Whether you are using fully porous silica or SPP silica, some equilibration time is needed between runs if you are using a gradient, and the amount of time is similar for both types of columns. Usually, the equivalent of seven column (void) volumes is sufficient unless you are using an ion-pairing technique.
How much equilibration time is required in between gradient runs on a Raptor Biphenyl column?
31 Jul 2025
Author
GNOT5062
