The cannabis industry is always innovating with a constant revolving door of hot new products. In the past we\u2019ve seen buzz around products containing delta-8-THC, delta-10-THC, and THCP. <\/p>\n\n\n\n
Recently there has been a lot of interest in tetrahydrocannabinol acetate, or THC-O acetate. THC-O acetate is a psychoactive synthetic compound produced by acetylating THC. As far as legality, there is arguably some grey area and varies by state. There are claims of THC-O acetate being stronger than delta-9-THC and many products containing THC-O acetate are becoming available on the market. As such, labs are interested in including this compound into their cannabinoid potency methods.<\/p>\n\n\n\n
Herein, three methods have been developed to monitor 12 cannabinoids, including delta-10-THC and THC-O acetate by LC-UV. Since labs have different requirements, different methods were developed so the user has the flexibility to use an unbuffered or a buffered mobile phase system as well as methanol or acetonitrile in order to best fit their needs. For this analysis, a Raptor ARC-18 150 x 3 mm, 2.7 \u00b5m column was chosen to achieve desired resolution while keeping the analysis time under 12 minutes.<\/p>\n\n\n\n
Figure 1<\/strong>. Method conditions and extracted chromatogram for the analysis of 13 cannabinoids by LC-UV using acetonitrile as an organic modifier and buffered aqueous mobile phase.<\/p>\n\n\n