{"id":43427,"date":"2020-10-29T14:30:00","date_gmt":"2020-10-29T14:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/characterizing-all-136-tetra-to-octachlorinated-dioxins-and-furans\/"},"modified":"2025-12-05T19:54:33","modified_gmt":"2025-12-05T19:54:33","slug":"characterizing-all-136-tetra-to-octachlorinated-dioxins-and-furans","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/zh-hans\/articoli\/evar3231\/characterizing-all-136-tetra-to-octachlorinated-dioxins-and-furans","title":{"rendered":"Characterizing All 136 Tetra- to Octachlorinated Dioxins and Furans"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Known elution orders for all tetra- through octachlorinated dioxin and furan congeners.<\/li>\n\n\n\n<li>Resolve 14 of 17 tetra- through octachlorine 2,3,7,8-substituted dioxins and furans.<\/li>\n\n\n\n<li>TCDD and TCDF specificity, with a column stable up to 340\u00b0C.<\/li>\n<\/ul>\n\n\n\n<p>Successful analyses of dioxins and furans are critical because of the extremely toxic nature of these compounds. However, confidently resolving the most toxic congeners, 2,3,7,8-substituted tetrachlorinated dibenzodioxin (TCDD) and tetrachlorinated dibenzofuran (TCDF), is often complicated by the presence of the many other possible congeners. Even with high resolution GC\/high resolution MS methods, the proper choice of chromatographic column is essential for separating 2,3,7,8-substituted dioxins and furans from the less toxic congeners and matrix-related compounds.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Complete Column Characterization<\/h2>\n\n\n\n<p>It is rare that a column\u2019s performance is characterized against all possible 136 tetra- through octachlorinated dioxins and furans. These standards are difficult to obtain, and testing can be time consuming. However, here the\u00a0Rtx-Dioxin2\u00a0column is characterized against all 136 compounds using standards from Cambridge Isotope Laboratories, Inc. When compared to industry standard stationary phases, a unique selectivity is observed for the Rtx-Dioxin2 column, and specific resolutions and coelutions are noted. Very few coelutions involving the toxic 2,3,7,8-substituted congeners are observed, making the Rtx-Dioxin2 column an excellent choice for single column analyses of dioxins and furans. (Tables I and II &#8211;\u00a0<em>click images below to enlarge<\/em>)<\/p>\n\n\n\n<p>Figure 1 shows fly ash samples, run under the same chromatographic conditions used to characterize the column. 2,3,7,8-tetrachlorodibenzofuran is not resolved under these conditions. However, the characterization study used simple linear temperature programming, and additional work exploring nonlinear oven programs and different flow parameters yielded better resolution between some congeners, especially 2,3,7,8-TCDF (data available upon request). The value in this work is not necessarily to show complete separation of all the congeners on a single column, but to show where all of the 136 compounds of interest elute, making all possible coelutions known.<\/p>\n\n\n\n<p>The\u00a0Rtx-Dioxin2\u00a0column is an excellent column for the analysis of dioxin and furan congeners. It has a unique selectivity for the toxic congeners, including specificity for 2,3,7,8-TCDD and 2,3,7,8-TCDF. Here we characterized all 136 tetra- through octachlorine dioxins and furans and defined all possible coelutions. While commonly used cyanopropyl columns are limited by a low maximum operating temperature of 240\u00b0C, the Rtx-Dioxin2 column is stable up to 340\u00b0C, extending column lifetime and improving analyses of dioxins and furans.<\/p>\n\n\n\n<p><strong>Table I<\/strong>:\u00a0Retention times (RT) and relative retention times (RRT) for all tetra- through octachlorinated dioxins on an Rtx-Dioxin2 column.<\/p>\n\n\n<style>.kb-image43427_aaf50b-14 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<figure class=\"wp-block-kadence-image kb-image43427_aaf50b-14\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVAR3231-01.jpg\" alt=\"decorative\" class=\"kb-img\" title=\"-\"><\/figure>\n\n\n\n<p><strong>Table II<\/strong>:\u00a0Retention times (RT) and relative retention times (RRT) for all tetra- through octachlorinated furans on an Rtx-Dioxin2 column. <\/p>\n\n\n\n<figure class=\"wp-block-image\"><img decoding=\"async\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure-article-EVAR3231-02.jpg\" alt=\"decorative\" title=\"-\"><\/figure>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1<\/strong>\u00a0\u00a0GC\/HR-MS analysis of tetrachlorinated dioxins in fly ash on an\u00a0Rtx-Dioxin2\u00a0column.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/gc_ev01026.png\" alt=\"GC\/HR-MS analysis of tetrachlorinated dioxins in fly ash on Rtx-Dioxin2\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> GC_EV01026<\/p><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Rtx-Dioxin2, 40 m, 0.18 mm ID, 0.18 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/10759?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_EV01026\" rel=\"noopener\">cat.# 10759<\/a>)<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>TCDDs in fly ash<\/td><\/tr>\n<tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Injection<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1.0 \u00b5L splitless<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Temp.:<\/th><td>280 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\" colspan=\"2\">Oven<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Oven Temp.:<\/th><td>120 &#176;C (hold 1 min) to 160 &#176;C at 10 &#176;C\/min to 320 &#176;C at 4 &#176;C\/min (hold 4 min)<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Carrier Gas<\/th><td>He, constant flow<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow Rate:<\/th><td>1 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>Waters AutoSpec Ultima HRMS (SIM mode)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ionization Mode:<\/th><td>EI <\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-related-skus\"><h4>Related SKUs<\/h4><ul><li>10759<\/li><\/ul><\/div><div class=\"chromatogram-related-searches\"><h4>Related Searches<\/h4><ul><li>HRMS<\/li><li>dioxin<\/li><li>dioxin2<\/li><li>fly ash<\/li><li>furan<\/li><li>tcdd<\/li><li>tcdf<\/li><li>tetrachlorinated dioxin<\/li><\/ul><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/gc_ev01026.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2<\/strong>\u00a0\u00a0GC\/HR-MS analysis of tetrachlorinated furans in fly ash on an\u00a0Rtx-Dioxin2\u00a0column.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/gc_ev01027.png\" alt=\"GC\/HR-MS of Tetrachlorinated Furans in Fly Ash on Rtx-Dioxin2\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> GC_EV01027<\/p><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Rtx-Dioxin2, 40 m, 0.18 mm ID, 0.18 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/10759?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_EV01027\" rel=\"noopener\">cat.# 10759<\/a>)<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>TCDFs in fly ash<\/td><\/tr>\n<tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Injection<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>1.0 \u00b5L splitless<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Temp.:<\/th><td>280 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\" colspan=\"2\">Oven<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Oven Temp.:<\/th><td>120 &#176;C (hold 1 min) to 160 &#176;C at 10 &#176;C\/min to 320 &#176;C at 4 &#176;C\/min (hold 4 min)<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Carrier Gas<\/th><td>He, constant flow<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow Rate:<\/th><td>1 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>Waters AutoSpec Ultima HRMS (SIM mode)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ionization Mode:<\/th><td>EI <\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-related-skus\"><h4>Related SKUs<\/h4><ul><li>10759<\/li><\/ul><\/div><div class=\"chromatogram-related-searches\"><h4>Related Searches<\/h4><ul><li>HRMS<\/li><li>POP<\/li><li>POPs<\/li><li>TCDF<\/li><li>dioxin2<\/li><li>dioxins<\/li><li>fly ash<\/li><li>furans<\/li><li>tetrachlorinated furans<\/li><\/ul><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/gc_ev01027.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">Products Mentioned<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/10758\">                                Catalog No. 10758                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Rtx-Dioxin2 GC Capillary Column, 60 m, 0.25 mm ID, 0.25 \u00b5m<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/10758\">\u67e5\u770b\u4ea7\u54c1<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/10759\">                                Catalog No. 10759                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Rtx-Dioxin2 GC Capillary Column, 40 m, 0.18 mm ID, 0.18 \u00b5m<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/10759\">\u67e5\u770b\u4ea7\u54c1<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The Rtx-Dioxin2 column has a unique selectivity for dioxins and furans, including specificity for 2,3,7,8-TCDD and 2,3,7,8-TCDF. Here we characterize all 136 tetra- through octachlorine dioxins and furans and define all possible coelutions. While commonly used cyanopropyl columns are limited by a low maximum operating temperature of 240\u00b0C, the Rtx-Dioxin2 column is stable up to 340\u00b0C, extending column lifetime and improving the analyses of dioxins and furans.<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[788],"tags":[],"industries-application":[2253],"post-badge":[],"resource-type":[],"product-library":[2516,2528],"resource-technique":[2332],"ppma_author":[590],"class_list":["post-43427","post","type-post","status-publish","format-standard","hentry","category-articoli","industries-application-ambientale","product-library-prodotti-per-gascromatografia","product-library-colonne-gc","resource-technique-gascromatografia-gc"],"acf":[],"taxonomy_info":{"category":[{"value":788,"label":"Articoli"}],"industries-application":[{"value":2253,"label":"Ambientale"}],"product-library":[{"value":2516,"label":"Prodotti per Gascromatografia"},{"value":2528,"label":"Colonne GC"}],"resource-technique":[{"value":2332,"label":"Gascromatografia (GC)"}]},"featured_image_src_large":false,"author_info":{"display_name":"Audrey Crater","author_link":"https:\/\/discover.restek.com\/zh-hans\/author\/audrey-craterrestek-com\/"},"comment_info":0,"category_info":[{"term_id":788,"name":"Articoli","slug":"articoli","term_group":0,"term_taxonomy_id":788,"taxonomy":"category","description":"","parent":0,"count":465,"filter":"raw","cat_ID":788,"category_count":465,"category_description":"","cat_name":"Articoli","category_nicename":"articoli","category_parent":0}],"tag_info":false,"authors":[{"term_id":590,"user_id":0,"is_guest":1,"slug":"jack-cochran","display_name":"Jack Cochran","avatar_url":"https:\/\/secure.gravatar.com\/avatar\/?s=96&d=mm&r=g","0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts\/43427","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/comments?post=43427"}],"version-history":[{"count":3,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts\/43427\/revisions"}],"predecessor-version":[{"id":71808,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts\/43427\/revisions\/71808"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=43427"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/categories?post=43427"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/tags?post=43427"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/industries-application?post=43427"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/post-badge?post=43427"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/resource-type?post=43427"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/product-library?post=43427"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/resource-technique?post=43427"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/ppma_author?post=43427"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}