{"id":60503,"date":"2025-11-07T14:02:45","date_gmt":"2025-11-07T14:02:45","guid":{"rendered":"https:\/\/discover.restek.com\/?p=60503"},"modified":"2026-01-08T14:21:41","modified_gmt":"2026-01-08T14:21:41","slug":"analysis-of-%ce%b4-8-thc-%ce%b4-9-thc-and-isomer-metabolites-in-whole-blood-by-lc-ms-ms","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/zh-hans\/posters-presentations\/gnot5306\/analysis-of-%ce%b4-8-thc-%ce%b4-9-thc-and-isomer-metabolites-in-whole-blood-by-lc-ms-ms","title":{"rendered":"Analysis of \u0394-8-THC, \u0394-9-THC, and Isomer Metabolites in Whole Blood by LC-MS\/MS"},"content":{"rendered":"\n<p>The testing of whole blood samples for tetrahydrocannabinol (\u0394-9-THC) consumption is routine and has been around for many decades. As more isomers of \u0394-9-THC become available on the market, testing becomes more complicated, and novel methods are needed to achieve isomeric resolution. One such isomer, \u0394-8-THC, is federally unregulated in the United States and readily available for purchase in many stores. This compound forms its own hydroxylated and carboxylated metabolites, (11-OH-\u0394-8-THC and \u0394-8-THC-COOH), that must be chromatographically resolved from their isomeric metabolites.<\/p>\n\n\n\n<p>An LC-MS\/MS method was successfully developed on a Raptor FluoroPhenyl 100 x 3.0 mm, 2.7 \u03bcm column for reliable and accurate testing of \u0394-8\/9-THC isomers and isomer metabolites in whole blood. The method was determined to be quick, rugged, and sensitive enough to meet reporting guidelines for clinical and forensic toxicology laboratories.<\/p>\n\n\n\n<div data-wp-interactive=\"core\/file\" class=\"wp-block-file\"><object data-wp-bind--hidden=\"!state.hasPdfPreview\" hidden class=\"wp-block-file__embed\" data=\"https:\/\/discover.restek.com\/wp-content\/uploads\/poster-msacl-2024-Analysis-THC-Isomers-in-Blood.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of poster-msacl-2024-Analysis THC Isomers in Blood.\"><\/object><a id=\"wp-block-file--media-6a25ecc9-90da-45c9-9bde-3713b8946780\" href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/poster-msacl-2024-Analysis-THC-Isomers-in-Blood.pdf\">poster-msacl-2024-Analysis THC Isomers in Blood<\/a><a href=\"https:\/\/discover.restek.com\/wp-content\/uploads\/poster-msacl-2024-Analysis-THC-Isomers-in-Blood.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-6a25ecc9-90da-45c9-9bde-3713b8946780\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>The testing of whole blood samples for tetrahydrocannabinol (\u0394-9-THC) consumption is routine and has been around for many decades. As more isomers of \u0394-9-THC become available on the market, testing becomes more complicated, and novel methods are needed to achieve isomeric resolution. One such isomer, \u0394-8-THC, is federally unregulated in the United States and readily available for purchase in many stores. This compound forms its own hydroxylated and carboxylated metabolites, (11-OH-\u0394-8-THC and \u0394-8-THC-COOH), that must be chromatographically resolved from their isomeric metabolites.  <\/p>\n<p>An LC-MS\/MS method was successfully developed on a Raptor FluoroPhenyl 100 x 3.0 mm, 2.7 \u03bcm column for reliable and accurate testing of \u0394-8\/9-THC isomers and isomer metabolites in whole blood. The method was determined to be quick, rugged, and sensitive enough to meet reporting guidelines for clinical and forensic toxicology laboratories.<\/p>\n","protected":false},"author":13,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_kadence_starter_templates_imported_post":false,"_kad_post_transparent":"","_kad_post_title":"","_kad_post_layout":"","_kad_post_sidebar_id":"","_kad_post_content_style":"","_kad_post_vertical_padding":"","_kad_post_feature":"","_kad_post_feature_position":"","_kad_post_header":false,"_kad_post_footer":false,"footnotes":""},"categories":[12],"tags":[],"industries-application":[],"post-badge":[],"resource-type":[],"product-library":[],"resource-technique":[],"ppma_author":[432,579],"class_list":["post-60503","post","type-post","status-publish","format-standard","hentry","category-posters-presentations"],"acf":[],"taxonomy_info":{"category":[{"value":12,"label":"Posters &amp; Presentations"}]},"featured_image_src_large":false,"author_info":{"display_name":"Haley Berkland, MS","author_link":"https:\/\/discover.restek.com\/zh-hans\/author\/haley-berkland-ms\/"},"comment_info":0,"category_info":[{"term_id":12,"name":"Posters &amp; Presentations","slug":"posters-presentations","term_group":0,"term_taxonomy_id":12,"taxonomy":"category","description":"","parent":0,"count":118,"filter":"raw","cat_ID":12,"category_count":118,"category_description":"","cat_name":"Posters &amp; Presentations","category_nicename":"posters-presentations","category_parent":0}],"tag_info":false,"authors":[{"term_id":432,"user_id":13,"is_guest":0,"slug":"haley-berkland-ms","display_name":"Haley Berkland, MS","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-berkland-haley.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/people-berkland-haley.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""},{"term_id":579,"user_id":47,"is_guest":0,"slug":"jamie-l-york-phd","display_name":"Jamie L. York, PhD","avatar_url":{"url":"https:\/\/discover.restek.com\/wp-content\/uploads\/york-jamie-1000x1000-1-e1765483005988.jpg","url2x":"https:\/\/discover.restek.com\/wp-content\/uploads\/york-jamie-1000x1000-1-e1765483005988.jpg"},"0":null,"1":"","2":"","3":"","4":"","5":"","6":"","7":"","8":""}],"_links":{"self":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts\/60503","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/comments?post=60503"}],"version-history":[{"count":2,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts\/60503\/revisions"}],"predecessor-version":[{"id":60730,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/posts\/60503\/revisions\/60730"}],"wp:attachment":[{"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/media?parent=60503"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/categories?post=60503"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/tags?post=60503"},{"taxonomy":"industries-application","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/industries-application?post=60503"},{"taxonomy":"post-badge","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/post-badge?post=60503"},{"taxonomy":"resource-type","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/resource-type?post=60503"},{"taxonomy":"product-library","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/product-library?post=60503"},{"taxonomy":"resource-technique","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/resource-technique?post=60503"},{"taxonomy":"author","embeddable":true,"href":"https:\/\/discover.restek.com\/zh-hans\/wp-json\/wp\/v2\/ppma_author?post=60503"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}