Use an inert column like a Raptor Inert Biphenyl and check your instrument manufacturer for non-stainless-steel parts that you can incorporate into the unit. PEEK, MP35N alloy, and ceramic components are great solutions to mitigate nonspecific binding.
I do not have an inert HPLC instrument; what are some of the configurations that I should implement to minimize nonspecific binding?
01 Aug 2025
Author
GNOT5103
