{"id":104049,"date":"2020-10-21T00:00:00","date_gmt":"2020-10-21T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/?p=104049"},"modified":"2026-09-20T09:54:32","modified_gmt":"2026-09-20T09:54:32","slug":"pfas-lc-column-anatomy-phase-dimensions-particle-type","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/zh-hans\/articles-zh\/pfas-lc-column-anatomy-phase-dimensions-particle-type\/","title":{"rendered":"PFAS \u5206\u6790\u8272\u8c31\u67f1\u7684\u9009\u62e9\uff1a\u56fa\u5b9a\u76f8\u7c7b\u578b\u3001\u89c4\u683c\u4e0e\u9897\u7c92\u7c7b\u578b"},"content":{"rendered":"\n

\u5982\u679c\u60a8\u6240\u5728\u5b9e\u9a8c\u5ba4\u5f00\u5c55\u5168\u6c1f\u70f7\u57fa\u548c\u591a\u6c1f\u70f7\u57fa\u7269\u8d28\uff08PFAS\uff09\u7684\u6d4b\u8bd5\u5de5\u4f5c\uff0c\u90a3\u60a8\u4e00\u5b9a\u77e5\u9053\uff0c\u968f\u7740\u6211\u4eec\u5bf9\u8fd9\u7c7b\u201c\u6c38\u4e45\u6027\u5316\u5b66\u7269\u8d28\u201d\u7684\u666e\u904d\u6027\u3001\u6301\u4e45\u6027\u548c\u6f5c\u5728\u5065\u5eb7\u98ce\u9669\u7684\u66f4\u591a\u4e86\u89e3\uff0c\u4eba\u4eec\u5bf9\u8fd9\u7c7b\u5316\u5408\u7269\u7684\u5174\u8da3\u6b63\u5728\u4e0d\u65ad\u589e\u957f\u3002\u968f\u4e4b\u5bf9\u5feb\u901f\u3001\u51c6\u786e\u548c\u7cbe\u786e\u5206\u6790\u8fd9\u7c7b\u5316\u5408\u7269\u7684\u9700\u6c42\u4e5f\u5728\u6269\u5927\u3002\u8fd9\u4e9b\u9700\u6c42\u6b63\u63a8\u52a8\u66f4\u597d\u5206\u6790\u65b9\u6cd5\u7684\u51fa\u73b0\uff0c\u800c\u6db2\u76f8\u8272\u8c31\u67f1\u7684\u9009\u62e9\u662f\u6784\u5efa\u65b9\u6cd5\u7684\u57fa\u7840\u3002\u968f\u7740\u8d8a\u6765\u8d8a\u591a\u7684PFAS\u5316\u5408\u7269\u88ab\u5217\u5165\u7ba1\u63a7\u6e05\u5355\uff0c\u7279\u522b\u662f\u5bf9\u8d85\u77ed\u94fePFAS\u5316\u5408\u7269\u7684\u5173\u6ce8\uff0c\u65b0\u65b9\u6cd5\u7684\u5efa\u7acb\u53d8\u5f97\u5c24\u4e3a\u91cd\u8981\u3002\u5728\u8fd9\u91cc\uff0c\u6211\u4eec\u5c06\u4e0e\u5927\u5bb6\u63a2\u8ba8\u5982\u4f55\u6839\u636ePFAS\u76ee\u6807\u5f85\u6d4b\u53bb\u9009\u62e9\u5408\u9002\u7684\u6db2\u76f8\u8272\u8c31\u67f1\u3002<\/p>\n\n\n\n

\u56fa\u5b9a\u76f8\u7c7b\u578b\u548c\u89c4\u683c\u7684\u9009\u62e9<\/h2>\n\n\n\n

\u786e\u5b9a\u60a8\u5c06\u7814\u7a76\u7684PFAS\u5316\u5408\u7269\u8303\u56f4\uff0c\u5bf9\u4e8e\u8272\u8c31\u67f1\u56fa\u5b9a\u76f8\u7684\u9009\u62e9\u6700\u4e3a\u91cd\u8981\u3002\u9009\u62e9\u5bf9\u76ee\u6807\u5f85\u6d4b\u7269\u6709\u826f\u597d\u4fdd\u7559\u7684\u56fa\u5b9a\u76f8\u662f\u65b9\u6cd5\u5efa\u7acb\u7684\u7b2c\u4e00\u6b65\u3002<\/p>\n\n\n\n

\u77ed\u94fe\u53ca\u4ee5\u4e0a PFAS<\/h3>\n\n\n\n

\u5f53\u6d89\u53ca\u5230\u77ed\u94fePFAS\uff08C4-C6\uff09\u53ca\u4ee5\u4e0a\u7269\u8d28\u65f6\uff0c\u6211\u4eec\u5bf9\u4e0d\u540c\u7c7b\u578b\u8272\u8c31\u67f1\u5bf9\u6bd4\u540e\u53d1\u73b0\uff0cC18\u662f\u6700\u4f73\u9009\u62e9\u3002\u968f\u7740PFAS\u5206\u5b50\u4e0a\u7684\u70f7\u57fa\u94fe\u53d8\u957f\uff0c\u8fd9\u4e9b\u94fe\u4e0eC18\u914d\u4f53\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u589e\u52a0\uff0c\u4e3a\u4fdd\u7559\u548c\u5206\u79bb\u63d0\u4f9b\u4e86\u5f88\u597d\u7684\u673a\u5236\u3002\u56e0\u4e3a\u4fdd\u7559\u80fd\u529b\u8db3\u591f\u5f3a\uff0c\u56e0\u6b64\u53ef\u4ee5\u4f7f\u7528\u76f8\u5bf9\u8f83\u77ed\u7684\u7a84\u5185\u5f84\u8272\u8c31\u67f1\u5feb\u901f\u6709\u6548\u5730\u5206\u79bb\u76ee\u6807\u5316\u5408\u7269\u3002\u56fe1\u5c55\u793a\u7684\u662f\u7528RaptorC18\uff0c2.7 \u03bcm\uff0c50×2.1mm\u8272\u8c31\u67f1\u5728\u4e0d\u52308\u5206\u949f\uff08\u603b\u5206\u6790\u65f6\u95f4\u4e3a10\u5206\u949f\uff09\u65f6\u95f4\u5185\u8f7b\u677e\u5206\u79bb\u76ee\u6807\u5316\u5408\u7269\uff0c\u800c\u4e14\u6ee1\u8db3EPA537.1\u996e\u7528\u6c34\u6d4b\u8bd5\u6807\u51c6\u7684\u8981\u6c42\u3002<\/p>\n\n\n

\u56fe 1: Raptor C18\uff0850x 2.1mm \uff09\u8272\u8c31\u67f1\uff0c\u6839\u636e EPA 537.1 \u5206\u6790\u996e\u7528\u6c34\u4e2d PFAS\uff0c\u603b\u8fd0\u884c\u65f6\u95f4 10min<\/div>
\n
\"EPA<\/div>

LC_EV0560<\/p>

Peaks<\/h4>\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Conc.
(ng\/mL)<\/th>		Precursor Ion<\/th>Product Ion<\/th><\/tr><\/thead>\n
1.<\/td>Perfluorobutanesulfonic acid (PFBS)<\/a><\/td>2.06<\/td>10<\/td>298.8<\/td>79.9<\/td><\/tr>\n
2.<\/td>Perfluoro-n-[1,2-13<\/sup>C2<\/sub>]hexanoic acid (13<\/sup>C2<\/sub>-PFHxA)<\/td>3.03<\/td>5<\/td>314.9<\/td>270.0<\/td><\/tr>\n
3.<\/td>Perfluorohexanoic acid (PFHxA)<\/a><\/td>3.04<\/td>5<\/td>312.9<\/td>268.7<\/td><\/tr>\n
4.<\/td>Tetrafluoro-2-heptafluoropropoxy-13<\/sup>C3<\/sub>-propanoic acid (13<\/sup>C3<\/sub>-HFPO-DA)<\/td>3.36<\/td>5<\/td>286.8<\/td>168.7<\/td><\/tr>\n
5.<\/td>Hexafluoropropylene oxide dimer acid (HFPO-DA)<\/a><\/td>3.38<\/td>5<\/td>285.0<\/td>168.9<\/td><\/tr>\n
6.<\/td>Perfluoroheptanoic acid (PFHpA)<\/a><\/td>4.09<\/td>5<\/td>362.8<\/td>318.8<\/td><\/tr>\n
7.<\/td>Perfluorohexanesulfonic acid (PFHxS)<\/a><\/td>4.22<\/td>10<\/td>398.8<\/td>79.9<\/td><\/tr>\n
8.<\/td>4,8-Dioxa-3H-perfluorononanoic acid (ADONA)<\/a><\/td>4.24<\/td>5<\/td>376.9<\/td>250.7<\/td><\/tr>\n
9.<\/td>Perfluoro-[1,2-13<\/sup>C2<\/sub>]octanoic acid (13<\/sup>C2<\/sub>-PFOA)<\/td>4.88<\/td>5<\/td>415.0<\/td>370.0<\/td><\/tr>\n
10.<\/td>Perfluorooctanoic acid (PFOA)<\/a><\/td>4.90<\/td>5<\/td>413.1<\/td>368.9<\/td><\/tr>\n
11.<\/td>Perfluorononanoic acid (PFNA)<\/a><\/td>5.54<\/td>5<\/td>462.9<\/td>418.9<\/td><\/tr>\n
12.<\/td>Perfluoro-1-[1,2,3,4-13<\/sup>C4<\/sub>]octanesulfonic acid (13<\/sup>C4<\/sub>-PFOS)<\/td>5.57<\/td>10<\/td>503.0<\/td>80.0<\/td><\/tr>\n<\/tbody><\/table>\n\n
<\/th>Peaks<\/th>tR<\/sub> (min)<\/th>Conc.
(ng\/mL)<\/th>		Precursor Ion<\/th>Product Ion<\/th><\/tr><\/thead>
13.<\/td>Perfluorooctanesulfonic acid (PFOS)<\/a><\/td>5.58<\/td>10<\/td>498.9<\/td>80.0<\/td><\/tr>\n
14.<\/td>9-Chlorohexadecafluoro-3-oxanone-1-sulfonic acid (9Cl-PF3ONS)<\/td>5.88<\/td>5<\/td>530.8<\/td>350.7<\/td><\/tr>\n
15.<\/td>Perfluoro-n-[1,2-13<\/sup>C2<\/sub>]decanoic acid (13<\/sup>C2<\/sub>-PFDA)<\/td>6.08<\/td>5<\/td>514.9<\/td>469.9<\/td><\/tr>\n
16.<\/td>Perfluorodecanoic acid (PFDA)<\/a><\/td>6.08<\/td>5<\/td>512.9<\/td>469.0<\/td><\/tr>\n
17.<\/td>N-deuteriomethylperfluoro-1-octanesulfonamidoacetic acid (d3-NMeFOSAA)<\/td>6.28<\/td>10<\/td>572.9<\/td>418.8<\/td><\/tr>\n
18.<\/td>N-methyl perfluorooctanesulfonamidoacetic acid (NMeFOSAA)<\/a><\/td>6.30<\/td>10<\/td>569.8<\/td>418.8<\/td><\/tr>\n
19.<\/td>N-deuterioethylperfluoro-1-octanesulfonamidoacetic acid (d5-NEtFOSAA)<\/td>6.51<\/td>10<\/td>588.9<\/td>418.8<\/td><\/tr>\n
20.<\/td>N-ethyl perfluorooctanesulfonamidoacetic acid (NEtFOSAA)<\/a><\/td>6.52<\/td>10<\/td>583.8<\/td>418.8<\/td><\/tr>\n
21.<\/td>Perfluoroundecanoic acid (PFUnA)<\/a><\/td>6.55<\/td>5<\/td>562.9<\/td>518.8<\/td><\/tr>\n
22.<\/td>11-chloroeicosafluoro-3-oxaundecane-1-sulfonic acid (11Cl-PF3OUdS)<\/a><\/td>6.77<\/td>5<\/td>630.7<\/td>451.0<\/td><\/tr>\n
23.<\/td>Perfluorododecanoic acid (PFDoA)<\/a><\/td>6.95<\/td>5<\/td>612.7<\/td>568.9<\/td><\/tr>\n
24.<\/td>Perfluorotridecanoic acid (PFTrDA)<\/a><\/td>7.30<\/td>5<\/td>662.7<\/td>618.8<\/td><\/tr>\n
25.<\/td>Perfluorotetradecanoic acid (PFTA)<\/a><\/td>7.60<\/td>5<\/td>712.7<\/td>668.7<\/td><\/tr>\n<\/tbody><\/table><\/div>

Conditions<\/h4>
Column<\/th>Raptor C18 (cat.# 9304A52<\/a>)<\/td><\/tr>
Dimensions:<\/th>50 mm x 2.1 mm ID<\/td><\/tr>
Particle Size:<\/th>2.7 \u00b5m<\/td><\/tr>
Pore Size:<\/th>90 \u00c5<\/td><\/tr>
<\/td>
Temp.:<\/th>40 \u00b0C<\/td><\/tr>
Standard\/Sample<\/th><\/td><\/tr>
Diluent:<\/th>96:4 Methanol:water<\/td><\/tr>
Conc.:<\/th>5-10 ng\/mL<\/td><\/tr><\/td><\/tr>
Inj. Vol.:<\/th>2 \u00b5L <\/td><\/tr>
Mobile Phase<\/th><\/td><\/tr>
A:<\/th>Water, 5 mM ammonium acetate <\/td><\/tr>
B:<\/th>Methanol <\/td><\/tr>
<\/td>
Time (min)<\/th>Flow (mL\/min)<\/th>%A<\/th>%B<\/th><\/tr><\/thead>
0.00<\/td>0.4<\/td>70<\/td>30<\/td><\/tr>
8.00<\/td>0.4<\/td>10<\/td>90<\/td><\/tr>
8.01<\/td>0.4<\/td>70<\/td>30<\/td><\/tr>
10.0<\/td>0.4<\/td>70<\/td>30<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table>
Detector<\/th>MS\/MS<\/td><\/tr>
Ion Mode:<\/th>ESI- <\/td><\/tr>
Mode:<\/th>MRM <\/td><\/tr>
Instrument<\/th>HPLC<\/td><\/tr>
Notes<\/th>Want even better performance when analyzing metal-sensitive compounds? Check out Inert LC columns at www.restek.com\/inert<\/a>.<\/td><\/tr><\/table><\/div><\/div><\/div>

Related SKUs<\/h4>