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PFAS Analysis by DIN 38407-42 for Water, Wastewater, and Sludge

03 Oct 2025

feature EVAR4299
  • Sample Matrix: water, wastewater, and sludge
  • Sample Preparation: WAX solid-phase extraction
  • Detection Technique: LC-MS/MS

Determination of selected polyfluorinated compounds (PFC) in water-Method using high-performance liquid chromatography and mass spectrometric detection (HPLC/MS-MS) after solid-liquid extraction

DIN 38407-42 is used for the analysis of 10 PFAS in water, wastewater, and sludge. It is not available from Restek but may be purchased from other sources. The full analyte list and recommended products for DIN 38407-42 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.

Analyte List for DIN 38407-42 for PFAS Analysis

PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to DIN 38407-42 for method-specific nomenclature.

AcronymCompound NameCAS#
PFBSPerfluoro-1-butanesulfonic acid375-73-5
PFDAPerfluorodecanoic acid335-76-2
PFHpAPerfluoroheptanoic acid375-85-9
PFHxSPerfluoro-1-hexanesulfonic acid355-46-4
PFHxAPerfluorohexanoic acid307-24-4
PFNAPerfluorononanoic acid375-95-1
PFOSPerfluorooctanesulfonic acid1763-23-1
PFOAPerfluorooctanoic acid335-67-1
PFBAPerfluorobutanoic acid375-22-4
PFPeAPerfluoropentanoic acid2706-90-3

Recommended Products for DIN 38407-42 for PFAS Analysis

Analytical LC Columns:


Raptor C18, 2.7 µm, 50 x 2.1 mm HPLC Column
Force C18, 1.8 µm, 50 x 2.1 mm LC Column

Delay Columns:


PFAS Delay Column, 5 µm, 50 x 2.1 mm HPLC Column

PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])

Guard Columns:


UltraShield UHPLC PreColumn Filter, 0.2 µm Frit, ea.
EXP Direct Connect Holder for EXP Guard Cartridges, Includes Fitting & Ferrules
Raptor C18 EXP Guard Column Cartridge, 2.7 µm, 5 x 2.1 mm, 3-pk.

Standards:


PFAS 24 Calibration Standard, 1 µg/mL, Methanol (1 mM KOH), 1 mL/ampul
PFAS 28 Calibration Standard, 1 µg/mL, Methanol (1 mM KOH), 1 mL/ampul
Discontinued: EPA 533 PFAS Calibration Standard, 2 µg/mL, Methanol (1 mM KOH), 1 mL/ampul

Sample Prep:


30 mm Syringe Filter, 0.45 µm, Nylon, Pink, 100-pk.
30 mm Syringe Filter, 0.22 µm, Nylon, Pink, 100-pk.
Resprep Polymeric SPE Cartridge, WAX, 6 mL/500 mg, 30 μm, 30-pk.
Resprep Polymeric SPE Cartridge, WAX, 6 mL/200 mg, 30 μm, 30-pk.
SPE Empty Tubes, Sample Reservoir, 75 mL, Polypropylene, 12-pk.
SPE Connector, 1, 3, 6, 10, or 15 mL, Polypropylene, 15-pk.

*Note that a regenerated cellulose filter is mentioned in the method. However, nylon and polypropylene are widely accepted filter membrane materials for PFAS analysis.

Vacuum Manifolds:


Resprep QR-24 Vacuum Manifold
Resprep QR-12 Vacuum Manifold

Vacuum Pumps:


Chemker 300 PTFE Vacuum Pump, 20 L/min, AC110 V, 60 Hz
Chemker 300 PTFE Vacuum Pump, 18 L/min, AC220 V, 50 Hz

General Lab Supplies:


Limited-Volume Screw-Thread Polypropylene Vials, 9 mm, 700 µL, 12 x 32 mm, 100-pk.
Limited-Volume Screw-Thread Polypropylene Vials, 9 mm, 700 µL, 12 x 32 mm, 1000-pk.
2.0 mL, 9 mm Solid-Top Polyethylene Caps, Screw-Thread, 10 mil thick membrane, Clear, 100-pk.
2.0 mL, 9 mm Solid-Top Polyethylene Caps, Screw-Thread, 10 mil thick membrane, Clear, 1000-pk.
Empty Centrifuge Tubes, 50 mL, Polypropylene w/Cap, 50-pk.
Empty Centrifuge Tubes, 50 mL, Polypropylene w/Cap, 500-pk.
Norm-Ject Plastic Syringe, 10 mL Luer Lock Tip, 100-pk.
Norm-Ject Plastic Syringe, 5 mL Luer Lock Tip, 100-pk.

Discover More Solutions for PFAS Analysis!

EZLC modeler PFAS Placeholder Image

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  • Restek Corporation

    Restek is a leading provider of chromatography columns, accessories, and certified reference materials. Trust Restek for reliable, high-quality analytical solutions.

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