{"id":103505,"date":"2020-10-28T14:30:00","date_gmt":"2020-10-28T14:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/?p=103505"},"modified":"2026-09-16T08:55:46","modified_gmt":"2026-09-16T08:55:46","slug":"lc-column-particle-choice-fpp-spp","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/zh-hans\/articles-zh\/lc-column-particle-choice-fpp-spp\/","title":{"rendered":"\u901a\u7528\u5e94\u7528\u65b9\u6848\u9897\u7c92\u9009\u62e9\u5bf9\u8272\u8c31\u67f1\u6027\u80fd\u7684\u5f71\u54cd\uff1a\u4ece3 \u03bcm\u548c5 \u03bcm\u5168\u591a\u5b54\u9897\u7c92(FPP)\u5207\u6362\u81f35 \u03bcm\u8868\u9762\u591a\u5b54\u9897\u7c92(SPP)"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">\u5f15\u8a00<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u8868\u9762\u591a\u5b54\u9897\u7c92\uff08\u901a\u5e38\u79f0\u4e3aSPP\u6216\u201c\u6838-\u58f3\u201d\u9897\u7c92\uff09\u5df2\u88ab\u8bc1\u660e\u53ef\u5b9e\u73b0\u5feb\u901f\u3001\u9ad8\u6548\u7684\u6db2\u76f8\u5206\u79bb\u3002\u8fd9\u79cd\u9897\u7c92\u5177\u6709\u4e00\u4e2a\u5b9e\u5fc3\u7684\u3001\u4e0d\u53ef\u6e17\u900f\u7684\u6838\u5fc3\uff0c\u6838\u5fc3\u5916\u90e8\u5305\u8986\u7740\u4e00\u5c42\u8584\u8584\u7684\u591a\u5b54\u7845\u80f6\u58f3\u5c42\uff0c\u4e0e\u4f20\u7edf\u7684\u5168\u591a\u5b54\u9897\u7c92\uff08FPP\uff09\u76f8\u6bd4\uff0c\u53ef\u663e\u8457\u63d0\u9ad8\u5206\u6790\u7684\u6548\u7387\u548c\u7075\u654f\u5ea6\u3002\u5728\u672c\u6280\u672f\u8bf4\u660e\u4e2d\uff0c\u6211\u4eec\u5c06\u6bd4\u8f83Raptor 5 \u03bcm \u6838\u58f3\u6db2\u76f8\u67f1\u4e0e\u4f20\u7edf3 \u03bcm\u548c5 \u03bcm \u5168\u591a\u5b54\u6db2\u76f8\u67f1\u7684\u6027\u80fd\u3002\u672c\u6587\u5c55\u793a\u4e86\uff0c\u65e0\u9700\u66f4\u6362\u4eea\u5668\u8bbe\u5907\uff0c\u53ea\u901a\u8fc7\u5c06\u5168\u591a\u5b54\u8272\u8c31\u67f1\u66f4\u6362\u4e3a\u6838\u58f3\u67f1\uff0c\u5373\u53ef\u5b9e\u73b0\u66f4\u5feb\u3001\u66f4\u9ad8\u6548\u7684\u5206\u6790\uff0c\u540c\u65f6\u83b7\u5f97\u66f4\u9ad8\u7684\u7075\u654f\u5ea6\u548c\u66f4\u4f4e\u7684\u7cfb\u7edf\u538b\u529b\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u4ece FPP \u5207\u6362\u81f3 SPP\uff1a\u66f4\u9ad8\u67f1\u6548\uff0c\u66f4\u4f4e\u538b\u529b<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">SPP \u7684\u4e3b\u8981\u4f18\u52bf\u4e4b\u4e00\u5728\u4e8e\uff0c\u4e0e\u5e38\u89c4 FPP \u76f8\u6bd4\uff0c\u5b83\u80fd\u591f\u5728\u63d0\u4f9b\u66f4\u9ad8\u67f1\u6548\u7684\u540c\u65f6\uff0c\u5f80\u5f80\u8fd8\u80fd\u4fdd\u6301\u76f8\u8fd1\u751a\u81f3\u66f4\u4f4e\u7684\u80cc\u538b\u3002\u901a\u8fc7\u63d0\u9ad8\u67f1\u6548\u540c\u65f6\u964d\u4f4e\u538b\u529b\uff0c\u7528\u6237\u65e0\u9700\u66f4\u6362\u4eea\u5668\u5373\u53ef\u7f29\u77ed\u5206\u6790\u65f6\u95f4\u3002\u5982\u56fe1\u6240\u793a\uff0c\u5f53\u4ece3 \u03bcmFPP\u8272\u8c31\u67f1\u5207\u6362\u81f3Raptor\u2122 5 \u03bcm SPP\u8272\u8c31\u67f1\u65f6\uff0c\u5728\u6240\u6d4b\u8bd5\u7684\u4eea\u5668\u6d41\u901f\u8303\u56f4\uff080.2\u81f32mL\/min\uff09\u5185\uff0c\u8272\u8c31\u67f1\u80cc\u538b\u5e73\u5747\u964d\u4f4e\u7ea650%\u3002\u867d\u71365 \u03bcm FPP\u8272\u8c31\u67f1\u7684\u538b\u529b\u4e0eRaptor\u2122 5\u03bcm SPP\u8272\u8c31\u67f1\u76f8\u5f53\uff0c\u4f46\u8fd9\u662f\u4ee5\u727a\u7272\u67f1\u6548\u4e3a\u4ee3\u4ef7\u7684\u3002<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\">\u56fe 1\uff1a\u4ece 3 \u03bcm FPP \u8272\u8c31\u67f1\u5207\u6362\u81f3 Raptor 5 \u03bcm SPP \u8272\u8c31\u67f1\uff0c\u80cc\u538b\u964d\u4f4e\u4e00\u534a<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2109-01.jpg\" alt=\"chart, line chart\" title=\"-\"><\/figure>\n\n<\/div><\/div><\/div>\n\n\n<p class=\"wp-block-paragraph\">\u5355\u4f4d\u538b\u529b\u4e0b\u7684\u7406\u8bba\u5854\u677f\u6570\uff08N\uff09\u6bd4\u503c\u53ef\u4f5c\u4e3a\u8861\u91cf\u8272\u8c31\u67f1\u67f1\u6548\u7684\u4e00\u4e2a\u6307\u6807\u3002\u5bf9\u6bcf\u6839\u8272\u8c31\u67f1\u8fdb\u884c\u6bd4\u8f83\u65f6\uff0cRaptor\u2122 5 \u03bcm SPP \u8272\u8c31\u67f1\u7684\u5355\u4f4d\u538b\u529b\u5854\u677f\u6570\u662f 5 \u03bcm FPP \u8272\u8c31\u67f1\u7684\u4e24\u500d\uff0c\u662f 3 \u03bcm FPP \u8272\u8c31\u67f1\u7684\u56db\u500d\uff08\u56fe2\uff09<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u53e6\u5916\uff0c\u67f1\u6548\u4e0e\u538b\u529b\u4e4b\u95f4\u7684\u5173\u7cfb\u4e5f\u53ef\u4ee5\u901a\u8fc7\u4fdd\u6301\u538b\u529b\u76f8\u5bf9\u6052\u5b9a\u6765\u6bd4\u8f83\u5404\u8272\u8c31\u67f1\u7684\u7406\u8bba\u5854\u677f\u6570\uff08\u56fe3\uff09\u3002\u540c\u6837\uff0cRaptor 5 \u03bcmSPP\u8272\u8c31\u67f1\u5728\u76f8\u540c\u538b\u529b\u4e0b\u663e\u793a\u51fa\u67f1\u6548\u7684\u663e\u8457\u63d0\u5347\u3002<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\">\u56fe 2\uff1a\u4ece\u5355\u4f4d\u538b\u529b\u5854\u677f\u6570\u6765\u770b\uff0c\u4ece 3 \u03bcm \u548c 5 \u03bcm FPP \u8272\u8c31\u67f1\u5207\u6362\u81f3 Raptor 5 \u03bcm \u6838\u58f3\u67f1\u5177\u6709\u660e\u663e\u4f18\u52bf<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"589\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2109-02-1024x589.jpg\" alt=\"\" class=\"wp-image-20650\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2109-02-1024x589.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2109-02-300x173.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2109-02-768x442.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2109-02-1536x884.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2109-02.jpg 1800w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\">\u56fe 3\uff1a\u5728\u76f8\u540c\u538b\u529b\u4e0b\uff0cRaptor 5 \u03bcm \u6838\u58f3\u9897\u7c92\u8868\u73b0\u51fa\u6bd4 3 \u03bcm \u548c 5 \u03bcm \u5168\u591a\u5b54\u9897\u7c92\u663e\u8457\u66f4\u9ad8\u7684\u5854\u677f\u6570<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-GNAR2109-03.jpg\" alt=\"chart, bar chart, waterfall chart\" title=\"-\"><\/figure>\n\n<\/div><\/div><\/div>\n\n\n<p class=\"wp-block-paragraph\">Raptor 5 \u03bcm\u8272\u8c31\u67f1\u7684\u5854\u677f\u6570\u51e0\u4e4e\u662f\u5168\u591a\u5b543 \u03bcm\u548c5 \u03bcm\u9897\u7c92\u8272\u8c31\u67f1\u7684\u4e24\u500d\uff08\u5373\u67f1\u6548\u66f4\u9ad8\uff09\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">\u4ece FPP \u5207\u6362\u81f3 SPP\uff1a\u66f4\u9ad8\u67f1\u6548\uff0c\u66f4\u77ed\u8fd0\u884c\u65f6\u95f4<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">SPP\u7684\u51fa\u73b0\u4f7f\u5206\u6790\u4eba\u5458\u65e0\u9700\u6602\u8d35\u7684\u8d85\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\uff08UHPLC\uff09\u4eea\u5668\u5373\u53ef\u5b9e\u73b0\u5feb\u901f\u5206\u79bb\uff0c\u4ece\u800c\u65e0\u9700\u8d44\u672c\u6295\u5165\u5373\u53ef\u63d0\u9ad8\u6837\u54c1\u901a\u91cf\u3002\u4e3a\u8003\u5bdf\u8868\u9762\u591a\u5b54\u9897\u7c92\u5728\u901f\u5ea6\u548c\u67f1\u6548\u65b9\u9762\u7684\u63d0\u5347\uff0c\u6211\u4eec\u91c7\u7528\u76f8\u540c\u7684\u4eea\u5668\u548c\u7b49\u5ea6\u65b9\u6cd5\u6761\u4ef6\uff0c\u5c06Raptor Biphenyl 5\u03bcm SPP\u8272\u8c31\u67f1\u4e0e\u586b\u5145\u5168\u591a\u5b543 \u03bcm\u548c5 \u03bcm\u9897\u7c92\u7684\u8272\u8c31\u67f1\u8fdb\u884c\u4e86\u5bf9\u6bd4\u5206\u6790\uff08\u56fe4\uff09\u3002<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\">\u56fe 4\uff1a\u4e0e 3 \u03bcm FPP \u76f8\u6bd4\uff0c\u5207\u6362\u81f3 5 \u03bcm SPP \u53ef\u964d\u4f4e\u538b\u529b\uff0c\u5206\u6790\u65f6\u95f4\u51e0\u4e4e\u7f29\u77ed\u4e00\u534a\uff1b\u4e0e 5 \u03bcm FPP \u76f8\u6bd4\uff0c\u67f1\u6548\u663e\u8457\u66f4\u4f18<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0556.png\" alt=\"Comparison: Efficiency and Analysis Time for Raptor\u2122 Biphenyl 5 \u00b5m and Fully Porous 5 \u00b5m and 3 \u00b5m Columns\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0556<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">Conc.<br \/>(mg\/mL)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Uracil\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/66-22-8\/Uracil\" target=\"_blank\" rel=\"noopener\">Uracil<\/a><\/td><td class=\"oth\">0.02<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Benzene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/71-43-2\/Benzene\" target=\"_blank\" rel=\"noopener\">Benzene<\/a><\/td><td class=\"oth\">3.0<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Naphthalene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/91-20-3\/Naphthalene\" target=\"_blank\" rel=\"noopener\">Naphthalene<\/a><\/td><td class=\"oth\">0.5<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Biphenyl\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/92-52-4\/Biphenyl\" target=\"_blank\" rel=\"noopener\">Biphenyl<\/a><\/td><td class=\"oth\">0.06<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>LC Reversed Phase Test Mix #1 (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/35005\" rel=\"noopener\">cat.# 35005<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Methanol:water (75:25)<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.8<\/td><td>45<\/td><td>55<\/td><\/tr><tr><td>20<\/td><td>0.8<\/td><td>45<\/td><td>55<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>DAD @ 254 nm<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>All columns were 150 mm x 4.6 mm ID. Values for efficiency (N) were calculated from the Biphenyl peak.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-related-skus\"><h4>Related SKUs<\/h4><ul><li>35005<\/li><\/ul><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0556.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5728Raptor Biphenyl 5 \u03bcm\u8272\u8c31\u67f1\u4e0a\uff0c\u6700\u540e\u4e00\u4e2a\u6d17\u8131\u7684\u5206\u6790\u7269\u51fa\u5cf0\u65f6\u95f4\u4e3a7.1\u5206\u949f\uff0c\u4e0e\u5168\u591a\u5b543 \u03bcm\u548c5 \u03bcm\u9897\u7c92\u8272\u8c31\u67f1\uff08\u8fd0\u884c\u65f6\u95f4\u5206\u522b\u4e3a13.6\u5206\u949f\u548c12.9\u5206\u949f\uff09\u76f8\u6bd4\uff0c\u5206\u6790\u65f6\u95f4\u7f29\u77ed\u4e8645%\u3002\u6b64\u5916\uff0cRaptor Biphenyl 5 \u03bcm SPP\u8272\u8c31\u67f1\u7684\u67f1\u6548\u6bd45 \u03bcmFPP\u8272\u8c31\u67f1\u63d0\u9ad8\u4e8643%\u30023 \u03bcm FPP\u8272\u8c31\u67f1\u7684\u67f1\u6548\u4ec5\u7565\u4f4e\uff0c\u4f46\u5176\u80cc\u538b\u51e0\u4e4e\u662fRaptor Biphenyl 5 \u03bcm SPP\u8272\u8c31\u67f1\u7684\u4e24\u500d\u3002<\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">\u4ece FPP \u5207\u6362\u81f3 SPP\uff1a\u66f4\u9ad8\u7075\u654f\u5ea6\uff0c\u66f4\u4f18\u6027\u80fd<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u5c06\u5e38\u89c4\u76843 \u03bcm\u62165 \u03bcm FPP\u8272\u8c31\u67f1\u66ff\u6362\u4e3aRaptor 5 \u03bcm\u8272\u8c31\u67f1\uff0c\u53ef\u7acb\u7aff\u89c1\u5f71\u5730\u63d0\u5347\u73b0\u6709\u65b9\u6cd5\u7684\u6027\u80fd\u3002\u5982\u56fe5\u6240\u793a\uff0c\u5728\u68af\u5ea6\u6761\u4ef6\u4e0b\uff0c\u65b9\u6cd5\u7684\u67f1\u6548\u3001\u5cf0\u5bbd\u548c\u5206\u6790\u65f6\u95f4\u5747\u53ef\u5f97\u5230\u6539\u5584\u3002<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\">\u56fe 5\uff1a\u4ece FPP \u5207\u6362\u81f3 Raptor SPP \u8272\u8c31\u67f1\uff0c\u63d0\u9ad8\u5206\u79bb\u5ea6\u5e76\u7f29\u77ed\u5206\u6790\u65f6\u95f4<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0555.png\" alt=\"Comparison: Fentanyl, Norfentanyl, and Two Analogues on Raptor\u2122 Biphenyl 5 \u00b5m and Fully Porous 5 \u00b5m Columns\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_GN0555<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Norfentanyl\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1609-66-1\/Norfentanyl\" target=\"_blank\" rel=\"noopener\">Norfentanyl<\/a><\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Acetyl fentanyl\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/3258-84-2\/Acetyl fentanyl\" target=\"_blank\" rel=\"noopener\">Acetyl fentanyl<\/a><\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Fentanyl\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/437-38-7\/Fentanyl\" target=\"_blank\" rel=\"noopener\">Fentanyl<\/a><\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sufentanyl\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56030-54-7\/Sufentanyl\" target=\"_blank\" rel=\"noopener\">Sufentanyl<\/a><\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>25 \u00b5g\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water + 0.1% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol + 0.1% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.6<\/td><td>65<\/td><td>35<\/td><\/tr><tr><td>2.00<\/td><td>0.6<\/td><td>15<\/td><td>85<\/td><\/tr><tr><td>2.01<\/td><td>0.6<\/td><td>65<\/td><td>35<\/td><\/tr><tr><td>4.00<\/td><td>0.6<\/td><td>65<\/td><td>35<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>PDA @ 210 nm<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>All columns were 50 mm x 2.1 mm ID.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_gn0555.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5728Raptor Biphenyl 5 \u03bcm \u6838\u58f3\u67f1\u4e0a\uff0c\u5e73\u5747\u5206\u79bb\u5ea6\u503c\u4e3a6.0\uff0c\u800c\u57285 \u03bcm \u5168\u591a\u5b54\u8272\u8c31\u67f1\u4e0a4.1\u3002\u9664\u4e86\u66f4\u77ed\u7684\u8fd0\u884c\u65f6\u95f4\u5916\uff0c\u5207\u6362\u81f3\u76f8\u540c\u7c92\u5f84\u7684\u6838\u58f3\u67f1\u4e0a\u4ee5\u540e\uff0c\u5206\u6790\u7269\u7684\u5e73\u5747\u5206\u79bb\u5ea6\u63d0\u9ad8\u4e8646%\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5728\u53e6\u4e00\u4e2a\u4f7f\u7528\u68af\u5ea6\u6761\u4ef6\u548c\u8d28\u8c31\u68c0\u6d4b\u7684\u5b9e\u4f8b\u4e2d\uff0c\u5206\u6790\u65f6\u95f4\u4ece5 \u03bcm \u5168\u591a\u5b54\u8272\u8c31\u67f1\u76842.3min\u7f29\u77ed\u81f3Raptor Biphenyl 5 \u03bcm\u6838\u58f3\u67f1\u76841.5min\uff08\u56fe6\uff09\u3002\u540c\u65f6\uff0c5 \u03bcm \u6838\u58f3\u67f1\u7684\u5e73\u5747\u7075\u654f\u5ea6\u6bd43 \u03bcm \u5168\u591a\u5b54\u8272\u8c31\u67f1\u63d0\u9ad8\u4e8664%\uff0c\u6bd45 \u03bcm \u5168\u591a\u5b54\u8272\u8c31\u67f1\u63d0\u9ad8\u4e86128%\u3002<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\">\u56fe 6\uff1aRaptor 5 \u03bcm \u6838\u58f3\u67f1\u76f8\u6bd4 3 \u03bcm \u6216 5 \u03bcm \u5168\u591a\u5b54\u8272\u8c31\u67f1\u8fd8\u80fd\u63d0\u4f9b\u66f4\u9ad8\u7684\u7075\u654f\u5ea6<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0598.png\" alt=\"Comparison: Thyroid Hormones on Raptor\u2122 Biphenyl 5 \u00b5m and Fully Porous 5 \u00b5m and 3 \u00b5m Columns\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0598<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">3,3&#8242;,5-Triiodo-L-thyronine (T3)<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\">3,3&#8242;,5-Triiodo-L-thyronine (rT3)<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\">L-Thyroxine (T4)<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>40 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Water:methanol (35:65)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>1 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>0.1% Formic acid in water <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>0.1% Formic acid in methanol <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.4<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>2.00<\/td><td>0.4<\/td><td>20<\/td><td>80<\/td><\/tr><tr><td>2.01<\/td><td>0.4<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>3.50<\/td><td>0.4<\/td><td>35<\/td><td>65<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>HPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Notes<\/th><td>All columns were 100 mm x 2.1 mm ID.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0598.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">\u7ed3\u8bba<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u4e0e\u76f8\u540c\u89c4\u683c\u76843 \u03bcm\u548c5 \u03bcm\u5168\u591a\u5b54\u9897\u7c92\uff08FPP\uff09\u8272\u8c31\u67f1\u76f8\u6bd4\uff0c\u91c7\u7528\u8868\u9762\u591a\u5b54\u9897\u7c92\uff08SPP\uff09\u7684Raptor 5 \u03bcm \u8272\u8c31\u67f1\u5177\u6709\u66f4\u4f4e\u7684\u80cc\u538b\u548c\u66f4\u9ad8\u7684\u67f1\u6548\u3002\u5728\u65b0\u65b9\u6cd5\u5f00\u53d1\u4e2d\uff0c\u4f7f\u7528Raptor 5 \u03bcm \u6838\u58f3\u67f1\u65e0\u9700\u5347\u7ea7\u4eea\u5668\u5373\u53ef\u5b9e\u73b0\u7f29\u77ed\u8fd0\u884c\u65f6\u95f4\u548c\u66f4\u9ad8\u7684\u65b9\u6cd5\u6027\u80fd\u3002\u5f53\u66ff\u6362\u73b0\u6709\u65b9\u6cd5\u4e2d\u5e38\u89c43 \u03bcm\u548c5 \u03bcm \u5168\u591a\u5b54\u8272\u8c31\u67f1\u65f6\uff0c\u5207\u6362\u81f3Raptor 5 \u03bcm \u6838\u58f3\u8272\u8c31\u67f1\u6709\u671b\u5927\u5e45\u7f29\u77ed\u5206\u6790\u65f6\u95f4\uff0c\u540c\u65f6\u63d0\u9ad8\u67f1\u6548\u548c\u7075\u654f\u5ea6\u3002\u67d0\u4e9b\u5e94\u7528\u53ef\u80fd\u9700\u8981\u4e00\u5b9a\u7a0b\u5ea6\u7684\u65b9\u6cd5\u4f18\u5316\u624d\u80fd\u8fbe\u5230\u6700\u4f73\u6548\u679c\uff0c\u4f46Raptor 5\u03bcm \u8272\u8c31\u67f1\u4e0e\u5927\u591a\u6570\u5e94\u7528\u517c\u5bb9\uff0c\u65e0\u9700\u989d\u5916\u6210\u672c\u6216\u4eba\u529b\u5373\u53ef\u63d0\u5347\u6027\u80fd\u7684\u7406\u60f3\u9009\u62e9\u3002<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u5f15\u8a00 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