Per- and Polyfluoroalkyl substances(PFAS) have been a trending global issue for a number of years now. As more research is being carried out on these compounds, their breakdown paths, and their bioavailability, additional methods are required to quantify at lower concentrations, and to look for novel chemistries. As this search has expanded, shorter chain, and particularly ultrashort chain (USC) PFAS with carbon backbone lengths shorter than C4 have become areas of focus. In the most recent EU Commission Notice for Drinking Water (C/2024/4910) the fact TFA was not included in their definition of “PFAS Total” is highlighted as potential cause for concern, and both TFA and USC PFAS more generally are highlighted as areas for more specific monitoring. In this poster we examine two separate approaches to examining USC PFAS compounds. The first is a comprehensive look at a panel of PFAS compounds (C1-C14) with a dilute and shoot method using an inert coated, polar embedded alkyl LC column. The second is a more targeted screen via direct injection, focused upon USC PFAS utilising a novel HILIC/ion exchange LC column. These methods were then confirmed utilising a variety of fortified potable and non-potable water samples (industrial effluents, along with bottled, tap and spring waters). Both workflows demonstrate accuracy, sensitivity and linear calibration ranges of ppt levels, within industrially acceptable recovery values (70-130%). By integrating innovative tools and versatile columns, laboratories can streamline operations, save resources, and support sustainability—all without compromising analytical quality.

Analytical labs face growing demands to reduce energy use, solvent waste, and costs without sacrificing performance. Traditional workflows often rely on time-consuming sample prep and multiple methods, increasing environmental impact and complexity. Our solution combines virtual method development tools and advanced chromatography phases to create greener, more efficient workflows. Tools like the Pro EZLC Chromatogram Modeler and Method Translator enable remote optimization and modernization of methods, cutting instrument time, solvent use, and energy consumption. Key to multi-method development is selecting the right stationary phase. While conventional C18 phases struggle with diverse analytes, Biphenyl phases offer enhanced selectivity through π–π and hydrophobic interactions, allowing consolidation of multiple analyses on a single column. This simplifies workflows, reduces material costs, and minimizes errors. By integrating innovative tools and versatile columns, laboratories can streamline operations, save resources, and support sustainability—all without compromising analytical quality.