{"id":43390,"date":"2020-10-13T00:00:00","date_gmt":"2020-10-13T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/lc-msms-analysis-of-58-antipsychotics-and-antidepressants-in-human-urine\/"},"modified":"2025-12-09T18:44:16","modified_gmt":"2025-12-09T18:44:16","slug":"lc-msms-analysis-of-58-antipsychotics-and-antidepressants-in-human-urine","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/zh-hans\/articles\/cfss3051\/lc-msms-analysis-of-58-antipsychotics-and-antidepressants-in-human-urine","title":{"rendered":"LC-MS\/MS Analysis of 58 Antipsychotics and Antidepressants in Human Urine"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Simple sample preparation procedure and fast, 5.5-min total LC cycle time.<\/li>\n\n\n\n<li>Good chromatographic separation of isobaric compounds.<\/li>\n\n\n\n<li>Optimized method suitable for quantitative analysis.<\/li>\n<\/ul>\n\n\n\n<p>Mental health disorders contribute significantly to worldwide morbidity and mortality, a fact reflected in the growing numbers of new antipsychotic and antidepressant drugs entering the market, as well as the rising rate of prescription. These drugs, which are used to treat a wide range of psychiatric disorders, such as schizophrenia, bipolar disorder, dysthymia, social anxiety disorder, obsessive-compulsive disorder, and chronic pain, have a high potential for abuse. In the forensic setting, detection of these drugs is critical in determining their involvement in intoxications and suicides. In the clinical setting, analysis of antipsychotics and antidepressants in blood or urine is necessary to ensure suitable therapeutic concentration and to monitor patient compliance.<\/p>\n\n\n\n<p>By combining a simple sample preparation procedure and a fast LC-MS\/MS analysis of antipsychotics and antidepressants using a Raptor Biphenyl column, a highly specific and accurate method was established for 58 drugs in human urine. Chromatographic carryover, initially problematic, was resolved by rinsing the injector and needle both externally and internally with a 50:50 methanol:DMSO solution. This, coupled with a highly organic initial mobile phase, ensured the chromatographic separation of isobaric compounds (maprotiline and amitriptyline; protriptyline and nortriptyline) needed for quantitative analysis. Under the conditions shown here, a fast, efficient separation was achieved for the simultaneous LC-MS\/MS analysis of antipsychotics and antidepressants with a 3.5 minute gradient and a 5.5 minute total LC cycle time.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0749.png\" alt=\"Antipsychotics and Antidepressants in Human Urine on Raptor Biphenyl by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_CF0749<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Desmethylolanzapine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/161696-76-0\/Desmethylolanzapine\" target=\"_blank\" rel=\"noopener\">Desmethylolanzapine<\/a><\/td><td class=\"oth\">0.58<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">299.1<\/td><td class=\"oth\">256.1<\/td><td class=\"oth\">198.0<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Phenelzine sulfate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/156-51-4\/Phenelzine sulfate\" target=\"_blank\" rel=\"noopener\">Phenelzine sulfate<\/a><\/td><td class=\"oth\">0.59<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">137.2<\/td><td class=\"oth\">105.1<\/td><td class=\"oth\">77.1<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Olanzapine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/132539-06-1\/Olanzapine\" target=\"_blank\" rel=\"noopener\">Olanzapine<\/a><\/td><td class=\"oth\">0.65<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">313.2<\/td><td class=\"oth\">256.1<\/td><td class=\"oth\">198.1<\/td><\/tr>\n<tr><td class=\"num\">4.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Lamotrigine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/84057-84-1\/Lamotrigine\" target=\"_blank\" rel=\"noopener\">Lamotrigine<\/a><\/td><td class=\"oth\">0.76<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">256.0<\/td><td class=\"oth\">211.1<\/td><td class=\"oth\">145.0<\/td><\/tr>\n<tr><td class=\"num\">5.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Molindone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/7416-34-4\/Molindone\" target=\"_blank\" rel=\"noopener\">Molindone<\/a><\/td><td class=\"oth\">0.81<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">278.1<\/td><td class=\"oth\">100.3<\/td><td class=\"oth\">101.1<\/td><\/tr>\n<tr><td class=\"num\">6.<\/td><td class=\"cmpd\">(+\/-)-Hydroxybupropion<\/td><td class=\"oth\">0.83<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">256.0<\/td><td class=\"oth\">130.2<\/td><td class=\"oth\">166.0<\/td><\/tr>\n<tr><td class=\"num\">7.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 7-Hydroxyquetiapine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/139079-39-3\/7-Hydroxyquetiapine\" target=\"_blank\" rel=\"noopener\">7-Hydroxyquetiapine<\/a><\/td><td class=\"oth\">0.84<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">400.3<\/td><td class=\"oth\">269.0<\/td><td class=\"oth\">208.0<\/td><\/tr>\n<tr><td class=\"num\">8.<\/td><td class=\"cmpd\">Bupropion-D9 (IS)<\/td><td class=\"oth\">0.94<\/td><td class=\"oth\">200<\/td><td class=\"oth\">249.2<\/td><td class=\"oth\">130.9<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">9.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Bupropion\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/34911-55-2\/Bupropion\" target=\"_blank\" rel=\"noopener\">Bupropion<\/a><\/td><td class=\"oth\">0.95<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">240.0<\/td><td class=\"oth\">184.1<\/td><td class=\"oth\">130.2<\/td><\/tr>\n<tr><td class=\"num\">10.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Venlafaxine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/93413-69-5\/Venlafaxine\" target=\"_blank\" rel=\"noopener\">Venlafaxine<\/a><\/td><td class=\"oth\">1.04<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">278.4<\/td><td class=\"oth\">260.4<\/td><td class=\"oth\">121.2<\/td><\/tr>\n<tr><td class=\"num\">11.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Reduced haloperidol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/34104-67-1\/Reduced haloperidol\" target=\"_blank\" rel=\"noopener\">Reduced haloperidol<\/a><\/td><td class=\"oth\">1.07<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">378.1<\/td><td class=\"oth\">359.9<\/td><td class=\"oth\">109.1<\/td><\/tr>\n<tr><td class=\"num\">12.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Milnacipran\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/92623-85-3\/Milnacipran\" target=\"_blank\" rel=\"noopener\">Milnacipran<\/a><\/td><td class=\"oth\">1.09<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">247.2<\/td><td class=\"oth\">100.1<\/td><td class=\"oth\">129.1<\/td><\/tr>\n<tr><td class=\"num\">13.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for N-desmethylmirtazapine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/61337-68-6\/N-desmethylmirtazapine\" target=\"_blank\" rel=\"noopener\">N-desmethylmirtazapine<\/a><\/td><td class=\"oth\">1.10<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">252.1<\/td><td class=\"oth\">195.1<\/td><td class=\"oth\">209.2<\/td><\/tr>\n<tr><td class=\"num\">14.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 9-Hydroxyrisperidone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/144598-75-4\/9-Hydroxyrisperidone\" target=\"_blank\" rel=\"noopener\">9-Hydroxyrisperidone<\/a><\/td><td class=\"oth\">1.15<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">427.3<\/td><td class=\"oth\">207.1<\/td><td class=\"oth\">110.2<\/td><\/tr>\n<tr><td class=\"num\">15.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Mirtazapine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/85650-52-8\/Mirtazapine\" target=\"_blank\" rel=\"noopener\">Mirtazapine<\/a><\/td><td class=\"oth\">1.16<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">266.1<\/td><td class=\"oth\">195.1<\/td><td class=\"oth\">72.1<\/td><\/tr>\n<tr><td class=\"num\">16.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for N-desmethylclozapine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/6104-71-8\/N-desmethylclozapine\" target=\"_blank\" rel=\"noopener\">N-desmethylclozapine<\/a><\/td><td class=\"oth\">1.19<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">313.0<\/td><td class=\"oth\">192.1<\/td><td class=\"oth\">270.0<\/td><\/tr>\n<tr><td class=\"num\">17.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Droperidol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/548-73-2\/Droperidol\" target=\"_blank\" rel=\"noopener\">Droperidol<\/a><\/td><td class=\"oth\">1.24<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">380.1<\/td><td class=\"oth\">122.9<\/td><td class=\"oth\">165.1<\/td><\/tr>\n<tr><td class=\"num\">18.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Clozapine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/5786-21-0\/Clozapine\" target=\"_blank\" rel=\"noopener\">Clozapine<\/a><\/td><td class=\"oth\">1.25<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">328.2<\/td><td class=\"oth\">271.1<\/td><td class=\"oth\">193.1<\/td><\/tr>\n<tr><td class=\"num\">19.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Didesmethyl citalopram\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/62498-69-5\/Didesmethyl citalopram\" target=\"_blank\" rel=\"noopener\">Didesmethyl citalopram<\/a><\/td><td class=\"oth\">1.26<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">297.1<\/td><td class=\"oth\">109.1<\/td><td class=\"oth\">261.9<\/td><\/tr>\n<tr><td class=\"num\">20.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for N-desmethylcitalopram\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/62498-67-3\/N-desmethylcitalopram\" target=\"_blank\" rel=\"noopener\">N-desmethylcitalopram<\/a><\/td><td class=\"oth\">1.27<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">311.1<\/td><td class=\"oth\">109.1<\/td><td class=\"oth\">262.1<\/td><\/tr>\n<tr><td class=\"num\">21.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Escitalopram\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/128196-01-0\/Escitalopram\" target=\"_blank\" rel=\"noopener\">Escitalopram<\/a><\/td><td class=\"oth\">1.28<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">325.3<\/td><td class=\"oth\">109.1<\/td><td class=\"oth\">261.9<\/td><\/tr>\n<tr><td class=\"num\">22.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Fluvoxamine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/54739-18-3\/Fluvoxamine\" target=\"_blank\" rel=\"noopener\">Fluvoxamine<\/a><\/td><td class=\"oth\">1.35<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">319.1<\/td><td class=\"oth\">71.2<\/td><td class=\"oth\">130.1<\/td><\/tr>\n<tr><td class=\"num\">23.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Haloperidol\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/52-86-8\/Haloperidol\" target=\"_blank\" rel=\"noopener\">Haloperidol<\/a><\/td><td class=\"oth\">1.36<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">377.2<\/td><td class=\"oth\">123.0<\/td><td class=\"oth\">95.1<\/td><\/tr>\n<tr><td class=\"num\">24.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Norfluoxetine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/56161-73-0\/Norfluoxetine\" target=\"_blank\" rel=\"noopener\">Norfluoxetine<\/a><\/td><td class=\"oth\">1.37<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">296.3<\/td><td class=\"oth\">134.3<\/td><td class=\"oth\">104.9<\/td><\/tr>\n<tr><td class=\"num\">25.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Isocarboxazid\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/59-63-2\/Isocarboxazid\" target=\"_blank\" rel=\"noopener\">Isocarboxazid<\/a><\/td><td class=\"oth\">1.38<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">232.0<\/td><td class=\"oth\">91.1<\/td><td class=\"oth\">65.2<\/td><\/tr>\n<tr><td class=\"num\">26.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Fluoxetine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/54910-89-3\/Fluoxetine\" target=\"_blank\" rel=\"noopener\">Fluoxetine<\/a><\/td><td class=\"oth\">1.39<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">310.1<\/td><td class=\"oth\">148.0<\/td><td class=\"oth\">115.1<\/td><\/tr>\n<tr><td class=\"num\">27.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Desmethyldoxepin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1225-56-5\/Desmethyldoxepin\" target=\"_blank\" rel=\"noopener\">Desmethyldoxepin<\/a><\/td><td class=\"oth\">1.40<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">266.1<\/td><td class=\"oth\">107.1<\/td><td class=\"oth\">115.0<\/td><\/tr>\n<tr><td class=\"num\">28.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Doxepin\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1668-19-5\/Doxepin\" target=\"_blank\" rel=\"noopener\">Doxepin<\/a><\/td><td class=\"oth\">1.45<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">280.1<\/td><td class=\"oth\">107.2<\/td><td class=\"oth\">77.1<\/td><\/tr>\n<tr><td class=\"num\">29.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Trazodone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/19794-93-5\/Trazodone\" target=\"_blank\" rel=\"noopener\">Trazodone<\/a><\/td><td class=\"oth\">1.46<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">372.3<\/td><td class=\"oth\">176.1<\/td><td class=\"oth\">148.0<\/td><\/tr>\n<tr><td class=\"num\">30.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Oxcarbazepine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/28721-07-5\/Oxcarbazepine\" target=\"_blank\" rel=\"noopener\">Oxcarbazepine<\/a><\/td><td class=\"oth\">1.51<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">253.1<\/td><td class=\"oth\">180.0<\/td><td class=\"oth\">208.1<\/td><\/tr>\n<tr><td class=\"num\">31.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Risperidone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/106266-06-2\/Risperidone\" target=\"_blank\" rel=\"noopener\">Risperidone<\/a><\/td><td class=\"oth\">1.52<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">411.2<\/td><td class=\"oth\">191.0<\/td><td class=\"oth\">110.1<\/td><\/tr>\n<tr><td class=\"num\">32.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Quetiapine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/111974-69-7\/Quetiapine\" target=\"_blank\" rel=\"noopener\">Quetiapine<\/a><\/td><td class=\"oth\">1.75<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">384.2<\/td><td class=\"oth\">253.0<\/td><td class=\"oth\">221.2<\/td><\/tr>\n<tr><td class=\"num\">33.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Asenapine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/85650-56-2\/Asenapine\" target=\"_blank\" rel=\"noopener\">Asenapine<\/a><\/td><td class=\"oth\">1.76<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">286.2<\/td><td class=\"oth\">165.1<\/td><td class=\"oth\">229.1<\/td><\/tr>\n<tr><td class=\"num\">34.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Ziprasidone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/146939-27-7\/Ziprasidone\" target=\"_blank\" rel=\"noopener\">Ziprasidone<\/a><\/td><td class=\"oth\">1.78<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">413.2<\/td><td class=\"oth\">194.1<\/td><td class=\"oth\">130.0<\/td><\/tr>\n<tr><td class=\"num\">35.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Protriptyline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/438-60-8\/Protriptyline\" target=\"_blank\" rel=\"noopener\">Protriptyline<\/a><\/td><td class=\"oth\">1.79<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">264.1<\/td><td class=\"oth\">191.1<\/td><td class=\"oth\">165.2<\/td><\/tr>\n<tr><td class=\"num\">36.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Desipramine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-47-5\/Desipramine\" target=\"_blank\" rel=\"noopener\">Desipramine<\/a><\/td><td class=\"oth\">1.83<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">267.1<\/td><td class=\"oth\">72.1<\/td><td class=\"oth\">193.1<\/td><\/tr>\n<tr><td class=\"num\">37.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Paroxetine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/61869-08-7\/Paroxetine\" target=\"_blank\" rel=\"noopener\">Paroxetine<\/a><\/td><td class=\"oth\">1.85<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">330.2<\/td><td class=\"oth\">192.2<\/td><td class=\"oth\">70.1<\/td><\/tr>\n<tr><td class=\"num\">38.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Iloperidone\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/133454-47-4\/Iloperidone\" target=\"_blank\" rel=\"noopener\">Iloperidone<\/a><\/td><td class=\"oth\">1.85<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">427.1<\/td><td class=\"oth\">261.1<\/td><td class=\"oth\">96.1<\/td><\/tr>\n<tr><td class=\"num\">39.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Duloxetine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/136434-34-9\/Duloxetine\" target=\"_blank\" rel=\"noopener\">Duloxetine<\/a><\/td><td class=\"oth\">1.86<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">298.1<\/td><td class=\"oth\">188.2<\/td><td class=\"oth\">154.1<\/td><\/tr>\n<tr><td class=\"num\">40.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Amoxapine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/14028-44-5\/Amoxapine\" target=\"_blank\" rel=\"noopener\">Amoxapine<\/a><\/td><td class=\"oth\">1.88<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">314.2<\/td><td class=\"oth\">271.0<\/td><td class=\"oth\">193.1<\/td><\/tr>\n<tr><td class=\"num\">41.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Carbamazepine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/298-46-4\/Carbamazepine\" target=\"_blank\" rel=\"noopener\">Carbamazepine<\/a><\/td><td class=\"oth\">1.89<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">237.0<\/td><td class=\"oth\">193.9<\/td><td class=\"oth\">192.0<\/td><\/tr>\n<tr><td class=\"num\">42.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Maprotiline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/10262-69-8\/Maprotiline\" target=\"_blank\" rel=\"noopener\">Maprotiline<\/a><\/td><td class=\"oth\">1.90<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">278.1<\/td><td class=\"oth\">250.2<\/td><td class=\"oth\">191.1<\/td><\/tr>\n<tr><td class=\"num\">43.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Imipramine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-49-7\/Imipramine\" target=\"_blank\" rel=\"noopener\">Imipramine<\/a><\/td><td class=\"oth\">1.91<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">281.1<\/td><td class=\"oth\">86.2<\/td><td class=\"oth\">58.1<\/td><\/tr>\n<tr><td class=\"num\">44.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Nortriptyline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/72-69-5\/Nortriptyline\" target=\"_blank\" rel=\"noopener\">Nortriptyline<\/a><\/td><td class=\"oth\">1.95<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">264.1<\/td><td class=\"oth\">91.1<\/td><td class=\"oth\">115.2<\/td><\/tr>\n<tr><td class=\"num\">45.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Loxapine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1977-10-2\/Loxapine\" target=\"_blank\" rel=\"noopener\">Loxapine<\/a><\/td><td class=\"oth\">1.95<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">328.1<\/td><td class=\"oth\">271.1<\/td><td class=\"oth\">193.0<\/td><\/tr>\n<tr><td class=\"num\">46.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Amitriptyline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-48-6\/Amitriptyline\" target=\"_blank\" rel=\"noopener\">Amitriptyline<\/a><\/td><td class=\"oth\">2.01<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">278.1<\/td><td class=\"oth\">91.1<\/td><td class=\"oth\">202.1<\/td><\/tr>\n<tr><td class=\"num\">47.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Trimipramine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/739-71-9\/Trimipramine\" target=\"_blank\" rel=\"noopener\">Trimipramine<\/a><\/td><td class=\"oth\">2.11<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">295.2<\/td><td class=\"oth\">100.2<\/td><td class=\"oth\">58.2<\/td><\/tr>\n<tr><td class=\"num\">48.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Pimozide\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2062-78-4\/Pimozide\" target=\"_blank\" rel=\"noopener\">Pimozide<\/a><\/td><td class=\"oth\">2.13<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">462.1<\/td><td class=\"oth\">328.0<\/td><td class=\"oth\">109.1<\/td><\/tr>\n<tr><td class=\"num\">49.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Chlorpromazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-53-3\/Chlorpromazine\" target=\"_blank\" rel=\"noopener\">Chlorpromazine<\/a><\/td><td class=\"oth\">2.26<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">319.0<\/td><td class=\"oth\">86.1<\/td><td class=\"oth\">178.2<\/td><\/tr>\n<tr><td class=\"num\">50.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Dehydro aripiprazole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/129722-25-4\/Dehydro aripiprazole\" target=\"_blank\" rel=\"noopener\">Dehydro aripiprazole<\/a><\/td><td class=\"oth\">2.28<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">446.2<\/td><td class=\"oth\">285.0<\/td><td class=\"oth\">98.1<\/td><\/tr>\n<tr><td class=\"num\">51.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Clomipramine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/303-49-1\/Clomipramine\" target=\"_blank\" rel=\"noopener\">Clomipramine<\/a><\/td><td class=\"oth\">2.29<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">315.3<\/td><td class=\"oth\">86.0<\/td><td class=\"oth\">58.0<\/td><\/tr>\n<tr><td class=\"num\">52.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Sertraline\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/79617-96-2\/Sertraline\" target=\"_blank\" rel=\"noopener\">Sertraline<\/a><\/td><td class=\"oth\">2.30<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">306.2<\/td><td class=\"oth\">275.1<\/td><td class=\"oth\">158.9<\/td><\/tr>\n<tr><td class=\"num\">53.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Fluphenazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/69-23-8\/Fluphenazine\" target=\"_blank\" rel=\"noopener\">Fluphenazine<\/a><\/td><td class=\"oth\">2.50<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">438.3<\/td><td class=\"oth\">171.1<\/td><td class=\"oth\">143.2<\/td><\/tr>\n<tr><td class=\"num\">54.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Aripiprazole\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/129722-12-9\/Aripiprazole\" target=\"_blank\" rel=\"noopener\">Aripiprazole<\/a><\/td><td class=\"oth\">2.51<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">448.2<\/td><td class=\"oth\">285.0<\/td><td class=\"oth\">176.1<\/td><\/tr>\n<tr><td class=\"num\">55.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Perphenazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/58-39-9\/Perphenazine\" target=\"_blank\" rel=\"noopener\">Perphenazine<\/a><\/td><td class=\"oth\">2.61<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">404.2<\/td><td class=\"oth\">171.1<\/td><td class=\"oth\">143.2<\/td><\/tr>\n<tr><td class=\"num\">56.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Trifluoperazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/117-89-5\/Trifluoperazine\" target=\"_blank\" rel=\"noopener\">Trifluoperazine<\/a><\/td><td class=\"oth\">2.63<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">408.2<\/td><td class=\"oth\">141.2<\/td><td class=\"oth\">113.1<\/td><\/tr>\n<tr><td class=\"num\">57.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Prochlorperazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/58-38-8\/Prochlorperazine\" target=\"_blank\" rel=\"noopener\">Prochlorperazine<\/a><\/td><td class=\"oth\">2.78<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">374.1<\/td><td class=\"oth\">141.0<\/td><td class=\"oth\">113.1<\/td><\/tr>\n<tr><td class=\"num\">58.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Thiothixene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/5591-45-7\/Thiothixene\" target=\"_blank\" rel=\"noopener\">Thiothixene<\/a><\/td><td class=\"oth\">2.91<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">444.2<\/td><td class=\"oth\">221.2<\/td><td class=\"oth\">235.0<\/td><\/tr>\n<tr><td class=\"num\">59.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Thioridazine\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/50-52-2\/Thioridazine\" target=\"_blank\" rel=\"noopener\">Thioridazine<\/a><\/td><td class=\"oth\">3.18<\/td><td class=\"oth\">2500<\/td><td class=\"oth\">371.2<\/td><td class=\"oth\">126.1<\/td><td class=\"oth\">98.1<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Biphenyl  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A5E?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0749\" rel=\"noopener\">cat.# 9309A5E<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>50 mm x 3.0 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor Biphenyl EXP guard column cartridge 5 mm, 3.0 mm ID, 2.7 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9309A0253?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_CF0749\" rel=\"noopener\">cat.# 9309A0253<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>2500 ng\/mL<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 0.1% formic acid, 5 mM ammonium formate <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Methanol, 0.1% formic acid, 5 mM ammonium formate <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.6<\/td><td>40<\/td><td>60<\/td><\/tr><tr><td>0.20<\/td><td>0.6<\/td><td>40<\/td><td>60<\/td><\/tr><tr><td>3.50<\/td><td>0.6<\/td><td>0<\/td><td>100<\/td><\/tr><tr><td>3.51<\/td><td>0.6<\/td><td>40<\/td><td>60<\/td><\/tr><tr><td>5.50<\/td><td>0.6<\/td><td>40<\/td><td>60<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI+ <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Drug-free human urine (BioIVT) was fortified with 58 analytes at 2500 ng\/mL. Bupropion-D9 was used as the internal standard for quantification of all 58 compounds. The urine sample (50 \u03bcL) was mixed with 15 \u03bcL of IMCSzyme, 20 \u03bcL of reaction buffer, and 10 \u03bcL of internal standard solution (1 \u00b5g\/mL in methanol). Hydrolysis was performed at 45\u00b0C (water bath) for 30 minutes, and then 400 \u00b5L of acetonitrile was added, vortexed to mix, and centrifuged at 4000 rpm for 10 minutes. The supernatant was diluted 2-fold with water and injected for analysis.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_cf0749.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Simultaneous measurement of 58 antipsychotic and antidepressant drugs in human urine can be achieved with a simple sample preparation procedure and a fast 5.5-minute LC-MS\/MS analysis on a Raptor Biphenyl column. This method provides accurate, high-throughput determinations and is suitable for both clinical and forensic monitoring.<\/p>\n","protected":false},"author":11,"featured_media":6517,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[7],"tags":[],"industries-application":[2153,2156,2170,2171],"post-badge":[],"resource-type":[2623],"product-library":[2391,2373,2375,2405],"resource-technique":[2299,2301,2302],"hf_cat_post":[650,2624],"ppma_author":[414],"class_list":["post-43390","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles","industries-application-clinical","industries-application-clinical-toxicology","industries-application-forensics","industries-application-forensics-toxicology","resource-type-featured-application","product-library-lc-columns","product-library-liquid-chromatography-products","product-library-sample-preparation-products","product-library-vials-accessories","resource-technique-liquid-chromatography","resource-technique-mass-spectrometry-ms","resource-technique-ms-ms"],"acf":[],"taxonomy_info":{"category":[{"value":7,"label":"Articles"}],"industries-application":[{"value":2153,"label":"Clinical"},{"value":2156,"label":"Clinical Toxicology"},{"value":2170,"label":"Forensics"},{"value":2171,"label":"Forensics Toxicology"}],"resource-type":[{"value":2623,"label":"Featured Application"}],"product-library":[{"value":2391,"label":"LC Columns"},{"value":2373,"label":"Liquid Chromatography Products"},{"value":2375,"label":"Sample Preparation Products"},{"value":2405,"label":"Vials &amp; 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