{"id":72084,"date":"2021-01-12T00:00:00","date_gmt":"2021-01-12T00:00:00","guid":{"rendered":"https:\/\/discover.restek.com\/?p=72084"},"modified":"2026-01-28T15:41:32","modified_gmt":"2026-01-28T15:41:32","slug":"chinese-fast-and-rugged-direct-analysis-of-polar-pesticides-in-spinach","status":"publish","type":"post","link":"https:\/\/discover.restek.com\/zh-hans\/articles-zh\/fsfa3215-cn\/chinese-fast-and-rugged-direct-analysis-of-polar-pesticides-in-spinach","title":{"rendered":"Analyse rapide et robuste de pesticides polaires dans les \u00e9pinards"},"content":{"rendered":"\n<ul class=\"wp-block-list\">\n<li>\u65e0\u9700\u6267\u884c\u8d39\u65f6\u7684\u884d\u751f\u5316\u6216\u79bb\u5b50\u914d\u5bf9\u6b65\u9aa4\uff0c\u4e5f\u65e0\u9700\u957f\u65f6\u95f4\u7b49\u5f85\u8272\u8c31\u67f1\u5e73\u8861\u3002<\/li>\n\n\n\n<li>\u72ec\u7279\u7684\u56fa\u5b9a\u76f8\u63d0\u4f9b\u4e86\u826f\u597d\u7684\u4fdd\u7559\u548c\u6709\u6548\u7684\u6d17\u8131\uff0c\u53ef\u5f62\u6210\u66f4\u597d\u7684\u5cf0\u5f62\u3002<\/li>\n\n\n\n<li>\u7a33\u5b9a\u7684\u8272\u8c31\u67f1\u6027\u80fd\u53ef\u786e\u4fdd\u8272\u8c31\u5206\u6790\u7684\u4e00\u81f4\u6027\u548c\u53ef\u9760\u7684\u7ed3\u679c\u3002<\/li>\n<\/ul>\n\n\n\n<p>\u5bf9\u6781\u6027\u519c\u836f\uff08\u5982\u8349\u7518\u81a6\u53ca\u5176\u4e3b\u8981\u4ee3\u8c22\u7269 AMPA \u548c\u8349\u94f5\u81a6\uff09\u8fdb\u884c\u76f4\u63a5\u5206\u6790\u662f\u5f88\u56f0\u96be\u7684\uff0c\u56e0\u4e3a\u5b83\u4eec\u4e0d\u80fd\u5f88\u597d\u5730\u4fdd\u7559\u5728\u53cd\u76f8\u67f1\u4e0a\uff0c\u5e76\u4e14\u4e0e LC-MS\/MS \u4e2d\u7684\u91d1\u5c5e\u8868\u9762\u7684\u87af\u5408\u53ef\u663e\u8457\u964d\u4f4e\u54cd\u5e94\u3002\u884d\u751f\u5316\u548c\u79bb\u5b50\u914d\u5bf9\u8bd5\u5242\u901a\u5e38\u7528\u4e8e\u6539\u5584\u6027\u80fd\uff0c\u4f46\u5b83\u4eec\u9700\u8981\u989d\u5916\u7684\u6837\u54c1\u5236\u5907\u65f6\u95f4\uff0c\u5e76\u4e14\u53ef\u80fd\u4ea7\u751f\u4e0d\u826f\u7684\u526f\u4f5c\u7528\uff08\u6c89\u6dc0\u3001\u8f83\u957f\u7684\u5e73\u8861\u65f6\u95f4\u3001\u7cfb\u7edf\u6c61\u67d3\u7b49\uff09\u3002<\/p>\n\n\n\n<p>\u4f7f\u7528 Raptor Polar X \u67f1\u662f\u4e00\u4e2a\u66f4\u597d\u7684\u89e3\u51b3\u65b9\u6848\uff0c\u56e0\u4e3a\u5176\u65b0\u7684\u56fa\u5b9a\u76f8\u63d0\u4f9b\u4e86\u4e0e\u6781\u6027\u9634\u79bb\u5b50\u5316\u5408\u7269\u66f4\u5f3a\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u5b83\u5177\u6709\u6df7\u5408\u4fdd\u7559\u6a21\u5f0f\uff08HILIC \u548c\u79bb\u5b50\u4ea4\u6362\uff09\uff0c\u5e76\u63d0\u4f9b\u5f3a\u4fdd\u7559\uff0c\u4f46\u4ecd\u7136\u5141\u8bb8\u5206\u6790\u7269\u901a\u8fc7\u7b80\u5355\u7684\u6d41\u52a8\u76f8\u53d8\u5316\u5feb\u901f\u6d17\u8131\u4e3a\u5bf9\u79f0\u5cf0\u3002\u5728\u8fd9\u6b21\u5bf9\u83e0\u83dc\u7684\u6781\u6027\u519c\u836f\u7684\u5206\u6790\u4e2d\uff0c\u8fd9\u4e09\u79cd\u5316\u5408\u7269\u7684\u8272\u8c31\u6027\u80fd\u90fd\u5f88\u597d\uff0c\u603b\u5206\u6790\u65f6\u95f4\u53ea\u6709 8 \u5206\u949f\u3002\u6b64\u5916\uff0c\u901a\u8fc7\u6570\u767e\u6b21\u7684\u57fa\u8d28\u6ce8\u5c04\uff0c\u5cf0\u5f62\u548c\u4fdd\u7559\u65f6\u95f4\u4ecd\u80fd\u4fdd\u6301\u7a33\u5b9a\uff0c\u5e76\u4e14\u7b80\u5355\u5730\u66f4\u6362\u4fdd\u62a4\u67f1\u5373\u53ef\u6062\u590d\u5168\u90e8\u6027\u80fd\u3002\u8fd9\u79cd\u5feb\u901f\u3001\u7b80\u5355\u3001\u6709\u6548\u7684\u5de5\u4f5c\u6d41\u7a0b\u662f\u5bf9\u6781\u6027\u519c\u836f\u7684\u76f4\u63a5\u5206\u6790\u611f\u5174\u8da3\u7684\u98df\u54c1\u5b9e\u9a8c\u5ba4\u7684\u4e00\u4e2a\u5f88\u597d\u7684\u9009\u62e9\uff0c\u5b83\u4e0d\u9700\u8981\u590d\u6742\u548c\u8017\u65f6\u7684\u7a0b\u5e8f\u3002<\/p>\n\n\n<div class=\"wp-block-custom-chromatogram-article-top\"><div class=\"chromatogram-article-placeholder\"><div class=\"figure-heading\"><\/div><div class='chromatogram-article-inner-full'><div class=\"chromatogram-article-inner\">\n<div class=\"wp-block-custom-chromatogram-article\"><div class=\"wp-block-custom-chromatogram-article\"><div class=\"chromatogram-image wide-image\"><img decoding=\"async\" src=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0547.png\" alt=\"Polar Pesticides in Spinach on Raptor Polar X by LC-MS\/MS\" title=\"-\"><\/div><p class=\"article-id\" style=\"text-align:center\"> LC_FS0547<\/p><div class=\"chromatogram-peaks\"><h4>Peaks<\/h4><table class=\"peaks col-12\">\n<thead><tr><th><\/th><th style=\"text-align: left;width: 75px\">Peaks<\/th><th style=\"text-align: center;width: 75px\">Conc.<br \/>(ng\/mL)<\/th><th style=\"text-align: center;width: 75px\">Precursor Ion<\/th><th style=\"text-align: center;width: 75px\">Product Ion 1<\/th><th style=\"text-align: center;width: 75px\">Product Ion 2<\/th><th style=\"text-align: center;width: 75px\">Product Ion 3<\/th><\/tr><\/thead>\n<tbody><tr><td class=\"num\">1.<\/td><td class=\"cmpd\">AMPA<\/td><td class=\"oth\">100<\/td><td class=\"oth\">110.10<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">63.10<\/td><td class=\"oth\">81.10<\/td><\/tr>\n<tr><td class=\"num\">2.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glufosinate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/51276-47-2\/Glufosinate\" target=\"_blank\" rel=\"noopener\">Glufosinate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">180.20<\/td><td class=\"oth\">85.15<\/td><td class=\"oth\">95.10<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<tr><td class=\"num\">3.<\/td><td class=\"cmpd\"><a class=\"cmpd_link\" title=\"View compound information for Glyphosate\" href=\"https:\/\/ez.restek.com\/compound\/view\/en\/1071-83-6\/Glyphosate\" target=\"_blank\" rel=\"noopener\">Glyphosate<\/a><\/td><td class=\"oth\">100<\/td><td class=\"oth\">168.10<\/td><td class=\"oth\">63.05<\/td><td class=\"oth\">79.05<\/td><td class=\"oth\">&#8211;<\/td><\/tr>\n<\/tbody><\/table><\/div><div class=\"chromatogram-conditions\"><h4>Conditions<\/h4><div class=\"conditions-container container-fluid\"><div class=\"row\"><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Column<\/th><td>Raptor Polar X  (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A32\" rel=\"noopener\">cat.# 9311A32<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Dimensions:<\/th><td>30 mm x 2.1 mm ID<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Particle Size:<\/th><td>2.7 \u00b5m<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Pore Size:<\/th><td>90 \u00c5<\/td><\/tr><tr><td><\/td><tr><th class=\"sub conditions_header\" scope=\"row\">Guard Column:<\/th><td>Raptor Polar X guard column cartridge 5 mm, 2.1 mm ID, 2.7 \u00b5m (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/9311A0252\" rel=\"noopener\">cat.# 9311A0252<\/a>)<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Temp.:<\/th><td>35 \u00b0C<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Standard\/Sample<\/th><td>Spinach extract<\/td><\/tr>\n<td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Inj. Vol.:<\/th><td>2 \u00b5L <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Mobile Phase<\/th><td><\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">A:<\/th><td>Water,  0.5% formic acid <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">B:<\/th><td>Acetonitrile, 0.5% formic acid <\/td><\/tr><tr><td><\/td><td><table class=\"cgram_ramp\"><thead><tr><th>Time (min)<\/th><th>Flow (mL\/min)<\/th><th>%A<\/th><th>%B<\/th><\/tr><\/thead><tbody><tr><td>0.00<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>5.0<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.5<\/td><td>0.5<\/td><td>90<\/td><td>10<\/td><\/tr><tr><td>6.51<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><tr><td>8<\/td><td>0.5<\/td><td>35<\/td><td>65<\/td><\/tr><\/tbody><\/table><\/td><\/tr><\/table><table class=\"conditions col-lg-6 col-12\"><tr><th class=\"conditions_header\" scope=\"row\">Detector<\/th><td>MS\/MS<\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Ion Mode:<\/th><td>ESI- <\/td><\/tr><tr><th class=\"sub conditions_header\" scope=\"row\">Mode:<\/th><td>MRM <\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Instrument<\/th><td>UHPLC<\/td><\/tr><tr class=\"cgram_header_row\"><th class=\"conditions_header\" scope=\"row\">Sample Preparation<\/th><td>Frozen spinach was added to a Blixer processor with dry ice (3:1-4:1 ratio) and then ground into a very fine powder. The homogenate was placed into the freezer immediately. A 5.0 gram sample of the spinach powder was weighed into a 50 mL centrifuge tube (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/25846\" rel=\"noopener\">cat.# 25846<\/a>). According to the QuPPe method (Quick Polar Pesticides Method), 5.0 mL of methanol with 1.0% formic acid was added into the centrifuge tube. The tube was shaken by hand for 1 min and then by a mechanical shaker vigorously for 5 min. After centrifuging for 10 min at 4200 rpm, the supernatant was filtered through a 0.22 \u03bcm filter (<a class=\"ga-cgram-prod-link\" target=\"_blank\" href=\"https:\/\/www.restek.com\/p\/23984\" rel=\"noopener\">cat.# 23984<\/a>). The final extract was fortified with the AMPA, glufosinate, and glyphosate at a final concentration of 100 ng\/mL.<\/td><\/tr><\/table><\/div><\/div><\/div><div class=\"chromatogram-related-skus\"><h4>Related SKUs<\/h4><ul><li>9311A0252<\/li><li>9311A32<\/li><li>25846<\/li><li>23984<\/li><\/ul><\/div><div class=\"chromatogram-pdf-link\"><a href=\"https:\/\/ez.restek.com\/images\/cgram\/lc_fs0547.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"18\" height=\"18\" viewBox=\"0 0 18 18\"><g data-name=\"Group 2996\"><path data-name=\"Rectangle 1246\" d=\"M0 0h18v18H0z\" style=\"fill: none;\"><\/path><\/g><g data-name=\"Group 2997\"><path data-name=\"Path 729\" d=\"M13.412 11.4v2.017H5.345V11.4H4v2.017a1.349 1.349 0 0 0 1.345 1.345h8.068a1.349 1.349 0 0 0 1.345-1.345V11.4zm-.672-2.694-.948-.948-1.741 1.735V4H8.706v5.493L6.965 7.758l-.948.948 3.361 3.361z\" transform=\"translate(-.437 -.414)\" style=\"fill: rgb(13, 123, 196);\"><\/path><\/g><\/svg>Download PDF<\/a><\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n        <div class=\"cpb\">\n            <h3 class=\"cpb-heading\">\u76f8\u5173\u4ea7\u54c1<\/h3>\n            <hr class=\"cpb-heading-underline\" \/>\n            <div class=\"cpb-list\">\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9311A32\">                                \u8d27\u53f7\uff1a 9311A32                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor Polar X, 2.7 \u00b5m, 30 x 2.1 mm LC Column<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9311A32\">\u67e5\u770b\u4ea7\u54c1<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9311A0252\">                                \u8d27\u53f7\uff1a 9311A0252                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Raptor Polar X, 2.7 \u00b5m, 5 x 2.1 mm EXP Guard Column Cartridge, 3-pk.<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/9311A0252\">\u67e5\u770b\u4ea7\u54c1<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/25846\">                                \u8d27\u53f7\uff1a 25846                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">Empty Centrifuge Tubes, 50 mL, Polypropylene w\/Cap, 50-pk.<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/25846\">\u67e5\u770b\u4ea7\u54c1<\/a>\n                                                    <\/div>\n                    <\/div>\n                                    <div class=\"cpb-item\">\n                        <div class=\"cpb-col cpb-col--left\">\n                            <a class=\"cpb-catalog\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/23984\">                                \u8d27\u53f7\uff1a 23984                            <\/a>                        <\/div>\n                        <div class=\"cpb-col cpb-col--middle\">\n                            <div class=\"cpb-title\">30 mm Syringe Filter, 0.22 \u00b5m, PTFE, White, 100-pk.<\/div>\n                        <\/div>\n                        <div class=\"cpb-col cpb-col--right\">\n                                                            <a class=\"cpb-view-btn\" target=\"_blank\" rel=\"noopener noreferrer\" href=\"https:\/\/www.restek.com\/p\/23984\">\u67e5\u770b\u4ea7\u54c1<\/a>\n                                                    <\/div>\n                    <\/div>\n                            <\/div>\n        <\/div>\n        \n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>This fast, simple LC-MS\/MS workflow for the direct analysis of polar pesticides in spinach provides good retention and peak shape over hundreds of matrix injections on a Raptor Polar X 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