Bile acids are a group of steroidal acids with carboxyl and hydroxyl groups on the side. They are the major metabolic products of cholesterol. Bile acids play important roles as biomarkers for early diagnosis and therapeutic monitoring of many diseases,…
My first blog here at Restek was a demonstration of how increasing the source temperature could improve the raw response of 2,4-dinitrophenol while maintaining the relative response. Four years later I’m here with the promised follow-up on the elevated source…
Recently one of the designers of the Super-Clean Gas Filters spent some time with technical service and we were able to ask him a few common questions we get asked by customers. I thought I would share this information with…
Recently I came across a situation where I wanted to make a series of constant flow GC-FID runs, during which, the inlet pressure would increase from 2 to 6 PSI over the temperature program. I also wanted to use split…
Peak Capacity in capillary gas chromatography is different than sample loading capacity, which is something I’ve posted on multiple times recently in ChromaBLOGraphy. Peak capacity is simply the number of theoretical peaks that can “fit” inside a chromatogram under some…
You’re ready to install a column into your GC and realize you do not have ferrules to do the job. But which ferrule do you choose? The parameters to consider when choosing the correct ferrule are instrument make and model,…
The first time I was asked by a customer about how to convert % by weight of one of our FAME reference standards to µg/mL, I needed to ask for some help. Because we (tech service) occasionally get asked this…
Well, it’s partly a matter of semantics sometimes, but there is usually nothing drastic that you need to do to get your HPLC column going if it is new. There is no baking at high temperatures involved like you…
Molecular Sieve 5A & 13X Columns (packed) Molecular Sieve 5A & 13X (micropacked) Things to Consider Before Ordering a Packed Column . Following the steps below will ensure that your Molecular Sieve packed column is ready to use just several…
When purchasing a GC column the length that you buy isn’t necessarily the exact length that you get. In fact, it is typical that you will receive 0.5 to 1.0 meter more than what you bought! Knowing the exact length…
