{"id":43481,"date":"2021-01-13T00:00:00","date_gmt":"2021-01-13T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/simple-large-volume-injection-method-for-trace-level-glyphosate-in-bottled-drinking-water\/"},"modified":"2026-01-28T16:45:15","modified_gmt":"2026-01-28T16:45:15","slug":"simple-large-volume-injection-method-for-trace-level-glyphosate-in-bottled-drinking-water","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/zh-hans\/articles-zh\/evfa3196\/simple-large-volume-injection-method-for-trace-level-glyphosate-in-bottled-drinking-water","title":{"rendered":"Simple Large Volume Injection Method for Trace-Level Glyphosate in Bottled Drinking Water"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Analyze glyphosate without derivatization or ion pairing.<\/li>\n\n\n\n<li>Unique Raptor Polar X column provides good retention and efficient elution, resulting in better peak shape.<\/li>\n\n\n\n<li>Direct large volume injection makes it easy to increase sensitivity at ppt levels.<\/li>\n<\/ul>\n\n\n\n<p>The analysis of glyphosate in water is challenging primarily because it is not retained on reversed-phase columns and its response is often poor due to chelation with metal surfaces along the sample path through the LC-MS\/MS system. Derivatization and ion-pairing reagents are often used to improve performance, but they can be unfriendly to the instrument and require additional sample preparation time. Using a Raptor Polar X column is a better alternative because the novel stationary phase has an affinity for polar anionic compounds, such as glyphosate, and it provides both effective retention and efficient elution.<\/p>\n\n\n\n<p>In the method for glyphosate in water shown here, an ion-exchange mechanism in the column provides enough retention under the initial mobile phase conditions to allow large volumes (500 \u00b5L) of bottled water to be injected, which is a very simple way to improve low-level sensitivity. To achieve the best retention\/elution characteristics, the mobile phase additive and pH were carefully chosen to provide a charge state that leads to adequate retention and optimal sensitivity. In addition, a one-time passivation of the LC system (without an analytical or guard column in the flow path) helped prevent undesirable chelation and a good response was observed at 100 ppt (S:N &gt; 50).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0571.png\" alt=\"Glyphosate in Bottled Drinking Water at 100 ppt on Raptor Polar X by Large Volume Injection Using LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_EV0571<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-6 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">t<sub>R<\/sub> (min)<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/L)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glyphosate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1071-83-6\/Glyphosate\" target=\"_blank\" rel=\"noopener\">Glyphosate<\/a><\/td><td class=\"oth\">6.13<\/td><td class=\"oth\">100<\/td><td class=\"oth\">168.10<\/td><td class=\"oth\">62.95<\/td><td class=\"oth\">78.95<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A32?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0571\" rel=\"noopener\">cat.# 9311A32<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>30 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>30 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Glyphosate (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/32426?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=LC_EV0571\" rel=\"noopener\">cat.# 32426<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Direct injection of bottled drinking water fortified at 100 ppt with glyphosate.<\/td><\/tr><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>500 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water, 0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>1.0<\/td><td>0.5<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>3.0<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>9.0<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>9.01<\/td><td>0.5<\/td><td>5<\/td><td>95<\/td><\/tr><tr><td>10.5<\/td><td>0.5<\/td><td>5<\/td><td>95<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_ev0571.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Trace-level detection of glyphosate can be achieved without complex derivatization or ion-pairing by using direct large volume injection of water on a Raptor Polar X column.<\/p>\n","protected":false},"author":11,"featured_media":6709,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[786],"tags":[],"industries-application":[2191],"post-badge":[],"resource-type":[2623],"product-library":[2427,2409],"resource-technique":[2310,2361],"ppma_author":[414],"class_list":["post-43481","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-articles-zh","industries-application-food-beverage-zh","resource-type-featured-application","product-library-lc-columns-zh","product-library-liquid-chromatography-products-zh","resource-technique-liquid-chromatography-zh","resource-technique-ms-ms-zh"],"acf":[],"taxonomy_info":{"category":[{"value":786,"label":"\u6587\u7ae0"}],"industries-application":[{"value":2191,"label":"\u98df\u54c1\u548c\u996e\u6599"}],"resource-type":[{"value":2623,"label":"Featured 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