In this scenario, the sample is initially concentrated onto the head of the column and moves through the column in a tight band. This technique, referred to as \u201con-column compression\u201d or \u201cpoint-injected,\u201d is sometimes used to minimize band broadening for a larger volume sample injection. Keep in mind that your sample components must be soluble in mobile phase as well in order for this to work.<\/p>\n","protected":false},"excerpt":{"rendered":"
In this scenario, the sample is initially concentrated onto the head of the column and moves through the column in a tight band. This technique, referred to as \u201con-column compression\u201d or \u201cpoint-injected,\u201d is sometimes used to minimize band broadening for a larger volume sample injection. Keep in mind that your sample components must be soluble…<\/p>\n","protected":false},"author":11,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[23],"tags":[2698,2682],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"hf_cat_post":[625],"ppma_author":[414],"class_list":["post-3265","post","type-post","status-publish","format-standard","hentry","category-faq","tag-faq-general-hplc","tag-faq-hplc-and-uhplc"],"acf":[],"taxonomy_info":{"category":[{"value":23,"label":"FAQ"}],"post_tag":[{"value":2698,"label":"FAQ: General HPLC"},{"value":2682,"label":"FAQ: HPLC and UHPLC"}]},"featured_image_src_large":false,"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/zh-hans\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":23,"name":"FAQ","slug":"faq","term_group":0,"term_taxonomy_id":23,"taxonomy":"category","description":"","parent":0,"count":271,"filter":"raw","cat_ID":23,"category_count":271,"category_description":"","cat_name":"FAQ","category_nicename":"faq","category_parent":0}],"tag_info":[{"term_id":2698,"name":"FAQ: General HPLC","slug":"faq-general-hplc","term_group":0,"term_taxonomy_id":2698,"taxonomy":"post_tag","description":"Browse general frequently asked questions for HPLC.","parent":0,"count":8,"filter":"raw"},{"term_id":2682,"name":"FAQ: HPLC and UHPLC","slug":"faq-hplc-and-uhplc","term_group":0,"term_taxonomy_id":2682,"taxonomy":"post_tag","description":"Browse frequently asked questions for HPLC and UHPLC products.","parent":0,"count":61,"filter":"raw"}],"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts\/3265","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/comments?post=3265"}],"version-history":[{"count":3,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts\/3265\/revisions"}],"predecessor-version":[{"id":70076,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts\/3265\/revisions\/70076"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=3265"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/categories?post=3265"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/tags?post=3265"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/industries-application?post=3265"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/post-badge?post=3265"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/resource-type?post=3265"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/product-library?post=3265"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/resource-technique?post=3265"},{"taxonomy":"hf_cat_post","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/hf_cat_post?post=3265"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/ppma_author?post=3265"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}