Defined PressFit connectors and Press-Tight connectors are easy to use, but if they are not properly sealed, they can loosen due to thermal expansion during temperature-programmed runs. The keys to successful sealing are (1) making a clean, square cut on the column, and (2) moistening the end of the column with methanol or acetonitrile before seating it into the connector. A small amount of polyimide resin also helps prevent the seal from separating during temperature cycling.
For complete instructions on using the Press-Tight connector, consult the document included with the product or reference the Universal Fused Silica Press-Tight Connectors instruction sheet here.
