{"id":76492,"date":"2013-02-27T00:00:00","date_gmt":"2013-02-27T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/?p=76492"},"modified":"2026-01-22T16:05:50","modified_gmt":"2026-01-22T16:05:50","slug":"japanese-building-up-pressure-on-hplc","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/ja\/blogs-ja\/gnbl5472-ja\/japanese-building-up-pressure-on-hplc","title":{"rendered":"HPLC\u306e\u5727\u529b\u304c\u4e0a\u304c\u3063\u305f\uff1f"},"content":{"rendered":"\n<p>The original English blog&nbsp;was posted on February 28th, 2013 by Nancy Schwartz<\/p>\n\n\n\n<p>\u79c1\u305f\u3061\u306f\u7686\u3001\u904e\u53bb\u306bHPLC\u306e\u5727\u529b\u30a8\u30e9\u30fc\u3092\u7d4c\u9a13\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u3053\u3067\u306f\u305d\u306e\u89e3\u6d88\u306e\u305f\u3081\u6700\u826f\u306e\u63d0\u6848\u3092\u3044\u305f\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"170\" height=\"169\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-building-up-pressure-on-hplc-01.jpg\" alt=\"\" class=\"wp-image-77509\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-building-up-pressure-on-hplc-01.jpg 170w, https:\/\/discover.restek.com\/wp-content\/uploads\/blog-building-up-pressure-on-hplc-01-150x150.jpg 150w\" sizes=\"auto, (max-width: 170px) 100vw, 170px\" \/><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<p><strong>\u57fa\u672c\u30eb\u30fc\u30eb\u306e\u8a2d\u5b9a<\/strong>&nbsp;\u2013 \u554f\u984c\u3092\u660e\u78ba\u306b\u3057\u3001\u672c\u5f53\u306b\u554f\u984c\u304c\u3042\u308b\u306e\u304b\u5224\u5225\u3059\u308b\u306e\u306b\u5f79\u7acb\u3061\u307e\u3059\u3002<\/p>\n\n\n\n<p><strong>1.\u3000\u8a2d\u5b9a\u3055\u308c\u305f\u5727\u529b\u4e0a\u9650\u3092\u78ba\u8a8d\u3059\u308b\u3002<\/strong>\u30b7\u30b9\u30c6\u30e0\u306e\u5727\u529b\u4e0a\u9650\u3068\u30e1\u30bd\u30c3\u30c9\u306e\u5727\u529b\u4e0a\u9650\u306e\u8a2d\u5b9a\u306b\u6ce8\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u3053\u306e\u60c5\u5831\u306b\u3064\u3044\u3066\u306f\u6a5f\u5668\u306e\u88fd\u9020\u30e1\u30fc\u30ab\u30fc\u306b\u554f\u3044\u5408\u308f\u305b\u308b\u304b\u63d0\u4f9b\u3055\u308c\u3066\u3044\u308b\u30aa\u30da\u30ec\u30fc\u30b7\u30e7\u30f3\u30de\u30cb\u30e5\u30a2\u30eb\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002\u5727\u529b\u4e0a\u9650\u3092\u8a2d\u5b9a\u3059\u308b\u969b\u306f\u88fd\u9020\u30e1\u30fc\u30ab\u30fc\u306e\u63a8\u5968\u9650\u754c\u3092\u8d85\u3048\u306a\u3044\u3088\u3046\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u6c4e\u7528HPLC\uff08UHPLC\u3067\u306a\u3044\u3082\u306e\uff09\u306e\u80cc\u5727\u4e0a\u9650\u306f\u901a\u5e38 5800 \u2013 7000 psi (400 \u2013 500 bar \/ 40 \u2013 50 MPa)\u306e\u7bc4\u56f2\u3067\u3042\u308a\u3001\u3053\u308c\u306f\u30ab\u30e9\u30e0\u306e\u90e8\u6750\u3084\uff08\u5168\u591a\u5b54\u6027\u306e\uff09\u5145\u586b\u5264\u306e\u6027\u8cea\u306b\u5247\u3057\u305f\u3082\u306e\u3067\u3059\u3002\u7269\u7406\u7684\u306a\u5727\u529b\u9650\u754c\u306f\u901a\u5e38\u30d0\u30eb\u30d6\u3001\u30dd\u30f3\u30d7\u3084\u4ed6\u306e\u69cb\u6210\u7269\u306e\u898f\u683c\u306b\u3088\u3063\u3066\u8a2d\u5b9a\u3055\u308c\u3001\u6f0f\u308c\u304c\u751f\u3058\u308b\u307e\u3067\u306e\u8010\u4e45\u6027\u306b\u5927\u304d\u304f\u95a2\u4fc2\u3057\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u30e1\u30bf\u30ce\u30fc\u30eb\u3084\u6c34\u3068\u3044\u3063\u305f\u6eb6\u5a92\u306f\u3082\u3068\u3082\u3068\u9ad8\u3044\u80cc\u5727\u3068\u306a\u308b\u3053\u3068\u306b\u3054\u7559\u610f\u304f\u3060\u3055\u3044\u3002\u4e00\u822c\u7684\u306b\u30013 \u00b5m\u3082\u3057\u304f\u306f5 \u00b5m\u306e\u5168\u591a\u5b54\u6027\u30ab\u30e9\u30e0\u306b6000 psi\u4ee5\u4e0a\u306e\u5727\u529b\u3092\u304b\u3051\u308b\u3053\u3068\u306f\u304a\u3059\u3059\u3081\u3057\u307e\u305b\u3093\u3002\uff08\u3053\u308c\u306fRaptor(\u30b3\u30a2\u30b7\u30a7\u30eb\u3001SPP)\u3084\u7c92\u5b50\u5f841.9 \u00b5m\u306eUHPLC\u30ab\u30e9\u30e0\u3092\u9664\u304d\u307e\u3059\uff09<\/p>\n\n\n\n<p><strong>2.\u3000\u6700\u826f\u306e\u5206\u96e2\u304c\u5f97\u3089\u308c\u308b\u6d41\u91cf\u3092\u8a2d\u5b9a\u3059\u308b\u3002<\/strong>\u4e00\u65b9\u3067\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306e\u5206\u89e3\u80fd\u306f\u3088\u308a\u5b9f\u7528\u7684\u306a\u9650\u754c\u3067\u3042\u308a\u3001\u5b89\u5b9a\u3067\u79d1\u5b66\u7684\u306a\u30c7\u30fc\u30bf\u304c\u3082\u305f\u3089\u3059\u7d50\u679c\u3068\u898b\u306a\u3059\u3053\u3068\u304c\u3067\u304d\u308b\u304b\u306b\u3088\u3063\u3066\u6c7a\u5b9a\u3055\u308c\u307e\u3059\u3002\u4ee5\u4e0b\u306e\u8868\u3092\u4f7f\u3063\u3066\u3001\u30ab\u30e9\u30e0\u306e\u5145\u586b\u5264\u6bce\u306b\u6700\u826f\u306e\u5206\u96e2\u304c\u5f97\u3089\u308c\u308b\u6700\u9069\u306a\u6d41\u91cf\u3092\u6c7a\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u308c\u306f\u307e\u305f\u3001\u30a2\u30bb\u30c8\u30cb\u30c8\u30ea\u30eb\u3084\u30e1\u30bf\u30ce\u30fc\u30eb\u3067\u793a\u3055\u308c\u308b\u30ab\u30e9\u30e0\u5727\u3092\u4e88\u6e2c\u3059\u308b\u3053\u3068\u3082\u3067\u304d\u307e\u3059\u3002\u500b\u3005\u306e\u30ab\u30e9\u30e0\u306e\u5b9f\u969b\u306e\u5727\u529b\u306f\u82e5\u5e72\u5909\u5316\u3057\u3001\u7528\u9014\u306b\u3088\u3063\u3066\u306f\u591a\u5c11\u4e0a\u304c\u308b\u3053\u3068\u304c\u3042\u308b\u3053\u3068\u306b\u7559\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u307e\u305f\u3001\u5727\u529b\u8868\u793a\u5024\u306f\u30b7\u30b9\u30c6\u30e0\u81ea\u4f53\u306e\u672c\u6765\u306e\u80cc\u5727\u3082\u542b\u3093\u3060\u5168\u4f53\u7684\u306a\u5727\u529b\u3092\u53cd\u6620\u3057\u3066\u3044\u308b\u3053\u3068\u3082\u899a\u3048\u3066\u304a\u3044\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p><strong>3.\u3000\u30ab\u30e9\u30e0\u306a\u3057\u3067\u88c5\u7f6e\u306e\u5727\u529b\u3092\u30e2\u30cb\u30bf\u30fc\u3059\u308b\u3002<\/strong>\u30ab\u30e9\u30e0\u3092\u53d6\u308a\u4ed8\u3051\u308b\u524d\u306b\u53e4\u3044\u30ab\u30e9\u30e0\u306e\u4ee3\u308f\u308a\u306b\u30e6\u30cb\u30aa\u30f3\u30b3\u30cd\u30af\u30bf\u30fc\u3067\u63a5\u7d9a\u3057\u3001\u88c5\u7f6e\u30921\u30b5\u30a4\u30af\u30eb\u52d5\u304b\u3057\u307e\u3059\u3002\u3053\u306e\u69cb\u6210\u3067\u306e\u88c5\u7f6e\u5727\u529b\u3092\u8a18\u9332\u3057\u3001\u53c2\u7167\u306e\u305f\u3081\u30d5\u30a1\u30a4\u30eb\u306b\u4fdd\u7ba1\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u5727\u529b\u306e\u4e0a\u6607\u306f\u6d41\u8def\u306b\u8a70\u307e\u308a\u3084\u62b5\u6297\u304c\u751f\u3058\u305f\u3053\u3068\u3092\u793a\u3057\u307e\u3059\u306e\u3067\u3001\u8a18\u9332\u306f\u6642\u3005\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u3053\u306e\u3088\u3046\u306a\u5909\u5316\u3092\u898b\u3064\u3051\u308b\u3053\u3068\u3067\u5727\u529b\u8d85\u904e\u306b\u3088\u308b\u30a8\u30e9\u30fc\u3084\u8d77\u3053\u308a\u3046\u308b\u5371\u967a\u3092\u9632\u3050\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<p><strong>4.\u3000\u65b0\u3057\u3044\u30ab\u30e9\u30e0\u306e\u5727\u529b\u3092\u8a18\u9332\u3057\u307e\u3059\u3002<\/strong>\u5206\u6790\u306e\u305f\u3081\u306b\u30ab\u30e9\u30e0\u3092\u53d6\u308a\u4ed8\u3051\u5206\u6790\u7cfb\u304c\u5b89\u5b9a\u3057\u305f\u3089\u3059\u3050\u306b\u3001\u79fb\u52d5\u76f8\u3084\u6d41\u91cf\u6761\u4ef6\u3068\u4f75\u305b\u3066\u5727\u529b\u3082\u8a18\u9332\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u3053\u308c\u306f\u30ab\u30e9\u30e0\u306e\u52a3\u5316\u3092\u77e5\u308b\u4e0a\u3067\u76ee\u5b89\u3068\u306a\u308a\u307e\u3059\u3002\u306a\u304a\u3001\u4ee5\u4e0b\u306e\u8868\u306f\u5168\u591a\u5b54\u8cea\u306e\u30ab\u30e9\u30e0\u306b\u306e\u307f\u5f53\u3066\u306f\u307e\u308b\u3082\u306e\u3067\u3001\u30b3\u30a2\u30b7\u30a7\u30eb(SPP)\u3067\u3042\u308bRaptor\u306f\u5bfe\u8c61\u3068\u306a\u308a\u307e\u305b\u3093\u3002<\/p>\n\n\n\n<figure class=\"wp-block-table table table-bordered\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>Column Inner Diameter (mm)<\/strong><\/td><td><strong>Column Length (mm)<\/strong><\/td><td><strong>Particle Diameter (\u00b5m)<\/strong><\/td><td><strong>Optimal Flow rate (mL\/min)<\/strong><\/td><td><strong>Predicted Pressure for ACN (psi) @30C<\/strong><\/td><td><strong>Predicted Pressure for MeOH (psi) @30C<\/strong><\/td><\/tr><tr><td>2.1<\/td><td>30<\/td><td>3<\/td><td>0.31<\/td><td>527<\/td><td>838<\/td><\/tr><tr><td>2.1<\/td><td>30<\/td><td>5<\/td><td>0.2<\/td><td>122<\/td><td>195<\/td><\/tr><tr><td>2.1<\/td><td>50<\/td><td>3<\/td><td>0.31<\/td><td>879<\/td><td>1396<\/td><\/tr><tr><td>2.1<\/td><td>50<\/td><td>5<\/td><td>0.2<\/td><td>204<\/td><td>324<\/td><\/tr><tr><td>2.1<\/td><td>100<\/td><td>3<\/td><td>0.31<\/td><td>1757<\/td><td>2792<\/td><\/tr><tr><td>2.1<\/td><td>100<\/td><td>5<\/td><td>0.2<\/td><td>408<\/td><td>649<\/td><\/tr><tr><td>3.0<\/td><td>50<\/td><td>3<\/td><td>0.65<\/td><td>903<\/td><td>1434<\/td><\/tr><tr><td>3.0<\/td><td>50<\/td><td>5<\/td><td>0.4<\/td><td>200<\/td><td>318<\/td><\/tr><tr><td>3.0<\/td><td>100<\/td><td>3<\/td><td>0.65<\/td><td>1806<\/td><td>2869<\/td><\/tr><tr><td>3.0<\/td><td>100<\/td><td>5<\/td><td>0.4<\/td><td>400<\/td><td>636<\/td><\/tr><tr><td>3.0<\/td><td>150<\/td><td>3<\/td><td>0.65<\/td><td>2708<\/td><td>4303<\/td><\/tr><tr><td>3.0<\/td><td>150<\/td><td>5<\/td><td>0.4<\/td><td>600<\/td><td>953<\/td><\/tr><tr><td>4.6<\/td><td>50<\/td><td>3<\/td><td>1.5<\/td><td>886<\/td><td>1408<\/td><\/tr><tr><td>4.6<\/td><td>50<\/td><td>5<\/td><td>1<\/td><td>213<\/td><td>338<\/td><\/tr><tr><td>4.6<\/td><td>100<\/td><td>3<\/td><td>1.5<\/td><td>1772<\/td><td>2816<\/td><\/tr><tr><td>4.6<\/td><td>100<\/td><td>5<\/td><td>1<\/td><td>425<\/td><td>676<\/td><\/tr><tr><td>4.6<\/td><td>150<\/td><td>3<\/td><td>1.5<\/td><td>2658<\/td><td>4224<\/td><\/tr><tr><td>4.6<\/td><td>150<\/td><td>5<\/td><td>1<\/td><td>638<\/td><td>1014<\/td><\/tr><tr><td>4.6<\/td><td>200<\/td><td>3<\/td><td>1.5<\/td><td>4431<\/td><td>7040<\/td><\/tr><tr><td>4.6<\/td><td>200<\/td><td>5<\/td><td>1<\/td><td>1063<\/td><td>1690<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><strong>\u539f\u56e0\u3092\u63a2\u3057\u51fa\u3059<\/strong>&nbsp;\u2013&nbsp;\u3057\u3070\u3089\u304f\u30ab\u30e9\u30e0\u3092\u4f7f\u7528\u3057\u3001\u65b0\u54c1\u306e\u6642\u3068\u6bd4\u8f03\u3057\u3066\uff08\u4e0a\u8a18#4\uff09\u5727\u529b\u4e0a\u6607\u306b\u6c17\u304c\u3064\u3044\u305f\u3089\u3001\u3069\u3053\u306b\u539f\u56e0\u304c\u3042\u308b\u306e\u304b\u78ba\u8a8d\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u308c\u306f\u30ab\u30e9\u30e0\u304b\u3082\u3057\u308c\u306a\u3044\u3057\u305d\u3046\u3067\u306a\u3044\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"410\" height=\"173\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/blog-building-up-pressure-on-hplc-02.gif\" alt=\"\" class=\"wp-image-77515\" title=\"-\"><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<p><strong>A.<\/strong>\u3000 \u30ab\u30e9\u30e0\u306e\u51fa\u53e3\u306e\u63a5\u7d9a\u3092\u5916\u3057\u3001\u51fa\u53e3\u306e\u30c1\u30e5\u30fc\u30d6\u3092\u76f4\u63a5\u5ec3\u6db2\u6e9c\u3081\u304b\u30d3\u30fc\u30ab\u30fc\u306b\u5165\u308c\u307e\u3059\u3002\u30dd\u30f3\u30d7\u3092\u30aa\u30f3\u306b\u3057\u3066\u5727\u529b\u3092\u89b3\u5bdf\u3001\u8a18\u9332\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u307e\u305f\u3001\u30ab\u30e9\u30e0\u304b\u3089\u51fa\u3066\u304d\u305f\u3088\u3046\u306b\u898b\u3048\u308b\u5fae\u7c92\u5b50\u3084\u8272\u306e\u3064\u3044\u305f\u7269\u8cea\u306b\u3082\u6c17\u3092\u914d\u308a\u307e\u3059\u3002\u30dd\u30f3\u30d7\u306f20\u5206\u3082\u3057\u304f\u306f\u76ee\u8996\u53ef\u80fd\u306a\u7c92\u5b50\u304c\u51fa\u3066\u3053\u306a\u304f\u306a\u308b\u307e\u3067\u52d5\u304b\u3057\u7d9a\u3051\u3066\u3001\u518d\u5ea6\u5727\u529b\u306b\u5909\u5316\u304c\u306a\u3044\u304b\u78ba\u8a8d\u3057\u5024\u3092\u8a18\u9332\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u3082\u3057\u76ee\u8996\u53ef\u80fd\u306a\u7c92\u5b50\u304c20\u5206\u4ee5\u4e0a\u51fa\u7d9a\u3051\u308b\u5834\u5408\u3001\u30ab\u30e9\u30e0\u3092\u53d6\u308a\u5916\u3057\u3066\u4ea4\u63db\u3057\u307e\u3059\u3002\u3053\u306e\u539f\u56e0\u3092\u5224\u65ad\u3059\u308b\u305f\u3081\u306b\u306f\u30c6\u30af\u30cb\u30ab\u30eb\u30b5\u30fc\u30d3\u30b9\u306b\u3054\u9023\u7d61\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p><strong>B.\u3000<\/strong>\u30b9\u30c6\u30c3\u30d7A\u3067\u30ab\u30e9\u30e0\u3092\u5916\u3057\u305f\u5f8c\u3001\u76ee\u8996\u3067\u304d\u308b\u7c92\u5b50\u3082\u306a\u304f\u5727\u529b\u304c\u901f\u3084\u304b\u306b\u901a\u5e38\u7bc4\u56f2\uff08\u4e0a\u8a18#4\uff09\u306b\u623b\u3063\u305f\u3089\u3001\u691c\u51fa\u5668\u306b\u7d9a\u304f\u914d\u7ba1\u3084\u30d0\u30eb\u30d6\u306e\u3069\u3053\u304b\u3001\u3042\u308b\u3044\u306f\u691c\u51fa\u5668\u306e\u30d5\u30ed\u30fc\u30bb\u30eb\u3068\u3044\u3063\u305f\u30ab\u30e9\u30e0\u4ee5\u964d\u306e\u6d41\u8def\u306b\u4f55\u3089\u304b\u306e\u62b5\u6297\u304c\u3042\u308a\u307e\u3059\u3002\u5fc5\u8981\u306b\u5fdc\u3058\u3066\u88c5\u7f6e\u30e1\u30fc\u30ab\u30fc\u306e\u63a8\u5968\u3059\u308b\u65b9\u6cd5\u3067\u30e9\u30a4\u30f3\u30c1\u30e5\u30fc\u30d6\u3092\u4ea4\u63db\u3082\u3057\u304f\u306f\u30d5\u30ed\u30fc\u30bb\u30eb\u3092\u6383\u9664\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u3059\u3079\u3066\u306e\u30d5\u30a3\u30c3\u30c6\u30a3\u30f3\u30b0\u3068\u30d0\u30eb\u30d6\u304c\u9069\u5207\u306b\u7d44\u307f\u7acb\u3066\u3089\u308c\u3066\u3044\u308b\u304b\u3068\u30e9\u30a4\u30f3\u30c1\u30e5\u30fc\u30d6\u304c\u6298\u308c\u66f2\u304c\u3063\u3066\u3044\u306a\u3044\u304b\u78ba\u8a8d\u3057\u307e\u3059\u3002\u7de9\u885d\u5869\u304c\u6790\u51fa\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u3092\u3075\u307e\u3048\u3066\u3001\u5fc5\u8981\u3067\u3042\u308c\u3070\u30d1\u30fc\u30c4\u3092\u6d17\u6d44\u3042\u308b\u3044\u306f\u4ea4\u63db\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p><strong>C.<\/strong>\u3000\u30b9\u30c6\u30c3\u30d7A\u306e\u64cd\u4f5c\u5f8c\u3001\u7c92\u5b50\u306e\u6d41\u51fa\u304c\u78ba\u8a8d\u3055\u308c\u305a\u3001\u5727\u529b\u306e\u9855\u8457\u306a\u4f4e\u4e0b\u304c\u306a\u3044\u5834\u5408\u3001\u30ab\u30e9\u30e0\u3092\u53d6\u308a\u5916\u3057\u4ee3\u308f\u308a\u306b\u30e6\u30cb\u30aa\u30f3\u30b3\u30cd\u30af\u30bf\u30fc\u3092\u63a5\u7d9a\u3057\u307e\u3059\u3002\u30dd\u30f3\u30d7\u3092\u52d5\u304b\u3057\u5b89\u5b9a\u3057\u305f\u3089\u4e0a\u8ff0#3\u3067\u8a18\u9332\u3057\u3066\u3044\u308b\u521d\u671f\u72b6\u614b\u3068\u5727\u529b\u3092\u6bd4\u8f03\u3057\u307e\u3059\u3002\u88c5\u7f6e\u306e\u5727\u529b\u304c\u4e0a\u8ff0#3\u306e\u904e\u53bb\u306e\u305d\u308c\u3068\u8fd1\u3044\u306e\u3067\u3042\u308c\u3070\u3001\u5727\u529b\u306e\u4e0a\u6607\u306f\u30ab\u30e9\u30e0\u7531\u6765\u3067\u3042\u308a\u3001\u304a\u305d\u3089\u304f\u5165\u53e3\u5074\u306e\u30d5\u30ea\u30c3\u30c8\u304c\u5fae\u7c92\u5b50\u3067\u76ee\u8a70\u307e\u308a\u3092\u8d77\u3053\u3057\u3066\u3044\u308b\u306e\u3067\u3057\u3087\u3046\u30023 \u00b5m\u30685 \u00b5m\u7c92\u5b50\u306e\u5834\u5408\u3001\u5168\u591a\u5b54\u6027\u30ab\u30e9\u30e0\u306b\u9650\u308a\u307e\u3059\u304c\u3001\u79fb\u52d5\u76f8\u306730\u5206\u306e\u9006\u6d17\u3092\u8a66\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u3053\u306e\u3068\u304d\u5ec3\u6db2\u6e9c\u3081\u306b\u76f4\u63a5\u9001\u6db2\u3057\u3066\u304f\u3060\u3055\u3044\uff08\u9006\u6d17\u306f1.9 \u00b5m\u306eUHPLC\u30ab\u30e9\u30e0\u3084Raptor\u30b7\u30ea\u30fc\u30ba\u306b\u306f\u304a\u3059\u3059\u3081\u3057\u307e\u305b\u3093\u306e\u3067\u3054\u7559\u610f\u304f\u3060\u3055\u3044\uff09\u5727\u529b\u304c\u307e\u3060\u4e0a\u6607\u3057\u3066\u3044\u308b\u3088\u3046\u306a\u3089\u3001\u30c6\u30af\u30cb\u30ab\u30eb\u30ce\u30fc\u30c8\u300cRestek Liquid Chromatography (LC) Columns \u2014 Cleaning Recommendations\u300d\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u308b\u3088\u3046\u306b\u69d8\u3005\u306a\u6975\u6027\u306e\u4e00\u9023\u306e\u6eb6\u5a92\u3092\u4f7f\u7528\u3057\u3066\u9006\u6d17\u3092\u7e70\u308a\u8fd4\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u4f7f\u7528\u3057\u3066\u3044\u308b\u79fb\u52d5\u76f8\u304c\u7de9\u885d\u5869\u3092\u542b\u3093\u3067\u3044\u308b\u5834\u5408\u3001\u6700\u521d\u306b\u7de9\u885d\u5869\u3001\u9178\u3084\u305d\u306e\u307b\u304b\u306e\u8abf\u6574\u5264\u3092\u542b\u307e\u306a\u3044\u3001\u6c34\u306e\u5272\u5408\u304c\u9ad8\u3044\u6eb6\u6db2\uff08\u5178\u578b\u7684\u306aC18\u306a\u308960-90 %\uff09\u3067\u6d17\u6d44\u3057\u307e\u3059\u3002\u5869\u7d20\u7cfb\u6eb6\u5a92\u3092\u4f7f\u7528\u3059\u308b\u969b\uff0cPEEK\u6750\u8cea\u306f\u5c11\u3005\u81a8\u6f64\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002\u3053\u306e\u624b\u9806\u306b\u3064\u3044\u3066\u3054\u4e0d\u660e\u306a\u70b9\u304c\u3042\u308a\u307e\u3057\u305f\u3089\u3001\u30c6\u30af\u30cb\u30ab\u30eb\u30b5\u30fc\u30d3\u30b9\u3078\u3054\u9023\u7d61\u304f\u3060\u3055\u3044\u3002\u3082\u3057\u9006\u6d17\u3092\u884c\u3063\u3066\u3082\u80cc\u5727\u304c\u4e0b\u304c\u3089\u306a\u3044\u306a\u3089\u3001\u5341\u4e2d\u516b\u4e5d\u3001\u5fae\u7c92\u5b50\u3082\u3057\u304f\u306f\u6d3b\u6027\u7269\u8cea\u304c\u4e0d\u53ef\u9006\u7684\u306b\u30ab\u30e9\u30e0\u306b\u7d50\u5408\u3057\u3001\u4ea4\u63db\u304c\u5fc5\u8981\u306b\u306a\u308b\u3067\u3057\u3087\u3046\u3002\u540c\u69d8\u306b\u3053\u308c\u307e\u3067\u306e\u624b\u9806\u306b\u5f93\u3063\u30661.9 \u00b5m\u306eUHPLC\u30ab\u30e9\u30e0\u3084Raptor\u30ab\u30e9\u30e0\u3092\u9806\u65b9\u5411\u306b\u3066\u6d17\u6d44\u3057\u3066\u3082\u554f\u984c\u304c\u89e3\u6c7a\u3057\u306a\u304b\u3063\u305f\u5834\u5408\u3001\u30ab\u30e9\u30e0\u306f\u5bff\u547d\u3092\u8fce\u3048\u3066\u3044\u307e\u3059\u306e\u3067\u4ea4\u63db\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n\n\n\n<p><strong>D.<\/strong>\u3000\u3082\u3057\u30ab\u30e9\u30e0\u3092\u63a5\u7d9a\u305b\u305a\u306b\u88c5\u7f6e\u306e\u5727\u529b\u3092\u78ba\u8a8d\u3057\uff08\u524d\u8ff0#3\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\uff09\u3001\u4ee5\u524d\u30ab\u30e9\u30e0\u306a\u3057\u3067\u78ba\u8a8d\u3057\u305f\u5727\u529b\u3088\u308a\u9ad8\u3044\u5834\u5408\u3001\u88c5\u7f6e\u306b\u8a70\u307e\u308a\u3084\u62b5\u6297\u304c\u3042\u308a\u307e\u3059\u3002HPLC\u306e\u64cd\u4f5c\u30de\u30cb\u30e5\u30a2\u30eb\u3092\u53c2\u7167\u3059\u308b\u304b\u3001\u88c5\u7f6e\u306e\u88fd\u9020\u4f1a\u793e\u306b\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<div 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