{"id":42485,"date":"2018-04-24T04:00:00","date_gmt":"2018-04-24T04:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/lc-column-storage\/"},"modified":"2025-11-26T18:57:58","modified_gmt":"2025-11-26T18:57:58","slug":"lc-column-storage","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/zh-hans\/videos-fr\/gnav3618\/lc-column-storage","title":{"rendered":"LC Column Storage"},"content":{"rendered":"\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"LC Column Storage\" width=\"720\" height=\"405\" src=\"https:\/\/www.youtube.com\/embed\/IacnT4Vt40k?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\">Description<\/h2>\n\n\n\n<p>Good chromatography isn\u2019t just about when the pumps are&nbsp;running. Taking care of your column when not in use&nbsp;is&nbsp;important for lifetime and reproducible performance.&nbsp;When shelving a column, be sure to flush out any buffers or other additives. You can use a solvent mix similar to initial run conditions, just without additives, and that can be good for a couple days of storage. For longer term storage, you should choose a storage solvent appropriate to your column and method. And always make sure to document the date and storage solvent, so you have the information you need to get back up and running as soon as possible.&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Additional Resources<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/articles\/restek-liquid-chromatography-lc-columns-cleaning-recommendations\" target=\"_blank\" rel=\"noopener\">Restek Liquid Chromatography Columns \u2013 Cleaning and Storage Recommendations<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/articles\/restek-liquid-chromatography-LC-columns-usage-care\/\" target=\"_blank\" rel=\"noopener\">Liquid Chromatography Columns \u2014 Usage &amp; Care<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.restek.com\/chromablography\/live-long-and-prosper-how-to-make-your-hplc-columns-last-longer\/\" target=\"_blank\" rel=\"noopener\">How to make your LC columns last longer<\/a><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">Restek Recommended Tools &amp; Parts<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.restek.com\/c\/1275\" target=\"_blank\" rel=\"noopener\">LC Columns<\/a><\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Transcript<\/h2>\n\n\n\n<p>Hi, everyone. Thanks for joining us for this Restek Tip. Our topic today is LC column storage. Now, you probably use a variety of phases in your lab&nbsp;because you know a C18 can\u2019t do everything. But when it comes time to give a column a break, it\u2019s important to store it properly, so it\u2019s ready to go the next time you need it. The first thing to do is flush out any buffers, acids, or bases that are part of mobile phase system. And,&nbsp;this is important because any particulate&nbsp;from&nbsp;the buffer could precipitate and clog the column. Too much acid left in the column could hydrolyze the ligand from the silica surface, and too much base could start to dissolve the silica&nbsp;particle itself. So, the best way to do this flushing is to prepare a mobile phase that matches the initial conditions of your run, but without the additives. Flush this through the column for 10 to 20 column volumes. And for short-term storage, like a couple of days, it\u2019s okay to leave this solvent in the column.<\/p>\n\n\n\n<p>If you\u2019re storing for a longer time, like a week or more, you flush initially as mentioned before, and then you prepare a storage solvent, with 50% water and 50% organic matching the solvent that you used in your method. Flush for another 10 to 20 column volumes. And for either type of storage, make sure the column plugs are tight so it doesn\u2019t dry out. It\u2019s a good idea to label the column with a date, and also the storage solvent, so you know how to properly equilibrate it the next time you need to put it back to work. Store the columns in a rack or in a drawer, and make sure to avoid temperature extremes to ensure long lifetime and good column performance. Thanks for joining us, and stay tuned for more tips from your friends at Restek.<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u201cDon\u2019t you forget about me!\u201d Listen to your LC columns, and give them a little TLC before you put them in storage. In this Restek Tip we talk about how to safely store your LC columns.<\/p>\n","protected":false},"author":11,"featured_media":41154,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[771],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[2391,2373],"resource-technique":[2299],"ppma_author":[414],"class_list":["post-42485","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-videos-fr","product-library-lc-columns","product-library-liquid-chromatography-products","resource-technique-liquid-chromatography"],"acf":[],"taxonomy_info":{"category":[{"value":771,"label":"Vid\u00e9os"}],"product-library":[{"value":2391,"label":"LC Columns"},{"value":2373,"label":"Liquid Chromatography Products"}],"resource-technique":[{"value":2299,"label":"Liquid Chromatography"}]},"featured_image_src_large":["https:\/\/discover.restek.com\/wp-content\/uploads\/feature-GNAV3618-1024x536.jpg",1024,536,true],"author_info":{"display_name":"Restek Corporation","author_link":"https:\/\/discover.restek.com\/zh-hans\/author\/restek-corporation\/"},"comment_info":0,"category_info":[{"term_id":771,"name":"Vid\u00e9os","slug":"videos-fr","term_group":0,"term_taxonomy_id":771,"taxonomy":"category","description":"","parent":0,"count":136,"filter":"raw","cat_ID":771,"category_count":136,"category_description":"","cat_name":"Vid\u00e9os","category_nicename":"videos-fr","category_parent":0}],"tag_info":false,"authors":[{"term_id":414,"user_id":11,"is_guest":0,"slug":"restek-corporation","display_name":"Restek Corporation","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/Restek_Favicon_300x300.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts\/42485","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/users\/11"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/comments?post=42485"}],"version-history":[{"count":4,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts\/42485\/revisions"}],"predecessor-version":[{"id":64935,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts\/42485\/revisions\/64935"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/media\/41154"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=42485"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/categories?post=42485"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/tags?post=42485"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/industries-application?post=42485"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/post-badge?post=42485"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/resource-type?post=42485"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/product-library?post=42485"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/resource-technique?post=42485"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/ppma_author?post=42485"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}