u4ef6\u306e\u5206\u6790\u3068\u6bd4\u8f03\u3057\u3066\u3001\u5869\u57fa\u6027\u5316\u5408\u7269\u306e\u4fdd\u6301\u304c\u6e1b\u5c11\u3057\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u3069\u3061\u3089\u306e\u5834\u5408\u3082\u4e2d\u6027\u3068\u5f31\u9178\u6027\u5316\u5408\u7269\u306e\u4fdd\u6301\u306b\u306f\u307b\u3068\u3093\u3069\u5909\u5316\u304c\u3042\u308a\u307e\u305b\u3093\u3002\u540c\u69d8\u306b\u3001\u4f7f\u7528\u3059\u308b\u9178\u306e\u6fc3\u5ea6\u3082FluoroPhenyl\u306e\u30ab\u30c1\u30aa\u30f3\u4ea4\u63db\u4fdd\u6301\u30e1\u30ab\u30cb\u30ba\u30e0\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u5834\u5408\u3001\u3088\u308a\u9ad8\u3044\u9178\u6fc3\u5ea6\u306e\u79fb\u52d5\u76f8\u3092\u4f7f\u7528\u3059\u308b\u3068\u3001\u9006\u76f8\u30e2\u30fc\u30c9\u3068 HILIC\u30e2\u30fc\u30c9\u306e\u4e21\u65b9\u3067\u3001\u4e2d\u6027\u5316\u5408\u7269\u304a\u3088\u3073\u5f31\u9178\u6027\u5316\u5408\u7269\u306e\u4fdd\u6301\u306e\u5909\u5316\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u306a\u304c\u3089\u3001\u5869\u57fa\u6027\u5316\u5408\u7269\u306e\u4fdd\u6301\u3092\u6e1b\u3089\u3059\u3053\u3068\u304c\u3067\u304d\u307e\u3059\uff08\u56f38\uff09\u3002&nbsp;<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>\u56f3 7:<\/strong>\u9006\u76f8\u30e2\u30fc\u30c9\u306b\u304a\u3051\u308bFluoroPhenyl\u306e\u4fdd\u6301\u3068\u9078\u629e\u6027\u306b\u5bfe\u3059\u308b\u9178\u5f37\u5ea6\u306e\u5f71\u97ff<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0573.png\" alt=\"Affecting Selectivity on FluoroPhenyl Poster Figure 6: Effects of Acid Strength\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0573<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">Precursor Ion<\/th><th style=\"text-align: center;width: 50px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Atenolol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/29122-68-7\/Atenolol\" target=\"_blank\" rel=\"noopener\">Atenolol<\/a><\/td><td class=\"oth\">267.3<\/td><td class=\"oth\">145.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Phenytoin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-41-0\/Phenytoin\" target=\"_blank\" rel=\"noopener\">Phenytoin<\/a><\/td><td class=\"oth\">253.0<\/td><td class=\"oth\">181.7<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Trenbolone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/10161-34-9\/Trenbolone\" target=\"_blank\" rel=\"noopener\">Trenbolone<\/a><\/td><td class=\"oth\">271.2<\/td><td class=\"oth\">199.2<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Testosterone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/58-22-0\/Testosterone\" target=\"_blank\" rel=\"noopener\">Testosterone<\/a><\/td><td class=\"oth\">289.2<\/td><td class=\"oth\">109.1<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nortriptyline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/72-69-5\/Nortriptyline\" target=\"_blank\" rel=\"noopener\">Nortriptyline<\/a><\/td><td class=\"oth\">264.1<\/td><td class=\"oth\">91.1<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Amitriptyline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-48-6\/Amitriptyline\" target=\"_blank\" rel=\"noopener\">Amitriptyline<\/a><\/td><td class=\"oth\">278.1<\/td><td class=\"oth\">202.1<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Celecoxib\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/169590-42-5\/Celecoxib\" target=\"_blank\" rel=\"noopener\">Celecoxib<\/a><\/td><td class=\"oth\">382.2<\/td><td class=\"oth\">362.0<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor FluoroPhenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9319A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0573\" rel=\"noopener\">cat.# 9319A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Custom mix<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ng\/mL  (150 ng\/mL for phenytoin)<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water + 0.1% acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile + 0.1% acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.8<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>10.00<\/td><td>0.8<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>10.01<\/td><td>0.8<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>12.00<\/td><td>0.8<\/td><td>95<\/td><td>5<\/td><\/tr><\/tbody><\/table><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Max Pressure:<\/th><td>400 bar<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0573.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>\u56f3 8:<\/strong>\u00a0FluoroPhenyl\u306e\u4fdd\u6301\u3068\u9078\u629e\u6027\u306b\u5bfe\u3059\u308b\u9178\u5f37\u5ea6\u306e\u5f71\u97ff<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<p><strong>Reversed-Phase Mode<\/strong><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0571.png\" alt=\"Affecting Selectivity on FluoroPhenyl Poster Figure 5A: Effects of Acid Concentration RP Mode\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0571<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">Precursor Ion<\/th><th style=\"text-align: center;width: 50px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Atenolol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/29122-68-7\/Atenolol\" target=\"_blank\" rel=\"noopener\">Atenolol<\/a><\/td><td class=\"oth\">267.3<\/td><td class=\"oth\">145.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Phenytoin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-41-0\/Phenytoin\" target=\"_blank\" rel=\"noopener\">Phenytoin<\/a><\/td><td class=\"oth\">253.0<\/td><td class=\"oth\">181.7<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Trenbolone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/10161-34-9\/Trenbolone\" target=\"_blank\" rel=\"noopener\">Trenbolone<\/a><\/td><td class=\"oth\">271.2<\/td><td class=\"oth\">199.2<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Testosterone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/58-22-0\/Testosterone\" target=\"_blank\" rel=\"noopener\">Testosterone<\/a><\/td><td class=\"oth\">289.2<\/td><td class=\"oth\">109.1<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nortriptyline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/72-69-5\/Nortriptyline\" target=\"_blank\" rel=\"noopener\">Nortriptyline<\/a><\/td><td class=\"oth\">264.1<\/td><td class=\"oth\">91.1<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Amitriptyline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-48-6\/Amitriptyline\" target=\"_blank\" rel=\"noopener\">Amitriptyline<\/a><\/td><td class=\"oth\">278.1<\/td><td class=\"oth\">202.1<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Celecoxib\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/169590-42-5\/Celecoxib\" target=\"_blank\" rel=\"noopener\">Celecoxib<\/a><\/td><td class=\"oth\">382.2<\/td><td class=\"oth\">362.0<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor FluoroPhenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9319A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0571\" rel=\"noopener\">cat.# 9319A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Custom mix<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ng\/mL  (150 ng\/mL for phenytoin)<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water + x% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile + x% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.8<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>10.00<\/td><td>0.8<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>10.01<\/td><td>0.8<\/td><td>95<\/td><td>5<\/td><\/tr><tr><td>12.00<\/td><td>0.8<\/td><td>95<\/td><td>5<\/td><\/tr><\/tbody><\/table><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Max Pressure:<\/th><td>400 bar<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0571.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n\n\n<p><strong>HILIC Mode<\/strong><\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0572.png\" alt=\"Affecting Selectivity on FluoroPhenyl Poster Figure 5B: Effects of Acid Concentration HILIC Mode\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0572<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">Precursor Ion<\/th><th style=\"text-align: center;width: 50px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Atenolol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/29122-68-7\/Atenolol\" target=\"_blank\" rel=\"noopener\">Atenolol<\/a><\/td><td class=\"oth\">267.3<\/td><td class=\"oth\">145.1<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Phenytoin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/57-41-0\/Phenytoin\" target=\"_blank\" rel=\"noopener\">Phenytoin<\/a><\/td><td class=\"oth\">253.0<\/td><td class=\"oth\">181.7<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Trenbolone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/10161-34-9\/Trenbolone\" target=\"_blank\" rel=\"noopener\">Trenbolone<\/a><\/td><td class=\"oth\">271.2<\/td><td class=\"oth\">199.2<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Testosterone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/58-22-0\/Testosterone\" target=\"_blank\" rel=\"noopener\">Testosterone<\/a><\/td><td class=\"oth\">289.2<\/td><td class=\"oth\">109.1<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nortriptyline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/72-69-5\/Nortriptyline\" target=\"_blank\" rel=\"noopener\">Nortriptyline<\/a><\/td><td class=\"oth\">264.1<\/td><td class=\"oth\">91.1<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Amitriptyline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-48-6\/Amitriptyline\" target=\"_blank\" rel=\"noopener\">Amitriptyline<\/a><\/td><td class=\"oth\">278.1<\/td><td class=\"oth\">202.1<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Celecoxib\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/169590-42-5\/Celecoxib\" target=\"_blank\" rel=\"noopener\">Celecoxib<\/a><\/td><td class=\"oth\">382.2<\/td><td class=\"oth\">362.0<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor FluoroPhenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9319A12?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_GN0572\" rel=\"noopener\">cat.# 9319A12<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>100 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Custom mix<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Methanol<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>50 ng\/mL  (150 ng\/mL for phenytoin)<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, x% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, x% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.8<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>10.00<\/td><td>0.8<\/td><td>80<\/td><td>20<\/td><\/tr><tr><td>10.01<\/td><td>0.8<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>12.00<\/td><td>0.8<\/td><td>5<\/td><td>95<\/td><\/tr><\/tbody><\/table><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Max Pressure:<\/th><td>400 bar<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0572.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">\u307e\u3068\u3081<\/h2>\n\n\n\n<p>\u30e1\u30bd\u30c3\u30c9\u958b\u767a\u304c\u6210\u529f\u3059\u308b\u304b\u3069\u3046\u304b\u306f\u3001\u30ab\u30e9\u30e0\u9078\u629e\u304c\u5927\u304d\u306a\u9375\u3068\u306a\u308a\u307e\u3059\u3002C18\u30ab\u30e9\u30e0\u304c\u7b2c\u4e00\u9078\u629e\u3067\u3042\u308b\u4e00\u65b9\u3067\u3001\u5206\u6790\u5bfe\u8c61\u7269\u306e\u5316\u5b66\u7684\u6027\u8cea\u3092\u56fa\u5b9a\u76f8\u306e\u7279\u6027\u3068\u3068\u3082\u306b\u614e\u91cd\u306b\u691c\u8a0e\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u306a\u305c\u306a\u3089\u3001FluoroPhenyl\u56fa\u5b9a\u76f8\u306f\u8907\u6570\u306e\u4fdd\u6301\u30e1\u30ab\u30cb\u30ba\u30e0\uff08\u5206\u6563\u3001\u7acb\u4f53\u9078\u629e\u6027\u3001\u967d\u30a4\u30aa\u30f3\u4ea4\u63db\u3001\u53cc\u6975\u5b50\u3001\u304a\u3088\u3073\u03c0-\u03c0\uff09\u3092\u6709\u3059\u308b\u305f\u3081\u3001\u4e3b\u306b\u5206\u6563\u4f5c\u7528\u3057\u304b\u5229\u7528\u3057\u306a\u3044C18\u3068\u6bd4\u8f03\u3057\u3066FluoroPheny\u306f\u7570\u306a\u308b\u9078\u629e\u6027\u3092\u63d0\u4f9b\u3059\u308b\u304b\u3089\u3067\u3059\u3002\u3053\u3053\u3067\u793a\u3057\u305f\u3088\u3046\u306b\u3001\u9006\u76f8\u3068 HILIC\u30e2\u30fc\u30c9\u306e\u4e21\u65b9\u3067\u4f7f\u7528\u3067\u304d\u308b FluoroPhenyl\u306e\u8907\u6570\u306e\u4fdd\u6301\u30e1\u30ab\u30cb\u30ba\u30e0\u306f\u3001C18\u3067\u306f\u9054\u6210\u3067\u304d\u306a\u3044\u5206\u96e2\u3092\u6210\u3057\u9042\u3052\u307e\u3059\u3002\u3055\u3089\u306b\u3001\u79fb\u52d5\u76f8\u7d44\u6210\u3001\u9178\u306e\u5f37\u5ea6\u3001\u306a\u3089\u3073\u306b\u9178\u6fc3\u5ea6\u3092\u5909\u66f4\u3059\u308b\u3053\u3068\u3067\u5206\u96e2\u3092\u3055\u3089\u306b\u5411\u4e0a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u3001\u3088\u308a\u591a\u304f\u306e\u4fdd\u6301\u3068\u9078\u629e\u6027\u304c\u5229\u7528\u53ef\u80fd\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Products Mentioned<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9319A1E\">                                Catalog No. 9319A1E                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor FluoroPhenyl, 2.7 \u00b5m, 100 x 3.0 mm HPLC\u30ab\u30e9\u30e0<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9319A1E\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9319A52\">                                Catalog No. 9319A52                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor FluoroPhenyl, 2.7 \u00b5m, 50 x 2.1 mm HPLC\u30ab\u30e9\u30e0<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9319A52\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9319A12\">                                Catalog No. 9319A12                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor FluoroPhenyl, 2.7 \u00b5m, 100 x 2.1 mm HPLC\u30ab\u30e9\u30e0<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9319A12\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9314A12\">                                Catalog No. 9314A12                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor ARC-18, 2.7 \u00b5m, 100 x 2.1 mm HPLC\u30ab\u30e9\u30e0<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9314A12\">\u88fd\u54c1\u60c5\u5831\u3092\u898b\u308b<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" 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