{"id":43534,"date":"2025-10-03T21:19:37","date_gmt":"2025-10-03T21:19:37","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/improve-productivity-for-pesticides-analysis-with-resprep-fl-carboprep-plus-spe\/"},"modified":"2025-12-09T16:46:57","modified_gmt":"2025-12-09T16:46:57","slug":"improve-productivity-for-pesticides-analysis-with-resprep-fl-carboprep-plus-spe","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/zh-hans\/artikel\/evss4396\/improve-productivity-for-pesticides-analysis-with-resprep-fl-carboprep-plus-spe","title":{"rendered":"Improve Productivity for Pesticides Analysis with Resprep FL + CarboPrep Plus SPE"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>Dual-bed Florisil and carbon cartridge removes more coextracted matrix components than Florisil alone.<\/li>\n\n\n\n<li>Cleaner extracts mean less GC downtime and &gt;10x higher sample throughput.<\/li>\n<\/ul>\n\n\n\n<p>When analyzing complex soil and water samples for organochlorine pesticides, coextracted matrix components can contaminate the inlet and column, forcing analysts to take instruments offline for time-consuming, unplanned maintenance. Effective sample extract cleanup is the key to keeping your instruments running and generating accurate, reliable data. Pass-through SPE using Florisil cartridges (e.g., EPA Method 3620) is a common cleanup technique, but Resprep FL + CarboPrep Plus SPE cartridges (Resprep FL+CPP)\u2014which contain both Florisil and carbon in a dual-bed, single-cartridge format\u2014provide a much more effective cleanup. Better cleanup means that columns last longer and instruments stay running, which increases sample throughput and lab profitability.<\/p>\n\n\n\n<p>The Florisil used in Resprep FL + CarboPrep Plus SPE cartridges undergoes special moisture-control processing to ensure full activation of the sorbent material and effective removal of polar contaminants. While it is quite good for capturing polar compounds, Florisil does not remove the high molecular weight contaminants that typically foul GC inlets and bring instruments down for maintenance.<\/p>\n\n\n\n<p>Fortunately, the CarboPrep Plus sorbent bed is extremely efficient at removing the most troublesome coextracted matrix components. For example, if humic acids from the sample remain in the final extract, they cause DDT to break down and fail performance requirements for inlet degradation (&lt;15% breakdown) and continuing calibration checks. The high-quality carbon in Resprep FL + CarboPrep Plus SPE cartridges removes these acids, producing visibly cleaner extracts that meet performance requirements longer and allow many more sample injections compared to extracts cleaned with Florisil alone (Figure 1).<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 1:<\/strong> Resprep FL + CarboPrep Plus SPE cleanup allowed &gt;10x more samples to be analyzed compared to Florisil alone.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<style>.kb-image43534_c876e6-80 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43534_c876e6-80\"><figure class=\"aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"587\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure_article_EVSS4396_01-1024x587.jpg\" alt=\"\" class=\"kb-img wp-image-18760\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure_article_EVSS4396_01-1024x587.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure_article_EVSS4396_01-300x172.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure_article_EVSS4396_01-768x440.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure_article_EVSS4396_01-1536x881.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure_article_EVSS4396_01.jpg 1800w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure><\/div>\n\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n\n\n\n<p><em>Experimental Design: DDT (100 ppb) breakdown was measured between every ten injections of a soil sample extract cleaned up using either Florisil SPE or Resprep FL + CarboPrep Plus SPE.<\/em><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Same Procedure, Cleaner Extracts<\/h2>\n\n\n\n<p>Not only do Resprep FL + CarboPrep Plus SPE cartridges remove more coextracted sample matrix contaminants but they also do so using the same solvents and volumes that are commonly used in Florisil cleanup methods. As shown in Figure 2, the same cartridge conditioning; sample loading; and 10 mL hexane:acetone (90:10) elution steps were performed, and the Resprep FL + CarboPrep Plus SPE cartridges produced visibly cleaner final extracts that produced excellent chromatography. In addition to being a drop-in replacement for Florisil cleanup procedures, Reprep FL + CarboPrep Plus SPE cartridges further simplify workflows because the same cartridge effectively cleans most sample types\u2014there is no need to decide whether to use Florisil or carbon for potentially dirty samples.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 2:<\/strong> Resprep FL + CarboPrep Plus SPE cartridges give cleaner final extracts following the same procedure used in a typical Florisil cleanup.<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<style>.kb-image43534_f504c6-14 .kb-image-has-overlay:after{opacity:0.3;}<\/style>\n<div class=\"wp-block-kadence-image kb-image43534_f504c6-14\"><figure class=\"aligncenter size-medium_large\"><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"725\" src=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure_article_EVSS4396_02-768x725.jpg\" alt=\"\" class=\"kb-img wp-image-18766\" title=\"-\" srcset=\"https:\/\/discover.restek.com\/wp-content\/uploads\/figure_article_EVSS4396_02-768x725.jpg 768w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure_article_EVSS4396_02-300x283.jpg 300w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure_article_EVSS4396_02-1024x967.jpg 1024w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure_article_EVSS4396_02-1536x1451.jpg 1536w, https:\/\/discover.restek.com\/wp-content\/uploads\/figure_article_EVSS4396_02.jpg 1800w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure><\/div>\n\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Improve Data Quality and Reporting Accuracy<\/h2>\n\n\n\n<p>In addition to reducing downtime, improving sample throughput, and simplifying workflows, Resprep FL + CarboPrep Plus SPE cartridges help improve analytical performance, so you can be confident that you are reporting accurate results. Powerful, effective removal of coextracted matrix components eliminates sample-based interferences and prevents them from reducing quantitative accuracy. As shown in Table I, recoveries for all compounds in laboratory control samples were within the acceptance range of 80-110%. In addition, due to the presence of Florisil, no breakthrough of trichlorophenol (TCP) was observed. Method requirements are reliably met because the ultra-clean sorbents and tightly controlled manufacturing procedures ensure low background and consistent lot-to-lot performance (Figure 3).<\/p>\n\n\n\n<p><strong>Table I:<\/strong> Effective cleanup with Resprep FL + CarboPrep Plus SPE cartridges helps ensure accurate, reliable results (n=8).<\/p>\n\n\n<figure class=\"wp-block-table\">\n<table>\n<tbody>\n<tr style=\"background-color: #037cab; color: #ffffff;\">\n<td><strong>Compound<\/strong><\/td>\n<td><strong>Concentration (ng\/mL)<\/strong><\/td>\n<td><strong>Average Recovery (%)<\/strong><\/td>\n<td><strong>%RSD<\/strong><\/td>\n<\/tr>\n<tr>\n<td>TCP<\/td>\n<td>100<\/td>\n<td>&lt; 1*<\/td>\n<td>&#8211;<\/td>\n<\/tr>\n<tr style=\"background-color: #ced4d9;\">\n<td>TCMX<\/td>\n<td>20<\/td>\n<td>98<\/td>\n<td>1.8<\/td>\n<\/tr>\n<tr>\n<td>Hexachlorobenzene<\/td>\n<td>5<\/td>\n<td>89<\/td>\n<td>1.3<\/td>\n<\/tr>\n<tr style=\"background-color: #ced4d9;\">\n<td>alpha-BHC<\/td>\n<td>5<\/td>\n<td>100<\/td>\n<td>1.4<\/td>\n<\/tr>\n<tr>\n<td>gamma-BHC<\/td>\n<td>5<\/td>\n<td>99<\/td>\n<td>1.3<\/td>\n<\/tr>\n<tr style=\"background-color: #ced4d9;\">\n<td>beta-BHC<\/td>\n<td>5<\/td>\n<td>102<\/td>\n<td>3.6<\/td>\n<\/tr>\n<tr>\n<td>delta-BHC<\/td>\n<td>5<\/td>\n<td>103<\/td>\n<td>4.6<\/td>\n<\/tr>\n<tr style=\"background-color: #ced4d9;\">\n<td>Heptachlor<\/td>\n<td>5<\/td>\n<td>91<\/td>\n<td>4.1<\/td>\n<\/tr>\n<tr>\n<td>Aldrin<\/td>\n<td>5<\/td>\n<td>98<\/td>\n<td>1.8<\/td>\n<\/tr>\n<tr style=\"background-color: #ced4d9;\">\n<td>Heptachlor epoxide<\/td>\n<td>5<\/td>\n<td>103<\/td>\n<td>1.1<\/td>\n<\/tr>\n<tr>\n<td>gamma-Chlordane<\/td>\n<td>5<\/td>\n<td>105<\/td>\n<td>3.4<\/td>\n<\/tr>\n<tr style=\"background-color: #ced4d9;\">\n<td>alpha-Chlordane<\/td>\n<td>5<\/td>\n<td>96<\/td>\n<td>2.0<\/td>\n<\/tr>\n<tr>\n<td>4,4&#8242;-DDE<\/td>\n<td>10<\/td>\n<td>99<\/td>\n<td>1.6<\/td>\n<\/tr>\n<tr style=\"background-color: #ced4d9;\">\n<td>Endosulfan I<\/td>\n<td>5<\/td>\n<td>101<\/td>\n<td>2.3<\/td>\n<\/tr>\n<tr>\n<td>Dieldrin<\/td>\n<td>10<\/td>\n<td>98<\/td>\n<td>2.6<\/td>\n<\/tr>\n<tr style=\"background-color: #ced4d9;\">\n<td>Endrin<\/td>\n<td>10<\/td>\n<td>97<\/td>\n<td>5.1<\/td>\n<\/tr>\n<tr>\n<td>4,4&#8242;-DDD<\/td>\n<td>10<\/td>\n<td>97<\/td>\n<td>2.9<\/td>\n<\/tr>\n<tr style=\"background-color: #ced4d9;\">\n<td>Endosulfan II<\/td>\n<td>10<\/td>\n<td>99<\/td>\n<td>3.9<\/td>\n<\/tr>\n<tr>\n<td>4,4&#8242;-DDT<\/td>\n<td>10<\/td>\n<td>97<\/td>\n<td>2.2<\/td>\n<\/tr>\n<tr style=\"background-color: #ced4d9;\">\n<td>Endrin aldehyde<\/td>\n<td>10<\/td>\n<td>92<\/td>\n<td>4.6<\/td>\n<\/tr>\n<tr>\n<td>Methoxychlor<\/td>\n<td>50<\/td>\n<td>95<\/td>\n<td>4.1<\/td>\n<\/tr>\n<tr style=\"background-color: #ced4d9;\">\n<td>Endosulfan sulfate<\/td>\n<td>10<\/td>\n<td>100<\/td>\n<td>2.1<\/td>\n<\/tr>\n<tr>\n<td>Endrin ketone<\/td>\n<td>10<\/td>\n<td>98<\/td>\n<td>1.3<\/td>\n<\/tr>\n<tr style=\"background-color: #ced4d9;\">\n<td>DCB<\/td>\n<td>20<\/td>\n<td>101<\/td>\n<td>2.4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/figure>\n\n\n<p>*Meets requirement of &lt;5% breakthrough.<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><strong>Figure 3:<\/strong> Resprep FL + CarboPrep Plus cartridges have consistently low background due to proprietary manufacturing procedures and strict quality controls. (Eight injections per lot are shown.)<\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image regular-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/gc_ev1523.png\" alt=\"Chromtographic Background of Resprep FL + CarboPrep Plus SPE Cartridges\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> GC_EV1523<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-lg-4 col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 50px\">t<sub>R<\/sub> (min)<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for 2,4,5,6-Tetrachloro-m-xylene\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/877-09-8\/2,4,5,6-Tetrachloro-m-xylene\" target=\"_blank\" rel=\"noopener\">2,4,5,6-Tetrachloro-m-xylene<\/a><\/td><td class=\"oth\">5.65<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Biphenyl, decachloro-\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/2051-24-3\/Biphenyl, decachloro-\" target=\"_blank\" rel=\"noopener\">Biphenyl, decachloro-<\/a><\/td><td class=\"oth\">10.00<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Rtx-CLPesticides, 30 m, 0.32 mm ID, 0.32 \u00b5m (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/11141?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_EV1523\" rel=\"noopener\">cat.# 11141<\/a>)<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td><\/td><\/tr><tr><td><\/td><td>2,4,5,6-Tetrachloro-meta-xylene (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/32027?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_EV1523\" rel=\"noopener\">cat.# 32027<\/a>)<\/td><\/tr><tr><td><\/td><td>Decachlorobiphenyl (BZ #209) (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/32029?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_EV1523\" rel=\"noopener\">cat.# 32029<\/a>)<\/td><\/tr>\n<tr><th class=\"sub conditions_header\" scope=\"row\">Diluent:<\/th><td>Hexane<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Conc.:<\/th><td>5 ng\/\u00b5L<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Injection<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>4 \u00b5L pulsed splitless<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Liner:<\/th><td>Topaz single taper inlet liner w\/wool (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/23303?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_EV1523\" rel=\"noopener\">cat.# 23303<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Temp.:<\/th><td>250 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pulse Pressure:<\/th><td>35 psi (241.3kPa)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pulse Time:<\/th><td>0.74  min<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Purge Flow:<\/th><td>50 mL\/min<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\" colspan=\"2\">Oven<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Oven Temp.:<\/th><td>70 &#176;C (hold 0.5 min) to 320 &#176;C at 25 &#176;C\/min (hold 2 min)<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Carrier Gas<\/th><td>He, constant flow<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Flow Rate:<\/th><td>3.5 mL\/min<\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>Micro-ECD @ 330 \u00b0C<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Make-up Gas Flow Rate:<\/th><td>60 mL\/min<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Make-up Gas Type:<\/th><td>N<sub>2<\/sub> <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Data Rate:<\/th><td>50 Hz<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>Agilent 7890B GC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Conditioned a Resprep FL + CarboPrep Plus SPE cartridge (<a target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/28899?utm_source=chromatograms&amp;utm_medium=link&amp;utm_campaign=GC_EV1523\" rel=\"noopener\">cat.# 28899<\/a>) by adding one cartridge volume of hexane:acetone (90:10) and letting it stand for five minutes before drawing the solvent down to frit level. Then, 1 mL of sample extract was loaded onto the cartridge and eluted with 9 mL of hexane:acetone (90:10).<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/gc_ev1523.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<p><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><em>Enhance Your Florisil Cleanup\u2014Switch to Resprep FL+CPP SPE Cartridges to Reduce Downtime, Improve Data Quality, and Increase Lab Productivity!<\/em><\/h3>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>With both Florisil and carbon sorbents in a single cartridge, Resprep FL + CarboPrep Plus SPE cartridges help reduce instrument downtime and increase lab productivity.<\/p>\n","protected":false},"author":11,"featured_media":6817,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[787],"tags":[],"industries-application":[2232,2230,2668,2235],"post-badge":[],"resource-type":[566],"product-library":[2479,2480,2488,2492,2494,2482,2483,2510],"resource-technique":[2324,2329],"hf_cat_post":[650],"ppma_author":[414],"class_list":["post-43534","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-artikel","industries-application-trinkwasser-abwasseruntersuchungen","industries-application-umweltbereich","industries-application-semivolatiles","industries-application-testung-von-boden-sediment-organismen","resource-type-sample-handling-preparation","product-library-produkte-zur-luft-gasprobenahme","product-library-gaschromatographie-produkte","product-library-gasstandards-zubehor","product-library-gc-saulen","product-library-inlet-zubehor","product-library-referenzstandards","product-library-produkte-zur-probenvorbereitung","product-library-spe-sle-produkte","resource-technique-gaschromatographie-gc","resource-technique-festphasenextraktion-spe"],"acf":[],"taxonomy_info":{"category":[{"value":787,"label":"Artikel"}],"industries-application":[{"value":2232,"label":"Trinkwasser- und Abwasseruntersuchungen"},{"value":2230,"label":"Umweltbereich"},{"value":2668,"label":"Semivolatiles"},{"value":2235,"label":"Testung von Boden, Sediment, Organismen"}],"resource-type":[{"value":566,"label":"Sample Handling &amp; 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