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PFAS Analysis by U.S. USDA CLG-PFAS 2.04 for Bovine, Porcine, Poultry, Siluriformes Muscle, and Bovine Plasma

03 Oct 2025

feature FSAR4290
  • Sample Matrix: bovine, porcine, poultry, and Siluriformes muscle and bovine plasma
  • Sample Preparation: protein precipitation extraction
  • Detection Technique: UHPLC-MS/MS

Screening, Determination, and Confirmation of PFAS by UHPLC-MS-MS

USDA Method CLG-PFAS 2.04 is used for the analysis of 16 PFAS in bovine, porcine, poultry, Siluriformes muscle samples, and in bovine plasma samples. It combines a methanol-based protein precipitation extraction with UHPLC-MS/MS analysis on a C18 column. The method is performance based and allows equivalent equipment, reagents, or solutions to be used, and some instrument parameters can be optimized as detailed in the method to improve chromatography and detection. The full analyte list and recommended products for PFAS analysis in bovine, porcine, poultry, Siluriformes muscle, and bovine plasma samples by CLG-PFAS 2.04 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.

Analyte List for USDA CLG-PFAS 2.04 for PFAS Analysis

PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to U.S. USDA CLG-PFAS 2.04 for method-specific nomenclature.

AcronymCompound NameCAS#
PFBSPerfluoro-1-butanesulfonic acid375-73-5
PFDAPerfluorodecanoic acid335-76-2
PFDoAPerfluorododecanoic acid307-55-1
PFHpAPerfluoroheptanoic acid375-85-9
PFHxSPerfluoro-1-hexanesulfonic acid355-46-4
PFHxAPerfluorohexanoic acid307-24-4
PFNAPerfluorononanoic acid375-95-1
PFOSPerfluorooctanesulfonic acid1763-23-1
PFOAPerfluorooctanoic acid335-67-1
PFTeDAPerfluorotetradecanoic acid376-06-7
PFTrDAPerfluorotridecanoic acid72629-94-8
PFUnAPerfluoroundecanoic acid2058-94-8
PFPeAPerfluoropentanoic acid2706-90-3
PFDSPerfluoro-1-decanesulfonic acid335-77-3
PFHxDAPerfluoro-n-hexadecanoic acid67905-19-5
PFODAPerfluoro-n-octadecanoic acid16517-11-6

Recommended Products for U.S. USDA CLG-PFAS for PFAS Analysis

Analytical LC Columns:


Raptor C18, 2.7 µm, 50 x 2.1 mm HPLC Column
Force C18, 1.8 µm, 50 x 2.1 mm LC Column

Delay Columns:


PFAS Delay Column, 5 µm, 50 x 2.1 mm HPLC Column

PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])

Guard Columns:


UltraShield UHPLC PreColumn Filter, 0.2 µm Frit, ea.
EXP Direct Connect Holder for EXP Guard Cartridges, Includes Fitting & Ferrules
Raptor C18 EXP Guard Column Cartridge, 2.7 µm, 5 x 2.1 mm, 3-pk.

Standards:


EPA 537.1 PFAS Calibration Standard, 2 µg/mL, Methanol (1 mM KOH), 1 mL/ampul
Discontinued: EPA 533 PFAS Calibration Standard, 2 µg/mL, Methanol (1 mM KOH), 1 mL/ampul

Sample Prep:


Q-sep Multispeed Centrifuge for QuEChERS

Sample Reservoirs:


SPE Empty Tubes, Sample Reservoir, 75 mL, Polypropylene, 12-pk.
SPE Connector, 1, 3, 6, 10, or 15 mL, Polypropylene, 15-pk.

Vacuum Manifolds:


Resprep QR-24 Vacuum Manifold
Resprep QR-12 Vacuum Manifold

Vacuum Pumps:


Chemker 300 PTFE Vacuum Pump, 20 L/min, AC110 V, 60 Hz
Chemker 300 PTFE Vacuum Pump, 18 L/min, AC220 V, 50 Hz

General Lab Supplies:


Limited-Volume Screw-Thread Polypropylene Vials, 9 mm, 700 µL, 12 x 32 mm, 100-pk.
Limited-Volume Screw-Thread Polypropylene Vials, 9 mm, 700 µL, 12 x 32 mm, 1000-pk.
2.0 mL, 9 mm Solid-Top Polyethylene Caps, Screw-Thread, 10 mil thick membrane, Clear, 100-pk.
2.0 mL, 9 mm Solid-Top Polyethylene Caps, Screw-Thread, 10 mil thick membrane, Clear, 1000-pk.
Empty Centrifuge Tubes, 50 mL, Polypropylene w/Cap, 50-pk.
Empty Centrifuge Tubes, 50 mL, Polypropylene w/Cap, 500-pk.
Norm-Ject Plastic Syringe, 10 mL Luer Lock Tip, 100-pk.
Norm-Ject Plastic Syringe, 5 mL Luer Lock Tip, 100-pk.

Discover More Solutions for PFAS Analysis!

EZLC modeler PFAS Placeholder Image

Author

  • Restek Corporation

    Restek is a leading provider of chromatography columns, accessories, and certified reference materials. Trust Restek for reliable, high-quality analytical solutions.

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