The plot has lines that represent hypothetical retention times for compounds that elute at different points in the gradient profile. They are not representations of your actual chromatography. In addition, the time you enter as the end of the gradient (7 minutes in this example) is the time at which the pumps switch over to the final %B. Extra time is required for that final mobile phase concentration to travel through the system to the detector.
The gradient for my translated method goes to 7 minutes, but I’m seeing a retention time marker appear at 8.3 minutes in the translation validation plot (Windows version only). Why is it coming out after my run is complete?
30 Jul 2025
Author
GNOT5137
