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PFAS Analysis by U.S. USDA CLG-PFAS 2.04 for Bovine, Porcine, Poultry, Siluriformes Muscle, and Bovine Plasma

03 Oct 2025

feature FSAR4290
  • Sample Matrix: bovine, porcine, poultry, and Siluriformes muscle and bovine plasma
  • Sample Preparation: protein precipitation extraction
  • Detection Technique: UHPLC-MS/MS

Screening, Determination, and Confirmation of PFAS by UHPLC-MS-MS

USDA Method CLG-PFAS 2.04 is used for the analysis of 16 PFAS in bovine, porcine, poultry, Siluriformes muscle samples, and in bovine plasma samples. It combines a methanol-based protein precipitation extraction with UHPLC-MS/MS analysis on a C18 column. The method is performance based and allows equivalent equipment, reagents, or solutions to be used, and some instrument parameters can be optimized as detailed in the method to improve chromatography and detection. The full analyte list and recommended products for PFAS analysis in bovine, porcine, poultry, Siluriformes muscle, and bovine plasma samples by CLG-PFAS 2.04 are provided below. In addition, you can register and directly compare analyte lists and matrices across PFAS analysis methods here or visit www.restek.com/PFAS to explore our technical resources for PFAS analysis.

Analyte List for USDA CLG-PFAS 2.04 for PFAS Analysis

PFAS acronyms, compound names, and CAS numbers vary across methods and guidelines, and they also depend on compound form (e.g., neutral vs. salt). The following list uses standardized terminology to allow easy comparison across the methods and guidelines in our comprehensive guide. Please refer to U.S. USDA CLG-PFAS 2.04 for method-specific nomenclature.

AcronymCompound NameCAS#
PFBSPerfluoro-1-butanesulfonic acid375-73-5
PFDAPerfluorodecanoic acid335-76-2
PFDoAPerfluorododecanoic acid307-55-1
PFHpAPerfluoroheptanoic acid375-85-9
PFHxSPerfluoro-1-hexanesulfonic acid355-46-4
PFHxAPerfluorohexanoic acid307-24-4
PFNAPerfluorononanoic acid375-95-1
PFOSPerfluorooctanesulfonic acid1763-23-1
PFOAPerfluorooctanoic acid335-67-1
PFTeDAPerfluorotetradecanoic acid376-06-7
PFTrDAPerfluorotridecanoic acid72629-94-8
PFUnAPerfluoroundecanoic acid2058-94-8
PFPeAPerfluoropentanoic acid2706-90-3
PFDSPerfluoro-1-decanesulfonic acid335-77-3
PFHxDAPerfluoro-n-hexadecanoic acid67905-19-5
PFODAPerfluoro-n-octadecanoic acid16517-11-6

Recommended Products for U.S. USDA CLG-PFAS for PFAS Analysis

Analytical LC Columns:


Raptor C18, 2.7 µm, 50×2.1 mm HPLC-Säule
Force C18, 1.8 µm, 50 x 2.1mm LC-Säule

Delay Columns:


PFAS Delay-Säule, 5 µm, 50×2.1 mm HPLC-Säule

PFAS delay column: 5 µm, 50 x 3.0 mm (cat.# 580959 [custom product])

Guard Columns:


UltraShield UHPLC Vorsäulen-Filter, 0.2 μm Fritte, 1
EXP Direct Connect-Halter für EXP Vorsäulenkartuschen, enthält Fittings und Ferrules
Raptor C18 EXP Vorsäulenkartusche, 2.7 µm, 5 x 2.1 mm, 3er Pack

Standards:


EPA 537.1 PFAS Kalibrierstandard, 2 µg/mL, Methanol (1 mM KOH), 1 mL/Ampulle
Discontinued: EPA 533 PFAS Kalibrierstandard, 2 µg/mL, Methanol (1 mM KOH), 1 mL/Ampulle

Sample Prep:


Q-sep Multispeed-Zentrifuge für QuEChERS

Sample Reservoirs:


SPE Leerröhrchen, Probenreservoir, 75 mL, Polypropylen, 12er Pack
SPE Verbinder, 1, 3, 6, 10 oder 15 mL, Polypropylen, 15er Pack

Vacuum Manifolds:


Resprep QR-24 Vakuum-Absaugstation
Resprep QR-12 Vakuum-Absaugstation

Vacuum Pumps:


Chemker 300 PTFE Vakuumpumpe, 20 L/min, AC 110 V, 60 Hz
Chemker 300 PTFE Vakuumpumpe, 18 L/min, AC 220 V, 50 Hz

General Lab Supplies:


Screw-Thread Polypropylen Vials mit begrenztem Volumen, 9 mm, 700 μL, 12 x 32 mm, 100er Pack
Screw-Thread Polypropylen Vials mit begrenztem Volumen, 9 mm, 700 μL, 12 x 32 mm, 1000er Pack
2.0 mL, 9 mm Solid Top-Polyethylen Kappen, Screw-Thread, 10 mil dicke Membran, farblos, 100er Pack
2.0 mL, 9 mm Solid Top-Polyethylen Kappen, Screw-Thread, 10 mil dicke Membran, farblos, 1000er Pack
Leere Zentrifugenröhrchen, 50 mL, Polypropylen mit Kappe, 50er Pck.
Leere Zentrifugenröhrchen, 50 mL, Polypropylen mit Kappe, 500er Pck.
Norm-Ject Kunststoffspritze, 10 mL Luer-Lock-Spitze, 100er Pck.
Norm-Ject Kunststoffspritze, 5 mL Luer-Lock-Spitze, 100er Pck.

Discover More Solutions for PFAS Analysis!

EZLC modeler PFAS Placeholder Image

Author

  • Restek Corporation

    Restek is a leading provider of chromatography columns, accessories, and certified reference materials. Trust Restek for reliable, high-quality analytical solutions.

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