{"id":43645,"date":"2020-10-26T14:30:00","date_gmt":"2020-10-26T14:30:00","guid":{"rendered":"https:\/\/discover.restek.com\/uncategorized\/the-effects-of-lc-particle-choice-on-column-performance-raptor-1-8-m-superficially-porous-particles-spp-for-uhplc-analyses\/"},"modified":"2026-01-28T16:01:20","modified_gmt":"2026-01-28T16:01:20","slug":"the-effects-of-lc-particle-choice-on-column-performance-raptor-1-8-m-superficially-porous-particles-spp-for-uhplc-analyses","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/zh-hans\/articles-fr\/gnar2803\/the-effects-of-lc-particle-choice-on-column-performance-raptor-1-8-m-superficially-porous-particles-spp-for-uhplc-analyses","title":{"rendered":"The Effects of LC Particle Choice on Column Performance: Raptor 1.8 \u00b5m Superficially Porous Particles (SPP) for UHPLC Analyses"},"content":{"rendered":"\n

Superficially porous particles (commonly referred to as SPP or \u201ccore-shell\u201d particles) have been proven to provide fast and efficient LC separations. These particles feature a solid, impermeable core enveloped by a thin, porous layer of silica that offers significantly higher efficiency and sensitivity than traditional fully porous particles (FPP).<\/p>\n\n\n\n

At launch, Raptor SPP LC columns were touted for their selectivity and for their ability to help customers achieve faster separations with their existing HPLC instruments. With the launch of Raptor 1.8 \u00b5m columns, this superior Raptor performance has been unleashed on your UHPLC analyses. But, what exactly does that mean? In this technical note, we will explore the benefits of using Raptor 1.8 \u00b5m LC columns in your UHPLC instrument with a focus on peak capacity, column lifetime, and selectivity.<\/p>\n\n\n\n

Handling the Pressure: Peak Capacity<\/h2>\n\n\n\n

Peak capacity is a valuable measure of system efficiency and is defined by the number of peaks that can be separated by a given chromatographic system and conditions. Raptor 1.8 \u00b5m SPP columns offer exceptional peak capacity in UHPLC conditions, far surpassing the performance of fully porous or larger-diameter superficially porous particles. Because Raptor 1.8 \u00b5m columns have been designed and extensively tested to prove they can offer the highest levels of peak capacity and resolution, they are especially desirable for complex analyses performed with UHPLC.<\/p>\n\n\n\n

The extremely complex samples involved with unknown protein identification, for example, require very high efficiency, which results in high peak capacity. As shown in Figure 1 with the analysis of tryptic digested proteins, the selectivity and ruggedness of Raptor columns combined with these small 1.8 \u00b5m superficially porous particles designed for high-pressure use allow chromatographic conditions to be tailored to produce incredibly high peak capacities on UHPLC instrumentation.<\/p>\n\n\n

Figure 1:<\/strong>\u00a0Maximize peak capacity and efficiency, even for complex samples, by switching to Raptor 1.8 \u00b5m columns on UHPLC instrumentation.<\/div>
\n
\"Maximizing<\/div>

LC_BA0354<\/p>

Conditions<\/h4>
Column<\/th>Raptor ARC-18 (cat.# 9314212<\/a>)<\/td><\/tr>
Dimensions:<\/th>100 mm x 2.1 mm ID<\/td><\/tr>
Particle Size:<\/th>1.8 \u00b5m<\/td><\/tr>
Pore Size:<\/th>90 \u00c5<\/td><\/tr>
<\/td>
Temp.:<\/th>60 \u00b0C<\/td><\/tr>
Standard\/Sample<\/th><\/td><\/tr>
Diluent:<\/th>0.1% TFA in water<\/td><\/tr>
Conc.:<\/th>1,750 \u00b5g\/mL total BSA concentration before digestion<\/td><\/tr><\/td><\/tr>
Inj. Vol.:<\/th>10 \u00b5L <\/td><\/tr>
Mobile Phase<\/th><\/td><\/tr>
A:<\/th>0.1% Trifluoroacetic acid in water <\/td><\/tr>
B:<\/th>0.1% Trifluoroacetic acid in acetonitrile <\/td><\/tr>
<\/td>
Time (min)<\/th>%A<\/th>%B<\/th><\/tr><\/thead>
0.00<\/td>97<\/td>3<\/td><\/tr>
15<\/td>65<\/td>35<\/td><\/tr>
18<\/td>65<\/td>35<\/td><\/tr><\/tbody><\/table><\/td><\/tr>
Flow:<\/th> mL\/min<\/td><\/tr><\/table>
Detector<\/th>PDA @ 207 nm<\/td><\/tr>
Instrument<\/th>UHPLC<\/td><\/tr>
Notes<\/th>A flow rate of 1.1 mL\/min was used for all columns, except for the 1.8 \u03bcm fully porous column. The flow rate used for that column was 0.8 min\/mL, in order to prevent excessive backpressure.<\/td><\/tr><\/table><\/div><\/div><\/div>

Related SKUs<\/h4>