- Sample Matrix: aqueous samples
- Sample Preparation: filtration
- Detection Technique: LC-MS/MS
Standard Test Method for Determination of Per- and Polyfluoroalkyl Substances (PFAS) in Aqueous Matrices by Co-solvation followed by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
ASTM D8421-22 is used for the analysis of 44 PFAS in aqueous sample matrices. It combines filtration sample preparation with LC-MS/MS analysis. ASTM D8421-22 is not available from Restek, but it may be purchased from ASTM. The full analyte list and products recommended by Restek are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.
Analyte List for ASTM D8421-22 for PFAS Analysis
PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to ASTM D8421-22 for method-specific nomenclature.
| Acronym | Compound Name | CAS# |
| NEtFOSAA | N-ethyl perfluorooctanesulfonamidoacetic acid | 2991-50-6 |
| NMeFOSAA | N-methyl perfluorooctanesulfonamidoacetic acid | 2355-31-9 |
| PFBS | Perfluoro-1-butanesulfonic acid | 375-73-5 |
| PFDA | Perfluorodecanoic acid | 335-76-2 |
| PFDoA | Perfluorododecanoic acid | 307-55-1 |
| PFHpA | Perfluoroheptanoic acid | 375-85-9 |
| PFHxS | Perfluoro-1-hexanesulfonic acid | 355-46-4 |
| PFHxA | Perfluorohexanoic acid | 307-24-4 |
| PFNA | Perfluorononanoic acid | 375-95-1 |
| PFOS | Perfluorooctanesulfonic acid | 1763-23-1 |
| PFOA | Perfluorooctanoic acid | 335-67-1 |
| PFTeDA | Perfluorotetradecanoic acid | 376-06-7 |
| PFTrDA | Perfluorotridecanoic acid | 72629-94-8 |
| PFUnA | Perfluoroundecanoic acid | 2058-94-8 |
| HFPO-DA, GenX | Hexafluoropropylene oxide dimer acid | 13252-13-6 |
| PFBA | Perfluorobutanoic acid | 375-22-4 |
| PFPeA | Perfluoropentanoic acid | 2706-90-3 |
| PFDS | Perfluoro-1-decanesulfonic acid | 335-77-3 |
| PFHpS | Perfluoro-1-heptanesulfonic acid | 375-92-8 |
| PFPeS | Perfluoro-1-pentanesulfonic acid | 2706-91-4 |
| FOSA | Perfluoro-1-octanesulfonamide | 754-91-6 |
| 4:2 FTS | 1H,1H,2H,2H-Perfluorohexane sulfonic acid | 757124-72-4 |
| 6:2 FTS | 1H,1H,2H,2H -Perfluorooctane sulfonic acid | 27619-97-2 |
| 8:2 FTS | 1H,1H,2H,2H -Perfluorodecane sulfonic acid | 39108-34-4 |
| 8:2 FTUCA, FOUEA | 2H-perfluoro-2-decenoic acid | 70887-84-2 |
| NMeFOSA | N-Methylperfluorooctanesulfonamide | 31506-32-8 |
| NEtFOSA | N-ethylperfluorooctanesulfonamide | 4151-50-2 |
| 9Cl-PF3ONS | 9-Chlorohexadecafluoro-3-oxanonane-1-sulfonic acid (F-53B Major) | 756426-58-1 |
| 11Cl-PF3OUdS | 11-Chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (F-53B Minor) | 763051-92-9 |
| ADONA | 4,8-Dioxa-3H-perfluorononanoic acid | 919005-14-4 |
| PFNS | Perfluoro-1-nonanesulfonic acid | 68259-12-1 |
| 6:2 FHUEA | 2H-perfluoro-2-octenoic acid | 70887-88-6 |
| PFEESA | Perfluoro(2-ethoxyethane)sulfonic acid | 113507-82-7 |
| NFDHA | Nonafluoro-3,6-dioxaheptanoic acid | 151772-58-6 |
| PFMPA | Perfluoro-3-methoxypropanoic acid | 377-73-1 |
| PFMBA | Perfluoro-4-methoxybutanoic acid | 863090-89-5 |
| NMeFOSE | 2-(N-methylperfluoro-1-octanesulfonamido)-ethanol | 24448-09-07 |
| NEtFOSE | 2-(N-ethylperfluoro-1-octanesulfonamido)-ethanol | 1691-99-2 |
| PFDoS | Perfluorododecane sulfonate | 79780-39-5 |
| 3:3 FTCA | 3:3 Fluorotelomer carboxylic acid | 356-02-5 |
| 5:3 FTCA | 5:3 Fluorotelomer carboxylic acid | 914637-49-3 |
| 7:3 FTCA, FHpPA | 7:3 Fluorotelomer carboxylic acid or 3-perfluoroheptyl propanoic acid | 812-70-4 |
| LiTFSI (LiTFSA, HQ-115) | Lithium Bis(trifluoromethane)sulfonimide | 90076-65-6 |
| PFPrA | Pentafluoropropanoic acid | 422-64-0 |
Recommended Products for ASTM D8421-22 for PFAS Analysis
Analytical LC Columns:
PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])
Guard Columns:
Sample Prep:
General Lab Supplies:
* Note that ASTM methods refer to the use of a glass syringe. Norm-Ject plastic syringes are made from polypropylene/polyethylene and can be used as an alternative.
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